Unknown DNA â Rational Primer Design and Analysis â the ...
Unknown DNA â Rational Primer Design and Analysis â the ...
Unknown DNA â Rational Primer Design and Analysis â the ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
product length: 1360<br />
product %GC: 41.1<br />
product Tm: 76.3 degrees Celsius<br />
difference in primer Tm: 1.9 degrees Celsius<br />
annealing score: 58<br />
optimal annealing temperature: 54.8 degrees Celsius<br />
--------------------------------------------------------------------------------<br />
Product: 3<br />
[<strong>DNA</strong>] = 50.000 nM<br />
[salt] = 50.000 mM<br />
PRIMERS<br />
-------<br />
forward primer: forward2<br />
reverse primer: reverse13<br />
5' 3'<br />
forward primer (27-mer): 43 GTTATTGGTCACGTTGATTCAGGTAAG 69<br />
reverse primer (25-mer): 1427 TGCTTCATATCACGAACAGCGAAAC 1403<br />
forward<br />
reverse<br />
primer %GC: 40.7 44.0<br />
primer Tm (degrees Celsius): 54.7 56.5<br />
PRODUCT<br />
-------<br />
product length: 1385<br />
product %GC: 41.2<br />
product Tm: 76.3 degrees Celsius<br />
difference in primer Tm: 1.8 degrees Celsius<br />
annealing score: 59<br />
optimal annealing temperature: 54.9 degrees Celsius<br />
--------------------------------------------------------------------------------<br />
Product: 4<br />
[<strong>DNA</strong>] = 50.000 nM<br />
[salt] = 50.000 mM<br />
PRIMERS<br />
-------<br />
forward primer: forward15<br />
reverse primer: reverse1<br />
5' 3'<br />
forward primer (29-mer): 62 CAGGTAAGTCAACTACTACTGGTCACCTC 90<br />
reverse primer (25-mer): 1420 TATCACGAACAGCGAAACGACCGAG 1396<br />
forward<br />
reverse<br />
primer %GC: 48.3 52.0<br />
primer Tm (degrees Celsius): 57.9 59.4<br />
PRODUCT<br />
-------<br />
product length: 1359<br />
product %GC: 41.2<br />
product Tm: 76.3 degrees Celsius<br />
30
Change language