in rabies - libdoc.who.int - World Health Organization
in rabies - libdoc.who.int - World Health Organization
in rabies - libdoc.who.int - World Health Organization
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LABORATORY TECHNIQUES IN RABIES<br />
Preparation of slides<br />
Tlie Negri body: differential diagnosis<br />
The mouse <strong>in</strong>oculation test<br />
Annex Pre~aration of Sellers' sta<strong>in</strong><br />
Chapter 5 Histopathological diagnosis (P Lep<strong>in</strong>e & P. Atanasiu) 66<br />
Removal of the bra~n and preparation of tissue samples for<br />
exam<strong>in</strong>ation 68<br />
Ernbedciirig, sh<strong>in</strong><strong>in</strong>g and exam<strong>in</strong>ation for Negri bodies 77<br />
Chapter 6<br />
Ctiapter 7<br />
The lnouse <strong>in</strong>oculation test (H. Koprowsk~)<br />
Choice of mice<br />
Preparation of suspect material for rnoculation<br />
Inoculation of mice<br />
Observation of <strong>in</strong>oculated mice<br />
Further passages of <strong>in</strong>fected material<br />
Removal of tile bra<strong>in</strong><br />
Complications<br />
The fluorescent antibody test (D J Dean M K Abelseth &<br />
P Atanas~u) 88<br />
Pr<strong>in</strong>ciple 88<br />
Materials and methods 89<br />
Discussion 93<br />
Chapter 8 Virus isolation <strong>in</strong> neuroblastorna cell culture A. Webster &<br />
G. A. Casey) 96<br />
Rabies tissue-culture <strong>in</strong>fection test (RTCIT) 97<br />
References 101<br />
Annex l Media 102<br />
Annex 2 Avid<strong>in</strong>-biot<strong>in</strong> sta<strong>in</strong><strong>in</strong>g method 103<br />
Chapter 9<br />
Rapid <strong>rabies</strong> enzyme irnmunodiagnosis (RREID) for rab~es<br />
ant~gen detection (H Eourhy & P Perr<strong>in</strong>) 105<br />
Introduction 105<br />
Method 105<br />
Evaluation of the technique 111<br />
References 111<br />
Annex Preparation of reagents 112<br />
Chapter 10 Cell culture of <strong>rabies</strong> virus (A A K<strong>in</strong>g)<br />
Susceptible cells, cell lhnes and stra<strong>in</strong>s<br />
Methods of virus propagation<br />
Cytopathology<br />
Persistent <strong>in</strong>fection<br />
Virus <strong>in</strong> <strong>in</strong>tected cells<br />
Application of cell-culture methods<br />
References