in rabies - libdoc.who.int - World Health Organization

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LABORATORY TECHNIQUES IN RABIES Preparation of slides Tlie Negri body: differential diagnosis The mouse inoculation test Annex Pre~aration of Sellers' stain Chapter 5 Histopathological diagnosis (P Lepine & P. Atanasiu) 66 Removal of the bra~n and preparation of tissue samples for examination 68 Ernbedciirig, shining and examination for Negri bodies 77 Chapter 6 Ctiapter 7 The lnouse inoculation test (H. Koprowsk~) Choice of mice Preparation of suspect material for rnoculation Inoculation of mice Observation of inoculated mice Further passages of infected material Removal of tile brain Complications The fluorescent antibody test (D J Dean M K Abelseth & P Atanas~u) 88 Principle 88 Materials and methods 89 Discussion 93 Chapter 8 Virus isolation in neuroblastorna cell culture A. Webster & G. A. Casey) 96 Rabies tissue-culture infection test (RTCIT) 97 References 101 Annex l Media 102 Annex 2 Avidin-biotin staining method 103 Chapter 9 Rapid rabies enzyme irnmunodiagnosis (RREID) for rab~es ant~gen detection (H Eourhy & P Perrin) 105 Introduction 105 Method 105 Evaluation of the technique 111 References 111 Annex Preparation of reagents 112 Chapter 10 Cell culture of rabies virus (A A King) Susceptible cells, cell lhnes and strains Methods of virus propagation Cytopathology Persistent infection Virus in intected cells Application of cell-culture methods References
CONTENTS Part Ill. Special diagnostic and research techniques Chapter 11 Techniques for the production, screering and characterization of rnonoclonal antibodies (M, Lafon) Immunization of animals Myelomas Fetal calf sera Fusion Screening of hybrdoma supernatants Cloning of hybridomas by lim~ling dili~tion Prodi~ction of large n~nounts of rnonnclonal antibodies Freezing and thawing of hybridomas Characterization of rnonoclonal antibodies Use of rnonoclonal antibodies References Aniiex Dulbeccos modified Eagle's medium (DMEM) Chapter 12 Monoclonal antibodies for the identification of rabies and non-rabies lyssaviruses (J. S. SmitA & A. A. King) Materials and methods lmmunofliiorescence tests using MAb-RNPs Antigenic analysis using MAb-Gs Applications Discussion References Chapter 13 The polynlerase chain reaction (PCR) technique for diagnosis. typing and epidemiological studies of rabies (N. Tordo, D. Sacrament0 & H. Bourhy) Introduction Amplification of the rabies transcripts Diagnosis Typing and molecular epidemiological studies References Annex Preparation of buffers and reagents Chapter 14 Techniques for the purification of rabies virus, its subunits and recornbinaril products (B. Dietzschold) Introduction Pcrrificatiori of rabies virus particles Purification of rabies virus siibunits and structural proteins under non-denaturing conditions Purification of rabies virus proteins under denaturing coriditions References Annex Preparation of reagents Chapter 15 A rapid fluorescent focus inhibition test (RFFIT) for determining rabies virus-~ieiitralizing antibody (J. S. Smith. P. A. Yager & G. M. Baer)
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CHARACTERlSTlCS AND MOLECULAR BIOLO
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CHAPTER 4 Rapid microscopic examina
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EXAMINATION FOR NEGRl BODIES Fig. 4
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