in rabies - libdoc.who.int - World Health Organization

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LABORATORY TECHNIQUES IN RABIES Standard procedure Calcu!at~on of virus-neutralizing antibody titres Alternative test procedures Interpretation of results References Annex 1 Growth media for MNA and BHK-21 S13 cells Annex 2 Calculation of titres Chapter 16 An in vltro virus neutralization test for rabies antibody (C. V Triinarchi. R. D. Rudd & M. Saffoid. Jr) Method Interpretation of results References Chapter 17 Competitve ELISA for the detection of rabies virus-neutralizing antibodes (L. D. Eimgren & A. l. Wandeler) Method Interpretation of results References Annex 1 Preparaion of buffers and reagents Annex 2 Conjugaton of monoclona! antibodies Annex 3 ELISA software Chapter 18 Electron microscopy (K. Hummeler & P. Atanasiu) Structural studes Studies of morphogenesis Annex Medium for agarose: Eagle's basal medium (EBM) Part IV. Methods of vaccine production Section A. Brain-tissue vaccines 221 Chapter 19 General consideratons in the production and use of braintissue and purified chicken-embryo rabes vaccines for human use (F.-X. Meslin & M. M. Kaplan) 223 Introduction 223 Adverse efTects of brain-tissue vacclnes 224 Recent developments in brain-tssue vaccine production 226 References 228 Chapter 20 P-Propolactone-inactivated sheep brain vaccine (H. Sir7gii) 234 Cornpositon 234 Preparation of the seed vrus 234 Preparation of the vaccine 235 Quality control tests 237 Biochemical tests 238 Preparation of standard vaccine 240 Dosage schedule 240 References 241 Annex 1 Preparation of 0.5 mol/l sodium-potassium phosphate buffer, pH 7.6 24 1
CONTENTS Annex 2 Preparation of 0.05 rnol/l phosphate-buffered saline (PBS), pH 7 0 Annex 3 Preparation of stabilizer for rabies vaccine, pH 7.2 Chapter 21 Suckl~ng-mouse brain vaccine (A M Dlaz) Formilla Preparat~on of the inoculum Inoculation and harvest Preparaton of the vaccine Control tests Expiry date References Annex 1 Ultraviolet irradiation for inactivation of vaccines Annex 2 Pre~aration of stabilizer solutions Section B. Ernbryonating egg vaccines Chapter 22 Purified duck-embryo vaccine for Run~ans (R. Gluck) Preparation of the vaccine Control tests Expiry date References Annex Preparation of stabilizing medium Chapter 23 Chicken-embryo vaccine for dogs (H. Koprowski) Preparation of the v- =ccine Control tests References Annex Preparation of stabilizing solution, pH 7.6 Section C. Cell-culture vaccines Chapter 24 Cell-culture vaccines for Iiunian use general considerations (K G Nicholson) Human diploid cell vaccine Other cell-culture vaccines Safety Efficacy Economical post-exposure treatment regimens Pre-exposure ~mrnun~zation References Chapter 25 Vaccine for humans prepared in human diploid cells (R. Brarictie) Preparation of the vaccine Contl-ol tests Conclusion References Annex Flow chart for the production of HDC vaccine using the MRC5 cell strain
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fig. 3.5 Wild isolates of rabies (g
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LABORATORY TECHNIQUES IN RABIES the
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LABORATORY TECHNIQUES IN RABIES One
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CHARACTERlSTlCS AND MOLECULAR BIOLO
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CHARACTERISTICS AND MOLECULAR BIOLO
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CHAPTER 4 Rapid microscopic examina
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EXAMINATION FOR NEGRl BODIES Fig. 4
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EXAMINATION FOR NEGRl BODIES Smear
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EXAMINATION FOR NEGRl BODIES Fig. 4
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