diversity of Cylindrocarpon species - Australasian Plant Pathology ...
diversity of Cylindrocarpon species - Australasian Plant Pathology ...
diversity of Cylindrocarpon species - Australasian Plant Pathology ...
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Objective 2: Characterising genetic <strong>diversity</strong> <strong>of</strong><br />
the three predominant <strong>Cylindrocarpon</strong> spp.<br />
Selection <strong>of</strong> UP-PCR Primers:<br />
• 5 out <strong>of</strong> 11 chosen<br />
• 3 test isolates<br />
• Chosen for # polymophisms & band<br />
distribution<br />
UP-PCR using AA2M2 primer for<br />
C. liriodendri<br />
DNA fingerprints were generated for<br />
151 isolates using UP-PCR:<br />
• C. liriodendri (57) , C. macrodidymum<br />
(41) , C. destructans (53)<br />
Neighbour Joining Tree:<br />
• ~ 70 data points per isolate<br />
• Generated using PAUP s<strong>of</strong>tware<br />
• Identified main genetic groups
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