diversity of Cylindrocarpon species - Australasian Plant Pathology ...

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Identification of Cylindrocarpon species infecting New Zealand grapevines Identification by morphological features was insufficient to differentiate the species Process of molecular identification Genomic DNA was extracted from mycelium
MW C. macrodidymum C. liriodendri C. destructans ‐ve Amplification with species specific primers CymaF1 & CymaR1 Species Specific Primers: • Lizel Mostert, Stellenbosch University C. macrodidymum – CymaF1 & CymaR1 = 300 bp C. liriodendri – CyliF1 & CyliR1 = 200 bp • Hayley Ridgway, Lincoln University C. destructans – CydeF1 & CydeR1 = 200 bp PCR products from the tubulin gene sequenced: -10% of isolates from sp-sp PCR - 14 unidentified isolates
Page 1 and 2: Species composition and genetic div
Page 3 and 4: Symptoms of Black foot disease Chlo
Page 5 and 6: Photo by Francois Halleen Vascular
Page 7: Objective 1: To determine the popul
Page 11 and 12: Objective 1: Results - 41 isolates
Page 13 and 14: Objective 2:C. macrodidymum • NJ
Page 15 and 16: C. liriodendri • NJ tree produced
Page 17 and 18: Acknowledgements • Carolyn Bleach

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