diversity of Cylindrocarpon species - Australasian Plant Pathology ...

MW C. macrodidymum C. liriodendri C. destructans ‐ve
Amplification with species specific primers CymaF1 & CymaR1
Species Specific Primers:
• Lizel Mostert, Stellenbosch University
C. macrodidymum – CymaF1 & CymaR1 = 300 bp
C. liriodendri – CyliF1 & CyliR1 = 200 bp
• Hayley Ridgway, Lincoln University
C. destructans – CydeF1 & CydeR1 = 200 bp
PCR products from the tubulin gene sequenced:
-10% of isolates from sp-sp PCR
- 14 unidentified isolates