datasheet - Dolomite Microfluidics

The Dolomite Centre Ltd 

Mitos P-Pump Accessories 

product datasheet 

page 

Flow Resistors 3 

Flow Resistor Selection 6 

Flow Resistor Kit 7 

Mitos P Pump Vessel Holder Kit 9 

Standard Vessel Compatibility Chart 11 

Mitos P Pump Starter Kit 13 

Pneumatic Connector Kit 14 

Mitos P Pump 3-way Chamber Kit 15 

Mitos P Pump 3-way Vessel Holder Kit 16 

Mitos P Pump Remote Chamber 400 17 

Mitos P-Pump Remote Chamber 30 18 

Mitos P-Pump Remote Chamber with 

3-way Accessories 

19 

Fluid Tube (1/16”) to Pneumatic Tube (6mm) 

Adaptor 

20 

Dip Tube Fitting 20 

USB to RS232 Adaptor Cable 

21 

MAR-000029 1.5 Page 1 of 22 

(Part No. 3200197)


Part name 

Part number 

Flow Resistors 3200265-3200271 

Flow Resistor Kit 3200272 

Mitos P-Pump Vessel Holder Kit 3200017 

Mitos P-Pump Starter Kit 3200033 

Pneumatic Connector Kit 3200034 

Mitos P-Pump 3-way Chamber Lid 3200044 

Mitos P-Pump 3-way Vessel Holder Kit 3200045 



Mitos P-Pump Remote Chamber with 3-way Accessories 3200206 

Dip Tube Fitting 3200143 

Fluid Tube (1/16”) to Pneumatic Tube (6mm) Adaptor 3200301 

RS232 Serial Extension Cable 3200239 

RS232 to USB Adaptor Module 3200197 

MAR-000029 1.5 Page 2 of 22


Flow Resistors (Part No. 3200265-3200271) 

Flow Resistors are high tolerance lengths of PEEK covered fused silica tubing that 

provide a known flow rate at a selected pressure. They can be used to insert additional 

flow resistance into a system and are designed for use with the Mitos P-Pump range. 

A Flow Resistor enables very low flow rates to be achieved when connecting to a Mitos 

P-Pump to systems of low fluidic resistance. For applications such as droplet generation, 

a Flow Resistor can be used to prevent flow rate instability due to the varying back 

pressure generated by using immiscible fluids of different viscosities. Dolomite offers a 

range of Flow Resistors to optimise your microfluidic system. 

3200267 - Flow Resistor F1 

system 

Flow resistor connected between a Mitos P-Pump and system 

MAR-000029 1.5 Page 3 of 22


Benefits 

Allows the flow rate range of the Mitos P-Pump to be maximized 

Enables the user to operate at very low flow rates with the Mitos P-Pump 

Enables the use of very low viscosity liquids (e.g. gases) with the Mitos P-Pump 

Prevents flow rate instability when using fluids of different viscosities 

Two Flow Resistors can be used to provide equal flow splitting into two channels 

Quick and easy to connect using included fittings 

Excellent chemical compatibility 

Specifications 

Specification 3200265 

F0.1 

Approx. flow 

rate generated 

by water 

pumped at 

1bar pressure 

3200266 

F0.3 

3200267 

F1 

3200268 

F3 

3200269 

F10 

3200270 

F30 

3200271 

F100 

0.1µl/min 0.3µl/min 1µl/min 3µl/min 10µl/min 30µl/min 100µl/ 

min 

Max flow rate 1 1µl/min 3µl/min 10µl/min 30µl/min 100µl/ 

min 

300µl/ 

min 

1000µl/ 

min 

Min flow rate 2 

Internal 

diameter 

Internal 

diameter 

tolerance 

Outer diameter 

Wetted 

materials 

Sheath 

materials 

0.002µl/ 

min 

0.006µl/ 

min 

0.02µl/mi 

n 

0.06µl/mi 

n 

0.2µl/min 0.6µl/min 2µl/min 

25µm 50µm 75µm 100µm 

± 1µm ± 2µm ± 3µm ± 3µm 

1/16” (1.59mm) 

Fused silica 

Polyimide (inner sheath) 

PEEK (outer sheath) 

1) Maximum flow rate is the approximate flow rate produced with water when the Mitos P- 

Pump is set to 10bar and there is little or no fluidic resistance downstream of the flow 

restrictor. 

2) Minimum flow rate is the approximate flow rate produced with water when the Mitos P- 

Pump is set to 20mbar and there is little or no fluidic resistance downstream of the flow 

restrictor. 

MAR-000029 1.5 Page 4 of 22


Flow accessories 

The Flow Resistors are supplied with the following flow accessories: 

2 x Compact Head Fittings (ETFE) with 1/4 - 28” thread for connection to 1/16” 

OD pipe, can be used with the Flangeless Ferules. 

2 x Flangeless Ferules (ETFE) for connection to 1/16” OD pipe. To be used with 

the Compact Head Fittings. 

2 x Straight Female Couplings (Polypropylene) with female to female 1/4 - 28” 

threads. The couplings are used for making connection between Compact Head 

Fittings. 

1 x Ferule (PCTFE) with Integrated Filter (stainless steel, 2 µm pore size). To be 

used with 1/16” OD pipe. 

Each Flow Resistor is supplied with these flow accessories to enable the Flow Resistor to 

be connected in-line. The included filter can be used to prevent blockage of the small 

internal diameter of the flow resistor. 

Flow resistors can also be directly connected to a microfluidic chip using the Multiflux® 

Linear Connectors. 

Each Flow Resistor is supplied with the above flow accessories 

to enable the Flow Resistor to be connected in-line. 

MAR-000029 1.5 Page 5 of 22

Dynamic viscosity of selected fluid (cP) 


Flow Resistor Selection 

The Flow Resistor should be selected based on the desired flow rate and the viscosity of 

the selected fluid using the table below. This assumes that the flow resistance of the 

system is low in comparison with the Flow Resistor. 

0.1 

e.g. propane 

gas 

at 20ºC 

0.3 

e.g. acetone 

at 20 ºC 

0.1 

Flow rate 

range 

0.002 – 1 

Desired flow rate at 1bar pressure (µl/min) 

0.3 

Flow rate 

range 

0.006 – 3 

1 

Flow rate 

range 

0.02 – 10 

3 

Flow rate 

range 

0.06 – 30 

10 

Flow rate 

range 

0.2 – 100 

30 

Flow rate 

range 

0.6 – 300 

100 

Flow rate 

range 

2 – 1000 

- - F0.1 F0.3 F1 F3 F10 

- F0.1 F0.3 F1 F3 F10 F30 

1 

e.g. water 

at 20ºC 

3 

blood 

at 37ºC 

10 

25% wt CaCl 

sol. 

at 20ºC 

30 

vegetable oil 

at 40ºC 

100 

light machine 

oil 

at 20ºC 

F0.1 F0.3 F1 F3 F10 F30 F100 

F0.3 F1 F3 F10 F30 F100 

F1 F3 F10 F30 F100 

F3 F10 F30 F100 

F10 F30 F100 

Operate without 

flow resistors 

MAR-000029 1.5 Page 6 of 22


Flow Resistor Kit (Part No. 3200272) 

Designed for the Mitos P-Pump range the Flow Resistor Kit can be used to insert 

additional flow resistance into a system. The Flow Resistors enable very low flow rates to 

be achieved when connecting to a Mitos P-Pump to systems of low fluidic resistance. 

For applications such as droplet generation, a Flow Resistor can be used to prevent flow 

rate instability due to the varying back pressure generated by using immiscible fluids of 

different viscosities. 

Flow Resistor Kit (Part No. 3200272) 

Benefits 

Allows the flow rate range of the Mitos P-Pump to be maximised 

Enables the user to operate at very low flow rates with the Mitos P-Pump 

Enables the use of very low viscosity liquids (e.g. gases) with the Mitos P-Pump 

Prevents flow rate instability when using fluids of different viscosities 

Quick and easy to connect using included fittings 


MAR-000029 1.5 Page 7 of 22


The Flow Resistor Kit contains the following flow resistors: 

Item Description Qty 

1 Flow Resistor F1 Min flow rate: 0.02µl/min, Max flow rate: 10µl/min 1 




5 Flow Resistor F100 Min flow rate: 2µl/min, Max flow rate: 1000µl/min 1 

Each flow resistor kit also contains 5 sets of flow accessories to enable the flow resistors 

to be connected in-line and the fluids to be filtered before entry into the flow resistor. 

Each Flow Resistor is supplied with the above flow accessories 

to enable the Flow Resistor to be connected in-line. 

MAR-000029 1.5 Page 8 of 22


Mitos P-Pump Vessel Holder Kit (Part No. 3200017) 

The Mitos P-Pump Vessel Holders Kit contains 4 vessel holders to enable the Mitos 

P-Pump range to be used with a wide range of standard and non-standard fluid vessels. 

These include centrifuge tubes, auto sampler vials, scintillation vials, microreaction vials 

and other general purpose vials. The Vessel Holders can be easily positioned inside the 

pressure chamber and are quick to change. 

Left: Mitos P-Pump 

with vessel holder 2 

Right: Mitos P-Pump 

with vessel holder 4 

Vessel Holder 1 Vessel Holder 2 Vessel Holder 3 Vessel Holder 4 

MAR-000029 1.5 Page 9 of 22


Benefits 

Accommodate a wide range of standard and non-standard fluid vessels 

Ensures the Mitos P-Pump can be used with preferred vessel 

Minimize sample waste by pumping directly from your container 

Enable use of micro vials for low sample volumes 

Specification 

Vessel 

Holder 1 

Vessel 

Holder 2 

Vessel 

Holder 3 

Vessel 

Holder 4 

1 Hole diameter D1 (mm) 8 11 16.5 11 

2 Height H1 (mm) 25 35 40 15 

3 Base diameter D2 (mm) 15 20 27 27 

4 Height H2 (mm) 5 5 N/A N/A 

5 Material Stainless Steel 

MAR-000029 1.5 Page 10 of 22


Standard Vessel Compatibility Chart 

The compatibility chart contains some examples of commonly used vessels, which require 

a vessel holder for use with the Mitos P-Pump range. 

Standard Vessel Type 

Vessel Holder Required 

Abgene deep well cluster tube 0.3 ml 1 



Abgene flat cap tube 0.2 ml 1 

Autosampler vial 12 x 32 mm 1 

Autosampler vial 4 ml 1 

Chase culture tube 10 x 75 mm 4 

Chase culture tube 15 x 85 mm 3 

Chase scintillation vial 7 ml 2 



Chromacol universal storage vial 8ml 2 

MAR-000029 1.5 Page 11 of 22


Eppendorf micro centrifuge tube 0.5 ml 1 



Micro reaction vessel 0.1 ml 2 

Micro reaction vessel 0.3 ml 2 

Micro reaction vessel 1 ml 2 




Nalgene cryogenic vial 1.2 ml 1 

Nalgene cryogenic vial 2 ml 1 

Nalgene cryogenic vial 5 ml 1 

Nalgene oak ridge centrifuge tube 10 ml 3 

Nalgene sample vial 5 ml 3 



Wheaton sample glass vial 2 ml 1 



Wheaton v vial 0.3 ml 1 

Wheaton v vial 1 ml 1 



MAR-000029 1.5 Page 12 of 22


Mitos P-Pump Starter Kit (Part No. 3200033) 

The Mitos P-Pump Starter Kit contains a selection of vessels to enable sample liquids to 

be introduced into the range of Mitos P-Pumps. It also contains a selection of tubing and 

fittings to enable fluidic connection to be made between the P-Pump and your microfluidic 

system. 

Mitos P-Pump Starter Kit 

Benefits 

Enables the user to begin test work with the Mitos P-Pump immediately 

Contains a selection of vessels 

Contains a selection of tubing and fittings 

The Mitos P-Pump Starter Kit contains: 


1 30ml glass vessel 30ml, Ø28 x H83mm, R3 screw neck with cap, class 1 

5 

hydrolytic extraction neutral borosilicate glass 

2 20ml high recovery 20ml, Ø28 x H67mm, 24-400 screw thread with cap, class 1 2 

glass vessel hydrolytic extraction neutral borosilicate glass, tapered 

reservoir for high sample recovery 

3 FEP tubing 0.25mm ID, 1/16” (1.6 mm) OD, 10m length 1 

4 FEP tubing with 0.25mm ID, 1/16” (1.6 mm) OD, 1m length, ¼”-28 threaded 1 

attached fitting fitting with PTFE gripper attached to one end 

5 ¼”-28 fitting ¼”-28 threaded nut and ferule for 1/16” (1.6mm) OD tubing 5 

6 ¼”-28 union ¼”-28 female to female threaded union 1 

7 Tube cutter Double bevelled cutting blade produces clean 90º cut on 

polymer tubing up to 2mm OD 

1 

MAR-000029 1.5 Page 13 of 22


Pneumatic Connector Kit (Part No. 3200034) 

The Pneumatic Connector Kit contains a selection of fittings and tubing to enable 

connection of your compressed air or gas supply to the Mitos P-Pumps. 

Pneumatic Connector Kit 

Benefits 

Enables connection of most compressed air or gas supplies to the Mitos P-Pump 

Contains a selection of fittings and tubing 

The Pneumatic Connector Kit contains: 


1 Pneumatic tubing 6mm OD, 4mm ID, 5m length, polyurethane 1 

2 Straight connector 6mm Push-in tube – tube connector for 6mm OD tube 1 

3 Reducer 6 – 4mm 6mm male to 4mm female push-in tube connector 1 

4 Reducer 8 – 6mm 8mm male to 6mm female push-in tube connector 1 

5 T connector 6mm 6mm push-in 3-way tube connector 3 

6 Adaptor 6mm to 1/4” 6mm push-in tube connector to 1/4” BSPT male thread 1 

BSPT 

7 Adaptor 6mm to 1/8” 6mm push-in tube connector to 1/8” BSPP female thread 1 

BSPP 

8 Adaptor 1/4” NPT to 1/4” 1/4” NPT male thread to 1/4” BSPP female thread 1 

BSPP 

9 Adaptor 1/4” to 1/8” BSPT 1/4" push-in tube connector to 1/8” BSPT male thread 1 

MAR-000029 1.5 Page 14 of 22


Mitos P-Pump 3-way Chamber Lid (Part No. 3200044) 

The Mitos P-Pump 3-way Chamber Lid can be used to pump 3 liquids simultaneously 

from the Mitos P-Pump (Part No. 3200016). This provides a low cost method of delivering 

3 reagents to a microfluidic system with pulseless pressure pump technology. Also 

available, is a Mitos P-Pump 3-way Vessel Holder Kit (Part No. 3200045) to enable 3 

reagent vessels to be inserted into the chamber with ease. 

Mitos P-Pump 3-way Chamber Lid 

Mitos P-Pump with 3-way Chamber Lid and 3-way Vessel Holder 

Benefits 

Provides a low cost solution for 3-channel pumping with the Mitos P-Pump 

Push-and-rotate mechanism that is quick to open and close 

Safety features to ensure chamber is locked when pressurized 

Contains plugs to enable ports to be blocked when not required 

Specification 

Value 

1 Number of ports 3 

2 Chamber dimensions Ø30 x H 88mm 

3 Dip tube size 1/16” OD; 250µm ID 

4 Wetted materials User selected vessel and dip tube (e.g. glass 

and PTFE) 

5 Number of plugs included 10 

MAR-000029 1.5 Page 15 of 22


Mitos P-Pump 3-way Vessel Holder Kit (Part No. 3200045) 

The Mitos P-Pump 3-way Vessel Holder Kit is designed to accommodate 3 micro tubes 

in the Mitos P-Pump (Part No. 3200016). Included in the kit are compatible micro tubes 

with 1.5 ml volume. When used in conjunction with the 3-way Chamber Lid (Part No. 

3200044), it provides a low cost method of delivering 3 reagents to a microfluidic system 

with pulseless pressure pump technology. 

Mitos P-Pump 3-way Vessel Holder 

1.5 ml micro tube 

Mitos P-Pump with 3-way Chamber 

Lid and 3-way Vessel Holder 

Benefits 

Improves ease of use when using the Mitos P-Pump for 3-channel pumping 

Enables P-Pump to be used with 3 micro tubes 

Quick to install and remove 

Locating feature to align micro tubes with outlet ports in chamber lid 

Specification 

Value 

1 Vessel holder hole diameter D1 (mm) 11 

2 Vessel holder height H1 (mm) 15 

3 Vessel holder base diameter D2 (mm) 29 

4 Vessel holder material Stainless Steel 

5 Number of micro tubes 20 

6 Micro tube volume (ml) 1.5 

7 Micro tube material Polypropylene 

MAR-000029 1.5 Page 16 of 22


Mitos P-Pump Remote Chamber 400 (Part No. 3200043) 

The Mitos P-Pump Remote Chamber 400 is a 10bar stainless steel pressure chamber for 

use with the Mitos P-Pump (Part No. 3200016). It enables greater sample volumes up to 

400ml to be used, so that continuous pumping for longer periods of time is possible. With 

the precise pressure-driven pumping mechanism of the Mitos P-Pump, the flow from the 

chamber is highly stable and pulsefree. The remote chamber 400 is simple to connect 

and provides clear viewing of the sample liquid. For applications requiring temperature 

control and stirring, the remote chamber can be placed onto a hotplate/magnetic stirrer. 

3200043 - Mitos P-Pump 

Remote Chamber 400 

Mitos P-Pump (Part No. 3200016) with 

Remote Chamber 400 

Benefits 

Expands P-Pump capacity to liquid vessels up to 400ml 

Continuous pumping for long periods of time 

Option for heating and magnetic stirring 

Easy to view sample liquid levels 

Simple to connect to the P-Pump for precise pressure control 

250 ml DURAN vessel included 


Specification 

Value 

1 Pressure range 0 – 10bar (0 – 145psi) gauge 

2 Temperature limit 50ºC 

2 Chamber dimensions Ø82 x H 140mm 

3 Sample volume up to 400ml (with 400ml tall form beaker) 

4 Dip tube size 1/16” OD; 250µm ID 

5 Product size Ø148 x H 200mm 

6 Product weight 5.5kg 

7 Chamber materials Stainless Steel, Glass, Viton, PEEK 

8 Wetted materials User selected vessel and dip tube (e.g. glass and PTFE) 

Note: Pressure control performance will be as indicated in the P-Pump specification except response and settling times will 

be slower due to the increased volume 

MAR-000029 1.5 Page 17 of 22


Mitos P-Pump Remote Chamber 30 (Part No. 3200178) 

The Mitos P-Pump Remote Chamber 30 is a 0 – 10bar lockable glass pressure chamber 

for use with the Mitos P-Pump Remote (Part No. 3200176) and Mitos P-Pump Remote 

Basic (Part No. 3200177). It is easy to access and can accommodate samples from 100µl 

to 30ml. 

The Mitos P-Pump Remote Chamber 30 connects to a Mitos P-Pump Remote via a 

simple push-and-click action. For applications requiring temperature control and stirring, 

the remote chamber can be placed onto a hotplate, magnetic stirrer or placed in an 

incubator. 

Left: Mitos P-Pump Remote Chamber 30 

(Part No. 3200178) 

Below: Mitos P-Pump Remote Chamber 

30 (Part No. 3200178) connected to Mitos 

P-Pump Remote (Part No. 3200176) 

Benefits 

Sample volumes from 100µl to 30ml 



Quick and easy fitting to connect to Mitos P-Pump Remote 


MAR-000029 1.5 Page 18 of 22


Mitos P-Pump Remote Chamber with 3-way Accessories (Part No. 3200206) 

The Mitos Remote Chamber with 3-way Accessories is the Mitos P-Pump Remote 

Chamber 30 (Part No. 3200178) with Mitos P-Pump 3-way Chamber Lid (Part No. 

3200044) and Mitos P-Pump 3-way Vessel Holder Kit (Part No. 3200045), it provides a 

low cost method of delivering 3 reagents to a microfluidic system with pulseless pressure 

pump technology. 

3200178 - Mitos P-Pump Remote 

Chamber 30 

3200044 - Mitos P-Pump Basic 

with 3-way Chamber Lid 

3200045 - 3-way Vessel Holder Kit 

Benefits 

Sample volumes from 100µl to 30ml 



Quick and easy fitting to connect to Mitos P-Pump Remote 


MAR-000029 1.5 Page 19 of 22


Dip Tube Fitting (Part No. 3200143) 

Designed for use with the Mitos P-Pump (Part No. 3200016), the Dip Tube Fitting allows 

users to easily connect a dip tube into a fluid vessel on the Mitos P-Pump. The fitting 

features an in-built strain relief and is compatible with 1/16 OD tubing and 1/4- 28 flat 

bottom ports. 

Dip Tube Fitting (Part No. 3200143) 

Fluid Tube (1/16”) to Pneumatic Tube (6mm) Adaptor (Part No. 3200301) 

The Fluid Tube (1/16”) to Pneumatic Tube (6mm) Adaptor is used to convert 6mm air 

lines to 1/16” fluid tube, which is the tubing size required for connection to all Mitos P- 

Pumps. 

Fluid Tube (1/16”) to Pneumatic Tube (6mm) Adaptor (Part No. 3200301) 

MAR-000029 1.5 Page 20 of 22


USB to RS232 Adaptor Cable 

For PCs that do not support an RS232 serial interface, Dolomite offers a RS232 Serial 

Extension Cable (Part No. 3200239) and a RS232 to USB Adaptor Module (Part No. 

3200197) allowing users to connect their Mitos P-Pump Basic (Part No. 3200175) or 

Mitos P-Pump Remote Basic (Part No. 3200177) to a PC. 

Right: RS232 Serial Extension Cable (Part No. 3200239) 

Left: RS232 to USB Adaptor Module (Part No. 3200197) 

MAR-000029 1.5 Page 21 of 22


MAR-000029 1.5 Page 22 of 22

datasheet - Dolomite Microfluidics

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