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Bitter Apples For Cider Making - An Alternative

Bitter Apples For Cider Making - An Alternative

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Experimental<br />

Cultivar evaluations: Fruitlet samples were collected by cultivar. Some<br />

were sub-sampled into “large” or “small” fruitlets to assess the relative influence<br />

of size and date. Fruitlet samples were measured for average diameter, then<br />

weighed. 100 - 200 grams of the fruitlets were then cut into small pieces before<br />

grinding and mixing with a hand held “Bamix” unit. Grinding with, and without,<br />

metabisulphite at circa 300 mg / kg was trialed. A sample of the ground pulp was<br />

then placed in a disposable 10 mL syringe and approximately 2 mL of liquid<br />

collected through a 0.45 um, 25 mm dia, cellulose acetate filter disc.<br />

.<br />

Total Polyphenol levels were measured using a standard Fosters method<br />

(Costanzo, 2003) based on the ASBC and EBC International Method.<br />

The juice samples were diluted 10 fold by volume with distilled water by<br />

modifying the method and including 150 uL of sample instead of the usual 1500<br />

uL, and increasing the distilled water to make up the same volume<br />

The following aliquots were added to a disposable cuvette:<br />

• 800 uL CMC / EDTA reagent<br />

• 150 uL of sample<br />

• 50 uL of Ferric Reagent (samples only)<br />

• 50 uL Ammonia reagent<br />

• 1450 uL of distilled water (use 1500 uL for blank)<br />

After mixing and allowing a reaction time of 10 minutes, the absorbance<br />

was measured against a reagent blank at 600 nm wavelength in a<br />

spectrophotometer. Final result is calculated by multiplying corrected<br />

absorbance at 600 nm x 547 x dilution factor to give results in mg/L.<br />

Processing plant trials: A total of 185 tonnes of fruit thinnings were<br />

collected over a period of 5-7 weeks from mid November until the end of<br />

December. These were collected into waist belt held picking bags, before<br />

consolidation into 0.4 tonne holding bins. The bins of fruitlets were hydro-cooled,<br />

then stored in cool store at 4 degrees C until delivery to the processing plant on<br />

the day of processing.<br />

Fruit was processed on 6 Dec, 13 Dec, 21 Dec and 8-10 January. Milling<br />

occurred using a Rietz mill (10 mm screen) with addition of sodium<br />

metabisulphite solution at milling to give a level of approximately 300 mg/kg<br />

SO2. Some additional water was also added at milling to facilitate pumping to<br />

the mash holding tanks. Pectolytic mash enzyme was added at a rate of<br />

approximately 300 mL/Tonne, before holding for 1 – 2 hours at 20 – 25 degrees<br />

C. Juice was extracted using a Bucher HPX5005i fruit press, then flash<br />

pasteurised into agitated fining tanks where fining agents silica sol and bentonite<br />

were applied. The juice was then cross-flow filtered in a Bucher “Ministar –metal”<br />

unit employing Graver technologies filter elements having 0.1 micron pore size<br />

titanium oxide membranes supported on 316 sintered stainless steel. Finally, the<br />

juice was concentrated in an APV rising / falling film plate evaporator to around

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