Ionix Pharmaceuticals - Molecular Devices

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Assay Validation Calcium channel blockers were used to validate the assay Nimodipine -Conotoxin-GVIA Response (% Max) 100 75 50 25 0 Response (% Max) 100 75 50 25 0 0.0001 0.001 0.01 0.1 1 10 100 1000 Nimodipine concentration (M) N-Type IC 50 = 5.3 M L-Type IC 50 = 13 nM 1 10 100 -Conotoxin-GVIA concentration (nM) N-type IC 50 = 5.5 nM L-type No Block 16
Calcium Channel Screen 50,000 compound library screened 40 plates/day on FLEXstation Data analyzed with SOFTmax Pro 17
Page 1 and 2: Using the FLEXstation to investigat
Page 3 and 4: Ionix Profile Founded July 2001, C
Page 5 and 6: Voltage-gated Calcium Channels IMR
Page 7 and 8: IMR32 Cell Culture Conditions Cell
Page 9 and 10: Fura-2 Fluorescent Indicator 340/3
Page 11 and 12: KCl-Induced Calcium Changes In IMR-
Page 13 and 14: 1M Nicardipine +1M -CnTxGVIA 13 Pha
Page 15: Increasing CaCl 2 Concentration in
Page 19 and 20: 19 SOFTmax PRO Data Analysis
Page 21 and 22: Summary Developed assay to analyze
Page 23 and 24: Experimental Conditions Neuroblast
Page 25 and 26: Assay Validation Responses measure
Page 27 and 28: Summary of Data Cell Line SH-SY-5Y
Page 29 and 30: Summary Tetrodotoxin sensitive vol
Page 31: Acknowledgements Molecular Pharmac

Ionix Pharmaceuticals - Molecular Devices

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