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Ionix Pharmaceuticals - Molecular Devices

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Assay Validation<br />

Responses measured in BE(2)C cells<br />

Veratridine<br />

Tetrodotoxin<br />

(100M Veratridine)<br />

100000<br />

100000<br />

Response (RFU)<br />

75000<br />

50000<br />

25000<br />

Response (RFU)<br />

75000<br />

50000<br />

25000<br />

0<br />

1 10 100 1000<br />

0<br />

0.01 0.1 1 10 100 1000 10000<br />

Veratridine concentration (M)<br />

EC 50 = 13 M<br />

Tetrodotoxin concentration (nM)<br />

IC 50 = 6 nM<br />

25

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