Ionix Pharmaceuticals - Molecular Devices

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Adherent vs Suspension Assay Cells used in suspension IMR32 cells adhere to surfaces weakly Changing media every 2-3 days causes large loss of cells when grown in 96 well plates Cells grown in tissue culture flasks (under differentiating conditions, if necessary) and then loaded with fluorescent indicator and assayed in suspension 8
Fura-2 Fluorescent Indicator 340/380 ratio Disadvantage: It cannot easily be used with a FLIPR Fluo-4 was used in screening assay Can be used in FLIPR and hence in highthroughput assays Data obtained do not require ratio calculations. Therefore are easily manipulated 9
Page 1 and 2: Using the FLEXstation to investigat
Page 3 and 4: Ionix Profile Founded July 2001, C
Page 5 and 6: Voltage-gated Calcium Channels IMR
Page 7: IMR32 Cell Culture Conditions Cell
Page 11 and 12: KCl-Induced Calcium Changes In IMR-
Page 13 and 14: 1M Nicardipine +1M -CnTxGVIA 13 Pha
Page 15 and 16: Increasing CaCl 2 Concentration in
Page 17 and 18: Calcium Channel Screen 50,000 comp
Page 19 and 20: 19 SOFTmax PRO Data Analysis
Page 21 and 22: Summary Developed assay to analyze
Page 23 and 24: Experimental Conditions Neuroblast
Page 25 and 26: Assay Validation Responses measure
Page 27 and 28: Summary of Data Cell Line SH-SY-5Y
Page 29 and 30: Summary Tetrodotoxin sensitive vol
Page 31: Acknowledgements Molecular Pharmac

Ionix Pharmaceuticals - Molecular Devices

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