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Ionix Pharmaceuticals - Molecular Devices

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IMR32 Cell Culture Conditions<br />

Cells grown in EMEM supplemented with<br />

2mM Glutamine+1%NEAA+10%FBS<br />

Cells were split 1:2/1:3 every 2-3 days<br />

or had the media changed<br />

Differentiate with 1mM dibutyryl cAMP<br />

and 2.5µM bromodeoxyuridine for 8-10<br />

days<br />

7

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