datasheet - Dolomite Microfluidics
datasheet - Dolomite Microfluidics
datasheet - Dolomite Microfluidics
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The <strong>Dolomite</strong> Centre Ltd<br />
Unit 1, Anglian Business Park, Orchard Road,<br />
Royston, Hertfordshire, SG8 5TW, UK<br />
T: +44 (0)1763 242491<br />
F: +44 (0)1763 246125<br />
E: sales@dolomite-microfluidics.com<br />
W: www.dolomite-microfluidics.com<br />
Wellplate Chip<br />
Part No. 3000218<br />
<strong>Dolomite</strong> <strong>Microfluidics</strong> – North America Office<br />
Blacktrace Inc, 29 Albion Place<br />
Charlestown, MA 02129, USA<br />
C: 617 803 6655<br />
T: 617 848 1121<br />
F: 617 500 0136<br />
E: salesus@dolomite-microfluidics.com<br />
product <strong>datasheet</strong><br />
page<br />
Description 2<br />
Benefits 2<br />
Specifications 3<br />
Wellplate Chip Cross-sectional Profile 3<br />
Custom Well Geometry 4<br />
Custom Surface Coatings 5<br />
Wellplate Geometry 5<br />
Optical Transmission 5<br />
The <strong>Dolomite</strong> Centre Limited Page 1 of 6
The <strong>Dolomite</strong> Centre Ltd<br />
Description<br />
The Wellplate Chip is a glass chip with a 40 x 40 array of nano-litre wells. On the standard<br />
plate each well has a volume of 0.26nl, and customisation offers alternative volumes.<br />
The 40 x 40 array covers an area of 10 x 10mm and is designed to fit under a standard<br />
cover slip or in the resealable chip holder. The wet etching process gives a surface<br />
roughness of < 5nm enabling optical access.<br />
Applications include: holding small droplets of reagents or groups of cells or reagent<br />
droplets and display technology.<br />
Left: The Wellplate Chip (Part No. 3000218)<br />
Right: An image of the well matrix captured<br />
with a microscope.<br />
Below: The Resealable Chip Interface which<br />
can be used to flow fluids over the Wellplate<br />
Benefits<br />
• Enables experiments with nanoscale volumes<br />
• Fits onto a microscope slide and under a standard cover slip<br />
• Compatible with Mitos resealable chip holder<br />
• Excellent optical transmission<br />
• Custom sizes available<br />
• Smooth surfaces<br />
Wellplate Chip_product <strong>datasheet</strong> Page 2 of 6
The <strong>Dolomite</strong> Centre Ltd<br />
Specifications<br />
Chip Specification<br />
Value<br />
Chip Dimensions (Length x<br />
Width x Depth)<br />
29.8 x 22.3 x 2mm<br />
Array Size<br />
40 x 40 wells<br />
Array Area<br />
Well Dimensions (Diameter x<br />
Depth)<br />
Well Volume<br />
10 x 10mm<br />
105 x 50µm<br />
Note: Wells are isotropically etched hemispheres (see<br />
below)<br />
0.26nl<br />
Well Spacing<br />
250µm (centre to centre)<br />
Material<br />
Glass<br />
Surface Roughness<br />
5nm<br />
Wellplate Chip Cross-sectional Profile<br />
250µm<br />
105µm<br />
50µm<br />
2mm (Not<br />
to scale)<br />
A 2D cross-sectional sketch of the Wellplate Chip (left) and a 3D CAD image showing<br />
(right).<br />
Wellplate Chip_product <strong>datasheet</strong> Page 3 of 6
The <strong>Dolomite</strong> Centre Ltd<br />
Custom Well Geometry<br />
Wells can be etched to a range of depths or diameters. The well diameter is two times its<br />
depth . An example of a possible well geometry is 150µm deep with a diameter of 305µm.<br />
A 3D CAD image of<br />
wells that have been<br />
etched to a depth of<br />
150µm (diameter =<br />
305µm).<br />
Using two layers, more complex geometry can be formed.<br />
50 to<br />
100µm<br />
Custom Surface Coatings<br />
The surface of the glass chip is naturally hydrophilic. A hydrophobic coating is available.<br />
Other custom coatings such as gold or platinum can be applied to the well surface. Please<br />
contact <strong>Dolomite</strong> with any questions.<br />
An example of<br />
a coating<br />
applied to the<br />
well surface.<br />
Wellplate Chip_product <strong>datasheet</strong> Page 4 of 6
The <strong>Dolomite</strong> Centre Ltd<br />
Wellplate Geometry<br />
Optical Transmission<br />
Spectral Transmittance of 2mm glass layer<br />
100<br />
Transmittance, %<br />
80<br />
60<br />
40<br />
20<br />
0<br />
250 350 450 550 650 750<br />
Wavelength, nm<br />
If lower wavelengths are required fused silica (quartz) can also be used.<br />
Wellplate Chip_product <strong>datasheet</strong> Page 5 of 6
The <strong>Dolomite</strong> Centre Ltd<br />
Wellplate Chip_product <strong>datasheet</strong> Page 6 of 6
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