23.11.2014 Views

Bacteriophage application for elimination of E coli for elimination of ...

Bacteriophage application for elimination of E coli for elimination of ...

Bacteriophage application for elimination of E coli for elimination of ...

SHOW MORE
SHOW LESS

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Further characterization <strong>of</strong> phages<br />

• 20/42 phage isolates were considered highly lytic to<br />

E. <strong>coli</strong> O157:H7<br />

• Multiplicity <strong>of</strong> Infection (MOI) is unique <strong>for</strong> each phage<br />

• Contact t concentration ti <strong>of</strong> phages with target t is critical<br />

• Allows us to target low numbers <strong>of</strong> E. <strong>coli</strong> O157:H7<br />

found in meat (highly lytic)<br />

• Pulsed Field Gel Electrophoresis (PFGE) (genome<br />

sizing, restriction patterns) to type naturally-occurring<br />

phage and E. <strong>coli</strong> O157:H7 (SS and CTRL animals).

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!