EPR Spectroscopy

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Electron S (½) Nucleus I (1) Hyperfine Coupling M S =±½ M S = ±½ M S =+½ M I =0,±1 M I =+1 M I = 0 M I =-1 S=½; I=1 Triplet hfc hfc M S =-½ M I =-1 M I = 0 M I =+1 Selection Rule ∆M S = ±1; ∆M I = 0 Magnetic Field
What do we do with <strong>EPR</strong>? We can detect & measure free radicals and paramagnetic species • High sensitivity (nanomolar concentrations) • No background • Definitive & quantitative Direct detection e.g.: semiquinones, nitroxides, trityls Indirect detection Spin-trapping Species: superoxide, hydroxyl, alkyl, NO Spin-traps: DMPO, PBN, DEPMPO, Fe-DTCs Chemical modifications Spin-formation : hydroxylamines (Dikhalov et al) Spin-change : nitronylnitroxides ( Kalyanaraman et al) Spin-loss : trityl radicals
Page 1 and 2: EPR Spectroscopy Spying on unpaired
Page 3: Electron S(½) Nucleus I (½) Hyper
Page 7 and 8: What else can we do with EPR? We ca
Page 9 and 10: Can we image free radicals in biolo
Page 11 and 12: EPRI is capable of measuring the di
Page 13 and 14: Gradient Magnetic Field Homogeneous
Page 15 and 16: Projection s p(θ) Projectio n y s
Page 17 and 18: Image Reconstruction by Backproject
Page 19 and 20: 3D Image of a rat heart perfused wi
Page 21 and 22: IMAGING OF RAT KIDNEY PERFUSED WITH
Page 23 and 24: Molecular oxygen is paramagnetic 2p
Page 25 and 26: Principle of EPR oximetry R Ö 2 Ö
Page 27 and 28: EPR Oxegen Mapping (EPROM) [PDT, mM
Page 29 and 30: LiPc (Lithium Phthalocyanine) Oxyge
Page 31 and 32: Oxygen Measurements using LiPc, a P
Page 33 and 34: Redox Status Redox State describe t
Page 35 and 36: Reduction of 3-CP in the Normal & T
Page 37 and 38: REDOX MAPPING BY EPR IMAGING (VALID
Page 39 and 40: GSH levels in leg muscle (Normal) a
Page 41 and 42: Redox mapping of tumor: Effect of C
Page 43 and 44: Summary 1. EPR spectroscopy is a di
Page 45: determination and reversible modifi

EPR Spectroscopy

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