tumor cell biology program - Sylvester Comprehensive Cancer Center

Jiang, Z, Podack, ER and Levy, RB.
Donor T cells which cannot mediate
perforin dependent and FasL-dependent
cytotoxicity can effect graft versus host
reactivity following allogeneic bone
marrow transplantation. Periodicum
Biologorum 100:477, 1999.
Petito, CK, Kerza-Kwiatecki, AP,
Gendelman, HE, McCarthy, M, Nath,
A, Podack, ER, Shapshak, P and Wiley,
CA. Neuronal injury in HIV infection.
Journal of Neurovirology 5:327, 1999.
Prpic, L, Strbo, N, Sotosek, V,
Gruber, F, Podack, ER and Rukavina, D.
Assessment of perforin expression in peripheral
blood lymphocytes in psoriatic
patients during exacerbation of disease.
Acta Dermato-Venereologica. Supplementum
211:14, 2000.
Muta, H, Boise, LH, Fang, L and
Podack, ER. CD30 signals integrate expression
of cytotoxic effector molecules,
lymphocyte trafficking signals, and signals
for proliferation and apoptosis. Journal
of Immunology 165:5105, 2000.
Rukavina, D and Podack, ER.
Abundant perforin expression at the
maternal-fetal interface: guarding the
semiallogeneic transplant. Immunology
Today 21:160, 2000.
Jiang, Z, Podack, ER, Levy, RB.
Major histocompatibility complex-mismatched
allogeneic bone marrow transplantation
using perforin and/or Fasligand
double-defective CD4(+) donor
T cells: involvement of cytotoxic function
by donor lymphocytes prior to graftversus-host
disease pathogenesis. Blood
98:390, 2001.
Ohshima, K, Kawasaki, C, Muta, H,
Muta, K, Deyev, V, Haraoka, S,
Suzumiya, J, Podack, ER and Kikuchi,
M. CD10 and Bcl10 expression in diffuse
large B-cell lymphoma: CD10 is a
marker of improved prognosis. Histopathology
39:156, 2001.
Ohshima, K, Muta, H, Kawasaki, C,
Muta, K, Deyev, V, Kanda, M, Kumano,
Y, Podack, ER and Kikuchi, M. Bcl10
expression, rearrangement and mutation
in MALT lymphoma: Correlation with
expression of nuclear factor-kappa B.
International Journal of Oncology
19:283, 2001.
Hnatyszyn, H, Podack, ER, Young,
AK, Seivright, R, Spruill, G and Kraus,
G. The use of real-time PCR and
fluorogenic probes for rapid and accurate
genotyping of newborn mice. Molecular
Cell Probes 15:169, 2001.
HIGHLIGHTS/DISCOVERIES
• Heatshock fusion vaccines generate
CD8 CTL without CD4 help: progress
towards novel and efficient tumor specific
vaccines.
• CD30 is identified as a major negative
regulator of cytotoxic lymphocytes:
blocking CD30 signals in vivo will dramatically
enhance anti-tumor immune
responses.
• Generation of the first murine gene
knock in Florida: CD30-Ligand
knock-out in mice will serve as a valuable
model for tumor and autoimmunity
studies.
• Innovative vaccine trial for lung adenocarcinoma
approved by FDA.
• A role for perforin in lymphocyte homeostasis
revealed: cytotoxicity by
perforin is necessary to remove antigen
presenting cells and turn off T cell
activation.
Richard L. Riley, Ph.D.
Professor of Microbiology
and Immunology
DESCRIPTION OF RESEARCH
Altered B Cell Development in
Senescence
Senescent mice show diminished B lymphopoiesis
when compared to young
mice and typically exhibit decreased
numbers of pre-B cells in the bone marrow.
Dr. Riley’s laboratory has shown that
the molecules, λ5 and VrpeB, which
comprise the surrogate light chain component
of the pre-B cell receptor, are reduced
in pro-B/early pre-B cells both ex
vivo and when derived in vitro from the
bone marrow of 18-27-month-old
BALB/c mice after stimulation with IL-
7. Both λ5 and VpreB expression were
decreased at the mRNA level as indicated
by semi-quantitative RT-PCR; this suggests
that the reduced surrogate light
chains seen in senescent B cell precursors
result from dysfunctional transcriptional
regulation. The transcription of
surrogate light chains is principally regulated
by E2A (E47) and Early B cell Factor
(EBF) gene products. EBF mRNA
levels were estimated to be only slightly
reduced in cultured senescent pro-B/
early pre-B cells (