Three New Species of Gregarines (Apicomplexa: Sporozoea ...

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304 P.K. Prasadan and K.P. Janardanan Types of all parasites (stained specimens on slides) and their host crabs are deposited in the parasite collections, Parasitology Laboratory, Department of Zoology, University of Calicut, Kerala (India). RESULTS Nematopsis messor sp. n. (Fig. 1, Table 1) Description Sporadins (Fig.1.1): biassociative; association caudofrontal; colour milky-white. Syzygy early, linear. Primites: protomerite shape hemispherical, wider than long, maximum width at posterior end; transparent lensshaped structure present at anterior end; epicyte uniformly thick, striated; endocyte granular. Septum circular, convex toward deutomerite; constriction at septum inconspicuous. Deutomerite ovoid, narrow behind septum, gradually widens caudally, posterior end broadly rounded; maximum width in posterior half; epicyte uniformly thick; endocyte granular. Nucleus spherical, faintly visible in fresh sporadins, variable in position; endosome single, spherical. Satellites: lengths on average slightly shorter than primites. Protomerite shape hemispherical, wider than long; epicyte uniformly thick, striated; endocyte granular with transparent lens-shaped structure at anterior end. Septum similar to primite (see above). Deutomerite ovoid, narrow behind septum, gradually widens caudally, posterior end broadly rounded; maximum width at posterior half. Nucleus and endosome similar to primite (see above). Gametocysts (Fig. 1.2): shape spherical; colour milkywhite. Cyst wall double; epicyst thick, hyaline, width uneven, 6.6-11.5; endocyst thin, width uniformly even, 1.6. Gymnospores (Fig. 1.3): shape spherical; diameter 4.5-5.3; uninucleated bodies arranged radially in rosette pattern around central, hyaline cytoplasm. Trophozoites (Figs 1.4-1.6): development extracellular. Smallest observed trophozoite (Fig. 1.4) has a hemispherical protomerite and ovoid deutomerite. In an association measuring 129.6 long (Fig. 1.6), the primite measured 70.7 by 37.1 and satellite 58.6 by 37.1. Taxonomic summary Type specimens: syntypes, No.Z/Par/G/101; deposited in the parasite collections, Parasitology Laboratory, Department of Zoology, University of Calicut, Kerala, India. Type host: Metapograpsus messor (Forskäl) (Arthropoda: Crustacea: Grapsidae). Symbiotype: host crab preserved in the Parasitology Laboratory (see address above). Additional hosts: None. Type locality: India, Kerala, Malppuram district, Kadalundi Estuary, sandy and rocky intertidal. Additional localities in India: Kannur district, Mavilayi and Muzhappilangad; Kozhikode district, Iringal and Kizhariyur (all sandy and rocky river banks). Collection dates: 1988 (February, March, November, December); 1989 (October through December); 1990 (January through April). Site of infection: intestine. Prevalence: in 8 of 22 crab hosts examined. Etymology: named after the species of crab host. Remarks Nematopsis messor sp. n. resembles most to N. raoudi Vivárès, 1971 from the crab Portunus latipes. However, it differs in several significant ways, including the shape of primites and satellites; and in the presence of (1) a prominent lens-shaped structure at the anterior part of the protomerite, (2) a spherical nucleus and (3) a protomerite in the satellites. In addition, the species described above is from a different host and a different geographical locality. This is the first report of gregarines from Metapograpsus messor (Forskäl). Nematopsis quadratum sp. n. (Fig. 2, Table 1) Description Sporadins (Fig. 2.1): biassociative, association caudofrontal; colour milky-white. Syzygy early, linear. Primites: always larger than satellites. Protomerite shape hemispherical with a plano-convex to biconvex lens-shaped structure at anterior part; epicyte uniformly thick, striated; endocyte granular. Septum circular, convex toward deutomerite. Deutomerite elongate ovoid, narrow behind septum gradually widens caudally, posterior end broadly rounded; maximum width in posterior half; epicyte hyaline; endocyte granular; a transparent lens-shaped structure at posterior part. Interlocking mechanism between primite and satellite well developed. Nucleus spherical to ovoid. Satellites: protomerite and septum similar to that of primite (see above). Deutomerite widest behind septum, gradually tapers caudally, posterior end rounded. Nucleus
Gregarine new species 305 Figs 1.1-1.6. Nematopsis messor sp. n.; 1.1 - sporadins; 1.2 - gametocyst; 1.3 - gymnospore; 1.4 - early trophozoites; 1.5, 1.6 - early associations. Scale bars - 1.1, 1.2, 1.6 - 50 µm; 1.4, 1.5 - 25 µm ; 1.3 - 10 µm spherical to ovoid, faintly visible in fresh sporadins, variable in position; endosome single, round to ovoid. Gametocysts (Fig. 2.2): shape spherical; colour milkywhite, opaque. Cyst wall double; epicyst thick, hyaline, width uneven, 20.4 - 40. Gymnospores (Fig. 2.3): shape spherical; diameter 7.5; uninucleated bodies arranged radially in rosette pattern around central, hyaline cytoplasm. Trophozoites: development extracellular. Smallest observed trophozoite (Fig. 2.4) has a hemispherical, protomerite and cylindrical deutomerite. Largest observed trophozoite was elongate, almost cylindrical, with hemispherical protomerite and elongated deutomerite. Taxonomic summary Type specimens: syntypes; No.Z/Par/G/102; deposited in the parasite collections, Parasitology Laboratory, Department of Zoology, University of Calicut, Kerala, India. Type host: Sesarma quadratum (Fabricius) (Arthropoda: Crustacea: Grapsidae). Symbiotype: host crab preserved in the Parasitology Laboratory (see address above). Additional host: none. Type locality: India, Kerala, Malappuram district, Olippuram rocky river banks. Additional localities in India: Kannur district, Mavilayi and Muzhappilangad; Kozhikode district, Feroke and Ramanattukara;
Page 1: Acta Protozool. (2001) 40: 303 - 30
Page 5 and 6: Gregarine new species 307 Figs 3.1-
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