Cryobiology and cryopreservation of human gametes and ... - eshre

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General outlook to cryopreservation by slow freezing and vitrification Slow freezing Vitrification CPA toxicity + + + + Osmotic stress + + + + + Solution effect toxicity + + + - Intracellular ice risk + + - Accidental thawing/devitrification risk - + + Technology dependence + + + + Costs + + + + + + Operational times + + + + Contamination risk - + +
Technology Robust Operator friendly Reproducible (well established in IVF labs for decades) Small variability Intra-operator Intra-laboratory Inter-laboratory Allows monitoring of cooling process for quality assurance 40 20 0 -20 -40 -60 -80 -100 -120 -140 -160 0 15 30 45 60 75 90 105 120
Page 1 and 2: ESHRE Campus Symposium Cryobiology
Page 3: Cellular differences make oocytes o
Page 7 and 8: Margins of improvement … In the d
Page 9 and 10: Cell damage from cryopreservation P
Page 11 and 12: Clinical efficiency of oocyte cryop
Page 13: Spindle and chromosome dynamics in
Page 16 and 17: Impact of protocol Female age < 35
Page 18 and 19: “Activity of maturation promoting
Page 20 and 21: Efficiency: slow freezing vs. vitri
Page 22 and 23: Embryo vs. oocyte slow freezing Cum
Page 24: Conclusions: Controlled rate slow f

Cryobiology and cryopreservation of human gametes and ... - eshre

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