Introduction to NGS technologies - Bioinformatics and Genomics ...

More documents

Recommendations

Info

Information Bioinformatics tools for pre-genomic sequence data analysis Molecular databases Search results Phylogenetic tree Sequence Motif databases Motif alignment Conserved region Secondary and tertiary protein structure
Page 1 and 2: Introduction to NGS Technologies Ja
Page 3: Genetic Research …code for protei
Page 7 and 8: High Throughput Technologies • 19
Page 9 and 10: Next Generation Sequencing SOLID 12
Page 11 and 12: Date Cost per Mb Cost per Genome Da
Page 13 and 14: Pre & Post-genomic databases EMBL d
Page 15: The size of the human genome is ~ 3
Page 18 and 19: Relative throughput of the differen
Page 20 and 21: Basics of the “new” Technology
Page 22 and 23: NGS technologies Cost-effective Fas
Page 24 and 25: Similarities- LOTS of DATA General
Page 26 and 27: Next-gen sequencers From John McPhe
Page 28 and 29: 454 (Roche) -First next generation
Page 30 and 31: Roche 454 pyrosequencing
Page 32 and 33: Roche 454
Page 34 and 35: Solexa (Illumina) •Over 90% of al
Page 36 and 37: Solexa / illumina From Michael Metz
Page 38 and 39: Illumina-HiSeq 2500 600 Gb/run in 1
Page 40 and 41: •Colorspace SOLiD (ABI / Life Tec
Page 42 and 43: SOLiD * Sequencing output in “col
Page 45 and 46: SOLiD_Dibase_Sequencing_and_Color_S
Page 47: SOLiD color space SOLiD_Dibase_Sequ
Page 50 and 51: AB SOLiD: Dibase Sequencing AB SOLi
Page 52 and 53: AB SOLiD: Variations SOLiD_Dibase_S
Page 54 and 55:
SOLiD 5500 *Sixth 3-based encoded p
Page 56 and 57:
Pacific Bioscience SMRT: Singel Mol
Page 58 and 59:
Ion Proton
Page 60 and 61:
Comparison Roche 454 Illumina SOLiD
Page 62 and 63:
Basic steps NGS data processing QC
Page 64 and 65:
Basic steps NGS data processing QC
Page 66 and 67:
Most common applications of NGS RNA
Page 68 and 69:
DNA sequencing - 2 • Whole GENOME
Page 70 and 71:
• Targeted Resequencing - Capture
Page 72 and 73:
Transcriptomics - 1 • RNA-Seq - S
Page 74 and 75:
Transcriptomics - 2 • SAGE. Seria
Page 76 and 77:
Applications of RNAseq Qualitative:
Page 78 and 79:
Transcriptomics - 3 • miRNA/small
Page 80 and 81:
Egigenomics - I Epigenomics refers
Page 82 and 83:
Egigenomics - 2 MeDIP-Seq, methylat
Page 84 and 85:
Census NGS methods
Page 88:
Miller syndrome
Page 92 and 93:
No so far…. Sequencing Centers
Page 94 and 95:
CASEGH
Page 96 and 97:
Two technologies to scan for variat
Page 98 and 99:
Bioinformatics Unit 24 High Perform
Page 100 and 101:
The Medical Genome Project (MGP) Pu
Page 102 and 103:
The MGP Scope and Aims • Medical
Page 104 and 105:
Normalized coverage Exome capture r
Page 106 and 107:
Exome sequencing work-flow for Vari
Page 108 and 109:
SNPs distribution across samples
Page 110 and 111:
Secondary analysis: Finding the mut
Page 112 and 113:
The principle: comparison of patien
Page 114 and 115:
Is this approach realistic? Can we
Page 116 and 117:
Clear individual gene associations
Page 118 and 119:
NGS is revolutionizing how we do ge
Page 120:
THANK YOU
show all

Introduction to NGS technologies - Bioinformatics and Genomics ...

Create successful ePaper yourself

Delete template?

Save as template?