Gel Protein Separation & Recovery - Protea Biosciences

Gel 

Protein 

Separation 

& 

Recovery 

Advanced Gel Protein Separation and Recovery Technology 

Gel Protein Separation & Recovery 

protea

Gel Protein Recovery ® (GPR) 

Protea’s Gel Protein Recovery System (GPR) is an advanced, 

microfluidics-based system for the rapid and efficient extraction 

of proteins from polyacrylamide gels. 

The GPR-800 system uses a proprietary plastic microfluidic 

chip in a high voltage environment, to allow fast electroelutions 

simultaneously in eight parallel microchannels. The GPR 

Electroelution Buffers contains a proprietary mass spec friendly 

surfactant that allows for easy degradation and clean-up prior to 

mass spectrometry. Protea’s GPR-800 is a closed system with 

minimal dead volume, assuring no introduction of contaminants. 

FEATURES: 

Advanced Microfluidic Chip Design – Allows for 

efficient extraction of proteins down to 100 ng. Intact mass 

measurements are routinely achieved from coomassie stained 

gels. Limited surface area greatly improves recovery rate. 

Eight Channel Parallel Processing – 8 gel bands can be 

processed simultaneously in less than 20 minutes. More than 100 

proteins can be recovered in a standard work day. 

Proprietary Decoupled Protein Recovery – Allows 

for easy and efficient collection of protein samples for post 

electroelution clean-up. 

System and Reagents Designed for Mass 

Spectrometry – Proteins can be mass spectrometry ready 

without extensive clean-up. 

Closed System and Disposable Chips – No worries about 

contamination or carry over. Use a new, clean chip for each 

recovery. 

RESEARCH APPLICATIONS: 

Top-Down Proteomics – Provides the ability to combine the 

resolution and quantitation of 2D electrophoresis with top-down 

proteomics using state of the art mass spectrometry techniques. 

Intact Mass Measurement – Quickly confirm protein mass 

from 1D and 2D gels using mass spectrometry. 

Determine Modification State of Proteins – Use the 

resolving power of 2D electrophoresis to resolve isoforms, 

degradation products, and modifications states. Quickly recover 

separated species for mass spectrometry analysis. 

Recover and Store – Proteins can rapidly be recovered from 

1D or 2D gels and stored for later experimentation. 

Effective Range of the GPR 

The plot shows the amount of protein needed (in gel) as 

a function of molecular weight for successful usage of 

the GPR-800. In general, higher protein loads and lower 

molecular weight proteins yield the best results. The blue 

shaded area represents the range of protein gel loads where 

it is routine to obtain an intact mass measurement after 

protein recovery. The area shaded in green represents the 

range where it is routine to successfully identify a recovered 

protein after digestion using the GPR-800. It should be 

noted these areas represent areas of routine success. In 

many cases the ranges can be extended. For example we 

can, in many cases, show intact mass measurements for 

small proteins with loads down to 100 ng. In addition, we 

have successfully performed post electroelution digestion on 

many larger proteins (75 kDa) down to 250 ng. 

www.proteabio.com • 2

Isoform Analysis using GPR-800 

1 

16997 

1571.0 

2544.4 

1.0E+4 

% Intensity 

17864 

18834 

% Intensity 

2 

17030 

17905 

18888 

% Intensity 

3 

16961 

17801 

18643 

2188.3 

% Intensity 

4 

16965 

17806 

The GPR-800 was used to recover 

protein isoforms of myoglobin that 

were separated by 2-dimensional 

gel electrophoresis. Intact mass 

measurements showed that each 

resolved spot had statistically 

different masses. This approach 

allows researchers to easily 

quantitate and separate protein 

isoforms using gel electrophoresis 

followed by the rapid and efficient 

recovery of the intact molecules 

from the gel. Top down mass 

spectrometric techniques can then 

be used to determine intact mass 

and sequence information for each 

protein isoform. 


19475 

18650 

10000 14000 18000 22000 26000 30000 

10000 14000 18000 22000 26000 30000 

10000 14000 18000 22000 26000 30000 

10000 14000 18000 22000 26000 30000 

Mass (m/z) 

Mass (m/z) 

Mass (m/z) 

Mass (m/z) 

Sensitivity Data for the GPR-800 by ESI 

100 

1 4 + 

A 

100 ng 

Relative Abundance 

0 

800 1000 1200 1400 1600 1800 

m /z 

100 

1 4 + 

50 


0 

800 1000 1200 1400 1600 1800 

m /z 

20+ 

100 

22+ 

B 

C 

500 ng 

1 μg 

The three panels show ESI mass 

spectra of myoglobin after gel 

recovery using the GPR-800. Each 

spectrum represents a different initial 

gel load from 1 μg down to 100 ng. 

Panel A shows a mass spectrum for 

a 100 ng protein load indicating that 

the system is capable of recovering 

and mass analyzing most Coomassie 

stained visible spots. 

18+ 

24+ 

16+ 

26+ 


14+ 

0 

600 800 1000 1200 1400 

m /z 

3 • 877.776.8321

GPR-800 Instruments and Bundles 

GPR-800 Gel Protein Recovery System 

Includes GPR-800 instrument with power cable, software for 

current monitoring, chip holder, vial rack, and manual 

Catalog Number Size Price 

GPR-800 ea. POR* 

*Price on Request 

GPR-800 Gel Protein Recovery System Bundle A 

Includes GPR-800 instrument with power cable, software for 

current monitoring, chip holder, electrode alignment tool, vial rack, 

manual, 1 Protein Recovery Reagent Bundle, and 2 GPR Mass 

Spec Application Kits 


GPR-800-A ea. POR* 

GPR-800 Reagent Bundles and Kits 

GPR LC-MS Top-Down Prep Kit, Low MW 

Contains Surfactant Degradation Reagent for ESI and C 8 

GPR 

SpinTip Kit for ESI 


GPR-050 ea. $152 

GPR MALDI Intact Mass Measurement 

Prep Kit, Low MW 

Contains Surfactant Degradation Reagent, and C 8 

GPR SpinTip 

Kit for MALDI, C 8 

LithTip Sample Prep Kit, and Ultrapure CHCA 

MALDI Matrix 


GPR-055 ea. $289 

GPR LC-MS Bottom-Up Prep Kit, Low MW 

Contains Surfactant Degradation Reagent for ESI, and C 8 

GPR 

SpinTip Kit for ESI, and a Trypsin Digestion Kit 


GPR-060 ea. $257 

GPR MALDI Bottom-Up Prep Kit, Low MW 

Contains Surfactant Degradation Reagent and C 8 

GPR SpinTip Kit 

for MALDI, C 18 

LithTip Sample Prep Kit, Ultrapure CHCA MALDI 

Matrix, and a Trypsin Digestion Kit 


GPR-065 ea. $394 

GPR Centrifugal UF Sample Prep Kit, 10 kDa MWCO 

GPR Centrifugal Ultrafiltration Sample Prep Kit, 10kDa MWCO: 

Contains Surfactant Degradation Reagent for ESI, Wash Solution 

A, Wash Solution B, and 0.5mL Centrifugal Filter Units, 10 kDa 

MWCO 


GPR-068 ea. $184 

GPR-800 Protein Recovery Reagent Bundle 

Contains 10 Protein GPR chips, two GPR Electroelution buffers, 

gel spot picker and foreceps 


GPR-070 ea. $730 

GPR LithColumn LC-MALDI Kit, 100 µm ID 

Contains Surfactant Degradation Reagent, 100 µm C 8 

LithColumn, 

and Ultrapure CHCA MALDI Matrix 


GPR-080 ea. $788 

GPR LithColumn LC-MALDI Kit, 250 µm ID 

Contains Surfactant Degradation Reagent, 250 µm C 8 

LithColumn, 

and Ultrapure CHCA MALDI Matrix 


GPR-081 ea. $788 

GPR Centrifugal UF Sample Prep Kit, 3 kDa MWCO 

GPR Centrifugal Ultrafiltration Sample Prep Kit, 3 kDa MWCO: 

Contains Surfactant Degradation Reagent for ESI, Wash Solution 

A, Wash Solution B, and 0.5mL Centrifugal Filter Units, 3 kDa 

MWCO 


GPR-067 ea. $184 

For Additional Information: 

Razunguzwa, T.T., Biddle, A., Anderson, H., Zhan, D., Powell, M.J. Development of a microfluidics-based gel protein recovery 

system. Electrophoresis, 2009, 30, 4020-4028. 

Li, M., Powell, M.J., Razunguzwa, T.T., O’Doherty, G.A. A general approach to anionic acid-labile surfactants with tunable 

properties. J. Org. Chem. 2010, 75, 6149–6153. 


GPR-800 Buffers 

GPR Electroelution Buffer 


GPR-020-25mL 25 mL $100 

GPR-020-4x25mL 4 x 25 mL $310 

GPR Electroelution Buffer II, Acetate 


GPR-021-25mL 25 mL $100 

GPR-021-4x25mL 4 x 25 mL $310 

GPR Electroelution Buffer III, CAPS 


GPR-022-25mL 25 mL $100 

GPR-022-4x25mL 4 x 25 mL $310 

GPR Electroelution Buffer IV, Tris-Glycine Plus 


GPR-023-25mL 25 mL $131 

GPR-023-4x25mL 4 x 25 mL $415 

GPR Electroelution Buffer V, CAPS Plus 


GPR-024-25mL 25 mL $131 

GPR-024-4x25mL 4 x 25 mL $415 

Surfactant Degradation Reagent 


GPR-025-10mL 10 mL $47 

Surfactant Degradation Reagent for ESI 


GPR-026-10mL 10 mL $47 

Gel Spot Picker 


GPR-045-1 1 unit $152 


GPR-800 Protein GPRchips 

GPR-800 Warranty 

Protein GPRchip 

Contains 8 channels for protein electroelution from polyacrylamide 

gel samples 


GPR-200-10 10 units $473 

GPR-200-25 25 units $1050 

GPR 1 Year Extended Warranty 

Extends the GPR-800 warranty by 1 year 


GPR-900-1 1 unit $2,500 

GPR-800 Buffer Selection Guide 

Protein Characteristics Recommended GPR Buffer Part Number 

High and low pl hydrophilic proteins GPR Electroelution Buffer GPR-020 

Intermediate and high pl hydrophilic proteins 

Low and intermediate pl hydrophilic proteins 

High and low pl hydrophobic proteins and antibodies 

Low and intermediate pl hydrophobic proteins and 

antibodies 

GPR Electroelution Buffer II, 

Acetate 

GPR Electroelution Buffer III, 

CAPS 

GPR Electroelution Buffer IV, 

Tris-Glycine Plus 

GPR Electroelution Buffer V, 

CAPS Plus 

GPR-021 

GPR-022 

GPR-023 

GPR-024 

5 • 877.776.8321

ProteaGel Mini Precast Gels 

The ProteaGel Mini Precast Gels have been designed to 

improve polyacrylamide gel electrophoresis (PAGE) for both 

1D and 2D protein separations. ProteaGel polyacrylamide gel 

formulations are featured in the mini precast gels, permitting 

high resolution protein gel separation across a broad molecular 

weight range. The BR series of ProteaGel Mini Precast Gels are 

compatible with the Bio-Rad Mini-PROTEAN ® , Mini-PROTEAN 

Tetra cell, and the Mini-PROTEAN 3 Dodeca cells, as well as, 

other standard mini gel systems. The IV series of ProteaGel 

Mini Precast gels are compatible with the Invitrogen XCell6 

MultiGel and XCell SureLock Mini electrophoresis systems. 

The ProteaGel Mini product line offers a variety of precast gels to 

meet the protein separation needs of Life Science researchers. 

Standard mini precast gels are available in 10 and 15 well formats 

that permit loading of 35 and 20 µL samples, respectively. The 

8.4 cm gel width is ideal for the second dimension polyacrylamide 

gel electrophoresis separation in 2D PAGE analyses, as standard 

length 7 cm IPG strips can be directly loaded onto an IPG + 1 

precast gel. Empty gel cassettes are also available for pouring of 

custom gel formulations in any lab. 

FEATURES: 

• Precast polyacrylamide ProteaGels for 1D and 2D protein 

gel separations 

• High resolution gels over broad molecular weight 

range (5 to 250 kDa) 

• Hybridized formulation for increased gel strength 

• 7.5, 10, 12, and 15% gel options available 

• Each 1D gel contains a 5% stacking gel to ensure 

accurate running of the gel. 

• Compatible with BioRad Mini-PROTEAN, Mini-PROTEAN 

Tetra cell, Mini-PROTEAN 3 Dodeca cells, and the 

Invitrogen XCell6 MultiGel and XCell SureLock Mini 

• Certified Mass Spec Grade 

• Multiple cassette options available (precast ProteaGels and 

empty cassettes) 

• 10 well (35 µL) 

• 15 well (20 µL) 

• IPG + 1 (for use with 7 cm IPG strips) 

1 2 3 4 5 6 7 8 9 10 

A 10% ProteaGel separation stained with SuperBlue Coomassie 

gel stain (SB-G250). Lanes 1 and 10: Protein MWM (MM-101). 

Lane 2: human serum sample depleted of albumin and IgG. 

Lane 3: human serum sample. Lanes 4, 5, and 6: standard 

proteins. Lane 7: mixture of standard proteins. Lanes 8 and 

9: total protein lysate from human tissue (brain and liver, 

respectively). 


• SDS-PAGE separation of proteins 

• Two-dimensional gel electrophoresis 

• Biomarker separation 

• Sample complexity analysis 

• Recombinant protein purity analysis 

SPECIFICATIONS: 

• Cassette dimensions: 10.1 cm x 8.6 cm x 4.52 mm (width x 

height x thickness) 

• Gel dimensions: 8.4 cm x 6.1 cm x 1 mm (width x height x 

thickness) 

• Gel matrix: ProteaGel polyacrylamide hybrid formulation 

• Compatibility: BioRad Mini-PROTEAN, Mini-PROTEAN 

Tetra cell, and the Mini-PROTEAN 3 Dodeca cells 

• Cassette construction: polyester 

• Shelf-life: At least 3 months for precast cassettes and 5 

years for empty cassettes 


Multiple comb options available 

Laser-etched wells for easy loading 

Clear plastic 

cassette 

Designed 

specifically for 

downstream mass 

spec applications 


ORDERING INFORMATION: 

Hybrid gel formulation for high strength 

and superior resolution 

Acrylamide % Catalog Number Well Format System Number of Gels Price 

BR-000-10 10 well BioRad 10 pack $55 


Empty BR-000-IPG IPG + 1 BioRad 10 pack $55 

IV-000-10 10 well Invitrogen 10 pack $65 


IV-000-IPG IPG + 1 Invitrogen 10 pack $65 



7.5% BR-075-IPG IPG + 1 BioRad 10 pack $85 






10% BR-010-IPG IPG + 1 BioRad 10 pack $85 
















7 • 877.776.8321

ProteaGels 5%, 10%, 12% and 15% Solutions 

ProteaGels “ready-to-run” SDS polyacrylamide solutions 

polymerize into an advanced molecular sieve for the 

electrophoretic separation of proteins. Because of the advanced 

buffer chemistry used in the gel matrix solution, ProteaGels allow 

a single separating gel. A stacking gel is not required, as the 

ProteaGel proprietary formulation inherently stacks the protein 

samples during the normal electrophoresis run. However, for the 

highest resolution a stacking gel usng 5% ProteaGel solution can 

be used. Band resolution is unparalleled over a molecular range 

of 2.5 to 250 kDa. The new hybrid formulation of ProteaGels 

gives these gels an increased gel strength, which allows for 

easier handling. ProteaGels will work with all types of universal 

electrophoresis apparatus. Our gel mixtures are formulated for 

optimal performance in mass spectrometry-based proteomics 

experiments. 

FEATURES: 

• High gel strength – allows easier handling 

• Ready to use in less than 10 minutes – just add TEMED and 

APS to polymerize the gel 

• No stacking gel required – permits longer gel separations 

• High resolution gels for protein separation across a broad 

molecular weight range 

• Stable at room temperature for 1 year 

• Each batch tested for MS compatibility – Certified MS Grade 


• SDS-PAGE separation of proteins 

• Biomarker separation 

• Recombinant protein purity analysis 


ProteaGel 5% Solution 


PG-401D-30mL 30 mL $16 

PG-401D-100mL 100 mL $37 

PG-401D-500mL 500 mL $131 



PG-402D-30mL 30 mL $16 

PG-402D-100mL 100 mL $37 

PG-402D-500mL 500 mL $131 

A 5% ProteaGel separation of a dilution series of standard 

proteins stained with Protea’s SuperBlue R-250 Coomassie 

stain. Lane 1 contains a molecular weight marker. Lanes 3 

to 6 contain a mixture of standard proteins (cytochrome C, 

myoglobin, carbonic anhydrase, and bovine serum albumin) with 

mass loads of 2.0, 1.0, 0.5, and 0.25 µg per protein, respectively. 



PG-403D-30mL 30 mL $16 

PG-403D-100mL 100 mL $37 

PG-403D-500mL 500 mL $131 



PG-404D-30mL 30 mL $16 

PG-404D-100mL 100 mL $37 

PG-404D-500mL 500 mL $131 


SuperBlue G250 and R250 Coomassie Stains 

Protea’s SuperBlue Coomassie stains are an excellent choice for 

visualizing proteins separated by SDS-PAGE gel electrophoresis. 

Our SuperBlue G250 stain is a colloidal Coomassie formulation 

with a sensitivity of ~20-40 ng protein. As a colloidal stain, the 

SuperBlue G250 reacts only with proteins and not the gel itself, 

resulting in a reduced background staining that permits rapid 

visualization of the gel bands. Protea’s SuperBlue R250 stain 

is a traditional Coomassie stain, with a sensitivity of ~20-40 ng. 

Because Coomassie stains interact stoichiometrically with protein 

in gels, they are useful for quantitation by densitometry. The 

SuperBlue G250 stain is available as a ready-to-use formulation, 

while the SuperBlue R250 stain is provided as a 10X concentrate 

solution. 

FEATURES: 

• Sensitive – as low as 20 - 40 ng/band 

• Non-fixative and fixative protocols available 

• Useful for quantitation by densitometry 


• Staining 1D and 2D SDS-PAGE gels 

• Protein studies requiring non-fixative gel staining 

• Densitometry studies for protein quantitation in SDS-PAGE 

gels 


SuperBlue Coomassie Stain, G250 


SB-G250-500mL 500 mL $42 

SB-G250-2L 2 L $152 

SuperBlue Coomassie Stain, R250 


SB-R250-50mL 50 mL $21 

SB-R250-250mL 250 mL $79 

kDa 

1 2 3 4 5 

200 

116 

97 

66 

55 

45 

36 

29 

24 

20 

14 

6.5 


proteins stained with Protea’s SuperBlue G250 Coomassie 

stain. Lane 1 contains Protea’s Protein Molecular Weight 

Markers (MM-101-1). Lanes 2 to 5 contain a mixture of 

standard proteins (low to high molecular weight: myoglobin, 

carbonic anhydrase, bovine serum albumin, phosphorylase b, 

and myosin) with mass loads of 0.125, 0.25, 0.5, and 1.0 μg 

per protein, respectively. 


Application SuperBlue G250 SuperBlue R250 Zinc Stain Silver Stain 

1D SDS PAGE X X X X 

2D SDS PAGE X X X X 

Compatible with mass 

spectrometry 

X X X X 

Compatible with intact 

protein recovery 

X X X Not Recommended 

Compatible with in-gel 

digestion 

X X X X 

Fixing Required X X 

Postitive Stain X X X 

Negative Stain 

X 

Staining Time 1 Hour 1 Hour 15 Minutes 35 Minutes 

Destaining Time 30 Minutes 2-5 Hours Not Required Not Required 

Sensitivity 20-40 ng 20-40 ng 1 ng 1 ng 

Catalog Numbers 

SB-G250-500 

SB-G250-2L 

SB-R250-50mL 

SB-R250-250mL 

ZS-100-100mL 

ZS-100-500mL 

SS-100-kit 

SS-101-kit 

9 • 877.776.8321

Silver Stain Kit 

Silver staining of proteins is a useful technique for the detection 

of minor protein components that are difficult to visualize by 

standard Coomassie blue stains. Silver stains are 50 to 100-fold 

more sensitive than Coomassie and thus capable of detecting 

nanogram quantities (~2 ng) of protein on gels in comparison to 

Coomassie stains (20 - 40 ng). 

kDa 

200 

116 

97 

1 2 3 4 

5 6 

The Protea Silver Stain formulation is unique in that the staining 

process can be completed in about 35 minutes (including the 15 

min. fixing step) without sacrificing the sensitivity of detection. In 

addition, the silver stain kit is compatible with MALDI analysis, 

especially when used in conjunction with our silver destaining 

reagent. 

FEATURES: 

• 35 minutes from fixation to visualization of protein bands 

• Stable at room temperature for up to 6 months 

• Sensitivity (1-2 ng) is 50 to 100x greater than that of 

Coomassie stain 

• Mass Spec tested and fully compatible (after silver 

destaining step) 

• Certified Mass Spec Grade 


• Biomarker discovery 

• Protein identification and characterization 

• Proteomics method development 

66 

55 

45 

36 

29 

24 

20 

14 


proteins stained with Protea’s Silver Stain. Lane 1 contains 

Protea’s protein molecular weight marker. Lanes 2 to 6 contain 

a mixture of standard proteins (myoglobin, ovalbumin, and 

bovine serum albumin) with mass loads of 250, 125, 62.5, 

31.25, and 15.625 ng per protein, respectively. 


Silver Stain Kit 

Certified MS Grade. Includes reagents for silver staining 

procedure, 4 x 250 mL 


SS-100-Kit 1 Kit $152 

Silver Stain Kit XL 

Certified MS Grade. Includes reagents for silver staining 

procedure, 4 x 1 L 


SS-101-Kit 1 Kit $504 

Silver Destaining Solution 

Silver Staining is a very powerful technique to detect 

small amounts protein after one- or two-dimensional gel 

electrophoresis. Unfortunately, the silver deposits on the protein 

tend to interfere with mass spectrometry analysis, and thus its 

precise identification. Protea’s Silver Destaining Solution is readyto-use 

and removes most if not all of the silver from proteins and 

permits mass spectrometry analysis. 


Silver Destaining Solution 


SS-110-250mL 250 mL $37 


Zinc Stain 

ZincPlus Stain Kits reagents are used to stain one- or twodimensional 

gels following SDS-PAGE. The ZincPlus Stain is a 

negative stain, which produces a whitish opaque background in 

the gel, while the protein bands themselves remain unstained. 

The inherent benefit of a negative stain is that the protein band 

does not contain any dye or stain to present as a contaminant for 

downstream sample processing and analysis. ZincPlus Stain Kit is 

a fast and reversible method for visualizing proteins separated on 

polyacrylamide gels. The ZincPlus Stain has a detection limit of ~1 

ng of protein, and the entire staining process is completed within 

15 minutes. Unlike most stains, ZincPlus Stain does not require a 

fixation step, which leaves the proteins unaffected for downstream 

processing. 

FEATURES: 

• Negative stain: no dye or stain contaminating the protein bands 

• Fast staining technique:

TriColor Pre-stained Protein Molecular Weight Markers 

Protea’s Blue and TriColor Pre-stained Protein Molecular Weight 

Markers are designed for use with SDS-PAGE gels. They cover 

a wide range of molecular weights from 7 to 175 kDa. Blue 

Pre-stained Protein Molecular Weight Markers are comprised 

of 8 proteins covalently coupled to a blue dye, allowing easy 

visualization during electrophoresis. TriColor Pre-stained Protein 

Molecular Weight Markers are comprised of 8 proteins covalently 

coupled to a colored dye, including one orange and one green 

band, allowing easy visualization during electrophoresis with 

two main reference points. By covalently coupling the dye to the 

protein, the electrophoretic behavior of proteins is influenced. 

Due to this behavior change of pre-stained proteins, Protea’s 

Protein Molecular Weight Marker (MM-101-1) should be used 

when specific molecular weight determination is desired. 

FEATURES: 

• Reproducible markers 

• Wide MW range from 7 to 175 kDa 

• Real-time visualization of separation during electrophoresis 


• SDS-PAGE separation of protein samples 

• 1D and 2D gel electrophoresis 

• Western blotting 

SDS-PAGE separation of Protea’s TriColor Pre-stained Protein 

Molecular Weight Marker. 


TriColor Pre-stained Protein Molecular Weight Markers 

MW Range 7-175 kDa, 1 Vial 


MM-110-1 1 vial $126 

Protein Molecular Weight Markers 

Our Protein Molecular Weight Markers are designed for use in 

all standard acrylamide separations of protein samples. Protea’s 

protein marker offers a wide molecular weight range (6.5 - 200 

kDa) with excellent run-to-run reproducibility. Each vial contains 

enough Protein Molecular Weight Marker for 30 gel lanes when 

using Coomassie blue stain and 60 gel lanes when using silver 

stain. We recommend the use of our ProteaGel acrylamide 

solutions with our Protein Molecular Weight Markers for your 

protein separation needs. 

FEATURES: 

• Broad MW range: 6.5 - 200 kDa 

• Lyophilized for long shelf-life 


• SDS-PAGE separation of protein samples 

• 1D and 2D gel electrophoresis 


Protein Molecular Weight Markers 


MM-101-1 1 vial $58 

MW 

200 

116 

97 

66 

55 

45 

36 

29 

24 

20 

14 

6.5 

Protein 

Myosin 

Albumin, bovine serum 

Glutamic dehydrogenase 

Ovalbumin, chicken egg 

Glyceraldehyde- 

3-phosphate 

dehydrogenase 

Carbonic anhydrase 

Trypsinogen 

Soybean trypsin inhibitor 

β-galactosidase 

Phosphorylase-b 

α-lactalbumin 

Aprotinin 

SDS-PAGE separation of Protea’s Protein Molecular Weight 

Markers. Molecular weight is in kilodaltons (kDa). 


iPAGE 300V Power Supply 

The iPAGE 300V Power Supply can run up to 4 gel boxes at a 

time with electrophoresis voltages of 0 to 300 V (0 to 500 mA 

current and 90 watt total power output rating). This versatile 

power supply can be operated in either constant voltage or 

constant current modes, and can be used for protein, DNA, and 

RNA electrophoresis experiments. The digital LCD display clearly 

shows the applied voltage and can be toggled to display current. 

The iPAGE 300V Power Supply also features a timer function 

that can be used to prevent running samples off of the bottom of 

the gel. At the end of the programmed run time, the power supply 

shuts off to protect sample integrity. The iPAGE 300V Power 

Supplies are compact in design with a small footprint and are 

stackable to save space. 

FEATURES: 

• Versatile power supply available with timer function for 

running up to four protein, DNA, or RNA gels 


iPAGE 300V Power Supply 


PG-510 ea. $625 


13 • 877.776.8321

Notes: 


Notes: 


15 • 877.776.8321

© 2011 Protea Biosciences, Inc. • 877.776.8321 • www.proteabio.com

Gel Protein Separation & Recovery - Protea Biosciences

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