Biochemistry NPD Review - Protea Biosciences

MATERIAL SAFETY DATA SHEET
Section 1.
Product and Company Information
Product Name:
Silver Stain Kit (Enhancer)
Catalog Numbers: SS-100-kit / SS-101-kit
UN Number: 2209
Harmonized Code: 2912110000
Revision Date: 08/14/2009
Company:
Protea Biosciences, Inc.
955 Hartman Run Road
Morgantown, WV 26507 USA
P: 304.292.2226 ● F: 304.292.7101
www.proteabio.com
Section 2.
Composition / Information on Components
Chemical Characterization
Description: Mixture of the substances below with non-hazardous additions.
Listing of dangerous and non-hazardous components:
CAS # Chemical Name EC # Percent
30425-89-4 Formaldehyde 200-001-8 30-40%
Section 3.
Hazards Identification
EMERGENCY OVERVIEW
Appearance: colorless liquid
Caution! May cause eye, skin, and respiratory tract burns.
Ingestion: Harmful by ingestion
Inhalation: Harmful by inhalation
Chronic: Danger of serious damage to health by prolonged exposure through inhalation, skin contact, and ingestion.
Information pertaining to particular dangers for man and environment: not applicable
Classification system
The classification was made according to the latest editions of the EU-lists.
NFPA ratings (scale 0 to 4)
Health = 3
Fire = 0
Reactivity = 0
Section 4.
First Aid Measures
General Information: No special measures required.
Eyes: Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get
medical aid.
Skin: Get medical aid if irritation develops or persists. Wash clothing before reuse. Flush skin with plenty of soap and
water.
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Treat symptomatically and supportively.
Get medical aid immediately.
Inhalation: Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.
Notes to Physician: Treat symptomatically and supportively.

Section 5.
Fire Fighting Measures
General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH
(approved or equivalent), and full protective gear. Dusts at sufficient concentrations can form explosive mixtures with
air. Combustion generates toxic fumes.
Extinguishing Media: Use water spray, dry chemical, carbon dioxide, or chemical foam.
Flash Point: Not available.
Section 6.
Accidental Release Measures
General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: A vacuum or sweep up material and place into a suitable disposal container. Clean up spills
immediately, observing precautions in the Protective Equipment section. Do not allow to enter sewers, surface, or
ground water. Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Section 7.
Handling and Storage
Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Ground and
bond containers when transferring material. Avoid contact with eyes, skin, and clothing. Empty containers retain
product residue, (liquid and/or vapor), and can be dangerous. Keep container tightly closed. Do not ingest or
inhale. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or
open flames. Use only with adequate ventilation. Keep away from heat, sparks and flame. Avoid use in confined
spaces. Avoid breathing vapor or mist.
Storage: Keep away from heat, sparks, and flame. Keep away from sources of ignition. Store reagent at room
temperature away from incompatible substances. Keep containers tightly closed.
Section 8.
Exposure Control / Personal Protection
Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a
safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits:
Formaldehyde, 50-00-0
PEL
0.75 ppm TWA
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face
protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use
a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or
other symptoms are experienced.
Section 9.
Physical and Chemical Properties
Physical State: liquid
Appearance: colorless
Odor: odorless
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate: Not available.
Boiling Point: Not available.
Melting Point: Not available.
Decomposition Temperature: Not available.
Solubility: Fully miscible in water.
Specific Gravity/Density: Not available.
Molecular Formula: Not available
Molecular Weight: Not available

Section 10.
Stability and Reactivity
Chemical Stability: Stable under normal temperatures and pressures.
Thermal decomposition / conditions to be avoided: Incompatible materials, dust generation, excess heat.
Dangerous reactions: No dangerous reactions known.
Hazardous Decomposition Products: Will not occur.
Section 11.
Toxicological Information
Silver Stain Enhancer
RTECS#:
CAS# 50-00-0
LD50/LC50:
LD50 Oral 100 mg/kg Rat
LD50 Dermal 270 mg/kg Rabbit
Carcinogenicity:
CAS# 50-00-0: Suspected Carcinogen
Epidemiology: No data available.
Teratogenicity: No data available.
Reproductive Effects: No data available.
Mutagenicity: No data available.
Neurotoxicity: No data available.
Section 12.
Ecological Information
Ecotoxicity: No data available. No information available.
Environmental: Water hazard class 1: slightly hazardous for water. Do not allow undiluted product or large
quantities of it to reach ground water, water course, or sewage system.
Physical: No information available.
Section 13.
Disposal Considerations
Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US
EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators
must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.
Section 14.
Transport Information
DOT Regulations:
Hazard Class: 8 (Corrosive)
Land transport ADR/RID (cross border):
ADR/RID Class: -
Maritime transport IMDG:
IMDG Class: 8
Marine Pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: 8
Transport / Additional information: Not dangerous according to the above specifications.

Section 15.
Regulatory Information
US Federal
TSCA
CAS: 50-00-0 is listed on the TSCA Inventory
SARA Reportable Quantities (RQ)
The components are on this list
CERCLA/SARA Section 313
The components are on this list
OSHA – Highly Hazardous
None of the components are on this list
US State
State Right to Know
CAS# 50-00-0 is present on state lists from CA, PA, MN, MA, FL, or NJ.
European/International Regulations
Canada Ingredient Disclosure List
CAS# 50-00-0 is listed on Canada’s Ingredient Disclosure List.
Section 16.
Other Information
MSDS Creation Date: 08/14/2009
Revision #1 Date: 08/14/2009
The information above is believed to be accurate and represents the best information currently available to us.
However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such
information, and we assume no liability resulting from its use. Users should make their own investigations to
determine the suitability of the information for their particular purposes. In no event shall Protea Biosciences be liable
for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental,
consequential or exemplary damages, howsoever arising, even if Protea Biosciences has been advised of the
possibility of such damages.