Activity report from Indonesia - APRSAF
Activity report from Indonesia - APRSAF
Activity report from Indonesia - APRSAF
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Sample isolation and RNA extraction<br />
Banana fingers were collected and cut into small cubes.<br />
Immediately after cut, cubes were frozen in liquid nitrogen<br />
and placed in -80 o C freezer. Samples were then proceed for<br />
RNA isolation until step 8 (LiCl incubation at 4 o C) and<br />
transported to Indonesa.<br />
The samples were then proceed immediately after arrive in<br />
<strong>Indonesia</strong> (stored for about 7 hr in 4 o C after arrive, 0.30 am-<br />
7.30 am). The RNA extraction was finished on 24 Jan 08.<br />
The quality of RNA was analyzed using spectrophotometer<br />
and agarose gel. However, the ratios of A260/280 were around<br />
1.0. In addition, electrophoresis result showed that RNA <strong>from</strong><br />
sample was degraded. Next time, RNA isolation should be<br />
finished on site to minimize RNA degradation
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