The Importance of DNA Isolation
The Importance of DNA Isolation
The Importance of DNA Isolation
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Spectrophotometry<br />
• Concentration = OD 260 * 50 µg/ml<br />
(ds<strong>DNA</strong>) * dilution factor<br />
• Purity<br />
– Measure <strong>of</strong> proteins<br />
OD 260 :OD 280 = 1.8<br />
– Measure <strong>of</strong> phenolics/chaotropic salts<br />
OD 260 :OD 230 > 1.5<br />
– Measure <strong>of</strong> particulates<br />
OD 330<br />
• Simple & non-destructive,<br />
• Narrow range 5 µg/ml to 90 µg/ml,<br />
easy to over estimate due to<br />
contaminates (RNA, ss<strong>DNA</strong>,<br />
nucleotides, phenols, proteins)
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