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The Importance of DNA Isolation

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Dissolving Membranes<br />

• Lysis by way <strong>of</strong>:<br />

– Detergents<br />

• SDS<br />

• CTAB<br />

– Chaotropic Salts<br />

– Alkaline Lysis<br />

– Other denaturing reagents<br />

Results in a “soup” <strong>of</strong> cellular debris<br />

and the content <strong>of</strong> the cytoplasm.

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