The Importance of DNA Isolation
The Importance of DNA Isolation
The Importance of DNA Isolation
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Fluorometry<br />
• Detection <strong>of</strong> enhanced<br />
fluorescence upon dye binding<br />
ds<strong>DNA</strong><br />
• Hoechst 33258<br />
– Quantitate to 10ng/ml<br />
• PicoGreen<br />
– Quantitate to 25pg/ml, and less<br />
sensitive to the presence <strong>of</strong><br />
contaminants (RNA, proteins,<br />
detergents)
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