The Importance of DNA Isolation

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Input raw fluorescence

values for standards.

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Fluorometry • Detection of enhanced fluorescence upon dye binding dsDNA • Hoechst 33258 – Quantitate to 10ng/ml • PicoGreen – Quantitate to 25pg/ml, and less sensitive to the presence of contaminants (RNA, proteins, detergents)

Input raw fluorescence values for standards.

Page 1 and 2: From the Seed Sample to DNA II: DNA
Page 3 and 4: Also, PCR efficiency is affected by
Page 5 and 6: Isolating DNA… Grinding: mechanic
Page 7 and 8: Grinding • Effects of particle si
Page 9 and 10: Pulling Out… Pulling In… Separa
Page 11 and 12: • Silica Column Columns - DNA bin
Page 13 and 14: Cleaning and Elution • Alcohol wa
Page 15: Spectrophotometry • Concentration
Page 19 and 20: Input raw fluorescence values for u
Page 21 and 22: Volume Initial, Concentration Final

The Importance of DNA Isolation

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