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Germination of wheat grains from uneven microwave heating in an ...

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Fig. 1. Experimental setup. 1 - gra<strong>in</strong> <strong>in</strong>let, 2 - control<br />

p<strong>an</strong>el, 3 - <strong>microwave</strong> chamber, 4 - conveyor,<br />

5 - thermal camera, 6 - data acquisition system<br />

It is difficult to determ<strong>in</strong>e the suitability <strong>of</strong> a <strong>microwave</strong><br />

system for a particular application <strong>in</strong> the gra<strong>in</strong> <strong>in</strong>dustry due to<br />

other quality deteriorations. Non-uniformity <strong>of</strong> <strong>heat<strong>in</strong>g</strong> dur<strong>in</strong>g<br />

<strong>microwave</strong> treatment produces hot spots (localized elevated<br />

temperature), <strong>an</strong>d this may be one <strong>of</strong> the import<strong>an</strong>t factors for<br />

the quality deterioration <strong>of</strong> <strong>gra<strong>in</strong>s</strong>. The non-uniformity (∆T, the<br />

difference between maximum <strong>an</strong>d m<strong>in</strong>imum temperatures) <strong>of</strong><br />

surface temperatures <strong>of</strong> <strong>wheat</strong> (50 g samples) after <strong>microwave</strong><br />

treatment was found to be <strong>in</strong> the r<strong>an</strong>ge <strong>of</strong> 62.9 to 69.5 o C after<br />

expos<strong>in</strong>g to 500 W for 56 s (M<strong>an</strong>ickavasag<strong>an</strong> et al. 2006). Study<br />

on quality <strong>of</strong> the gra<strong>in</strong> samples <strong>in</strong> the hot-spot <strong>an</strong>d the normal<br />

<strong>heat<strong>in</strong>g</strong> zones after <strong>microwave</strong> treatment would help to<br />

underst<strong>an</strong>d the thermal degradation <strong>of</strong> bulk gra<strong>in</strong> dur<strong>in</strong>g<br />

<strong>microwave</strong> treatment. The objective <strong>of</strong> this research was to<br />

determ<strong>in</strong>e the germ<strong>in</strong>ation percentage for <strong>wheat</strong> samples<br />

collected <strong>from</strong> the hot-spot <strong>an</strong>d normal <strong>heat<strong>in</strong>g</strong> zones <strong>of</strong> bulk<br />

gra<strong>in</strong> after <strong>microwave</strong> treatment.<br />

MATERIALS <strong>an</strong>d METHODS<br />

Microwave treatment<br />

A laboratory scale, cont<strong>in</strong>uous type, <strong>in</strong>dustrial <strong>microwave</strong> dryer<br />

(2450 MHz) operated at 230 VAC, 60 Hz, 23 A (Model No:<br />

P24YKA03, Industrial Microwave Systems, Morrisville, NC)<br />

was used <strong>in</strong> this study. The <strong>microwave</strong> dryer consisted <strong>of</strong> a<br />

conveyer-belt assembly, <strong>microwave</strong> applicator, f<strong>an</strong>, <strong>an</strong>d a<br />

control p<strong>an</strong>el (Fig. 1). The speed <strong>of</strong> the conveyor <strong>an</strong>d the power<br />

output <strong>of</strong> the <strong>microwave</strong> generator could be adjusted to the<br />

desired level. The f<strong>an</strong> was on at all times dur<strong>in</strong>g the experiments<br />

<strong>an</strong>d the air <strong>in</strong>let temperature was set at 30 o C.<br />

C<strong>an</strong>adi<strong>an</strong> hard red spr<strong>in</strong>g <strong>wheat</strong> (obta<strong>in</strong>ed <strong>from</strong> the Cereal<br />

Research Centre, Agriculture <strong>an</strong>d Agri-Food C<strong>an</strong>ada, W<strong>in</strong>nipeg,<br />

M<strong>an</strong>itoba) was conditioned to four different moisture levels (12,<br />

15, 18, <strong>an</strong>d 21% wet basis) <strong>an</strong>d used <strong>in</strong> this study. In each<br />

experiment, a 50 g sample was spread on the conveyor <strong>an</strong>d the<br />

top surface was made flat. Then the gra<strong>in</strong> was allowed to enter<br />

the chamber where it was subjected to <strong>microwave</strong> treatment.<br />

The approximate volume <strong>of</strong> the gra<strong>in</strong> sample on the conveyor<br />

Fig. 2. Typical thermogram <strong>of</strong> <strong>wheat</strong> sample show<strong>in</strong>g hot<br />

spot after <strong>microwave</strong> treatment.<br />

dur<strong>in</strong>g treatment was 300×30×10 mm (length <strong>of</strong> the belt × width<br />

<strong>of</strong> the belt × depth <strong>of</strong> gra<strong>in</strong> on the belt). Two <strong>microwave</strong><br />

exposure times were achieved by ch<strong>an</strong>g<strong>in</strong>g the speed <strong>of</strong> the<br />

conveyor. Microwave treatment was given at five power levels<br />

(100, 200, 300, 400, <strong>an</strong>d 500 W) <strong>an</strong>d two exposure times (28<br />

<strong>an</strong>d 56 s). A thermal camera (Model: ThermaCAM TM SC500,<br />

spectral r<strong>an</strong>ge: 7.5 to 13.0 µm, FLIR Systems, Burl<strong>in</strong>gton, ON)<br />

was set to view the gra<strong>in</strong> sample on the conveyor as soon as it<br />

came out <strong>from</strong> the <strong>microwave</strong> chamber after treatment <strong>in</strong> order<br />

to sample <strong>from</strong> the hot-spot <strong>an</strong>d normal <strong>heat<strong>in</strong>g</strong> zones. Samples<br />

were collected us<strong>in</strong>g a spoon while view<strong>in</strong>g the live thermal<br />

images on the monitor <strong>of</strong> the data acquisition computer <strong>an</strong>d the<br />

gra<strong>in</strong> samples were stored <strong>in</strong> polyethylene bags separately for<br />

further <strong>an</strong>alysis. The f<strong>in</strong>al moisture content after <strong>microwave</strong><br />

treatment was measured for the <strong>wheat</strong> samples (bulk).<br />

Thermal imag<strong>in</strong>g<br />

In thermal imag<strong>in</strong>g, radiation pattern <strong>of</strong> <strong>an</strong> object (temperature)<br />

is converted <strong>in</strong>to a visible image. The color <strong>of</strong> each pixel <strong>in</strong> a<br />

thermal image represents a temperature value which is given on<br />

the temperature scale (right side <strong>of</strong> Fig. 2). Generally, <strong>in</strong> a<br />

thermal image, bright <strong>an</strong>d dark colors represent high <strong>an</strong>d low<br />

temperatures, respectively. Because <strong>of</strong> non-uniform <strong>heat<strong>in</strong>g</strong>, hot<br />

spots were observed (as patches) at one or two locations on the<br />

surface <strong>of</strong> <strong>wheat</strong> samples after <strong>microwave</strong> treatment. Samples<br />

were collected <strong>from</strong> the hot spot <strong>an</strong>d the rema<strong>in</strong><strong>in</strong>g region, <strong>an</strong>d<br />

subjected to a germ<strong>in</strong>ation test.<br />

<strong>Germ<strong>in</strong>ation</strong> test<br />

The germ<strong>in</strong>ation test was conducted for the samples collected<br />

<strong>from</strong> the hot-spot <strong>an</strong>d normal <strong>heat<strong>in</strong>g</strong> zones. It was not possible<br />

to conduct bak<strong>in</strong>g quality tests s<strong>in</strong>ce the qu<strong>an</strong>tity <strong>of</strong> sample<br />

collected <strong>from</strong> the hot spot was small (about 10 to 15 g <strong>from</strong><br />

50 g <strong>of</strong> the <strong>microwave</strong>-treated sample). However, to detect<br />

quality degradation <strong>of</strong> gra<strong>in</strong> due to high temperature, a<br />

germ<strong>in</strong>ation test c<strong>an</strong> be used because it is a sensitive, simple,<br />

<strong>an</strong>d reproducible test <strong>an</strong>d the results are reasonably correlated<br />

with bak<strong>in</strong>g tests (Ghaly <strong>an</strong>d Taylor 1982).<br />

Wheat kernels (25 seeds) were placed on Whatm<strong>an</strong> no. 3<br />

filter paper <strong>in</strong> a 90-mm diameter Petri-dish saturated with<br />

5.5 mL <strong>of</strong> distilled water. The Petri-dishes were covered with a<br />

polyethylene bag <strong>an</strong>d kept at 25 o C for 7 d. The germ<strong>in</strong>ated seeds<br />

were counted on the seventh day <strong>an</strong>d germ<strong>in</strong>ation percentage<br />

3.24<br />

LE GÉNIE DES BIOSYSTÈMES AU CANADA MANICKAVASAGAN, JAYAS <strong>an</strong>d WHITE

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