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88 ABSTRACT #P-J5 BARRIERS TO COMPLIANCE WITH COLPOSCOPY/FOLLOW-UP IN A POPULATION OF PATIENTS WITH PAP SMEAR ABNORMALITIES: A SURVEY APPROACH. Andrea N. Simpson [M](1), Catriona J. Buick(2), K. Joan Murphy(2) (1)University of Toronto, (2)Department of Gynaecologic Oncology, Princess Margaret Hospital, University of Toronto. Objective: To determine the underlying health beliefs and demographic, lifestyle, psychological, and social factors that predict patterns of compliance or non-compliance to follow-up among women requiring care for Pap smear abnormalities. Methods: A comparative survey design method will be used to examine thirty women who have been referred to Princess Margaret Hospital/University Health Network (UHN) in Toronto, Ontario, for an abnormal Pap smear. On completion of consent and the survey, women who have missed one or more scheduled appointments will be assigned to the study group. Women who have complied with appointments will be assigned to the contrast group. The survey has been adapted from a previously validated survey, the Mammography Screening Beliefs Questionnaire, and is based on the Health Belief Model. The survey will also encompass demographic information, a brief health history, and an open-ended qualitative portion where participants may address their specific concerns. The study was approved by the UHN Research Ethics Board in November 2008. Results: Data will be analyzed for common themes and for differences between the group of women who attended their scheduled appointment(s) and those who did not. Conclusions: To our knowledge, barriers to compliance with follow-up for abnormal Pap smears have never been studied in a diverse population of women in a large metropolitan area, specifically the Greater Toronto Area. The information collected in this study is valuable in future program planning to manage the needs of the target population(s) and potentially improve adherence to, and thus efficacy of, recommended screening algorithm.
89 ABSTRACT #P-K1 DIFFERENTIATION OF HEPATOCYTES FROM HUMAN FIRST TRIMESTER UMBILICAL CORD STEM CELLS Junhai Zhao [PD], Rong Xiao, Shangmian Yie and Clifford L. Librach Department of Obstetrics and Gynaecology, Sunnybrook Health Sciences Centre, Women’s College Hospital, University of Toronto. Objective: We have previously isolated and expanded stem cells from the first trimester human umbilical cord (UCSC) and have shown that these cells have embryonic stem cell–like characteristics. We have demonstrated that these UCSC’s have the ability to form embryoid bodies (EBs) in vitro, and have the capacity to differentiate into a wide variety of cell types which demonstrate characteristics of all three embryonic germ layers (such as neuroectoderm, cardiomyocyte, osteogenic, adipogenic mesoderm and pancreatic endoderm). We report here a robust and efficient process for the production of hepatocyte-like cells from first trimester UCSC’s in vitro. Methods: After formed EBs in suspension culture conditions, UCSC’s were transferred into an endoderm culture medium. Five days later, definitive endoderm cells (DE) were then induced to differentiate using factors involved in early hepatic development, on a Collagen I matrix in serumfree media. Morphologic changes were examined at each step. Expression of hepatic markers was analyzed using RT-PCR and Immunocytochemistry (ICC). Results: During culture of UCSC’s in hepatocyte differentiation conditions, the cultures remained mostly homogeneous aside from some cells appearing to be smaller and closer together. As the cells matured, on day 20 th , they developed cytoplasmic vacuoles resembling confluent cultures of polygonal vacuolated primary human hepatocytes. The expression profile of hepatic lineage genes through the time course of differentiation was examined. Alpha Fetoprotein (AFP) and Albumin (ALB) were detectable at day 11 and beyond. Expression of the hepatic maturation and transcription genes, Cyp3a4 and Cyp7a1, was found on day 20 of culture. Conclusions: We have demonstrated that first trimester UCSC’s cells represent an embryonic-like stem cell population with the capacity to differentiate into cells that demonstrate key 1)morphologic changes, 2)gene expression and 3)protein expression found in mature hepatocytes.
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Department of Obstetrics and Gynaec
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2 PROGRAMME-AT-A-GLANCE (A.M.) 7:30
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5 PROGRAMME 26TH ANNUAL RESEARCH DA
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7 P-A4 PHENOTYPE OF EARLY ONSET INT
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9 P-C2 ESTROGEN EXTRACTION FROM MIC
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11 P-E4 LAPAROSCOPIC PERITONEAL ENT
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13 12:05 - 1:05 p.m. LUNCH [Dining
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15 P-G2 P16 ONCOPROTEIN AS A TRIAGE
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17 GROUP I: Chairs/Judges: Drs. Jon
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19 GROUP K: Chairs/Judges: Drs. Gra
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21 4:15 - 5:15 p.m. Henderson Lectu
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23 AWARDS The Papsin Award The Dr.
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26 ABSTRACT #O1* CO-ENZYME Q10 SUPP
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28 ABSTRACT #O3* VEPH1 IS A NOVEL R
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30 ABSTRACT #O5* TISSUE SPECIFIC TR
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32 ABSTRACT #O7 JOB SATISFACTION AM
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34 ABSTRACT #O9 ABDOMINAL VISCERAL
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36 ABSTRACT #O11 REGULATORY EFFECTS
Page 40 and 41: 38 ABSTRACT #O13 THE EFFECT OF CHRO
Page 42 and 43: POSTER ABSTRACTS 40
Page 44 and 45: 42 ABSTRACT #P-A2 INCREASED MATERNA
Page 46 and 47: 44 ABSTRACT #P-A4 PHENOTYPE OF EARL
Page 48 and 49: 46 ABSTRACT #P-B1* ANGIOGENIC CD56
Page 50 and 51: 48 ABSTRACT #P-B3 UNDERSTANDING THE
Page 52 and 53: 50 ABSTRACT #P-B5 LOW VITAMIN D LEV
Page 54 and 55: 52 ABSTRACT #P-C2* ESTROGEN EXTRACT
Page 56 and 57: 54 ABSTRACT #P-C4 PRETERM PERMATURE
Page 58 and 59: 56 ABSTRACT #P-D2 CRYOPRESERVATION
Page 60 and 61: 58 ABSTRACT #P-D4 DIFFERENTIAL EXPR
Page 62 and 63: 60 ABSTRACT #P-E1 THE ROLE OF ANDRO
Page 64 and 65: 62 ABSTRACT #P-E3 CELL CYCLE INHIBI
Page 66 and 67: 64 ABSTRACT #P-F1 DEXAMETHASONE STI
Page 68 and 69: 66 ABSTRACT #P-F3 EDUCATING SURGICA
Page 70 and 71: 68 ABSTRACT #P-F5 OUTCOMES IN ROBOT
Page 72 and 73: 70 ABSTRACT #P-G2 P16 ONCOPROTEIN A
Page 74 and 75: 72 ABSTRACT #P-G4 ALTERED ADRENOCOR
Page 76 and 77: 74 ABSTRACT #P-H1 RETINAL HEMORRHAG
Page 78 and 79: 76 ABSTRACT #P-H3 A ROLE FOR MCL-1
Page 80 and 81: 78 ABSTRACT #P-H5 A NOVEL ROLE FOR
Page 82 and 83: 80 ABSTRACT #P-I2 THE TIMING OF DEL
Page 84 and 85: 82 ABSTRACT #P-I4 OMEGA-3 FATTY ACI
Page 86 and 87: 84 ABSTRACT #P-J1* WHAT IS KEEPING
Page 88 and 89: 86 ABSTRACT #P-J3 HUMAN EMBRYONIC F
Page 92 and 93: 90 ABSTRACT #P-K2 THE “DUC” TRI
Page 94 and 95: 92 ABSTRACT #P-K4 IS THERE A ROLE F
Page 96 and 97: 94 ABSTRACT #P-L1 EXPRESSION AND RE
Page 98 and 99: 96 ABSTRACT #P-L3 IS ZONA PELLUCIDA
Page 100 and 101: 98 ABSTRACT #P-L5 SPECTRUM OF VASCU
Page 102 and 103: 100 Presenters by Last Name cont’
Page 104 and 105: 102 Presenters by Abstract # and Se
Page 106 and 107: 104 Presenters by Abstract # and Se
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