Vol 31, Part I - forums.sou.edu • Index page - Southern Oregon ...

ABSTRACTS – Symposia
osteoblasts are affected by changes in inflammtory signaling
and the extracellular matrix and cause changes in turn. This
data may provide insight into processes with relevance to
diverse issues in human health, from cancer to space travel.
18 Going Green in Space KEITH LAMPI (Hydration Technology
Innovations, LLC, Vice President of Operations, 2484
Ferry St. SW, Albany, OR 97322; klampi@htiwater.com).
Hydration Technology Innovations has worked with NASA
for 15 years providing materials and research for life support
systems focusing on water reuse. The brief talk will relay these
findings and discuss technology transfer for both industrial and
humanitarian opportunities and conclude with the results of
testing of an emergency hydration pouch by Commander Chris
Ferguson on the Space Shuttle Atlantis (STS 135).
19 Calcium Flux During Cell-Cell Communication – BSU
Microgravity University 2012, REILLY CLARK, Lindsey
CATLIN*, Landon Nye, Kellen MATHER,
TRAVIS Baker, DAVID CONNOLLY, MATTHEW
Dolan, Jason Archer, EUGENE CASTRO,
Audra Phelps, Nic baughman, Daniel Lambert,
Marie Tharp, Joshua Anghel, Benjamin
DAVIS, ROBERT HAY, ALARK JOSHI, Sarah
haight, elisa Barney smith, Julia Oxford,
and BARBARA MORGAN (Department of Biological
Sciences, Department of Electrical and Computer Engineering,
Department of Mathematics, Department of Mechanical
and Biomedical Engineering, Department of Business
Management, Department of Computer Science, Division
of Research, Boise State University, Boise, ID 83725;
reillyclark@u.boisestate.edu)
Osteocytes integrate mechanical information into chemical
signals relayed to osteoclast and osteoblast cell populations.
In effect, these signals orchestrate bone resorption and
formation by the osteoclasts and osteoblasts, respectively.
While these activities are essential for the maintenance of
healthy bone, imbalances in these processes by exposure to
extreme environments, such as microgravity, are hypothesized
to lead to highly detrimental bone loss. Changes in
free calcium concentration, known as calcium flux, is an
intermediate step in the chemical signaling processes of the
osteoctyes. To determine how environments of continually
alternating forces affect the bones of the human body, it is
important to study how those environments affect calcium
flux. To this end, this experiment examined how osteocyte
and osteoblast mono- and co-cultures respond to the periods
of micro-and hyper-gravity experienced onboard NASA’s
Weightless Wonder. Calcium flux in the three cell cultures
was fluorescently monitored through the use of a lens and
imaging-based system. The cellular calcium concentrations
increased during periods of hyper-gravity and decreased during
periods of microgravity.
20 Device Design and Development for Imaging Cellular
Behavior – BSU Microgravity University 2012, DAVID
CONNOLLY, MATTHEW Dolan*, Jason Archer,
EUGENE CASTRO, REILLY CLARK, Kellen
MATHER, Lindsey CATLIN, Landon Nye, Travis
Baker, Audra Phelps, Nic Baughman,
Daniel LAMBERT, Marie Tharp, Joshua Anghel,
Benjamin DAVIS, Alark Joshi, ROBERT
Hay, Sarah haight, elisa Barney smith,
BARBARA MORGAN, and Julia Oxford (Department
of Electrical and Computer Engineering, Department
of Mechanical and Biomedical Engineering, Department of
Computer Science, Department of Business Management,
Department of Mathematics, Department of Biological Sciences,
Division of Research, Boise State University, Boise,
ID 83725; davidconnolly@u.boisestate.edu).
This year’s Boise State Microgravity University team is
building a system which excites osteocyte mono-cultures and
osteocyte-osteoblast co-cultures at 350nm and then images
the Indo-1 dye at 485nm and 405nm based on whether
the dye is unbound or bound to calcium, respectively. In
order to image these wavelengths, the system built needed
enough resolution to image 96 5mm wells in a 3x5 inch 96
well microtiter plate. Another complexity of the system is
that it needed to image these wells during a 30s period of
micro or hyper gravity, requiring the exposure time and time
between captures to be small. The imager itself needed to be
sensitive enough that it can capture the wavelengths without
introducing unnecessary noise even though the signal does
not have significant luminosity. Two mono-chrome imagers
using C-mount band-pass filters were used in order to isolate
the 405nm and 485nm emissions simultaneously. Capturing
images was done using Aptina proprietary software and C
programming language utilizing multi-threading to capture
images from both cameras simultaneously. After capturing
the RAW images, the edges of each cell were detected, and
the contents were analyzed to determine how much luminosity
from each well was caused by unbound or bound dye,
allowing a quantification of free calcium.
Forensic Psychology in Evaluating
a Lone Wolf Terrorist: An Analysis of
the Norway Killer
Monday, 8:40 a.m. in PAYETTE RIVER
21 Overview and Questions for Forensic Psychology in Homegrown
Lone Wolf Terrorism Cases, RONN JOHNSON (Clinical
Mental Health Program, University of San Diego, 5998
Alcalá Park, San Diego, CA, 92110; ronnjohn@sandiego.edu).
Police and public safety personnel often make decisions
designed to protect citizens. These decisions are frequently
made with a degree of uncertainty when it comes to acts of
terror. Nonetheless, clinical mental health professionals are
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