Regulation of Enzyme Activity - mdk12
Regulation of Enzyme Activity - mdk12
Regulation of Enzyme Activity - mdk12
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“<strong>Enzyme</strong> Action: Testing Catalase <strong>Activity</strong>”<br />
Rationale:,<br />
• To explore the effect <strong>of</strong> changes in enzyme concentration, temperature, and<br />
pH on enzyme activity<br />
Time:<br />
• Approximately 45 minutes<br />
Objectives:<br />
• To test the effect <strong>of</strong> the concentration <strong>of</strong> catalase on the enzymatic<br />
breakdown <strong>of</strong> hydrogen peroxide<br />
• To test the effect <strong>of</strong> pH on the enzymatic breakdown <strong>of</strong> hydrogen peroxide<br />
• To test the effect <strong>of</strong> temperature on the enzymatic breakdown <strong>of</strong> hydrogen<br />
peroxide<br />
Materials:<br />
• CBL/TI system<br />
• Vernier Biology Gas Pressure Sensor<br />
• temperature probe or thermometer<br />
• hydrogen peroxide<br />
• fresh calf’s liver<br />
• water bath<br />
• pH buffers (4, 7, and 10)<br />
• see lab for additional materials<br />
Overview:<br />
• Student groups will measure the effect <strong>of</strong> enzyme concentration, pH, or<br />
temperature on the breakdown <strong>of</strong> hydrogen peroxide by catalase.<br />
• Data will be shared among groups.<br />
• Students will compare experimental results to conditions normally found in the<br />
human body.<br />
Procedure:<br />
See Holman, S., Masterman, D. Biology with CBL. “<strong>Enzyme</strong> Action: Testing<br />
Catalase <strong>Activity</strong>.” Vernier S<strong>of</strong>tware, 1997. 6-1 through 6-6.<br />
http://www.vernierkorea.com/exper/sample/bio/101.pdf<br />
V. Evaluation<br />
22
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