A Guide to HPLC & LC-MS Buffer Selection - by John W ... - Hplc.eu
A Guide to HPLC & LC-MS Buffer Selection - by John W ... - Hplc.eu
A Guide to HPLC & LC-MS Buffer Selection - by John W ... - Hplc.eu
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the gap at the low-pH end. Ammonium bicarbonate (6.6 8 are expected <strong>to</strong><br />
be shortened. Of course columns which use polymeric, rather than silica,<br />
particles are not susceptible <strong>to</strong> silica dissolution, so may be used at high pH, but<br />
such columns are not available in as wide a range of stationary phases as silicabased<br />
columns.<br />
How Much<br />
One function of the buffer is <strong>to</strong> keep the analyte at the desired pH. If you<br />
consider just this aspect of buffering, very little buffer is needed. For analytical<br />
work, many samples are in the µg/mL <strong>to</strong> ng/mL range. So for injections of
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