Asahipak - Hplc.eu

Capture the EssenceHPLC Columns and Instruments 2011 – 2012SHOWA DENKO K.K.

TMWe provide a wide range of products to meet your analytical needs, from pretreatment andseparation columns to HPLC-related instruments.Please visit the Shodex website to see application data and uses.Shodex websitehttp://www.shodex.deThe following names are registered trademarks of SHOWA DENKO K.K.Shodex, Asahipak, CLNpak, SUGAR, ORpak, ODP, BIOACTThe following name is registered trademarks of ERC Inc.DEGASSER[Caution]1. Please read the operating manual included on theproduct carefully before use.2. For improvement purposes, some specifications aresubject to change without notice.3. The figures included in this catalogue are not guaranteedand should be used only as references.4. It is essential to take normal precautions when handlingreagents and other chemical products even if the safetyinformation is not included on the operating manual.5. Products described in this brochure are not intended formedical use or medical applications including medicaldiagnosis.

ContentsColumn selectionSample pretreatment columnsStandard analysis columnsReversed phase,Hydrophilic Interaction andNormal phase ChromatographyChiral SeparationIon Exchange ChromatographyHydrophobic Interaction ChromatographyAffinity ChromatographyLigand Exchange ChromatographyIon Exclusion ChromatographyIon ChromatographySize Exclusion ChromatographyCalibration standards for SECApplication fieldsSemi-micro and micro columnsPreparative columnsInstrumentsInformationIndexTechnical information listTypes of columns, base materials, functional groups and ligandsHPLC Separation ModesColumn selection by sample character and separation modeColumn selection (Application)Columns for GPC Clean-upColumns for Column Switching methodPolymer-based Packed Columns for Reversed Phase ChromatographyPolymer-based Packed Columns for Hydrophilic Interaction Chromatography (HILIC)Silica-based Packed Columns for Reversed Phase, HILIC and Normal Phase ChromatographyColumns for Chiral SeparationColumns for Anion Exchange ChromatographyColumns for Cation Exchange ChromatographyColumn for Hydrophobic Interaction ChromatographyColumns for Affinity ChromatographyColumns for Ligand Exchange ChromatographyColumns for Ion Exclusion ChromatographyColumns for Ion Chromatography (Anion analysis)Columns for Ion Chromatography (Cation analysis)Column Selection for Size Exclusion Chromatography (SEC)Aqueous SEC (GFC) columns : Silica-basedAqueous SEC (GFC) columns : Polymer-basedMultimode columnsAqueous/Organic SEC columnsOrganic SEC (GPC) columns (General analysis) : THF, chloroformOrganic SEC (GPC) columns (General analysis) : DMFSolvent-peak separation columns for Organic SEC (GPC)Organic SEC (GPC) columns : Rapid analysis, High Performance AnalysisOrganic SEC (GPC) columns : Linear calibration typeHigh temperature/Ultra high temperature analysis SEC (GPC) columnsOrganic SEC (GPC) columns : HFIPCalibration Standards for SECPharmaceuticals and Cosmetics analysisFoods analysisBiotechnology analysisEnvironment analysisUSP(United States Pharmacopeia) Column ListSemi-micro and micro columnsPreparative columns (I.D. 10mm~50mm)Custom-made Preparative ColumnsRefractive Index Detector Shodex RI-201, RI-201HRefractive Index Detector Shodex RI-101, 102, 104Electric Conductivity Detector Shodex CD-200Dissolved Gas Removal Devices DEGASSER ERC-3215α, 3415αColumn Trouble ShootingPrecautions for polar polymer analysisApplicability of SEC (GPC) columns to solvent replacementColumn Cleaning ProceduresGeneral precautions for column handlingHPLC System Trouble ShootingIndex by product nameTechnical Notebook, Chromato News233468101320222426262832363840424446485052545456585860636465666869708287909192923143628889939496

Column selection2Types of columns, base materials, functional groups and ligandsTypes of columns, base materials,functional groups and ligandsSeparation Type Products Base MaterialGPC Clean-upColumn SwitchingPretreatmentReversed & HILIC(Polymer-based)Reversed & HILIC(Silica-based)Chiral SeparationIon ExchangeHydrophobicAffinityLigand ExchangeIon ExclusionIon ChromatographyAqueous SEC (GFC)MultimodeAqueous/Organic SECCLNpakCLNpakMSpakMSpakPROTEINRSpakRSpakRSpakRSpakRSpakAsahipakAsahipakAsahipakAsahipakODSpakSilicaSilicapakSilicaSilicaSilicaSilicaSilicaSilicaSilicaORpakORpakORpakORpakORpakIECIECIECPIKESSAsahipakAXpakIECIECPIKESSIECAsahipakCXpakHICAFpakSUGARSUGARSUGARRSpakSUGARUSPpakSUGARRSpakICICWINEICICICOHpakPROTEINAsahipakMSpakAsahipakAsahipakMSpakEVPAEPKGF-4AKW-604SRP18, DSDE, GOLF, CARBODP2 HPET-RP1DM-614NNJJ-50ODP-50, ODP-40C8P-50C4P-50NH2PFC18M, C18PE-4115SIL5C85C45CN5NH5NPE5PYECDA-453 HQCDB-453 HQCDC-453 HQCDBS-453CRX-853QA-825DEAE-825DEAE3NDEAE-2BES-502NWA-624SP-825SP-420NSP-2BCM-825ES-502CP-421SPH-81432 kindsSCSP0810KS-800DC-613SZ5532MN-431SHKC-811NI-424, I-524ASIVH-anionYS-50YK-421Y-521, T-521, R-621SB-800 HQSB400KW-800KW400GS-HQGS-320GSGF-HQGF-310Styrene divinylbenzene copolymerPolyvinyl alcoholHydrophilic copolymers containing N-vinyl acetamidePolyvinyl alcoholSilicaStyrene divinylbenzene copolymerPolymethacrylatePolyhydroxymethacrylatePolyvinyl alcoholPolyhydroxymethacrylatePolyhydroxymethacrylatePolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholSilicaSilicaSilicaSilicaSilicaSilicaSilicaSilicaSilicaSilicaPolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylateSilicaPolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylatePolyvinyl alcoholPolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylatePolyvinyl alcoholStyrene divinylbenzene copolymerPolyhydroxymethacrylatePolyhydroxymethacrylateStyrene divinylbenzene copolymerStyrene divinylbenzene copolymerStyrene divinylbenzene copolymerStyrene divinylbenzene copolymerStyrene divinylbenzene copolymerStyrene divinylbenzene copolymerStyrene divinylbenzene copolymerStyrene divinylbenzene copolymerPolyhydroxymethacrylatePolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholSilicaStyrene divinylbenzene copolymerPolyhydroxymethacrylatePolyhydroxymethacrylateSilicaSilicaPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholFunctional Group andligand————Hydrophilic polymer———Octadecyl—SulfoQuaternary ammoniumOctadecylOctylButylAminoOctadecylOctadecyl——OctylButylCyanopropylAminopropylNitrophenylethylPyrenylethylα-Cyclodextrin derivativeβ-Cyclodextrin derivativeγ-Cyclodextrin derivativeβ-Cyclodextrin derivativeL-amino acid derivativeQuaternary ammoniumDiethylaminoethylDiethylaminoethylDiethylaminoethylDiethylaminoethylDiethylaminoethylSulfopropylSulfopropylSulfopropylCarboxymethylCarboxymethylSulfo(Na + )Phenyl32 kinds of ligandSulfo(Ca 2+ )Sulfo(Pb 2+ )Sulfo(Na + )Sulfo(Na + )Sulfo(Zn 2+ )Sulfo(Ca 2+ )SulfoSulfoQuaternary ammoniumQuaternary ammoniumQuaternary ammoniumCarboxylCarboxylSulfo——Hydrophilic polymerHydrophilic polymer—————Page668881111, 121111121212101010132020202020202020202022222222222424242424242626262626262622, 2832, 3332323333323636383838404040464644444848485050Organic SEC (GPC)GPCKF-800, KF-600,KF-400HQ,K-800, KD-800,HFIP-800, HFIP-600,LF, HT-800, UT-800,AT-806MSStyrene divinylbenzene copolymer—52, 54,56, 58,60

HPLC Separation ModesLiquid chromatography (LC) uses liquid as mobile phase (eluent). It is an analytical method that separates a mixture of compoundsbased on their physical and chemical differences. High-performance liquid chromatography (HPLC) is a method that introducesthe mobile phase under high-pressure conditions that results in rapid and high-performance separations. The various interactionsbetween the analyte, stationary phase (packing material), and mobile phase are the key factors for the separation. A wide varietyof separation mechanism can be obtained by using particular combinations of stationary and mobile phases.Separation modeCharacteristicsColumn selectionReversed PhaseChromatography(RPC)Normal PhaseChromatography(NPC)HydrophilicInteractionChromatography(HILIC)Ion ExchangeChromatography(IEC)HydrophobicInteractionChromatography(HIC)AffinityChromatography(AFC)Ligand ExchangeChromatography(LEX)Ion ExclusionChromatography(IEX)IonChromatography(IC)Size ExclusionChromatography(SEC)Chiral Separation(CS)MultimodeChromatography• Separation is based on the partition equilibrium between stationary phase and mobile phase.• The polarity of the stationary phase is lower than the mobile phase.• Typically used mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents(water or buffer).• Using the lower polarity mobile phase trigers a faster elution.• Separation is based on the partition equilibrium between the stationary phase and the mobile phase.• The polarity of the stationary phase is higher than that of the mobile phase.• Typically used mobile phase contains a mixture of organic solvents with different polarities such as hexane and isopropanol.• Using the higher polarity mobile phase trigers a faster elution.• Separation is based on hydrophilic interaction.• A high polarity stationary phase is used.• Typically the mobile phase contains a mixture of organic solvent such as acetonitrile and aqueous solvents (water or buffer).• Using the higher polarity mobile phase trigers a faster elution.• Applicable for the analysis of high polar substances.• Separation is based on electrostatic interactions between the ion exchanger and ionic solutes.• The mobile phase of choice should have a sufficient buffering capacity at the pH that produces the largest chargedifferences between the analyte of interest.• The elution position is optimized by varying the pH, salt concentration, and/or ionic strength of the mobile phase.• Separation is based on hydrophobic interaction.• Hydrophobic functional group is modified on the stationary phase.• Adsorption of analytes generally occurs at a high salt concentration and they are released by lowering the salt concentration.• Suitable for protein analysis.• Separation is based on adsorption of the analyte to the specific biologically derived ligand pair.• Highly selective.• A buffer solution with the appropriate pH and ionic strength is selected based on the type of ligand, analytes, and their interaction.• Mainly used for the purification and concentration of biological active substances.• Separation is based on differences in analytes’ coordination complex.• Stationary phase modified with metal sulfonate complex ion.• Works in the combination with size exclusion and HILIC modes.• Separation is based on electrostatic interaction (repulsion) between the ion exchanger and ionic solutes.• Dissociated ionic molecules elute faster than non-dissociated forms.• Used mainly for the analysis of inorganic compounds.• Separation is based on electrostatic interaction (bonding) between the ion exchanger and ionic solutes.• Has a relatively small ion exchange capacity.• Electrical conductivity detector can be used by employing a low-salt-concentration of mobile phase.• Used mainly for inorganic compound analysis.• The capillary pores on the surface of high-molecular-base gel works as molecular seve to separate molecules based ontheir sizes.• To separate molecules solely based on their sizes, it requires an analytical condition without any analyte and packinggel interaction.• The bigger the molecule size, the faster the elution sequence.• Suitable for molecular weight determination or molecular distribution of macromolecules and qualification of oligomers.• Separation of optical isomers using chiral selectors.• Highly selective.• Separation is based on the combination of different modes.3HPLC Separation ModesColumn selection by sample character and separation modeSamplesolubilityAqueoussolubleSampleMW≥ 2,000≤ 2,000SeparationmodeSECLEXIEXIECHICRPCAFCSECLEXIEXIECICRPCHILICAFCCSSamplesolubilityOrganicsolubleSampleMW≥ 2,000≤ 2,000SeparationmodeSECSECRPCNPCRPC : Reversed phase chromatographyNPC : Normal phase chromatographyHILIC : Hydrophilic interaction chromatographyIEC : Ion exchange chromatographyHIC : Hydrophobic interaction chromatographyAFC : Affinity chromatographyLEX : Ligand exchange chromatographyIEX : Ion exclusion chromatographyIC : Ion chromatographySEC : Size exclusion chromatographyCS : Chiral separation

Column selectionColumn Selection (Application)Pharmaceuticals, CosmeticsSeparation modeHydrophobic substancesRPCPage10,11,20BiotechnologySeparation mode PageIEC+SEC 48PharmaceuticalsMetabolitesHydrophilic substancesHILICIEC+RPCLEX+SEC13,201232NucleobasesNucleotidesNucleosidesIECRPCAFC241128Substances in bio-fluid(serum·plasma·urine)RPCSEC+RPC1148,50GenomicsOligo nucleic acidsIEC+SECIEC4824LEX+HILIC33RPC114PolyalcoholsLEX+SECSEC3246,50DNARNASECAFC46,5028Column Selection (Application) • Pharmaceuticals, Cosmetics • BiotechnologyMoisturizersEmulsifiersPreservativesOptical activematerialsProtein hydrolysatesMucopolysaccharidesSurfactantsParabenDehydroacetic acidRPCSECRPCSECSEC+RPCSECRPCCSSeparation Mode (p.4 and p.5)RPC : Reversed phase chromatographyNPC : Normal phase chromatographyHILIC : Hydrophilic interaction chromatography114410,11,20465052,5610,11,2022ProteomicsGlycomicsHormoneAmino acidsProteinsPeptidesGlycoproteinsSugar chainsMonosaccharidesSialic acidsUronic acidsAldonic acidsAminesIECIEC+IEX+RPCIEC+SECRPCSECIECRPCHICAFCSECIECRPCHICAFCHILICAFCLEX+SECLEX+HILICHILICIEX+SECRPCIECRPC26,4012481044,48,5024,2610,11262844,48,5024,2610,11262813283233133610,112611IEC : Ion exchange chromatographyHIC : Hydrophobic interaction chromatographyAFC : Affinity chromatographySteroidsHILICSEC1346,50LEX : Ligand exchange chromatographyIEX : Ion exclusion chromatographyIC : Ion chromatographySEC : Size exclusion chromatographyCS : Chiral SeparationLipidPhospholipidsLipoproteinsNPCSECSECAFC2050,52,564628

FoodsNutritionalingredientsFood safetyMonosaccharidesDisaccharidesSugar alcoholsOligosaccharidesPolysaccharidesOrganic acidsWater soluble vitaminsFat-soluble vitaminsFatty acidsNucleic acids (umami)Amino acidsFood additivesPesticidesPretreatment ofresidual pesticidesSeparation mode Page Separation mode PageLEX+SEC 32AnionsIC38LEX+HILICHILICSECLEX+HILICHILICSECIEX+RPCICRPCRPCHILICIEC+RPCRPCNPCSECRPCSECIEC+SECIECIEC+IEX+RPCRPCHILICRPCICIEC+RPCSECGPCclean-up331332,46,48331332,4612,36381110,111312102052,5611,2050,52,5648261210,11131238126Water qualitySoilEnvironmentEnvironmentalhormonesBioethanolsOxyhalidesCyanideCyanogen chlorideCationsSurfactantsPesticidesAnionsHeavy metalsRare earthsHumic substancesOrganic arsenicPesticidesPretreatment ofphthalatesPCBsBenzo [a] pyreneMonosaccharidesOligosaccharidesPolysaccharidesOligosaccharidesAlcoholsSaccharidesOrganic acidsAlcoholsHemicellulosesCellulosesICIEXICSEC+RPCRPCRPCICIEC+RPCICICICSECIEX+RPCRPCICIEC+RPCSECGPCclean-upLEX+SECHILICLEX+SECIEX+RPC+SECSEC3836405020123812384040461212381263213323654,58Column selection Column Seletion (Application) • Foods • Environment • New materials5New materialsSeparation mode PageOrganic solvent soluble50,52,56,58BiodieselsCationsFatty acid glyceridesICSEC4050SyntheticpolymersPolar organic solvent solubleHigh temperature/Ultra-high temperatureSEC46,50,54,5858Fatty acid methyl estersOrganic acidsRPCIC1138Water soluble44,46,48,50RPC10,11,20Additives,Organic solvent soluble50,52,56,58oligomersPolar organic solvent solubleSEC50,54,58Water soluble44,46,48,50

Sample pretreatmentcolumnsColumns for GPC Clean-upFeaturesEV Suitable for fractionation of residual pesticides in foodUsed in the purification process of test solution preparation method in Shoku-An No. 1003001 (October 3rd,2006. Japan) of the Pharmaceutical and Food Safety Bureau, MHLW, Section 2 "Simultaneous GC/MS(LC/MS) Analyses of Agricultural Chemicals in Livestock and Marine Products"PAESuitable for cleaning up water samples such as blood and bottom sedimentHighly effective for fractionation of endocrine disruptors in environmental samples6Columns for GPC Clean-upAppropriate columnsfor GPC clean-upDoes the samplecontain water?NoYesGPC Clean-up for residual pesticides in food, etcEluentEthylacetate/CyclohexaneAcetone/CyclohexaneAcetonitrileAcetoneColumnEV-2000, EV-200EV-2000 ACPAE-2000, PAE-800PAE-2000 AC, PAE-800 ACProduct CodeProduct NameParticle Size(μm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping SolventF6090005CLNpak EV-20016302.0 × 150Ethylacetate/Cyclohexane=3/7F6090001CLNpak EV-2000163020.0 × 300Ethylacetate/Cyclohexane=3/7F6090002CLNpak EV-G16(guard column)20.0 × 100Ethylacetate/Cyclohexane=3/7F6090003CLNpak EV-2000 AC163020.0 × 300Acetone/Cyclohexane=3/7F6090004CLNpak EV-G AC16(guard column)20.0 × 100Acetone/Cyclohexane=3/7Base Material : Styrene divinylbenzene copolymerGPC Clean-up for phthalic acid esters in sediments, biological samples, blood, etcProduct CodeProduct NameParticle Size(μm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping SolventF7600025CLNpak PAE-80054008.0 × 300AcetonitrileF6810022CLNpak PAE-2000540020.0 × 300AcetonitrileF6714007CLNpak PAE-G9(guard column)8.0 × 50AcetonitrileF7600026CLNpak PAE-800 AC54008.0 × 300AcetoneF6810023CLNpak PAE-2000 AC540020.0 × 300AcetoneF6714008CLNpak PAE-G AC9(guard column)8.0 × 50AcetoneBase Material : Polyvinyl alcohol*Contact Shodex or our distributors near you for customized columns.

Preparation of Test Samples Used in Simultaneous GC/MS and LC/MS Analysisof Agricultural Chemicals in Livestock and Marine Products (Outline)Extractionliver, kidneyacetone/n-hexane (1:2)GPCmuscle, fat, fish milk, egg honeyGPCacetonitrilesalting-outGPC Clean-up of Pesticide Residues using EV-2000 ACAcrinathrin (5mg/L)(marker pesticide)Tricyclazole (5mg/L)(marker pesticide)Sample pretreatmentcolumnsPurificationfraction-1[*1] [*1] [*1][*2]fraction-2pesticidefractionpesticidefractionmuscle, fat,fish, milk, eggliver, kidneyfraction-1pesticidefractionfraction-2Acrinathrin (5mg/L)GPC Clean-up of Beef TallowGPC Clean-up of Fatty TunaTricyclazole (5mg/L)test sampleGC/MS, LC/MS*1 purification with Ethylenediamine-N-propylsilyled silica gel mini-column*2 purification with silica gel mini-columnGPC Clean-up of Fatty Tuna and Beef Tallow[Extraction]1) Weigh 20.0g of the sample (5.0g for fat).2) Add 20mL of water and homogenize.3) Add 100mL of acetone/n-hexane (1/2)and homogenize.4) Centrifuge (2,500rpm, 5min.) and collectthe organic layer (1).5) Add 50mL of n-hexane to the residueand homogenize.6) Centrifuge (2,500rpm, 5min.) and collectthe organic layer (2).7) Combine (1) and (2) and add anhydroussodium sulfate for dehydration.8) Remove anhydrous sodium sulfate byfiltering and concentrate the filtrate at40˚C or lower.9) Remove the solvent.10) Weigh the residue (extracted fat).11) Dissolve in acetone/cyclohexane (1/4).Column : Shodex CLNpak EV-G AC + EV-2000 ACEluent : Acetone/Cyclohexane=1/4Flow rate : 5.0mL/minDetector : UV(254nm)Column temp. : 40˚CInjection vol. : 5mLFractionation StandardsFluvalinateAcrinathrincurrent elution fractionformer elution fractionSample :40mg/L eachChinomethionateTricyclazole7Columns for GPC Clean-up0 5 10 15 20 25 30 35 minColumn : Shodex CLNpak EV-G AC + EV-2000 ACEluent : Acetone/Cyclohexane=1/4Flow rate : 5.0mL/minDetector : UV(254nm)Column temp. : 40˚CInjection vol. : 5mL0 5 10 15 20 25 30 35 minColumn : Shodex CLNpak EV-G AC + EV-2000 ACEluent : Acetone/Cyclohexane=1/4Flow rate : 5mL/minDetector : UV(254nm)Column temp. : 40˚CInjection vol. : 5mLElution Positions of Phthalic Acid Estersusing PAE-800 ACRI Corn oil SulferH 2 OCO 2GPC clean-up of Carcinogens in DieselDust measured using PAE-800 ACBenzo[a]fluoranthene1,3-DinitropyreneBisphenol ABenzo[a]pyreneHydroxybenzo[a]pyrene mixturePretreatment of PCBs in Waste OilPCBsRIRIRIRIUV(330nm)UV(330nm)Bis(2-ethylhexyl) adipatePhthalic acid esters4-NonylphenolDDTBisphenol AAnthracene0 15 20 25 30 35minColumn : Shodex CLNpak PAE-800 ACEluent : AcetoneFlow rate : 0.5mL/minDetector : UV(330nm), RIColumn temp. : Room temp.10 20 30minCompound Rt(min) Compound Rt(min)AnthraceneChryseneFluorantheneBenzo[a]flucrantheneBenzo[b]flucrantheneBenzo[k]flucranthenePyrene1-Hydroxypyrene16.618.017.117.718.618.417.919.7Benzo[a]pyreneHydroxybenzo[a]pyrenecompoundBisphenol A1,3-Dinitronaphthalene1-Nitropyrene1,3-Dinitropyrene1,6-Dinitropyrene1,8-Dinitropyrene19.923.016.116.218.618.618.918.7Column : Shodex CLNpak PAE-800 ACEluent : AcetoneFlow rate : 0.8mL/minDetector : UV(210nm)Column temp. : Room temp.Data provided by Prof. Kazuichi Hayakawa,Faculty of Pharmaceutical Sciences, Kanazawa University.Time 14 15 16 17 18 19 20 minVolume 56 60 64 68 72 76 80 mLPCBsChlordane, ToxaphenePolychlorinated naphthalenePolybrominated biphenyletherAliphatic hydrocarbonC 19 C 12 MethylanthraceneAnthraceneColumn : Shodex CLNpak PAE-G AC+ PAE-2000 ACEluent : AcetoneFlow rate : 4.0mL/minDetector : UV(213nm)Data provided by Mr. Koichi Sasaki et al.,Analytical Center, Kokan Keisoku Corporation.

Sample pretreatmentcolumnsColumns for Column Switching methodFeaturesPKGF-4AEffective for both hydrophilic and hydrophobic substancesHigh rate of protein removalHigh rate of protein removal than the PK columnsNewsNo.9, 11, 22* GF-4A column removes proteins well but is not suitable for trapping hydrophilic substances.Use PK columns for this purpose.KW-604SSuitable for the separation of proteins and chemical substances by using SEC mode8Cartridge columns and holder for column switching methodColumns for Column Switching methodProduct CodeF8700000F8700011F8700012F8700013F8700001Product NameMSpak PK-2A 2pMSpak PK-2A 5pMSpak PK-4A 2pMSpak PK-4A 5pMSpak HLDColumns for column switching methodParticle Size(μm)30303030−MaximumPore Size(Å)30303030−Column Size(mm)I.D. x L2.0 x 102.0 x 104.0 x 104.0 x 10(Holder for PK)Shipping SolventH 2 OH 2OH 2 OH 2 O−Pcs/boxBase Material : Hydrophilic copolymers containing N-vinyl acetoamide* PK series are cartridge columns and thus should be installed in acolumn holder "MSpak HLD" before use.25251Product CodeProduct NameBase MaterialParticle Size(μm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping SolventF8700015MSpak GF-4APolyvinyl alcohol94004.6 × 10H 2OF6989106PROTEIN KW-604SSilica71,5006.0 × 50H 2 O*Contact Shodex or our distributors near you for customized columns.

Systems diagram for column switchingPretreatment columnAnalytical columnInjectorDetectorPump 1Pretreatment columns for protein removalColumnPK-2AGF-2A*Company-ARemoval rateof proteins99.62%99.80%97.68%Residual BSA (μg)500400300200100Sample pretreatmentcolumnsEluent 1Proteins etc.in sampleAcetaminophenCaffeineBarbitalPrimidoneTrimethadioneMetharbitalBromvalerylureaEthenzamidePhenobarbitalPhenacetinFlumazenilWarfarinBromazepamChlormezanonePentobarbitalAmobarbitalAcetylpheneturideCarbamazepineClofedanol4-HydroxytriazolamSecobarbital1-HydroxymethyltriazolamMephobarbitalGlutethimideEstazolamIndomethacinAlprazolamPump 2115106949113794949896108938110292939392979191979099931019399Eluent 2Recovery Rates of Medical Compounds using PK-2A columnSampleRecovery(%) SampleOxazepamTriazolamChlordiazepoxidePhenytoinFlurazepamHypaconitineDesipramineEtizolamAconitineBrotizolamNitrazepamMaprotylineNortriptylineBiperidenImipraminePropericiazineTrihexyphenidylClonazepamFlunitrazepamBenzoylaconitineBenzoylhypaconineChlorpheniraminePropranololMesaconineNimetazepamAmitriptylineSildenafil citrate0PK-2A GF-2A Company-A(Pretreatment)Eluent : 10mM Ammonium acetate buffer(pH7.0)Flow rate : 0.5mL/minColumn temp. : Room temp.(Analysis)Column : Shodex PROTEIN KW-604SEluent : 0.1% TFA in (H 2 O/CH 3 CN=50/50)Flow rate : 0.5mL/minDetector : UV(280nm)Column temp. : Room temp.Switching time : 5minRecovery(%) Sample9796133991069710910511097979086999790919697968311197118909395HydroxyzineFludiazepamTrimipramineChlorpromazineHaloperidolDiazepamLevomepromazineBromperidolClomipramineMianserinCloxazolamClotiazepamPromethazineHaloxazolamCarpipraminePerfenazineOxazolamTimiperoneLofepramineZotepineThioridazineMedazepamClocapramineBromocriptineDiphenhydramine*GF-2A: Custom-made column ofGF-4A (2.0mmI.D. x 10mm)Recovery(%)9997107779997968995929196929399869988659297919580939Columns for Column Switching method(Adsorption)Eluent : 10mM Ammonium acetate buffer(pH7.0)Flow rate : 0.5mL/min(Elution)Eluent : 10mM Ammonium acetate buffer(pH7.0)/CH 3 CNFlow rate : 0.5mL/minDetector : UV(220nm)Switching time : 5min

Standard analysiscolumnsPolymer-based Packed Columns forReversed Phase ChromatographyFeaturesODP-50, C8P-50, C4P-50Relative large pore size supports the analysis of proteins, peptides, and amino acidsUseable in a wide pH range from acidic to alkaline (pH 2 to 13)Useable in 100% water or buffer solutionNewsBest to be used for the analysis of basic substancesNo.35 Preparative p.82ColumnsODP-40Higher performance type of ODP-50 seriesNewsNo.1Semi-microMicro Columnsp.70Standard columns10Polymer-based Packed Columns for Reversed Phase ChromatographyProduct CodeF7621001F7621002F7620002F7620001F6710001F6710023F7620004F7620003F6710022F7620009F6713001F7620006F7620005F6710002F7620008F7620007F6710003Asahipak ODP-40 4DAsahipak ODP-40 4EAsahipak ODP-50 6DAsahipak ODP-50 6EAsahipak ODP-50G 6AAsahipak ODP-50 4BAsahipak ODP-50 4DAsahipak ODP-50 4EAsahipak ODP-50G 4AAsahipak ODP-50 2DAsahipak ODP-50G 2AAsahipak C8P-50 4DAsahipak C8P-50 4EAsahipak C8P-50G 4AAsahipak C4P-50 4DAsahipak C4P-50 4EAsahipak C4P-50G 4ASilicapakSILProduct Name≥ 11,000≥ 17,000≥ 9,000≥ 14,000(guard column)≥ 2,500≥ 9,000≥ 14,000(guard column)≥ 5,000(guard column)≥ 7,000≥ 11,000(guard column)≥ 6,000≥ 9,000(guard column)DM-614OctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctylOctylOctylButylButylButylC4P-50C8P-505NH 5CN 5C4 5C8 ODSNH2PPlate Number(TP/column)FunctionalGroupParticle Size(μm)44555555555555555ODP2 HPPore Size(Å)250250250250−250250250−250−250250−250250−ODP-40,50ET-RP1Column Size(mm)I.D. x L4.6 × 1504.6 × 2506.0 × 1506.0 × 2506.0 × 104.6 × 504.6 × 1504.6 × 2504.6 × 102.0 × 1502.0 × 104.6 × 1504.6 × 2504.6 × 104.6 × 1504.6 × 2504.6 × 10RP18-415DS-413,613RP18-413,613DE,GOLF-413,CARB-413Shipping SolventH 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2O/CH 3CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65Base Material : Polyvinyl alcoholFeatures and Applications of Different Packing Materials used for Columnsfor Reversed Phase, Hydrophilic Interaction and Normal phase ChromatographyLargePore size of packing materialsSmallHighSaccharidesSugar alcoholsHydrophilic low molecularweight substancesVitaminsPolycyclicaromaticcompoundsPeptidesProteinsLow molecular weight substances,medicines, organic acids, food additives, etc.Polarity of packing materialsLow*Contact Shodex or our distributors near you for customized columns.

Polymer-based Packed Columns for Reversed Phase ChromatographyFeaturesODP2 HPProvides nearly twice as large theoretical plate number compared with generally availablepolymer-based reversed phase columnsStronger retention of high polar substances compared with ODS columnsSuitable for reversed phase analysis of small molecules such as pharmaceutical compounds in thepresence of protein matrixNoteIdeal for LC/MS analysis of high polar compoundsbook No.6 News No.30, 36Standard analysiscolumnsNEWET-RP1Capable of high temperature analysis up to 150˚CHigh temperature analysis improves column efficiency and enables rapid analysisNewsNo.41RP18-415Large pore size is suitable for the analysis of proteins and peptidesDS-613, 413Suitable for reversed phase analysis of highly hydrophilic substances, which cannot be retainedwell by ODS columns11RP18-613, 413DEDM-614F7622001F7622002F7622003F6714010F7622004F7622005F6714011F7623001F7623003F7009000F7009002F7009001F6709558F7001001F7001012F6700140F7001004F7001005F7009030F7001006F6700150F7001007F6700151F7001002F6700160ODP2 HP-4BODP2 HP-4DODP2 HP-4EODP2 HPG-4AODP2 HP-2BODP2 HP-2DODP2 HPG-2ANEW ET-RP1 4DNEW ET-RP1 3DRSpak RP18-415RSpak RP18-613RSpak RP18-413RSpak RP18-GRSpak DS-613RSpak DS-413RSpak DS-GRSpak DE-613RSpak DE-413RSpak DE-413LRSpak DE-413SRSpak DE-GRSpak DE-213RSpak DE-SGRSpak DM-614RSpak DM-GHigher performance (higher theoretical plates) version of DS-613 and DS-413Polymer-based columns, with similar polarity to that of ODS columns, can be usedin general and wide purposeWide pH range ; useable in 100% water or buffer solutionNews No.14, 23, 24, 35Suitable for the analysis of amino acids and water-soluble vitaminsSee also page 10, "Features and Applications of Different Packing Materials used for Columns for Reversed Phase, Hydrophilic Interaction andNormal phase Chromatography"Standard columnsProduct CodeProduct NamePlate Number(TP/column)≥ 3,500≥ 13,000≥ 17,000(guard column)≥ 3,000≥ 7,000(guard column)≥ 11,000≥ 9,000≥ 5,000≥ 13,000≥ 11,000(guard column)≥ 6,500≥ 11,000(guard column)≥ 7,000≥ 11,000≥ 17,000≥ 3,000(guard column)≥ 8,000(guard column)≥ 4,500(guard column)FunctionalGroup−−−−−−−OctadecylOctadecyl−−−−−−−−−−−−−−−−ParticleBase Material Size(μm)Polyhydroxymethacrylate 5Polyhydroxymethacrylate 5Polyhydroxymethacrylate 5Polyhydroxymethacrylate 5Polyhydroxymethacrylate 5Polyhydroxymethacrylate 5Polyhydroxymethacrylate 5Polyvinyl alcohol 4Polyvinyl alcohol 4Styrene divinylbenzene copolymer 6Styrene divinylbenzene copolymer 3.5Styrene divinylbenzene copolymer 3.5Styrene divinylbenzene copolymer 6Styrene divinylbenzene copolymer 6Styrene divinylbenzene copolymer 3.5Styrene divinylbenzene copolymer 10Polymethacrylate 6Polymethacrylate 4Polymethacrylate 4Polymethacrylate 4Polymethacrylate 10Polymethacrylate 4Polymethacrylate 6Polyhydroxymethacrylate 10Polyhydroxymethacrylate 12Semi-microMicro ColumnsPreparativeColumnsPreparativeColumnsp.70p.82p.82Pore Column SizeSize (mm) Shipping Solvent(Å) I.D. x L404040−4040−250250450200200−200200−25252525−25−200−4.6 × 504.6 × 1504.6 × 2504.6 × 102.0 × 502.0 × 1502.0 × 104.6 × 1503.0 × 1504.6 × 1506.0 × 1504.6 × 1504.6 × 106.0 × 1504.6 × 1504.6 × 106.0 × 1504.6 × 1504.6 × 2504.6 × 504.6 × 102.0 × 1502.0 × 106.0 × 1504.6 × 10H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2O/CH 3CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=5/95H 2 O/CH 3 CN/THF=40/30/30H 2 O/CH 3 CN/THF=40/30/30H 2 O/CH 3 CN/THF=40/30/30H 2 O/CH 3 CN/THF=30/40/30H 2 O/CH 3 CN/THF=40/30/30H 2 O/CH 3 CN/THF=30/40/30H 2OH 2 O/CH 3 CN=50/50H 2 O/CH 3 CN=50/50H 2 O/CH 3 CN=50/50H 2 OH 2 O/CH 3 CN=50/50H 2 O/CH 3 CN=50/505mM H 3 PO 4 aq.5mM H 3 PO 4 aq.Polymer-based Packed Columns for Reversed Phase Chromatography*Contact Shodex or our distributors near you for customized columns.

Polymer-based Packed Columns for Reversed Phase ChromatographyStandard analysiscolumnsFeaturesNNThe packing material contains sulfo groups, and supports multimode (reversed phase and cationexchange) analysisIdeal for analysis of complex samples containing neutral and ionic substancesApplicable for the analysis of organic arsenicsNotebook No.3 News No.7p.70Semi-microMicro ColumnsJJ-50The packing material contains trace amounts of quaternary ammonium groups, and supports multimode(reversed phase and anion exchange) analysisIdeal for analysis of complex samples containing neutral and ionic substancesNews No.712Polymer-based Packed Columns for Reversed Phase ChromatographyGOLF-413CARB-413F7008140F7008150F6700510F7008160F7008240F7008220Columns for pesticides analysis such as asulam and oxine-CuSupports a wide pH rangeSuitable for the analysis of metal coordination substancesColumns for N-methylcarbamate pesticides analysisSee also page 10, "Features and Applications of Different Packing Materials used for Columns for Reversed Phase, Hydrophilic Interaction andNormal phase Chromatography"Standard columnsProduct CodeFor pesticidesProduct CodeProduct NameRSpak NN-814RSpak NN-614RSpak NN-GRSpak NN-414RSpak JJ-50 4DRSpak JJ-50 2DProduct NamePlate Number(TP/column)≥ 9,000≥ 4,000(guard column)≥ 6,000≥ 4,500≥ 3,500FunctionalGroupSulfoSulfoSulfoSulfoQuaternaryammoniumQuaternaryammoniumApplicationBase MaterialPolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylatePolyhydroxymethacrylatePolyvinyl alcoholPolyvinyl alcoholParticleSize(μm)1010101055FunctionalGroupPoreSize(Å)200200−200100100Column Size(mm)Shipping SolventI.D. x L0.1M Sodium phosphate buffer8.0 × 250(pH3.0)0.1M Sodium phosphate buffer6.0 × 150(pH3.0)0.1M Sodium phosphate buffer6.0 × 50(pH3.0)0.1M Sodium phosphate buffer4.6 × 150(pH3.0)4.6 × 150 H 2 O/CH 3 CN=40/602.0 × 150Column Size(mm)I.D. x LH 2 O/CH 3 CN=40/60Shipping SolventF7009010RSpak GOLF-413Asulam, Oxine-Cu, etc−4.6 × 150H 2O/CH 3CN=50/50F7009040RSpak CARB-413N-methylcarbamate pesticides−4.6 × 150H 2 O/CH 3 CN=50/50F7009041RSpak CARB-413LN-methylcarbamate pesticides−4.6 × 250H 2 O/CH 3 CN=50/50F6700152RSpak CARB-LF(Line Filter for CARB-413, 413L)−6.0 × 10H 2 O/CH 3 CN=50/50F6700150RSpak DE-G(guard column)−4.6 × 10H 2 O/CH 3 CN=50/50Base Material : Polymethacrylate*Contact Shodex or our distributors near you for customized columns.

Polymer-based Packed columns forHydrophilic Interaction Chromatography (HILIC)FeaturesNH2P-50Suitable for saccharides analysis by hydrophilic interaction chromatography (HILIC)Polymer-based packing material provides excellent chemical stability and resistance todegradation over timeWashable with alkaline solutionAlso suitable for evaporative light-scattering detector, corona-charged aerosol detector,and LC/MSNotebook No.2 News No.6, 14, 17, 32PreparativeColumnsp.83Standard analysiscolumnsNEWNH2P-40Higher performance of the NH2P-50 seriesPerforms high resolution and enables solvent savings with a conventional equipmentNewsNo.44Semi-microMicro Columnsp.7013See also page 10, "Features and Applications of Different Packing Materials used for Columns for Reversed Phase, Hydrophilic Interaction andNormal phase Chromatography"Standard columnsProduct CodeF7630007F6710030F7630002F7630001F6710016F7630006F6713000F6710100NEWNEWProduct NameAsahipak NH2P-40 3EAsahipak NH2P-50G 3AAsahipak NH2P-50 4DAsahipak NH2P-50 4EAsahipak NH2P-50G 4AAsahipak NH2P-50 2DAsahipak NH2P-50G 2AAsahipak NH2P-LFPlate Number(TP/column)≥ 8,500(guard column)≥ 5,500≥ 7,500(guard column)≥ 3,500(guard column)(line filter)FunctionalGroupAminoAminoAminoAminoAminoAminoAminoAminoParticle Size(μm)4555555−Pore Size(Å)100−100100−100−−Column Size(mm)I.D. x L3.0 × 2503.0 × 104.6 × 1504.6 × 2504.6 × 102.0 × 1502.0 × 108.0 × 75Shipping SolventH 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2O/CH 3CN=25/75H 2 O/CH 3 CN=25/75Base Material : Polyvinyl alcoholPolymer-based Packed columns for Hydrophilic Interaction Chromatography (HILIC)*Contact Shodex or our distributors near you for customized columns.

Standard analysiscolumnsEffect of salt concentration in the analysis of basic substance25mM10mMODP-500 100 10ODS-A0 10ODS-B0 100 10Sample : Scopolamine 1.0mg/mL, 50μLWhen using an ODS column, nonspecificadsorption of samples is observed underlow ionic concentration conditions, andcomplete adsorption without any elutionis achieved under the absence of coexistingions.However, when using ODP-50, apolymer-based column, the sharp peakof scopolamine is observed regardlessof the ionic concentration, even underthe absence of coexisting ions, suggestingthat the problematic nonspecific adsorptionhas been eliminated.AlkylalcoholsC8C6C4EGC6C10C12C14C8C4EG C10 C12C4P-50C8P-50C14Sample : Ethyleneglycol, n-Alkylalcoholelution volume (mL)ODP-501510C8P-505C4P-502 4 6 8 10 12 14carbon number (n)14Polymer-based Packed Columns for Reversed Phase Chromatography1mM0mM0 100 min 10Local anesthetics231pH3 pH7 pH110 20 min213adsorption10 0 100 min min54670 101230 20 minColumn : Shodex AsahipakODP-50 4DEluent : 25mM Phosphate buffer/CH 3 CN=60/40Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚CSample : 20μL1. Vitamin K3 1.5μg/mL2. Vitamin A 1.0 IU/mL3. Vitamin A acetate 0.5 IU/mL4. Vitamin D2 13.2μg/mL5. Vitamin D3 13.2 IU/mL6. Vitamin E acetate 2.4μg/mL7. Vitamin E 2.5μg/mL8. Vitamin K1 2.4μg/mLadsorption8Column : Shodex AsahipakODP-50 4DODS-A (company-A)ODS-B (company-B)Eluent : Phosphate buffer(pH7.0)/CH 3 CN=60/40Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚C012320 minSample :C6 C4EG0 5Dissociation of tertiary amino groups contained in basic chemical substances can be suppressed by makingthe pH of the eluent higher than the pKa of the amino groups.This increases the relative hydrophobicity of the chemical substances, thereby allowing the column to holdthe chemical substances stronger to separate them more clearly.1. BenzocaineH2N2. LidocaineCH3CH33. TetracaineNHCOCH2NCOOCH2CH2NNH(CH2)3CH31C8C1010 15 20 25 minCOOC2H5C2H5C2H5CH3CH3Sample : Licorice1. GlycyrrhizinODP-50C12020%CH3CNRelationship between the Number of CarbonAtoms of Alkylalcohols and Elution Volume1C14Macrolide antibiotics15 10 15 20 25min2 3456789Column : Shodex AsahipakODP-50 4DC8P-50 4DC4P-50 4DEluent : H 2 O/CH 3 OH=20/80Flow rate : 0.6mL/minDetector : RIColumn temp. : 30˚C101112Sample : 0.1% each, 10μL1. Erythromycin2. AzithromycinColumn : Shodex Asahipak ODP-40 4EEluent : 40mM Potassium phosphate buffer(pH11.0)/CH 3 CN=40/60Flow rate : 0.5mL/minDetector : UV(223nm)Column temp. : 40˚CFat-soluble vitamins Glycyrrhizin in licorice powders Gradient analysis of proteins and peptides2Sample : 40μL12345678910111250%CH3CNSample Mw Recovery(%)Lys-Bradykinin 1188 97Bradykinin 1060 92Met-Enkephalin 574 97Neurotensin 1673 99Leu-Enkephalin 556 100Substance P 1348 93Bacitracin 1450 81Insulin5750 95Insulin B chain 3496 91Lysozyme 14300 96Mastoparan 1479 96Myoglobin 17500 830 5 10 15 20 25minColumn : Shodex Asahipak ODP-50 4EEluent : CH 3 CN/CH 3 OH=50/50Flow rate : 0.6mL/minDetector : UV(280nm)Column temp. : 30˚C0 10 minColumn : Shodex Asahipak ODP-50 6DEluent : 0.05% TFA aq./CH 3 CN=62/38Flow rate : 1.0mL/minDetector : UV(248nm)Column temp. : 30˚C0 10 20 minColumn : Shodex Asahipak ODP-50 6DEluent : (A); 0.05% TFA aq./CH 3CN=80/20(B); 0.05% TFA aq./CH 3 CN=50/50Linear gradient; (A) to (B), 20minFlow rate : 1.0mL/minDetector : UV(220nm)Column temp. : 30˚C

Comparison between ODP2 HP and ODP-50 (existing)ODP2 HP-4D123N:11,4004ODP-50 4D (existing)1Sample : 5μL1. Phenol 300mg/L2. Methyl benzoate 350mg/L3. Toluene 1000mg/L4. Naphthalene 150mg/L23N:5,7004Imidazoles12Sample : 0.1% each, 10μL1. Imidazole2. 2-Methylimidazole3. 4-Methylimidazole3Standard analysiscolumns0 5 10min0 5 10 0510minminColumn : Shodex ODP2 HP-4DEluent : H 2 O/CH 3 CN=55/45Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 40˚CIntensityIntensity120000110000100000900008000070000600005000040000300002000010000012000011000010000090000800007000060000500004000030000200001000001000000ODP2 HPBarbital 500ng/mLODS 1st8000001100001000006000009000080000700004000006000050000Barbital40000Barbital200000Peak Area : 100%30000Peak Area : 100%200000100000BarbitalPeak Area : 99%0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5Time (min)IntensityIntensityColumn : Shodex AsahipakODP-50 4DEluent : H 2 O/CH 3 CN=35/65Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 40˚CComparison of recovery rates of the barbital in BSA betweenODP2 HP-2B and ODSFor the LC/MS analysis of chemical substances containing proteins or other similarsamples, it is generally said that an ODS column is susceptible to ion suppressiondue to the presence of proteins. However, ODP2 HP does not hold proteins andelutes them at one time, and thus is resistant to the effects of such ion suppression.Barbital 500ng/mLBarbital 500ng/mL in BSA 7.0mg/mLBarbital 500ng/mL in BSA 7.0mg/mL1100001000009000080000700006000050000400003000020000100000Column : Shodex ODP2 HP-2B, ODS (company-A)Eluent : 10mM Ammonium acetate aq./CH 3 CN=70/30Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative : m/z 183)Column temp. : 30˚CInjection vol. : 10μLBarbitalPeak Area : 71%0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5Time (min)Column : Shodex ODP2 HP-4EEluent : 10mM Na 2 HPO 4 aq./CH 3 CN=90/10Flow rate : 0.8mL/minDetector : UV(220nm)Column temp. : 40˚CIntensityIntensity1000000Direct analysis of chemical substances in biofluids without pretreatmentRepeated results of LC/MS analysisAn experiment repeated 150 times for chemical substance analysis in the presenceof protein (BSA) revealed that ODP2 HP elutes protein without retaining it and henceposes no significant problem in terms of repeatability.800000600000400000200000150th00 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18Time (min)Sample :500ng/mL each in 0.7mg/mL BSA, 5μL1. Lidocaine2. ImipramineColumn : Shodex ODP2 HP-2BEluent : 10mM Ammonium acetate aq./CH 3 CN=70/30Flow rate : 0.2mL/minDetector : ESI-MSColumn temp. : 40˚C[Direct analysis of serum acetaminophen] [Direct analysis of serum dihydrocodeine phosphate] [Direct analysis of risperidone in urine]15Polymer-based Packed Columns for Reversed Phase ChromatographyAcetaminophen(20.7μg/mL)21.10minSample :Human serum, 10μLAcetaminophen2-Acetaminophenol2-Acetaminophenol (internal standard)25.2minSample : Human serum, 10μLDihydrocodeine phosphate(74.6μg/mL)29.9minSample : 10μLHuman Urine (5 fold diluted)RisperidoneRisperidone(6.4μg/mL)38.0min0 10 20 30 40 min0 10 20 30 min 0 10 20 30 40 minColumn : Shodex ODP2 HP-4DEluent : 0.1% TFA aq./CH 3 CN=93/7Flow rate : 0.5mL/minDetector : UV(254nm)Column temp. : 40˚CColumn : Shodex ODP2 HP-4DEluent : 0.1% TFA aq./CH 3 CN=93/7Flow rate : 0.3mL/minDetector : UV(230nm)Column temp. : 40˚CColumn : Shodex ODP2 HP-4DEluent : 0.1% TFA aq./CH 3 CN=93/7Flow rate : 0.6mL/minDetector : UV(215nm)Column temp. : 40˚CData provided by Dr. Katsuko Hara and Dr. Yutaka Komiyama of the Department of Clinical Laboratory, Kansai Medical University Takii Hospital

Standard analysiscolumnsComparison of the ET-RP1 column’s efficiency (theoretical plate height) between highand normal temperatureshigh temp. (150˚C)2.4mL/min2 3normal temp. (40˚C)0.5mL/min23Reduced plate height *SampleAcetophenoneBenzene40˚C3.23.6150˚C2.42.3* Plate height / particle diameter of the packed resinSample :1. Uracil2. Pyridine3. Acetophenone4. Benzene4Rapid analysis of artificial colorants athigh temperatures12Sample : 45μg/mL each, 20μL1. Food red No.22. Food red No.1023. Azorubine4. Patent blue V1 41340 1 2 3 min 02 4 6 8 10 12 14 min0 5 10 min16Polymer-based Packed Columns for Reversed Phase ChromatographyColumn : Shodex ET-RP1 4DEluent : (Left) H 2 O/CH 3 CN=50/50(Right) H 2O/CH 3CN=75/25Detector : Diode array(210nm)Column Oven : Polaratherm 9000 Series(SandraSelerity Technonogies, Inc)Separation and recovery rates of standardproteins12340 10 20 30 min213556Recovery of proteins (%)1. Ribonuclease A 932. Insulin983. Cytochrome c 1004. Lysozyme 1005. BSA986. Myoglobin 1087. Ovalbumin −Column : Shodex RSpak RP18-415Eluent : (A); 0.1% TFA aq./CH 3 CN=99/1(B); 0.1% TFA aq./CH 3 CN=5/95Linear gradient; 20%(B) to 60%(B), 20minFlow rate : 1.0mL/minDetector : UV(220nm)Column temp. : Room temp.Organic acids7Sample : 5μL1. Glyoxylic acid 1.78mg/mL2. Tartaric acid 1.95mg/mL3. Malic acid 2.06mg/mL4. Lactic acid 2μL/mL5. Malonic acid 1.95mg/mL6. Acetic acid 2μL/mL7. Succinic acid 2.05mg/mL8. Levulinic acid 1.95mg/mL9. Propionic acid 2μL/mLNote :The eluent was introduced into thecolumn after being preheated andwas cooled after column elution,then introduced into the detector.Data provided by Research Institute for Chromatography bvbaAlkylbenzenes2134560 2 4 6 8 10 min0 5 10 min1Sample : 5μL1. m-Cresol 0.1%2. 2,4-Xylenol 0.1%3. Benzene 0.5%4. Toluene 0.5%5. Ethylbenzene 0.5%6. n-Propylbenzene 0.5%Column : Shodex RSpak DS-613Eluent : H 2 O/CH 3 CN/THF=30/40/30Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 40˚CFood additives (Preservatives)Sample : 10μL1. Saccharin sodium 0.005%2. p-Hydroxybenzoic acid 0.005%3. Sorbic acid 0.02%4. Benzoic acid 0.02%5. Methyl paraben 0.01%6. Dehydroacetic acid 0.01%7. Ethyl paraben 0.02%8. Propyl paraben 0.02%7Column : Shodex ET-RP1 3DEluent : 6mM Tetrabutylammonium bromide in(25mM Ammonium acetate aq./CH 3CN=70/30)Flow rate : 0.5mL/minDetector : UV(254nm)Column temp. : 70˚CFatty acid methyl estersDiolsn=234512Sample : 0.2% each, 20μL1. Methyl linoleate2. Methyl palmitate3. Methyl oleate4. Methyl stearateColumn : Shodex RSpak DS-413Eluent : H 2 O/CH 3 CN/THF=25/45/30Flow rate : 1.0mL/minDetetor : RIColumn temp. : 40˚C34Sample : 1% each, 7.5μLHO(CH2)nOH4678923456860 1 2 3 4 5 6 7 8 minColumn : Shodex RSpak DE-413Eluent : 10mM Phosphoric acid aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 50˚C0 5 10 15 20 min 0 5 10 15 minColumn : Shodex RSpak DE-413Column : Shodex RSpak DE-613Eluent : (50mM KH 2 PO 4 + 0.1% H 3 PO 4 ) aq. Eluent : H 2 O/CH 3 CN=65/35Flow rate : 1.0mL/minFlow rate : 1.0mL/minDetector : RIDetector : UV(210nm)Column temp. : 60˚CColumn temp. : 40˚C

Hydantoins3214RONONHSample :Hydantoins, 0.05% each, 3μL1. 5-Carboxyethyl hydantoin2. 5-Hydroxymethyl hydantoin3. Hydantoin4. 5-Methyl hydantoin5. 5-Ethyl hydantoinCholine and acetylcholine13Sample : 10μL1. Choline 5mg/L2. Ethylhomocholine 10mg/L3. Acetylcholine 10mg/LDegasPumpDE-413ACH-494ECDStandard analysiscolumnsColumn oven5Eluent0 2 4 6 8 minColumn : Shodex RSpak DE-613Eluent : 1/30M Na 2 HPO 4 + 1/30M KH 2 PO 4 aq.Flow rate : 2.0mL/minDetector : UV(210nm)Column temp. : 50˚CAnalysis of diarrheal and fat-soluble shellfish poisoningOkadaic acidSIM : 803.5PTX2(Pectenotoxin-2)SIM : 903.5DTX3(Dinophysistoxin-3)SIM : 1055.7N-methylcarbamate pesticides1234InjectorPumpDegasserEluentDE-G5CARB-4136PumpNaOH aq.reagent790˚CReaction coilPumpOPAreagentreagent8PTX6(Pectenotoxin-6)SIM : 887.5DTX1(Dinophysistoxin-1)SIM : 817.5PTX1(Pectenotoxin-1)SIM : 919.540˚CReaction coilFluorescencedetectorSample : 0.1ppm each, 20μL1. Oxamyl2. Methiocarb sulfoxide3. Aldicarb4. Methiocarb sulfone5. Bendiocarb6. Ethiofencarb7. Carbaryl8. Fenobucarb9. Methiocarb920 510 minMorphology of arsenic compounds200010002000digestive cecum of shellfish,1g90% Methanol, 9mLhomogenize (3-5 min)inject into LCMS 5uL ofsupernatant0YTX(Yessotoxin)SIM : 1141.5Sample pretreatmentcentrifuge (3,000rpm, 5min)Standard arsenic compounds12 3 425 6100 200 300 400 500 600 700 800 900 10001100 1200Urine from an arsenicpoisoning patientColumn : Shodex RSpak DE-413Post column : Shodex AFpak ACH-494Eluent : 0.1M Phosphoric acid +300mg/L Sodium 1-decansulfonate +65mg/L Tetramethylammonium chloride(pH8.0 adjusted by 1M NaOH)Flow rate : 1.0mL/minDetector : EC(Electrode: Pt, 350mV SCE)Column temp. : 37˚CColumn : Shodex DE413-2BEluent : (A); 50mM HCOOH + 2mM HCOONH 4 aq.(B); 50mM HCOOH + 2mM HCOONH 4 in(H 2 O/CH 3 CN=5/95)0 to 5min, 20% (B)5 to 12min, 20% (B) to 100% (B)Flow rate : 0.2mL/minDetector : ESI-MS(Negative)2000* See p.28 for ACH-494While a toxicity testing using mice is a mainstreammethod for examination of shellfish poisoning, ananalytical method using LC/MS was developed tomeet the efficient and high-performance shellfishpoisoning examination to cope with the longinspection time and variance in laboratory resultsof the conventional inspection method, and toreduce the use of animals. The use of LC/MSenabled rapid detection of components which aredifficult to be detected in an animal study.Data provided by Mr. Toshiyuki Suzuki of the Fisheries Research AgencySample : Arsenic compounds, 50μL1. Monomethylarsonic acid2. Arsinic acid3. Dimethylarsinic acid4. Arsenobetaine5. Tetramethylarsonium6. Trimethylarsine oxideUrine of a healthy person417Polymer-based Packed Columns for Reversed Phase Chromatography1000410000 10 20 30 minColumn : Shodex RSpak CARB-413Eluent : CH 3 CN/H 2 O=37/63Flow rate : 0.8mL/minReagent : (A); 50mM NaOH(B); 0.05% o-Phthalaldehyde + 0.1% MercaptoethanolDetector : Fluorescence(Ex. : 340nm, Em. : 450nm)Column temp. : 40˚C130 100 200 300 400 500 600 700 800 900 1000 1100 1200Column : Shodex RSpak NN-614Eluent : 5mM HNO 3 /8mM NH 4 NO 3 aq.Flow rate : 0.8mL/minDetector : IC-ICP-MS(SIM m/z=75)1235 6100 200 300 400 500 600 700 800 900 100011001200Noriko TSUNODA,National Reseach Institute of Police Science,Pharmacia, vol.34 No.12 (1998), p1237-12421

Standard analysiscolumnsAmino acid analysis using multimodeSample : 20μL1. Aspartic acid2. Glycine3. Alanine4. Valine5. Methionine6. Isoleucine32Haloacetic acid analysis using multimodeSample : 500mg/L each, 2μL1. Chloroacetic acid2. Bromoacetic acid3. Dichloroacetic acid4. Dibromoacetic acid5. Trichloroacetic acid4Phenylenediamine isomers analysis usingmultimodeSample : 100mg/L each, 20μL1. p-Phenylenediamine2. m-Phenylenediamine3. o-Phenylenediamine121423563510 20 40 60 80 min0 5 10 15 20 min0 5 10 15 min18Polymer-based Packed Columns for Reversed Phase ChromatographyPolymer-based Packed columns for Hydrophilic Interaction Chromatography (HILIC)Column : Shodex RSpak NN-814Eluent : 40mM Phosphoric acid aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CAscorbic acid and erythorbic acidSample : 5μg/mL each, 10μg1. Erythorbic acid 2. L-Ascorbic acidO=CO=CHOCHOCOOHOCHOCHCHCHCOH1 HOCHCH2OHCH2OH2600000400000200000215001000Column : Shodex RSpak JJ-50 2DEluent : 25mM Ammonium acetate buffer(pH9.2)/CH 3 CN=50/50Flow rate : 0.2mL/minDetector : UV(210nm)Column temp. : 40˚CErythorbic acidR 2 =0.99990 200 400Erythorbic acidL - Ascorbic acidR 2 =0.9997NH2P-50 4EFirst use13214Sucrose14Sucrose5001212L - Ascorbic acid10Glucose10Glucose8Fructose8Fructose00 5 10 min0 0.5 150 100 150 20050 100 150 200Sample concentration(μg/mL)Time (hr)Time (hr)Column : Shodex Asahipak NH2P-50 4EColumn : Shodex Asahipak NH2P-50 4E, Silica based amino column (company-A)Eluent : 20mM NaH 2 PO 4 + 30mM H 3 PO 4 (pH2.2)/CH 3 CN=20/80Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minFlow rate : 1.0mL/minDetector : UV(254nm)Detector : RIColumn temp. : 30˚CColumn temp. : 30˚CComparison between NH2P-40 3E and NH2P-50 4ENH2P-40 3E (New)(3.0mmI.D. x 250mm)Peak height(μV)Peak height(μV)Sample concentration(μg/mL)NH2P-50 4E (existing)(4.6mmI.D. x 250mm)0 1020181641. Fructose2. Glucose3. Sucrose4. MaltoseColumn : Shodex RSpak JJ-50 4DEluent : 25mM Ammonium acetate buffer(pH9.2)/CH 3 CN=70/30Flow rate : 0.4mL/minDetector : UV(254nm)Column temp. : 30˚CRepeated analysis with NH2P-50 column and silica-based amino columnFor every kind of saccharide, the resistance to time degradation of our polymer-basedamino column NH2P, enables to maintain a stable retention time and obtaining sharppeaks.Ve (mL)After 180hrs1321. Fructose12. Glucose3. Sucrose 2 34 4. Maltose420 min 0 10 20 min 0 10 20 min 0 10 20 minMaltoseSample : 0.5% each, 4μL1. Fructose2. Glucose3. Sucrose4Silica-based amino columnFirst use1Ve (mL)20181632MaltoseAfter 120hrsThe NH2P-40 3E is a polymer-based amino column (3 mm inner diameter)packed by downsizing particle size. This general-purpose columnperform higher resolution and separation of the conventional column(NH2P-50 4E).1TPN (Sucrose)Fructose/GlucoseRsGlucose/SucroseNH2P-40 3E11,3006.48.5NH2P-50 4E8,7004.25.92312305 10 15 20 0 5 10minmin15 20Column : Shodex Asahipak NH2P-40 3E, NH2P-50 4EEluent : H 2 O/CH 3 CN=25/75Flow rate : (NH2P-40 3E) 0.35mL/min, (NH2P-50 4E) 1.0mL/minDetector : RIColumn temp. : 25˚C

0Saccharides analysis using corona-charged aerosol detector0 5112Silica-type amide column(4.6mmI.D. x 150nm)121Silica-type amino column(4.6mmI.D. x 250nm)5 10 15 min23Sample : 40μg/mL each, 5μL1. Fructose2. Glucose4526NH2P-50 4E(4.6mmI.D. x 250nm)Column : Shodex Asahipak NH2P-50 4ESilica based amino column (company-A),Silica based amide column (company-B)Eluent : H 2 O/CH 3 CN/=20/80Flow rate : 1.0mL/minDetector : Corona charged aerosolColumn temp : 30˚C(Amino column), 80˚C(Amide column)Fructooligosaccharide syrupSample :Fructooligosaccharide syrup, 2.5%, 20μL1. Fructose2. Glucose3. Sucrose4. 1-Kestose5. Nystose6. 1-Fructofuranosyl-D-nystose10 15 20 25 minColumn : Shodex Asahipak NH2P-50 4EEluent : CH 3 CN/H 2 O=70/30Flow rate : 1.0mL/minDetector : RIColumn temp. : 25˚CLC/TOF-MS analysis of 2-amino benzoic acid-derivatizedsugar chains123A corona-charged aerosoldetector measures effluentsas particles. Accordingly, thebaseline will be significantlyinfluenced by componentseluted from the column. Thepolymer-based amino column,NH2P, elutes extremely smallamounts of components, andthus enables a stable baselinewith lower noise level to beestablished.Oligogalacturonic acids1234Sample : Oligogalacturonic acids1. Galacturonic acid2. Oligogalacturonic acid dimer3. Oligogalacturonic acid trimer4. Oligogalacturonic acid tetramer5. Oligogalacturonic acid pentamer6. Oligogalacturonic acid hexamer56DP 1 2 3 4 5 6 70 1 2 3 4 5 6 7 8 9 10Retention time (min)ChitooligosaccharidesSample : Chitooligosaccharides 2%, 20μL1. D-Glucosamine2. Chitobiose hydrochloride3. Chitotriose hydrochloride4. Chitotetraose hydrochloride5. Chitopentaose hydrochloride6. Chitohexaose hydrochloride0 5 10 15 20 min 0 5 10 15 minColumn : Shodex Asahipak NH2P-50 4EEluent : 0.3M Sodium phosphate buffer(pH4.4)Flow rate : 1.0mL/minDetector : UV(210nm)Column temp. : 40˚CSample offered by Prof. Yoshino in Kyoto Women's Univ.Sample : 2μLHuman IgG (1μg protein)2AA-labeled N-Glycan* 2AA : 2-aminobenzoic acidProducts for cellulose degradation by cellulase (PcCel45A)treatmentColumn : Shodex Asahipak NH2P-50 4EEluent : (A);H 2 O/CH 3 CN=40/60(B);H 2 O/CH 3 CN=50/50Linear gradient; (A) to (B),10minFlow rate : 1.0mL/minDetector : Corona charged aerosolColumn temp : 40˚CData provided by Kiyohiko Igarashi, Professor of the Laboratory of Forest Chemistry,Biomass Chemistry Course, Department of Biomaterial Sciences,Graduate School of Agricultural and Life Sciences, University of TokyoPyridylaminated monosaccharides123123Column : Shodex Asahipak NH2P-50 4EEluent : CH 3 CN/H 2 O=70/30Flow rate : 1.0mL/minDetector : RIColumn temp. : 25˚C4 5 6544Sample : 5μL1. 2-Aminopyridine 0.1pmol/μL2. PA-Rhamnose 1pmol/μL3. PA-Fucose 1pmol/μL4. PA-Ribose 1pmol/μL5. PA-Xylose 1pmol/μL6. PA-N-Acetylglucosamine 1pmol/μL7. PA-N-Acetylgalactosamine 1pmol/μL8. PA-Mannose 1pmol/μL9. PA-Glucose 1pmol/μL10. PA-Galactose 1pmol/μL56Standard analysiscolumns19Polymer-based Packed columns for Hydrophilic Interaction Chromatography (HILIC)6789105 10Migration time (min)15 20Column : Shodex NH2P40-2DEluent : A; 95% CH 3 CN/0.1% Formic acidB; 5% CH 3 CN/0.1% Formic acidGradient : Linear gradient: B%, 30%(0-2.5min), 30-95%(2.5-20min)Flow rate : 0.2mL/minDetector : ESI-TOF MS(Polarity : Negative, Full MS range : 2000 )Column oven : 45˚CData provided by Mr. Michihiro Kinoshita, Faculty of Pharmacy, Kinki University020 40 60 minColumn : Shodex Asahipak NH2P-50 4EEluent : H 3PO 4/H 2O/CH 3CN=1/14/85Flow rate : 0.5mL/minDetector : Fluorescence(Ex. : 310nm, Em. : 380nm)Column temp. : 40˚C

Standard analysiscolumnsSilica-based Packed Columns for Reversed Phase,HILIC and Normal Phase Chromatography[ODS columns]FFeaturesC18MThis ODS column remains for over 30 years as the selling productMonomeric type ODS column, fully end-capped high-purity silica(99.99% or higher)PreparativeColumnsp.83C18PPolymeric type ODS column, fully end-capped high-purity silica (99.99% or higher)Excellent acid resistanceAdvantageous for separating planar and nonplanar compounds from each other20Silica-based Packed Columns for Reversed Phase, HILIC and Normal Phase ChromatographySee also page 10, "Features and Applications of Different Packing Materials used for Columns for Reversed Phase, Hydrophilic Interaction andNormal phase Chromatography"Standard columnsProduct CodeF6604112F6605110F6604113F6650040F6650041F6650042F6650045F6650046F6650047F6604110F6650050F6650051F6650052F6650053F6650054F6650055F6650058F6650059F6650062F6650063F6650060F6650061ODSpak F-411ODSpak F-511ODSpak F-411/SSilica C18M 4DSilica C18M 4ESilica C18M 2DSilica C18P 4DSilica C18P 4ESilica C18P 2DSilicapak E-411Silica 5SIL 4DSilica 5SIL 4ESilica 5C8 4DSilica 5C8 4ESilica 5C4 4DSilica 5C4 4ESilica 5CN 4DSilica 5CN 4ESilica 5NPE 4DSilica 5PYE 4DSilica 5NH 4DSilica 5NH 4EPlate Number(TP/column)≥ 8,000≥ 14,000≥ 10,000≥ 10,000≥ 16,000≥ 9,000≥ 10,000≥ 16,000≥ 9,000[Other Silica-based columns]FeaturesE-4115SIL5C85C45CN5NPE,5PYE5NHPlate Number(TP/column)≥ 8,000≥ 9,000≥ 15,000≥ 9,000≥ 15,000≥ 9,000≥ 15,000≥ 7,000≥ 12,000≥ 8,000≥ 7,000≥ 5,000≥ 8,000FunctionalGroupOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylParticle Size(μm)553555553Particle Size(μm)5555555555555*Contact Shodex or our distributors near you for customized columns.141414161616171717−−−101077−−−−−−Pore Size(Å)100100100100100100100100100Pore Size(Å)50100100100100100100100100100100100100Column Size(mm) I.D. x L4.6 × 1504.6 × 2504.6 × 1004.6 × 1504.6 × 2502.0 × 1504.6 × 1504.6 × 2502.0 × 150Column Size(mm) I.D. x L4.6 × 1504.6 × 1504.6 × 2504.6 × 1504.6 × 2504.6 × 1504.6 × 2504.6 × 1504.6 × 2504.6 × 1504.6 × 1504.6 × 1504.6 × 250H 2 O/CH 3 OH=20/80H 2 O/CH 3 OH=20/80H 2 O/CH 3 OH=20/80H 2 O/CH 3 OH=30/70H 2 O/CH 3 OH=30/70H 2 O/CH 3 OH=40/60H 2 O/CH 3 OH=30/70H 2 O/CH 3 OH=30/70H 2 O/CH 3 OH=40/60Base Material : SilicaSee also page 10, "Features and Applications of Different Packing Materials used for Columns for Reversed Phase, Hydrophilic Interaction andNormal phase Chromatography"Standard columnsProduct CodeProduct NameSilica gel packed column without any chemical modificationProduct NameFunctionalGroupCarbon Load(%)Uses high-purity silica (99.99% or higher)Like E-411, suitable for normal phase analysis using a nonpolar organic solventTo use when the retention capacity of C18 or C8 is excessively highSuitable for saccharides analysis by hydrophilic interaction chromatography (HILIC)Carbon Load(%)Shipping SolventTo use when the retention capacity of C18 is excessively higher or that of 5C4 is excessively lowerApplicable to ion-pair chromatography because of its rapid mass transfer and achievementof equilibriumPreparativeColumnsPreparativeColumnsUtilizes reversed phase interaction and π-electron interaction to separate regioisomers, which typicallycannot be separated with ODS, C8, or C4 columnsUtilizes several types of interactions based on π-electrons to separate structural isomers−−−OctylOctylButylButylCyanopropylCyanopropylNitrophenylethylPyrenylethylAminopropylAminopropylPreparativeColumnsPreparativeColumnsShipping Solventp.83p.83p.83p.83IPA/Dichloromethane/n-Hexane=1/50/49C 6 H 14 /C 2 H 5 OH=95/5C 6 H 14 /C 2 H 5 OH=95/5H 2 O/CH 3 OH=34/66H 2 O/CH 3 OH=34/66H 2 O/CH 3 OH=45/55H 2 O/CH 3 OH=45/55H 2 O/CH 3 OH=60/40H 2 O/CH 3 OH=60/40H 2 O/CH 3 OH=45/55H 2 O/CH 3 OH=30/70H 2 O/CH 3 CN=5/95H 2 O/CH 3 CN=5/95Base Material : Silica

Hippuric acids in urineStandards123Sample : 10μL1. Mandelic acid 17μg/mL2. Hippuric acid 17μg/mL3. o-Methylhippuric acid 17μg/mL4. p-Methylhippuric acid 17μg/mL5. m-Methylhippuric acid 17μg/mL6. Creatinine 33μg/mL4562Urine(10-fold diluted)6AflatoxinsReference : Shoku-An No. 0728001 (July 28, 2006, Japan)Test method of Aflatoxin B1 in cereals, puises, nuts andseeds, spices "multifunctional column"1234TFA methodSample :5μg/L each, 20μL1. Derived Aflatoxin G12. Derived Aflatoxin B 13. Aflatoxin G24. Aflatoxin B 2Standard analysiscolumns00510 min05Column : Shodex ODSpak F-411Eluent : [20mM Phosphoric acid + 20mM β-Cyclodextin(pH2.5)]/CH 3 CN=88/12+ 4.5mM Sodium dodecyl sulfateFlow rate : 1.0mL/minDetector : UV(225nm)Column temp. : 50˚CTrichothecene mycotoxinReference : Shoku-An No. 0717001 (July 17, 2003, Japan)Test method of deoxynivalenol1Sample : 20μL1. Nivalenol (INIV)2. Deoxynivalenol (DON)5 10 15 minColumn : Shodex Silica C18M 4EEluent : H 2 O/CH 3 CN/CH 3 OH=90/5/5Flow rate : 1.0mL/minDetector : UV(220nm)Column temp. : 40˚CBenzylpyridine isomers1* 1 ppm each of DON andNIV were added to wheatsample22Sample :1. 2-Benzylpyridine2. 3-Benzylpyridine3. 4-Benzylpyridine3Isoflavones12132310 min0 5 10 15 minDinitronaphthalene isomers4Sample :1. Daidzin 1.0μg2. Genistin 0.5μg3. Daidzein 1.0μg4. Genistein 1.0μgColumn : Shodex Silica C18P 4DEluent : 0.1% Acetic acid aq./CH 3 OH=70/30Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 30˚CSample :1. Naphtalene2. 1,5-Dimethylnaphtalene3. 1,5-Dinitronaphtalene4. 1,8-Dinitronaphtalene40 5 10 15 20 minColumn : Shodex Silica C18M 4EEluent : H 2 O/CH 3 CN/CH 3 OH/=60/10/30Flow rate : 1.0mL/minDetector : Fluorescence(Ex. : 365nm, Em. : 450nm)Column temp. : 40˚CCatechins in green tea121230 10 20 30 40 minColumn : Shodex Silica C18P 4DEluent : 0.1% Phosphoric acid aq./CH 3 OH=20/80Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 30˚CVitamin E34Sample :1. (-)-Epigallocatechin 1.0μg2. (-)-Catechin 1.0μg3 (-)-Epigallocatechin gallate 0.5μg4. (-)-Catechin gallate 1.0μg564Sample : 20μL1. α-Tocopherol 5μg/mL2. α-Tocotrienol 10μg/mL3. β-Tocopherol 5μg/mL4. γ-Tocopherol 5μg/mL5. γ-Tocotrienol 10μg/mL6. δ-Tocopherol 5μg/mL7. δ-Tocotrienol 10μg/mL721Silica-based Packed Columns for Reversed Phase, HILIC and Normal Phase Chromatography0 5 10 15 20 25 0 5 10 15 051015min min minColumn : Shodex Silica 5SIL 4DColumn : Shodex Silica 5PYE 4DColumn : Shodex Silica 5NPE 4DEluent : n-Hexane/Isopropanol/Acetic acidEluent : 20mM KH 2 PO 4 aq./CH 3 OH=40/60Eluent : H 2 O/CH 3 OH=30/70=1000/6/5Flow rate : 1.0mL/minFlow rate : 1.0mL/minFlow rate : 1.0mL/minDetector : UV(254nm)Detector : UV(254nm)Detector : Fluorescence(Ex. 298nm, Em. 325nm)Column temp. : 30˚CColumn temp. : 30˚CColumn temp. : 30˚C

Standard analysiscolumnsColumns for Chiral SeparationFeaturesCDα-, β-, and γ-cyclodextrin derivatives bound to packing materials as the functional groupSeparates by using differences in conformational compatibility of optical isomersSilica-based and polymer-based packing materialsVersatile columns for chiral separationNewsNo.26, 27CRX-853L-amino acids derivatives bound to the packing material as the functional groupSeparates optical isomers by using difference in capacity of metal complex formation between metalion and optical isomers after the functional group and the metal ion in eluent forms complexSuitable for amino acids, hydroxy acids, and derivatives thereof22Columns for Chiral SeparationABA-894Standard columnsProduct CodeF7146000Bovine serum albumin (BSA) bound to packing materials as the functional groupSeparates optical isomers by using interactions between the isomers captured by hydrophobicpockets of BSA and surrounding amino acid residuesSuitable for the separation of optical isomers of amino acids and carboxylic acidsProduct NameORpak CDA-453 HQFunctional Groupα-Cyclodextrin derivativeBase MaterialPolyhydroxymethacrylateParticleSize(μm)6Column Size(mm)I.D. x L4.6 × 150News No.21H 2 OSemi-microMicro ColumnsShipping Solventp.73F7146001ORpak CDB-453 HQβ-Cyclodextrin derivativePolyhydroxymethacrylate64.6 × 1501.7% TEA-acetate buffer + 0.1MNaCl(pH4.0)/CH 3CN=80/20F7146002ORpak CDC-453 HQγ-Cyclodextrin derivativePolyhydroxymethacrylate64.6 × 1501.0% Acetic acid + 0.2M NaCl aq./CH 3 CN=90/10F7146003ORpak CDBS-453β-Cyclodextrin derivativeSilica34.6 × 1501.0% Acetic acid + 0.2M NaCl aq./CH 3 CN=70/30F6709250ORpak CD-G(guard column)Polyhydroxymethacrylate64.6 × 10H 2 OF7140040ORpak CRX-853L-amino acid derivativePolyhydroxymethacrylate68.0 × 500.25mM CuSO 4 aq.F6709300ORpak CRX-G(guard column)Polyhydroxymethacrylate64.6 × 100.25mM CuSO 4 aq.F7118960AFpak ABA-894Bovine serum albuminPolyhydroxymethacrylate68.0 × 5050mM Tris-HCl buffer +150mMNaCl+ 0.02% NaN 3 (pH7.4)CH 2OHOHOOH OHCH 2 CH2 CHO 2OOOHn=6, α-CDn=7, β-CDn=8, γ-CDOHOnOHStructure of CDOHOOHOHOH*Contact Shodex or our distributors near you for customized columns.

Medicinal chemicalssampleAlprenololBrompheniramineCaprofenChlorpheniramineChlorthalidoneClidiniumbromideHomatropineIndapamideLaudanosolineNefopamNorlaudanosolineOrphenadrineOxyphencyclimineProcyclidinePromethazinePropranololTerbutalineTolperizoneTrihexyphenidylValethamate**WarfarinZopicloneEpinephrineSample : EpinephrineHO*OHk ,3.34.44.50.66.89.84.310.67.62.17.616.017.45.815.518.61.521.36.13.23.93.11.8NHα1.051.351.091.301.291.061.271.181.321.291.391.071.111.091.061.121.191.191.141.071.081.121.22OHCH3Rs0.74.21.41.43.80.82.52.32.03.42.81.21.61.21.01.31.81.61.80.91.01.21.8eluentAACCAABABABACCABABCCCCAColumn : Shodex ORpak CDBS-453Eluent : (A) (1.1% Acetic acid+0.2M NaCl) aq./CH 3CN=90/10(B) 2.2% Acetic acid+1.2% Triethylamine+ 0.2M NaCl aq.(C) (1.1% Acetic acid+0.2M NaCl) aq./CH 3 CN=70/30Flow rate : (A, C) 0.5mL/min, (B) 1.0mL/minDetector : UV(254nm)Column temp. : 20˚C* Two asymmetric centers existWarfarins( I )0 20 40 mim( II )0 20 40 mim16-hydroxy Warfarin(each 2.5μg)23S-Warfarin(125μg)7-hydroxy Warfarin(each 2.5μg)R-Warfarin(125μg)WarfarinO O*CHCH2COCH 3OHN6-hydroxy WarfarinHOHOSample :1. ChlorpheniramineCHNOOCH 3CH3CI2. Monodesmethyl-chlorpheniramineNO7-hydroxy WarfarinCHNCH3H*CHCH 2COCH 3O*CHCH 2COCH3Column : Shodex ORpak CDBS-453Eluent : (1.0% Acetic acid + 0.2M NaCl) aq./CH 3CN=80/20Flow rate : 0.4mL/minDetector : ( l ) UV(310nm)( ll ) Fluorescence(Ex. : 335nm, Em. : 415nm)Column temp. : 16.5˚CChlorpheniramine and its methabolitesStandard analysiscolumns23Columns for Chiral SeparationCI3. Didesmethyl-chlorpheniramineNCHNHH0 10 20 30 mim010 20 30 mimCIColumn : Shodex ORpak CDBS-453Eluent : (0.05% Acetic acid + 0.2M NaCl) aq./CH 3 CN=99/1Flow rate : 0.5mL/minDetector : UV(254nm)Column temp. : 10˚CColumn : Shodex ORpak CDBS-453Eluent : (1.0% Acetic acid + 0.2M NaCl) aq./CH 3 CN=90/10Flow rate : 0.5mL/minDetector : UV(254nm)Column temp. : 30˚CData provided by Dr.Sakurai,Tokyo University of Pharmacy and Life ScienceAmino acidsAmino acids, Hydroxy acids and Carboxylic acidsOptical isomersRsαEluentColumnOptical isomersRsαEluentColumn6-FluorotryptophanPhenylalanineDAmino acidsCOOHH 2 N CH CH 2*NHAlanine-β-naphthylamide*H 2 N CH CONHCH 3MTH-PhenylalanineCH 3NSO *NHCH 2F1.98 1.33 A2.43 1.60 B3.30 2.0 CORpakCDA-453 HQORpakCDB-453 HQORpakCDB-453 HQAmino acidsHydroxy acidsNH 2CH 2CH COOH*DNS-ValineH 3 CCH 3 CH 3NHCH3CSO 2 NHCH COOH*Mandelic acid*CH COOHOHSLLDR2.17 2.26 W1.58 1.43 X2.30 1.44 YORpakCRX-853AFpakABA-894ORpakCRX-853DNS-LeucineH 3 C CH 3CHH 3 CNCHH 23 CSO 2 NHCH COOH*2.79 1.97 DORpakCDC-453 HQCarboxylic acids4-(1-Oxo-2-cyclohexylmethyl)-benzoic acidO*COOH1.48 1.41 ZAFpakABA-894A : H 2 OB : [1.4% Acetic acid + 0.7% Triethylamine + 0.1M NaCl(pH3.9)]/CH 3 CN=80/20C : (1.8% Acetic acid + 0.1M NaCl) aq./CH 3 CN=80/20D : (1.1% Acetic acid + 0.2M NaCl) aq./CH 3 CN=90/10W : 0.5mM CuSO 4 aq./CH 3 CN=60/40X : 25mM Phosphate buffer(pH7.0) + 0.5% IsopropanolY : 0.25mM CuSO 4 aq.Z : 50mM Sodium phosphate buffer(pH7.0)

Standard analysiscolumnsColumns for Anion Exchange ChromatographyFeaturesQA-825,DEAE-825Suitable for the analysis of relatively high-molecular-weight compounds, such as proteins,peptides, DNA, and RNAUses eluents over a wide pH range from 2 to 12NotebookNo.7Semi-microMicro Columnsp.71PreparativeColumnsp.84DEAE3N-4TNon-porous base materialFor rapid analysisNotebookNo.6NewsNo.33Semi-microMicro Columnsp.71NEWDEAE-2BNon-porous base materialSupports UHPLC (available under hyperbaric conditions up to 30 MPa)NewsNo.4224ES-502N 7CPolyvinyl alcohol as a base material offers different separation propertiesLow hydrophobic interaction of proteins allows analysis under mild conditionsColumns for Anion Exchange ChromatographyWA-624Standard columnsSuitable for anion exchange analysis of low-molecular-weight compounds such as nucleotidesStrong anion exchange resin ( Functional Group : Quaternary Ammonium )ProductCodeF6110011 IEC QA-825 0.45 Polyhydroxymethacrylate 12 5,000 8.0 × 75 50mM Na 2 SO 4 aq.ProductCodeProduct NameProduct NameIon ExchangeCapacity(meq/g)Base MaterialWeak anion exchange resin ( Functional Group : Diethylaminoethyl )Ion ExchangeCapacity(meq/g)Base MaterialParticleSize(µm)ParticleSize(µm)PoreSize(Å)PoreSize(Å)Column Size(mm)I.D. x LColumn Size(mm)I.D. x LSemi-microMicro Columnsp.71 PreparativeColumnsShipping SolventShipping Solventp.84F6118255IEC DEAE-8250.6Polyhydroxymethacrylate85,0008.0 × 7550mM Na 2 SO 4 aq.F6112100IEC DEAE3N-4T0.4Polyhydroxymethacrylate2.5−4.6 × 35H 2 OF6112110NEW PIKESS DEAE-2B0.4Polyhydroxymethacrylate2.5−2.0 × 50H 2 OF7640002Asahipak ES-502N 7C0.55Polyvinyl alcohol92,0007.5 × 10050mM 1,3-Diaminopropane+ 50mM NaCl(pH10.0)F6356240AXpak WA-6241.2Polyhydroxymethacrylate102,0006.0 × 1500.1M Sodium phosphate buffer(pH3.0)/CH 3 CN=80/20F6700245AXpak WA-G(guard column)Polyhydroxymethacrylate10−4.6 × 100.1M Sodium phosphate buffer(pH3.0)/CH 3 CN=80/20*Contact Shodex or our distributors near you for customized columns.

Protein separation using QA-8251234Sample : 100μL1.Conalbumin 0.02%2.Transferrin 0.17%3.Ovalbumin 0.14%4.Trypsin inhibitor 0.17%Effect of eluent pH for DEAE-825pH6.0pH7.0314342210 5 10 15 min0 5 10 15 minSample : 100μL1. Conalbumin 0.06%2. Transferrin 0.27%3. Ovalbumin 0.33%4. Trypsin inhibitor 0.33%Standard analysiscolumnspH8.012 34pH9.01 432pH10.01,234015 30 min0 5 10 15 min 0 5 10 15 min 0 5 10 15 minColumn : Shodex IEC QA-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0)(B); (A) + 0.5M NaClLinear gradient; 100%(A) to 50%(A), 30minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Comparison data of porous DEAE-825 and non-porous DEAE3N-4TIEC DEAE3N-4T is a weak anion exchange column. Diethyaminoethyl group is on the surface of anon-porous gel. Rapid analysis of proteins and peptides are possible with non-porous packing materials.Its peak shape is still sharp, when a small volume of the sample is injected.1porous gel DEAE-82520 5 10 15 20 25 30 minColumn : Shodex IEC DEAE-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 60minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Injection vol. : 100μL3Column : Shodex IEC DEAE-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0), 20mM Tris-Trispropanol-HCl buffer(pH7.0)20mM Tris-HCl buffer(pH8.0), 20mM Ethanolamine-HCl buffer(pH9.0)20mM 1,3-Diaminopropane-HCl buffer(pH10.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 20minFlow rate : 1.0mL/minnon-porous gel DEAE3N-4T3: Diethylaminoethyl 1: DiethylaminoethylColumnEluent20 5 minFlow rateDetectorColumn temp. : Room temp.Injection vol. : 20μLSample :1. Conalbumin 0.4mg/mL2. Ovalbumin 1.2mg/mL3. Trypsin inhibitor 1.0mg/mL: Shodex IEC DEAE3N-4T: (A); 25mM Piperazine-HCl buffer(pH6.0)(B); A + 0.5M NaClLinear gradient; (A) to (B), 10min: 1.5mL/min: UV(280nm)Gibberellin IsomersHOHOOC2. GA14HOHOOC1. endo GA14CH3CH3COOHCOOH0 10 20 min12Sample :GibberellinColumn : Shodex Asahipak ES-502N 7CEluent : Acetic acid/H 2 O/CH 3 OH=0.1/0.4/99.5Flow rate : 1.5mL/minData was provided byDetector : UV(210nm)Prof. Yamaguch,Column temp. : 50˚CFaculty of Agliculture,University of Tokyo.25Columns for Anion Exchange ChromatographyMercaptoalbumins andnon-mercaptoalbuminsNucleotidesSulfide ion and cyanide ion123Sample :Normal control serum1. Globulin2. Uric acid3. HMA4. HNA12453678 9Sample :1. 5'CMP2. 5'UMP3. 5'TMP4. 3'CMP5. 5'ADP6. 3'UMP7. 5'GMP8. 2'AMP9. 3'AMP10. 3'GMP1Sample :1. HS - 0.1mg/L2. CN - 0.02mg/L24100 8 16 24 min 0 5 10 15 2025 min0.0 1.5 3.0 4.5 6.0 7.5 9.0 10.5 12.0 13.5 15.0 16.5 18.0minColumn : Shodex Asahipak ES-502N 7CEluent : 50mM N-methylpiperazine-HClbuffer(pH4.8) + 400mM Na 2 SO 4+ 0.3% C 2 H 5 OHFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 35˚CColumn : Shodex AXpak WA-624Eluent : 0.35M Ammonium acetate buffer(pH4.3)Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : 60˚CColumn : Shodex IEC DEAE-825Eluent : 10mM Na 2 CO 3 + 1mM Ethylenediamine+ 10% CH 3 OHFlow rate : 1.0mL/minDetector : EC(Electrode; Silver, 0mV SCE)Column temp. : 25˚C

Standard analysiscolumnsColumns for Cation Exchange ChromatographyFeaturesSP-825, CM-825Suitable for the analysis of relatively high-molecular-weight compounds, such as proteins,peptides, DNA, and RNAUses eluents over a wide pH range from 2 to 12No.7NotebookSemi-microMicro Columnsp.72 PreparativeColumnsp.84SP-420NNon-porous base materialFor rapid analysisSemi-microMicro Columnsp.72NEWSP-2BNon-porous base materialSupports UHPLC (available under hyperbaric conditions for up to 30 MPa)NotebookNo.6NewsNo.4226Columns for Cation Exchange Chromatography Column for Hydrophobic Interaction ChromatographyES-502C 7CP-421SStandard columnsStrong cation exchange resin ( Functional Group : Sulfopropyl )ProductIon ExchangeProduct Name CapacityBase MaterialCode(meq/g)F6118250 IEC SP-8250.4 PolyhydroxymethacrylateF6113000F6113110F7640001IEC SP-420NNEWPIKESS SP-2BAsahipak ES-502C 7C0.30.3PolyhydroxymethacrylatePolyhydroxymethacrylateWeek cation exchange resin ( Functional Group : Carboxymethyl )ProductIon ExchangeProduct Name CapacityBase MaterialCode(meq/g)F6110002 IEC CM-8250.4 PolyhydroxymethacrylateFor amino acidsProductCodeF6354211F6700210FeaturesPH-814Product NameCXpak P-421SCXpak P-GStandard columnPolyvinyl alcohol as a base material offers different separation propertiesLow hydrophobic interaction with proteins allowsanalysis under mild conditionsNo.7Columns for Amino acid analysis in the strong cation exchange modeSupports simultaneous analysis of different amino acids0.55Plate Number(TP/column)≥ 3,500(guard column)Polyvinyl alcoholFunctionalGroupSulfo(Na + )Sulfo(Na + )Base MaterialParticleSize(μm)82.52.5ParticleSize(μm)89Styrene divinylbenzenecopolymerStyrene divinylbenzenecopolymerPoreSize(Å)5,000−−PoreSize(Å)5,0002,000ParticleSize(μm)6Separates proteins without denaturationApplicable to samples after treatment of ammonium sulfate fractions6Column Size(mm)Shipping SolventI.D. x L8.0 × 75 50mM Na 2SO 4 aq.20mM Sodium acetate buffer4.6 × 35+ 0.5M Na 2 SO 4 (pH5.0)20mM Sodium acetate buffer2.0 × 50+ 0.5M Na 2 SO 4 (pH5.0)Column Size(mm)Shipping SolventI.D. x L8.0 × 75 50mM Na 2 SO 4 aq.0.1M Sodium phosphate buffer7.5 × 100(pH4.4)Column Size(mm)I.D. x L4.6 × 1504.6 × 10Shipping SolventColumn for Hydrophobic Interaction ChromatographyNotebookSemi-microMicro Columnsp.72 PreparativeColumnsH 2 OH 2 ONotebookp.84No.3ProductCodeProduct NameFunctionalGroupBase MaterialParticleSize(μm)PoreSize(Å)Column Size(mm)I.D. x LShipping SolventF6110003 HIC PH-814 Phenyl Polyhydroxymethacrylate 10 2,000 8.0 × 75H 2 O*Contact Shodex or our distributors near you for customized columns.

Protein separation using cation exchange columns( I ) CM-825(weak cation ion exchange)90μL injected12345( II ) SP-825(strong cation ion exchange)30μL injected1436 65( III ) SP-420N(strong cation ion exchange)non-porous type gel456Rapid analysis of proteins using UHPLC123456Sample : 5μL (13mg total protein )1.Myoglobin2. Trypsinogen3. Ribonuclease A4. α-Chymotrypsinogen A5. Cytochrome c6. LysozymeStandard analysiscolumns21230.01.0 2.0 3.0 4.0 min0 15 30 45 min 0 15 30 min 0 2 4 6 minColumn : ( I ) Shodex IEC CM-825, ( II ) Shodex IEC SP-825,( III ) Shodex IEC SP-420NEluent : (A); 20mM Sodium phosphate buffer(pH7.0) (B); (A) + 0.5M NaCl( I , II ) Linear gradient; (A) to (B), 60min ( III ) Linear gradient; (A) to (B), 10minFlow rate : ( I , II )1.0mL/min ( III )1.5mL/minDetector : UV(280nm)Column temp. : Room temp.1234HOHOCH 2·CH·NH 2COOH2. AdrenalineHO CH·CH 2·NHHO OH CH 33. NoradrenalineHO CH·CH 2·NH 2HOOH4. DopamineHOHOCH 2·CH 2·NH 2120 20 min0 10 20 30 min0 10 20 minAspThrSerGluProSample : 300μg/mL each, 10μLGlyCysAlaValMetIleLueSample : 2.5nmol each, 100μL0 1020 30 40 50 minColumn : Shodex CXpak P-421SEluent : No.1 0.12M Sodium citrate buffer(pH3.3)No.2 0.13M Sodium citrate buffer(pH3.2)No.3 0.11M Sodium citrate buffer(pH4.0)No.4 1.02M Sodium citrate buffer(pH4.9)No.5 0.2M NaOH aq./Ethanol=90/10 (Rinse solution)Low pressure gradient;No.1 (0min), No.2 (1.2min), No.3 (10.0min), No.4 (21.2min), No.5 (40min)Flow rate : 0.5mL/minDetector : VIS(570nm)post column method (ninhydrin reaction 0.35mL/min, 120˚C)Column temp. : 63˚CTyrPheLysNH 3ArgHisSample :1. Myoglobin2. Trypsinogen3. Ribonucrease A4. α-Chymotrypsinogen A5. Cytochrome c6. LysozymeCatecholamines Angiotensins Paraquat and diquat1. DOPAColumn : Shodex Asahipak ES-502C 7CEluent : 20mM Sodium malonate buffer+ 0.5M NaCl(pH6.0)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 30˚CStandard amino acidsSample : 10μL1. Angiotensin II (human)Asp-Arg-Val-Tyr-lle-His-Pro-Phe2. Angiotensin I (human)Asp-Arg-Val-Tyr-lle-His-Pro-Phe-His-Leu3. Angiotensin III (human)Arg-Val-Tyr-lle-His-Pro-PheColumn : Shodex Asahipak ES-502C 7CEluent : 50mM Sodium malonate buffer(pH6.0)/CH 3 CN=80/20Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 30˚C1323Column : Shodex PIKESS SP-2BEluent : (A); 20mM Sodium phosphatebuffer(pH 7.0)(B); (A) + 0.5 M NaClLinear gradient; 100%(A) to 50%(A),2.5minFlow rate : 1.2mL/minDetector : UV(280nm)Column temp. : 25˚CSample : 20μLParaquat+ +H 3 C N N CH 3 2Cl - Diquat+ 2Br -N + NColumn : Shodex Asahipak ES-502C 7CEluent : 50mM Sodium phosphate buffer+ 150mM NaCl(pH7.0)Flow rate : 1.0mL/minDetector : UV(288nm)Column temp. : 30˚CProtein separation using hydrophobic interaction chromatography0 20 40 60 minColumn : Shodex HIC PH-814Eluent : (A); 1.8M Ammonium sulfate + (B)(B); 0.1M Phosphate buffer(pH7.0)Linear gradient; (A) to (B), 60minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.456Sample : 370μL1. Cytochrome c 0.03%2. Myoglobin 0.08%3. Ribonuclease A 0.16%4. Ovalbumin 0.16%5. Lysozyme 0.04%6. α-Chymotrypsinogen 0.05%27Columns for Cation Exchange Chromatography Column for Hydrophobic Interaction Chromatography

Standard analysiscolumnsColumns for Affinity ChromatographyFeaturesAFpak Rigid polymer-based packing materials enable high speed analysisBinds functional group through chemically stable ligands (spacers)Minimizes detachment of functional groups, ensuring highly repeatable analysisSemi-microMicro Columnsp.73-76Standard columnsProductCodeProduct NameLigandLigand Load / gel(g)Particle Size(μm)Column Size(mm) I.D. x LShipping Solventsee below tableF7118948AFpak AAB-894Aminobenzamidine100μmol188.0 × 50F7118940AFpak AAF- 894Acriflavine10μmol188.0 × 50F7113000AFpak AAM-8945'AMP10μmol188.0 × 5028F7112000AFpak AAP-894Aprotinin5mg188.0 × 50Columns for Affinity ChromatographyF7118963F7118960F7118955F7118943F7118942F7118954F7118949F7118947F7118952F7113010F7118945F7118944F7113080AFpak AAV-894AFpak ABA-894AFpak ABT-894AFpak ACA-894AFpak ACB-894AFpak ADS-894AFpak AED-894AFpak AGA-894AFpak AGE-894AFpak AGT-894AFpak AHR-894AFpak AIA-894AFpak ALC-894AvidinBovine serum albumin (BSA)BiotinConcanavalin A (Con A)Cibacron blueDextran sulfateEthylenediaminediacetic acidN-acetylglucosamineGelatinGlutathioneHeparinIminodiacetic acidLentil lectin5mg20mg−15mg40μmol30mg30μmol−30mg50μmol5mg70μmol6-7mg18618181818181818181818188.0 × 508.0 × 508.0 × 508.0 × 508.0 × 508.0 × 508.0 × 508.0 × 508.0 × 508.0 × 508.0 × 508.0 × 508.0 × 50F7118962AFpak ALS-894Lysine−188.0 × 50F7113020AFpak ANA-894Nicotinamide Adenine Dinucleotide (NAD)10μmol188.0 × 50F7118958AFpak AOV-894Ovomucoid10mg188.0 × 50F7118946AFpak APA-894Protein A4mg188.0 × 50F7118950AFpak APB-894Aminophenylboronic acid800μmol108.0 × 50F7113030AFpak APD-894Procion red40-60μmol188.0 × 50F7113070AFpak APE-894Phosphorylethanolamine−188.0 × 50F7113050AFpak APG-894Protein G4-5mg188.0 × 50F7118957AFpak APH-894Phenylalanine50μmol188.0 × 50F7113040AFpak APR-894Protamine4-5mg188.0 × 50F7118956AFpak ARC-894Ricinus communis agglutinin I (RCA-I)20mg188.0 × 50F7118953AFpak AST-894Soybean trypsin inhibitor20mg188.0 × 50F7118959AFpak AWG-894Wheat germ agglutinin (WGA)14mg188.0 × 50F7118964AFpak ACH-494Choline oxydase, acetylcholine esterase−184.6 × 10F7111000AFpak AGO-494Glucose oxydase−184.6 × 10Shipping Solvent0.1M Sodium acetate buffer + 0.5M NaCl + 0.02% NaN 3 (pH7.0)0.1M Ethyl morpholine-acetic acid buffer + 0.02% NaN 3 (pH7.0)10mM Sodium phosphate buffer + 0.15M NaCl + 0.02% NaN 3 (pH7.4)50mM Tris-HCl buffer + 0.15M NaCl + 0.02% NaN 3 (pH7.0)10mM Sodium acetate buffer + 0.02% NaN 3 (pH6.5)50mM Tris-HCl buffer + 0.15M NaCl + 0.5mM MnCl 2 + 0.5mM CaCl 2 + 0.02% NaN 3 (pH7.0)0.1M Potassium phosphate buffer + 0.02% NaN 3(pH5.0)50mM Sodium phosphate buffer + 0.02% NaN3(pH7.4)50mM Ethyl morpholine-acetic acid buffer + 0.02% NaN3(pH6.0)10mM Tris-HCl buffer + 0.02% NaN 3 (pH8.0)10mM Tris-HCl buffer + 10mM NaCl + 0.02% NaN 3 (pH7.4)50mM Tris-HCl buffer + 0.1M NaCl + 1mM MnCl 2 + 1mM CaCl 2 + 0.2M Glucose+ 0.01% Merthiolate(pH7.2)0.1M Sodium phosphate buffer + 0.5M NaCl + 0.02% NaN 3 (pH7.0)10mM Sodium phosphate buffer + 0.1M NaCl + 0.02% NaN 3 (pH7.0)20mM Sodium phosphate buffer + 0.02% NaN 3 (pH6.0)0.1M Sodium phosphate buffer + 0.15M NaCl + 0.2M Galactose + 0.02% NaN 3(pH7.4)0.1M Tris-HCl buffer + 0.15M NaCl + 0.2M N-Acetylglucosamine + 0.02% NaN 3(pH7.4)10mM Phosphate buffer + 1.0M NaCl(pH7.4)50mM Phosphate buffer + 0.15M NaCl(pH6.0)*Contact Shodex or our distributors near you for customized columns.

ApplicationProduct NameAFpak AAB-894Serine protease, etcApplicationProduct NameAFpak ALC-894ApplicationGlycoproteins, polysaccharides, etcStandard analysiscolumnsAFpak AAF-894RNA, DNA, vitamins, etcAFpak ALS-894Plasminogen, Plasminogen activator, RNA, DNA, etcAFpak AAM-894NAD-dependent dehydrogenase,ATP-dependent kinase, etcAFpak ANA-894NAD- dependent dehydrogenase, etcAFpak AAP-894Serine protease, etcAFpak AOV-894Trypsin-like protease, etcAFpak AAV-894Biotin, Biotin derivativesAFpak APA-894IgG, immune complexes, etcAFpak ABA-894Chiral separation (amino acid), etcAFpak APB-894Nucleic acids, catecholamines, etcAFpak ABT-894Avidin, etcAFpak APD-894NAD, NADP-dependent enzymes, interferon, etcAFpak ACA-894Glycoproteins, polysaccharides, etcAFpak APE-894C-reactive proteins, etc29AFpak ACB-894AFpak ADS-894AFpak AED-894AFpak AGA-894AFpak AGE-894AFpak AGT-894AFpak AHR-894AFpak AIA-894CapacityAlbumin, NAD dependent enzymes, etcLipoproteins, blood coagulation factors, etcNucleic acids, serum proteins, etc.Lectins, carbohydrate metabolizing enzymes, etcFibronectin, etcEnzymes related to glutathione, etcLipoproteins, blood coagulation factors, etcInterferon, serum proteins, etcAFpak APG-894AFpak APH-894AFpak APR-894AFpak ARC-894AFpak AST-894AFpak AWG-894AFpak ACH-494AFpak AGO-494IgG, immune complexes, etcAlkalophilic protease, etcIgM, etcGlycoproteins, polysaccharidesTrypsin-like protease, etcGlycoproteins, polysaccharidesCholine, acetylcholine(Enzyme-substrate immobilized column)Glucose (Enzyme-substrate immobilized column)Columns for Affinity ChromatographyProduct Name Capacity Product NameCapacityAFpak AAB-894—AFpak ALC-894—AFpak AAF-894ATP Na1.8mg/columnAFpak ALS-894Plasminogen54μg/gAFpak AAM-894Lactic dehydronase1.5mg/gAFpak ANA-894Lactic dehydronase1.4mg/gAFpak AAP-894Trypsin3mg/gAFpak AOV-894Trypsin100mg/gAFpak AAV-894Biotin8μg/columnAFpak APA-894IgG Human13mg/gAFpak ABA-894—AFpak APB-894Sorbitol0.2mg/columnAFpak ABT-894Avidin9mg/gAFpak APD-894BSA72mg/gAFpak ACA-894—AFpak APE-894C-reactive protein0.91mg/gAFpak ACB-894BSA20mg/columnAFpak APG-894IgG Humnan10mg/gAFpak ADS-894LDL5mg/gAFpak APH-894Subtilisin Carlsberg9mg/gAFpak AED-894—AFpak APR-894IgM Human1.9mg/columnAFpak AGA-894Lysozyme0.6mgAFpak ARC-894—AFpak AGE-894Fibronectin150mg/gAFpak AST-894Trypsin100mg/gAFpak AGT-894—AFpak AWG-894—AFpak AHR-894Lysozyme4mg/columnAFpak ACH-494—AFpak AIA-894BSA70mg/columnAFpak AGO-494—See p.30 for applications.

Standard analysiscolumnsPrinciple of affinity chromatographySampleinjectionAdsorption of thetarget substanceCleaningElution of thetarget substanceBiotin 4-aminobenzoic acidSample :Biotion 4-aminiobenzoic acidLigand : AvidineAdsorptionWashingElutionEluent AEluent B0 10 20 30 min30Columns for Affinity Chromatographybase gelspacerSample :Trypsin(Sigma)Ligand :Aminobenzamideligand12target substanceSample :1. p-Nitrophenyl-α-D-galactoside2. p-Nitrophenyl-α-D-glucoside3. p-Nitrophenyl-α-D-mannoside3Ligand : Concanavalin AColumn : Shodex AFpak AAV-894Eluent : (A); 0.01M Sodium acetate buffer+ 0.15M NaCl(pH7.0)(B); 0.1M Acetic acid aq./Isopropanol=50/50Step gradient; (A) to (B)Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : Room temp.Trypsin p-Nitrophenol-glucosides Lipoproteins in plasmaEluent AEluent BLDLEluent A Eluent BHDLEluent A0 10 20 30 min 0 10 20 30 minEluent BVLDLEluent AEluent BSample :Lipoproteins(Sigma)Ligand : Dextran sulfate0 2040 minColumn : Shodex AFpak AAB-894Eluent : (A); 0.1M Sodium acetate buffer+ 0.5M NaCl(pH7.0)(B); 0.1M Sodium acetate buffer+ 0.5M NaCl(pH2.8)Step gradient; (A) to (B)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.0 20 40 minColumn : Shodex AFpak ACA-894Eluent : 0.02M Tris-HCl buffer+ 0.25M NaCl(pH7.4)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.0 10 20 30 40 minColumn : Shodex AFpak ADS-894Eluent : (A); 0.05M Sodium phosphate buffer(pH7.4)(B); (A) + 1.0M NaClStep gradient; (A) to (B)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Fibrinogen L-Lactic dehydrogenase ChymotrypsinSample : Fibrinogen(Sigma)Ligand : Lentil lectinSample : L-Lactic dehydrogenaseLigand : NAD(Nicotinamide-adenie dinucleotide)Sample :Chymotrypsin(Sigma)Ligand :Soybean trypsin inhibitorEluent AEluent BEluent BEluent AEluent AEluent B0 20 40 60 min0 20 40 60 minColumn : Shodex AFpak ALC-894Eluent : (A); 50mM Tris-HCl buffer+ 0.15M NaCl + 0.5mM CaCl 2+ 0.5mM MnCl 2 (pH7.4)(B); (A) + 0.1M Methyl mannosideStep gradient; (A) to (B)Flow rate : 0.5mL/minDetector : UV(280nm)Column temp. : Room temp.0 5 10 15 20 minColumn : Shodex AFpak ANA-894Eluent : (A); 10mM Potassium phosphatebuffer(pH7.0)(B); (A) + 0.5M KClStep gradient; (A) to (B)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Column : Shodex AFpak AST-894Eluent : (A); 0.1M Sodium acetate buffer+ 0.5M NaCl(pH7.7)(B); 0.1M Sodium acetate buffer+ 0.5M NaCl(pH2.8)Linear gradient; 0 to 20min, (A)20 to 40min, (A) to (B)after 40min (B)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.

Column Trouble ShootingCommon causes for abnormal chromatogramsInformationUnstable baseline values······Main cause······Solution······Frequent problemPeak tailingSelection ofcolumn and/or eluentappropriateNOYESStrong sample retentionVoid existing at top of columnChange column and/or eluentReplace columnPeak split,shouldersAdsorption of impurities incolumnChanneling in column· Wash column· Check column performance· Replace columnReplace column31PeakleadingBroad peakGhost peakProblem solvedby reducinginjection volumeNOYESLeading caused by sampleproperties(ionic and micellar properties)· Adsorption of impurities incolumn· Deterioration of columnSample overloadContaminationImpurities existing in eluentChange column and/or eluent· Wash column· Check column performance· Replace columnReduce injection volumePrepare sample againWash flow line of HPLCReplace eluentColumn Trouble ShootingNo peakSystem trouble· Eluent not flowing· Injector at wrong position (load/inject)· Detector deactivatedCheck systemSample adsorption in columnRepeat sample injection· Column equilibration insufficientPressure elevationPumpA B CColumn Detector······Main causeSTARTInjector······Solution······Frequent problemSalt precipitated isobserved in eluentYESPressure still highafter washingNOSalting out· Change eluent composition· Wash system before analysisNOYESPressure still high whenC is disconnectedNODetector pipingblocked· Clean up detector· Replace pipingYESPressure still high whenB is disconnectedYESNOColumnblockedPressure undermaximum columnpressureYESNOScreen eluent and samplethrough 0.45m filterYESNODeteriorationof columnColumn end-fittingblocked· Check column performance· Replace columnReplace columnPressure still high whenA is disconnectedNOInjector blocked· Clean up injector· Replace injectorYESUsed line filterYESNOPump piping blocked· Replace filter in pump· Clean up pump· Replace pump pipingLine filter blocked· Wash line filter· Replace line filter

Standard analysiscolumnsColumns for Ligand Exchange Chromatography* A list of elution volumes of saccharides for our columns is available.Please refer to our website (http://www.shodex.com/) or technical notebook (No.2 and 3).FeaturesSC1011, 1821,SP0810,KS-801, 802Separates saccharides by combining ligand exchange and size exclusion modesThree types of counter ions are available: Ca, Pb, and Na ionsSolvents other than water are not required for the analysis of neutral sugarsNotebook No.3 News No.6, 40, 43[KS-801, 802]PreparativeColumnsp.85KS-803~807Suitable for separation of polysaccharides in the size exclusion modeAvailable in combination with KS-802 and KS-801Solvents other than water are not required for the analysis of neutral sugarsNotebookNo.3, 8, 13[KS-803~806]PreparativeColumnsp.8532Columns for Ligand Exchange ChromatographyMN-431EP SC1011-7FStandard columnsLigand exchange and Size exclusionColumn for the analysis of mannitol in conformity with USPColumn for Ca-type ligand exchange chromatographySolvents other than water are not required for analysisColumn for Ca-type ligand exchange chromatographyColumn for the analysis of sugar alcohols and saccharides in conformity with EPPlate Number Functional groupProduct Code Product Name(TP/column) (Counter Ion)F6378102 SUGAR SC1011≥ 13,000 Sulfo(Ca 2 + )F6378103 SUGAR SC1821≥ 13,000 Sulfo(Ca 2 + )F6700090 SUGAR SC-LG (guard column) Sulfo(Ca 2 + )F6378105 SUGAR SP0810≥ 11,000 Sulfo(Pb 2 + )F6700081 SUGAR SP-G (guard column) Sulfo(Pb 2 + )F6378010 SUGAR KS-801≥ 17,000 Sulfo(Na + )F6378020 SUGAR KS-802≥ 17,000 Sulfo(Na + )F6378025 SUGAR KS-803≥ 17,000 Sulfo(Na + )F6378035 SUGAR KS-804≥ 17,000 Sulfo(Na + )F6378050 SUGAR KS-805≥ 9,000 Sulfo(Na + )F6378060 SUGAR KS-806≥ 9,000 Sulfo(Na + )F6700020 SUGAR KS-G (guard column) Sulfo(Na + )F6378070 SUGAR KS-807≥ 4,000 Sulfo(Na + )F6700021 SUGAR KS-807G (guard column) Sulfo(Na + )For the United States Pharmacopeia (USP)Product Code Product NameFunctional group(Counter Ion)F6379230 USPpak MN-431Sulfo(Ca 2 + )Exclusion Limit(Pullulan)1,00010,000−1,000−1,00010,00050,000400,0005,000,000(50,000,000)*−(200,000,000)*−Particle Size(μm)8ParticleSize(μm)Column Size(mm)I.D. x L6610710666717171030308.0 × 3008.0 × 3006.0 × 508.0 × 3006.0 × 508.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3006.0 × 508.0 × 3008.0 × 50H 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2OH 2 OH 2 OH 2 OH 2 O*( )Estimated valueBase Material : Styrene divinylbenzene copolymerColumn Size(mm)I.D. x L4.0 × 250Shipping SolventShipping SolventH 2 OSee p.69 for USP column list.Base Material : Styrene divinylbenzene copolymerFor the European Pharmacopoeia (EP)Product CodeProduct NameFunctional group(Counter Ion)Particle Size(μm)Column Size(mm)I.D. x LShipping SolventF6379230EP SC1011-7FSulfo(Ca 2 + )87.8 × 300H 2 OBase Material : Styrene divinylbenzene copolymer*Contact Shodex or our distributors near you for customized columns.

FeaturesDC-613,SC1211,SZ5532Separation is performed by combining ligand exchange and HILIC modesDC-613 can analyze sugars without removing sodium salts in the sampleSC1211 is suitable for separation of sugar alcoholsSZ5532 is recommended for the separation of disaccharides or trisaccharides[DC-613]Standard analysiscolumnsNotebookNo.2PreparativeColumnsp.85Standard columnsLigand exchange and HILICProduct CodeF7001003F6700170F7001400F6700120F7001300F6700110SubstabcesArabinoseD-ArabitolDulcitolmeso-ErythritolProduct NameRSpak DC-613RSpak DC-GSUGAR SC1211SUGAR SC-GSUGAR SZ5532SUGAR SZ-G≥ 5,500(guard column)≥ 5,500(guard column)≥ 5,500(guard column)SP0810 SC1011 KS-801 SZ553210.4215.8620.1812.708.9111.3312.7610.098.217.637.407.86Plate Number(TP/column)Elution Volume (mL)5.117.279.465.73NH2P-504E6.186.297.455.43Functional group(Counter Ion)Sulfo(Na + )Sulfo(Na + )Sulfo(Ca 2 + )Sulfo(Ca 2 + )Sulfo(Zn 2 + )Sulfo(Zn 2 + )SC1211 DC-6135.568.1611.286.276.036.067.814.95Particle Size(μm)Elution volume of saccharides analysis with various columns[Partial list only; refer to website for complete list]61061066SubstabcesD-MannoseMelibioseNystosePalatinitPore Size(Å)100−50−100−Column Size(mm)I.D. x L6.0 × 1504.6 × 106.0 × 2504.6 × 106.0 × 1504.6 × 10H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=75/25H 2 O/CH 3 CN=75/25H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=30/70Base Material : Styrene divinylbenzene copolymerSP0810 SC1011 KS-801 SZ553210.728.166.382peaks8.176.455.452peaksElution Volume (mL)7.645.984.935.905.8311.6920.052peaksNH2P-504EShipping SolventSC1211 DC-6137.84 5.0114.70 4.2331.90 *12.73 2peaks7.1516.39—2peaks33Columns for Ligand Exchange ChromatographyD(-)-Fructose11.058.857.715.376.755.906.52Palatinose7.846.455.898.0812.123.9910.56D(+)-Fucose10.488.848.094.505.434.965.04Panose7.145.785.3216.8725.60*25.89D(+)-Galactose9.747.987.586.468.104.987.91D(+)-Raffinose7.145.785.2916.3620.25*21.60Gentiobiose7.226.085.7510.5016.36*14.45Rhamnose9.778.237.373.935.524.434.23Glucose8.637.307.175.878.614.767.35D(-)-Ribose19.3513.669.044.825.458.645.55myo-Inositol12.778.867.9912.639.967.8717.36D(-)-Sorbitol21.6113.317.429.797.0911.887.68Isomaltose7.686.265.9510.5715.18*15.24Sorbose9.678.037.385.127.354.926.15Isomaltotriose7.095.755.3421.1727.55*37.45Stachyose6.825.574.97—36.22*—1-Kestose6.795.755.2613.0920.11*11.11Sucrose7.546.295.877.9111.87*9.45Kojibiose7.566.215.889.6514.82*12.70α-D-Talose21.3312.598.765.696.478.516.86Lactitol13.278.096.1316.3511.826.6715.26Trehalose7.626.275.7810.8513.25*12.42Lactose8.056.515.9910.1213.274.0713.04Trehalulose8.926.956.109.5411.684.7812.41Lactulose9.136.996.199.1610.724.6511.88Xylitol19.8713.147.947.776.1010.166.44Maltitol12.238.266.0313.0411.826.7713.12Xylobiose8.166.686.405.659.05*7.04Maltose7.856.345.948.6714.24*11.46D(+)-Xylose9.217.907.714.556.584.485.35Maltotriose7.485.895.3813.7924.96*19.92D-Xylulose10.649.028.044.065.415.074.65Mannitol15.8011.107.238.757.399.037.22(—) Not detected ( * ) Overlap with solvent peak(—) Not detected ( * ) Overlap with solvent peakColumn : SUGAR SP0810, SC1011, KS-801Eluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : 80˚CColumn : SUGAR SC1211Eluent : H 2O/CH 3CN=65/35Flow rate : 1.0mL/minDetector : RIColumn temp. : 70˚CColumn : SUGAR SZ5532Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 60˚CColumn : Asahipak NH2P-50 4EEluent : H 2O/CH 3CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 30˚CColumn : RSpak DC-613Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 70˚C

Standard analysiscolumnsComparison of saccharides analysis based on different counter ionsKS-801012 3 45 6 78SC101112 3410 20 min 0 10 20 min6785In the ligand exchange mode, the elutionpattern depends on the kind of counter ion.Therefore, select the column applicable toanalyze the combination of saccharides.Refer list of "Elution volume of variouscolumns for saccharides analysis" (p33)Sample : 5μL1. Pullulan P10 0.5%2. Maltotriose 1.0%3. Maltose 1.0%4. Glucose 1.0%5. Sorbitol 1.0%6. Fructose 1.0%7. Glycerol 1.0%8. EtOH 2.0%Ketohexoses12Sample : 0.025% each, 10μL1. Sorbose2. Fructose3. Tagatose4. Psicose34SP08106·834Columns for Ligand Exchange Chromatography010 10 20min0 10 20min243710 20 30 40 minAnomer separation of saccharidesSaccharides may affect a chromatogram through anomer separation.When using a SUGAR column to analyze saccharides, the analysis at hightemperatures would suppress the influence of anomer separation, resulting infavorable chromatograms.Glucose Mannose Xylose Galactose Arabinose29˚C70˚C0 10 20min0 10 20min0 10 20min0 10 20min50 10 20min0 10 20min0 10 20minGlucose Mannose Xylose Galactose ArabinoseColumn : Shodex SUGAR SC1011Eluent : H 2 OFlow rate : 0.7mL/minDetector : RIColumn temp. : 29˚C, 70˚C0 10 20minColumn : Shodex SUGARKS-801SC1011SP0810Eluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 80˚CSample :0.5% each, 10μLMolecular Weight (Pullulan)0 5 10 15 20 minColumn : Shodex SUGAR SP0810Eluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : 80˚CCalibration curves for KS-800 series using pullulan10 710 610 510 410 310 246 810 12KS-801KS-802KS-803KS-804KS-805KS-806Elution volume (mL)Column : Shodex SUGAR KS-800 seriesEluent : H 2 ODetector : RIColumn temp. : 80˚CHydrolyzed starchAcesulfame K and saccharidesSucralose and saccharidesDp-5Dp-1Sample : 20μLHydrolyzed starch 1%Sample : 10μL1. Acesulfame K 0.005%2. Sucrose 0.5%3. Glucose 0.5%4. Fructose 0.5%12SucraloseCl CH2OHCH2ClOOHOOOH OHOHCH 2ClDp-10123434Sample : 1% each,10μL1. Sucrose2. Glucose3. Fructose4. meso-Erythritol5. SucraloseRI5UV(254nm)1015 20 min0510 15 min5 10 1520 minColumn : Shodex SUGAR KS-802 x 2Eluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : 80˚CColumn : Shodex SUGAR SC1011Eluent : 10mM CaSO 4 aq.Flow rate : 1.0mL/minDetector : RI, UV(254nm)Column temp. : 80˚CColumn : Shodex SUGAR SP0810Eluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : 80˚C

6 78Saccharides in wood12Sample : 5μL1. Cellobiose 1.0%2. Glucose 1.5%3. Xylose 0.5%4. Galactose 0.5%5. Arabinose 0.5%6. Mannose 1.0%Maltose and isomaltose12Sample : 15μL1. Glucose 1.0%2. Maltose 1.0%3. Isomaltose 0.6%4. Maltotriose 1.0%Standard saccharides1Sample : 2.5% each, 15μL1. Xylose2. Fructose3. Glucose4. Galactose5. Sucrose6. Maltose7. Lactose8. Melibiose9. Maltotriose10. RaffinoseStandard analysiscolumns642 343 4 5359105 10 1520 min 0 24 6 8 10 12 min0 10 20 30 minColumn : Shodex SUGAR SP0810Eluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 85˚CSaccharides in the presence of sodium saltThe RSpak DC-613 enables the analysis of saccharides in sodium-salt containingsample without desalting because the column uses Na as counter ion.Hydrolyzed samples with acids such as hydrochloric acid and sulfuric acid can beanalyzed as sample solution after being neutralized with sodium hydroxide.Solvent for samplepreparation1% NaCl aq./CH 3 CN=50/501% Na 2 SO 4 aq./CH 3 CN=50/50H 2 O/CH 3 CN=50/500 51min23Sample : 0.1% each, 10μL1. Xylose2. Sucrose3. Cellobiose4. Lactose4Column : Shodex SUGAR SZ5532Eluent : H 2 O/CH 3 CN=25/75Flow rate : 0.9mL/minDetector : RIColumn temp. : 60˚C10 15Pinitol05123Columnn : Shodex RSpak DC-613Eluent : H 2 O/CH 3 CN=20/80Flow rate : 1.5mL/minDetector : RIColumn temp. : 70˚C4Sample : 0.1% each, 10μL1. Pinitol2. Fructose3. Glucose4. Sucrose5. chiro-Inositol6. myo-Inositol5 610 1520 min35Columns for Ligand Exchange ChromatographyColumn : Shodex RSpak DC-613Eluent : H 2 O/CH 3 CN=30/70Flow rate : 0.8mL/minDetector : RIColumn temp. : 50˚C* High concentration of acetonitlie isapplied to eluent of saccharideanalysis using DC-613. Take care ofsalt concentration in sample to avoidsalt deposition from sample solution.Column : Shodex RSpak DC-613Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 70˚CMoisturizing componentsMannitol analysis with USP methodSample : 0.1% each, 20μL1. 1,3-Propanediol2. Propylene glycol3. Ethylene glycol4. Glycerin5. meso-ErythritolAccording to the USP (United States Pharmacopeia) method, mannitol should beanalyzed using a column which can separate mannitol and sorbitol with a degreeof separation equal to or greater than 2.0. The USPpak MN-431 is a columnspecially designed for mannitol analysis, which satisfies this criteria.1Sample : 10μL1. Mannitol 0.5%2. Sorbitol 0.5%2241350 51015 min0 24 6 8 minColumn : Shodex SUGAR SC1211Eluent : H 2 O/CH 3 CN=60/40Flow rate : 0.6mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex USPpak MN-431Eluent : H 2 OFlow rate : 0.5mL/minDetector : RIColumn temp. : 60˚C

Standard analysiscolumnsColumns for Ion Exclusion ChromatographyFeaturesSH1011, 1821 Columns for simultaneous analysis of saccharides and organic acidsSeparates neutral sugars in size exclusion mode, and organic acids in ion exclusion modeSuitable for the analysis of uronic and aldonic acidsNotebook No.3 News No.25, 40, 43KC-811Columns for the analysis of organic acidsIon exclusion mode (plus reversed phase mode)Highly selective detection with post column methodKC-811 6E supports the analysis of cyanide ions and cyanogen chloride in accordance with theJapanese Water Supply ActNotebook No.3 News No.6, 34p.85PreparativeColumns36Columns for Ion Exclusion ChromatographyStandard columnsFor simultaneous analysis of saccharides and organic acidsProduct CodeF6378100F6378101F6700080Product CodeF6378030Product NameSUGAR SH1011SUGAR SH1821SUGAR SH-GProduct NameRSpak KC-811Plate Number(TP/column)≥ 17,000≥ 17,000(guard column)For organic acids, cyanide ions and cyanogen chloridePlate Number(TP/column)≥ 17,000FunctionalGroupSulfoSulfoSulfoFunctionalGroupSulfoExclusion Limit(Pullulan)1,00010,000−Particle Size(μm)6ParticleSize(μm)6610Column Size(mm)I.D. x L8.0 × 3008.0 × 3006.0 × 50H 2 OH 2 OH 2 OBase Material : Styrene divinylbenzene copolymerColumn Size(mm)I.D. x L8.0 × 300Shipping SolventShipping Solvent0.1% H 3 PO 4 aq.F6378033RSpak KC-811 6E≥ 13,000Sulfo66.0 × 2500.1% H 3PO 4 aq.F6700030RSpak KC-G(guard column)Sulfo106.0 × 500.1% H 3 PO 4 aq.F6700010RSpak KC-LG(guard column)Sulfo138.0 × 500.1% H 3 PO 4 aq.* As a guard column, use KC-LG for samples with relatively low purityand KC-G for samples with relatively high purity.Base Material : Styrene divinylbenzene copolymer*Contact Shodex or our distributors near you for customized columns.

Maltooligosuccharides, organic acidsand ethanol12 34567Sample :0.05% each, 20μL1. Maltotetraose2. Maltotriose3. Maltose4. Glucose5. Lactic acid6. Glycerol7. Acetic acid8. Methanol9. EthanolCello-oligosaccharides and furfurals1 2 34567Sample : 0.1% each, 10μL1. Cellopentaose2. Cellotetraose3. Cellotriose4. Cellobiose5. Glucose6. Glyceric acid7. Acetic acid8. Furfural9. 5-HMF8Standard organic acidsSample :1. Citric acid2. Tartaric acid3. Pyruvic acid4. Malic acid5. Succinic acid6. Glycolic acid7. Lactic acid8. Fumaric acid9. Acetic acid10. Levulinic acid11. Pyroglutamic acid12. Propionic acid13. Isobutyric acid14. n-Butyric acid2 4135679810111213 14Standard analysiscolumns9980 5 10 15 20 25 0 5 10 15 20 25 30 35 40 4510min min min20 30Column : Shodex SUGAR SH1821Eluent : 0.5mM H 2 SO 4 aq.Flow rate : 0.6mL/minDetector : RIColumn temp. : 75˚CHighly hydrophobic organic acids12 3Eluent = 2mM HClO 4 aq.42052567830 35 40 minEluent = 2mM HClO4 aq./CH3CN=90/10Sample :1. Succinic2. Lactic3. Formic4. Acetic5. Propionic6. Isobutyric7. n-Butyric8. Isovaleric9. n-ValericTo shorten analysis time9Column : Shodex SUGAR SH1821Eluent : 2mM H 2 SO 4 aq.Flow rate : 0.6mL/minDetector : RIColumn temp. : 60˚COrganic acids and vitamin C in fruit juice21345 101520 min56Sample :1. Cl - ,etc.2. Oxalic acid3. Maleic acid4. Citric acid5. Malic acid6. Vitamin C7. Succinic acid8. Formic acid9. Acetic acid789Organic acids in black vinegarUV(210nm)RIColumn : Shodex RSpak KC-811 x 2Eluent : 6mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 50˚C1122Sample : Black vinegar, 20μL1. Gluconic acid2. Lactic acid3. Acetic acid4. Pyroglutamic acid334437Columns for Ion Exclusion Chromatography15202530 min510 15 20 25 30minColumn : Shodex RSpak KC-LG + KC-811 x 2Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 47˚CColumn : Shodex RSpak KC-LG + KC-811 x 2Eluent : 1mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 40˚CColumn : Shodex RSpak KC-811x 2Eluent : 3mM HClO 4 aq.Flow rate : 1.0mL/minDetector : UV(210nm), RIColumn temp. : 50˚COrganic acids in Japanese sakeCyan and cyanogen chloride withpost column methodSample : 100μL1. Phosphoric acid etc.2. Citric acid3. Pyruvic acid4. Malic acid5. Succinic acid6. Lactic acid7. Fumaric acid8. Acetic acid9. Pyroglutamic acid56Fine rice wine56Fermented liquorPure rice wine546CN -Sample : 10μg/L each, 100μLCNCl14114238792378 9238792 4 6 8 10 min 2 4 6 8 10 min0 5 10 15 20 25min0 5 10 15 20minColumn : Shodex RSpak KC-LG + KC-811 x 2Eluent : 4.8mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 63˚C25 0 5 10 15 20 25minColumnEluentReagent AReagent BFlow rate: Shodex RSpak KC-811 6E: 1.0mM H 2 SO 4 aq.: Chloramine T solution: 4-Pyridinecarboxylic acid-Pyrazolonesolution: (Eluent) 1.0mL/min(Reagent) 0.5mL/min each: VIS(638nm)DetectorColumn temp. : 40˚CReaction temp. : (Reagent A) 40˚C(Reagent B) 80˚C

Standard analysiscolumnsColumns for Ion Chromatography (Anion analysis)FeaturesNI-424, I-524AColumns for anion analysis with non-suppressor methodNI-424 supports the simultaneous analysis of fluoride and phosphate ionsNewsNo.15SI-90, 50Columns for anion analysis with suppressor methodSuitable for the quantitative analysis of fluoride ionSI-50 separates target substances from organic acidsNot affected by the system peak derived from carbonateNewsNo.5SI-52, 91Columns for the analysis of oxyhalidesSI-52 supports simultaneous analysis of oxyhalides and general inorganic ionsSI-91 is used with the post column methodNewsNo.3438VH-anionEnables anion analysis in wine without being affected by organic acidsSupports rapid analysisNewsNo.37Columns for Ion Chromatography (Anion analysis)Standard columnsFor anions ( non-suppressor method )ProductCodeF6995243F6709616F6995240F6700400ProductCodeF6995244F6709620F6995245F6709625Product NameIC NI-424IC NI-GIC I-524AIC IA-GFor anions ( suppressor method )Product NameIC SI-90 4EIC SI-90GIC SI-50 4EIC SI-50GFor oxyhalidesProductProduct NameCodeF6995260 IC SI-52 4EF6709626 IC SI-92GF6995280 IC SI-91 4CF6709620 IC SI-90GPlate Number(TP/column)≥ 5,000Functional GroupQuaternary ammonium(guard column) Quaternary ammonium≥ 2,000 Quaternary ammonium(guard column) Quaternary ammoniumPlate Number(TP/column)Functional Group≥ 5,000 Quaternary ammonium(guard column) Quaternary ammonium≥ 10,000 Quaternary ammonium(guard column) Quaternary ammoniumPlate Number(TP/column)Functional Group≥ 14,000 Quaternary ammonium(guard column) Quaternary ammonium≥ 2,500 Quaternary ammonium(guard column) Quaternary ammoniumFor anions in wineProductPlate NumberProduct NameCode(TP/column)Functional GroupF6995263 WINE VH-anion 4D ≥ 7,000 Quaternary ammoniumF6709623 WINE VH-anionG 4A (guard column) Quaternary ammoniumParticle Size(μm)551212Particle Size(μm)9955Particle Size(μm)5999Particle Size(μm)59Column Size(mm) I.D. x LShipping Solvent8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris4.6 × 100+ 2mM Phenylboronic acid + 0.005mM CyDTA aq.8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris4.6 × 10+ 2mM Phenylboronic acid + 0.005mM CyDTA aq.4.6 × 1002.5mM Phthalic acid aq.4.6 × 102.5mM Phthalic acid aq.Base Material : PolyhydroxymethacrylateColumn Size(mm) I.D. x L4.0 × 250 1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.4.6 × 10 1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.4.0 × 250 3.2mM Na 2 CO 3 + 1.0mM NaHCO 3 aq.4.6 × 10 3.2mM Na 2 CO 3 + 1.0mM NaHCO 3 aq.Base Material : Polyvinyl alcohol Housing material : PEEKColumn Size(mm) I.D. x L4.0 × 2503.6mM Na 2 CO 3 aq.4.6 × 103.6mM Na 2 CO 3 aq.4.0 × 100 1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.4.6 × 10 1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.Base Material : Polyvinyl alcohol Housing material : PEEKColumn Size(mm) I.D. x L4.6 × 1504.6 × 10Shipping SolventShipping SolventShipping Solvent3.2mM Na 2 CO 3 + 1.0mM NaHCO 3 aq.1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.Base Material : Polyvinyl alcohol Housing material : PEEKLine Filters for ICProductCodeProduct NameApplicationContentsF8500630IC FL-1For general purposes(not for the substitution of Y-521L)one holder and one filterF8500640IC FL-1 filterReplacement filter for IC FL-15 filtersF8500650IC FL-2non-metal type(not for the substitutionof Y-521L)one holder and one filterF8500660IC FL-2 filterReplacement filter for IC FL-24 filters*Contact Shodex or our distributors near you for customized columns.

Anion analysis with non-suppressor method (NI-424 and I-524A)NI-4241234 5 67Sample : 50μL1. H2PO - 4 20mg/L2. F - 1.5mg/L3. CI - 2.5mg/L4. NO2 - 6mg/L5. Br - 10mg/L6. NO3 - 10mg/L7. SO4 2- 10mg/LI-524A1,234567NI-424 is a high-performance columnwith a theoretical plate number whichis approximately twice as large asthan that of I-524A.(1) Enables the separation of H 2 PO 4-and F - ions which were difficult toseparate with I-524A.(2) The shape of each peak is sharper,and the separation balance isfavorable. Improvements havebeen made especially in theseparation of Cl - and NO 2- ions.Standard analysiscolumns0 2 4 6 8 10 12 14 16 min02 4 6 8 10 12 14 min0Column : Shodex IC NI-424Eluent : 8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris+ 2mM Phenylboronic acid + 5μM *CyDTA(pH4.2)Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C*CyDTA : trans-1,2-Diaminocyclohexane-N,N,N',N'-tetra acetic acidAnion analysis with suppressor method using SI-90 4E1235456710 min8Sample : 20μL1. F - 2mg/L2. Cl - 3mg/L3. NO2 - 5mg/L4. Br - 10mg/L5. NO3 - 10mg/L6. HCO3 - 300mg/L7. PO4 3- 15mg/L8. SO4 2- 15mg/LColumn : Shodex IC I-524AEluent : 2.5mM Phthalic acid + 2.3mM Tris(pH4.2)Flow rate : 1.2mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CAnion analysis with suppressor method using SI-50 4ESI-50 4E is a high-performance version of SI-90 4E.Acetic acid, formic acid, and methacrylic acid areeluted later than F - ion and faster than CI - ion. Thecarbonate system peak appears between NO 2- andBr - peaks.12 3456780 5 10 15 20 25 30 min910Sample : 20μL1. F - 2mg/L2. Acetic acid 10mg/L3. Formic acid 2mg/L4. Methacrylic acid 10mg/L5. Cl - 3mg/L6. NO2 - 5mg/L7. Br - 10mg/L8. NO3 - 10mg/L9. PO4 3- 15mg/L10. SO4 2- 15mg/L11. Oxalic acid 15mg/L1139Columns for Ion Chromatography (Anion analysis)Column : Shodex IC SI-90 4EEluent : 1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.Flow rate : 1.2mL/minDetector : Suppressed conductivityColumn temp. : 25˚CColumn : Shodex IC SI-50 4EEluent : 3.2mM Na 2 CO 3 + 1.0mM NaHCO 3 aq.Flow rate : 0.7mL/minDetector : Suppressed conductivityColumn temp. : 25˚COxyhalides analysis with suppressor method using SI-52 4E145 691011Sample : 50μL1. F - 2mg/L2. ClO2 - 1mg/L3. BrO3 - 1mg/L4. Cl - 10mg/L5. NO2 - 5mg/L6. Br - 10mg/L7. ClO3 - 1mg/L8. Dichloroacetate 1mg/L9. NO3 - 30mg/L10. PO4 3- 15mg/L11. SO4 2- 40mg/LSI-52 4E is a high-resolution column exhibiting a number oftheoretical plates of 14,000 or higher. It supports simultaneousanalysis of Oxyhalides and inorganic anions.The recommended temperature is 45˚C.Calibration curves of Oxyhalides with the SI-52 4Epeak area (μs/cm*sec)2.521.510.50ChloriteR 2 =0.994910 20 30 40 50 60 70 80 90 100ClO 2- concentration(μg/mL)peak area (μs/cm*sec)21.81.61.41.210.80.60.40.200ChlorateR 2 =0.999510 20 30 40 50 60 70 80 90 100ClO 3- concentration(μg/mL)2 37 805 10 15 20 25 30 minColumn : Shodex IC SI-52 4EEluent : 3.6mM Na 2 CO 3 aq.Flow rate : 0.8mL/minDetector : Suppressed conductivityColumn temp. : 45˚Cpeak area (μs/cm*sec)Bromate1.41.2R 2 =0.998510.80.60.40.200 10 20 30 40 50 60 70 80 90 100BrO - 3 concentration(μg/mL)

Standard analysiscolumnsColumns for Ion Chromatography (Cation analysis)FeaturesYS-50Higher performance type of YK-421Applicable to both analyses with suppressor and non-suppressor methodsPeak shape is sharper, especially for divalent cation analysisSupports the analysis of alkylamines and transition metalsNotebookNo.4NewsNo.2840YK-421Y-521T-521, R-621Columns for cation analysis with non-suppressor methodSimultaneous analysis of monovalent and divalent cationsSuitable for separation of alkylaminesColumns for cation analysis with non-suppressor methodFor the separation of monovalent or divalent cationsT-521 and R-621 are applicable for the analysis of transition metals and rare earth metal ions, respectivelyHigh sensitivity analysis is developed by the post column color reaction methodColumns for Ion Chromatography (Cation analysis)Standard columnsFor cationsProductCodeF7122000F6700530F7120012F6709608F6995210F6700230ProductNameIC YS-50IC YS-GIC YK-421IC YK-GIC Y-521IC Y-GFor transition metal ionsProductCodeProductNamePlate Number(TP/column)≥ 5,500(guard column)≥ 2,800(guard column)≥ 3,000(guard column)Plate Number(TP/column)FunctionalGroupCarboxylCarboxylCarboxylCarboxylSulfoSulfoFunctionalGroupBase MaterialPolyvinyl alcoholPolyvinyl alcoholSilicaSilicaStyrene divinylbenzene copolymerStyrene divinylbenzene copolymerBase MaterialParticleSize(μm)55551212ParticleSize(μm)Column Size(mm)I.D. x L4.6 × 1254.6 × 104.6 × 1254.6 × 104.6 × 1504.6 × 10Column Size(mm)I.D. x LShipping SolventH 2 OH 2 O5mM Tartaric acid + 1mM Dipicolinicacid + 1.5g/L Boric acid aq.5mM Tartaric acid + 1mM Dipicolinicacid + 1.5g/L Boric acid aq.4mM HNO 3 aq.4mM HNO 3 aq.Shipping SolventF6995250IC T-521≥ 3,000SulfoStyrene divinylbenzene copolymer124.6 × 1503mM HNO 3 aq.F6700412IC T-G(guard column)SulfoStyrene divinylbenzene copolymer124.6 × 103mM HNO 3 aq.Housing material : PEEKFor rare earth metal ionsProductCodeProductNamePlate Number(TP/column)FunctionalGroupBase MaterialParticleSize(μm)Column Size(mm)I.D. x LShipping SolventF6998000IC R-621≥ 1,000SulfoStyrene divinylbenzene copolymer56.0 × 50H 2 OF6709090IC R-G(guard column)SulfoStyrene divinylbenzene copolymer54.6 × 10H 2 OLine Filters for ICProductCodeProduct NameApplicationColumn Size(mm)I.D. x LContentsF8500630IC FL-1For general purposes (not for the substitution of Y-521L)−one holder and one filterF8500640IC FL-1 filterReplacement filter for IC FL-1−5 filtersF8500650IC FL-2non-metal type (not for the substitutionof Y-521L)−one holder and one filterF8500660IC FL-2 filterReplacement filter for IC FL-2−4 filtersF6700240IC Y-521LFor the analysis of monovalent cations (used with Y-521)8.0 × 50—*Contact Shodex or our distributors near you for customized columns.

Standard cations (YS-50 and YK-421)YS-50 10μL inj.5μS/cm3241565μS/cmYK-42112315μL inj.46Sample :1. Li + 2mg/L2. Na + 10mg/L3. NH + 4 10mg/L4. K + 20mg/L5. Mg 2+ 10mg/L6. Ca 2+ 20mg/L5YS-50 is a high-performance columnwith a theoretical plate number whichis approximately twice as large asthat of YK-421. In particular, the peakshape and quantitative performancefor divalent ions have been improved.The quantitative performance forNH 4+ in a system containing Na + athigh concentration has beenenhanced as wellResolutionfactor ofNa + and NH 4+YS-502.3YK-4211.8Standard analysiscolumns05 10 15 20 min0 5 10 15 minTPMg 2+Ca 2+YS-50YK-4217,000 3,2006,900 3,300Column : Shodex IC YS-50Eluent : 4mM Methanesulfonic acid aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CEffect of adding crown ether to eluentA crown ether molecule forms complex with cations.Sample : 10μLThe elution of cations (in particular, K + ) can be controlled by the 1. Li + 2mg/Ldifference in the ability of complex formation2. Na + 10mg/L3. NH4 + 10mg/L4. K + 20mg/L1 23 5. Mg 2+ 10mg/L236. Ca 2+ 20mg/L14 56518-Crown 6-ether conc.6mM3mM1mM0mM4440 5 10 15 20 minColumn : Shodex IC YS-50Eluent : 4mM Methanesulfonic acid + 18-Crown 6-ether aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C555666Column : Shodex IC YK-421Eluent : 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CSimultaneous analysis for cations and ethylenediamine46Sample : 10μL1. Li + 2mg/L2. Na + 10mg/L3. NH4 + 10mg/L4. K + 20mg/L5. Mg 2+ 10mg/L6. Ca 2+ 20mg/L7. Ethylenediamine 50mg/L0 5 10 15 20 25 minColumn : Shodex IC YS-50Eluent : 4mM Nitric acid + 1.5mM 18-Crown 6-ether aq./CH 3CN=90/10Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C741Columns for Ion Chromatography (Cation analysis)Alkylamines Alkaline earth metal ions Transition metal ions in red wine1231 2 3 4 5 6 7 8 9 10 min5 62Sample : 50μL1. NH4 + 5mg/L2. Methylamine 5mg/L3. Dimethylamine 5mg/L4. Trimethylamine 10mg/L5. Ethylamine 10mg/L6. Propylamine 10mg/L7. Butylamine 10mg/L1 2 3 4 5 6 7 8 9 10 minColumn : Shodex IC YK-421Eluent : 4mM Phosphoric acid aq. + 10% CH 3CNFlow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 25˚C74min0246231Cu 2+Zn 2+Ni 2+Co 2+Mg 2+Mn 2+ Fe 2+Cd 2+Pb 2+Ca 2+Sr 2+0 2 4 6 8 10 minSample : 100μL1. Mg 2+ 20mg/L2. Ca 2+ 50mg/L3. Sr 2+ 100mg/L4. Ba 2+ 200mg/LColumn : Shodex IC Y-521Eluent : 4mM Tartaric acid + 2mM Ethylenediamine aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C124 534Sample : Red wine(diluted 2-fold followed byaddition of 40mL HCl), 70μL1. Fe 3+2. Zn 2+3. Co 2+4. Fe 2+5. Mn 2+0 4 8 12 16 20 24 28 minColumn : Shodex IC T-521Eluent : 6mM Oxalic acid + 3mM Citric acid(pH4.1 adjusted by KOH)+ 0.1mM NaN 3 + 0.1mM NaHSO 3 aq.Reagent : 0.2mM *PAR + 3M NH 4 OH+ 1M Acetic acid aq.[Mixing coil(0.5mm I.D. x 1m)]Flow rate : 1.0mL/minDetector : VIS(530nm)Column temp. : 40˚C*PAR : 4-(2-Pyridylazo)resorcinol

Standard analysiscolumnsColumn Selection for Size ExclusionChromatography (SEC)Application Solvent ColumnPageAnalysis of biological macromolecules(proteins, peptides, nucleic acids, etc.)buffer etc.buffer etc.KW-800 seriesKW400 seriesHigh performance(solvent-saving)4444Aqueous SEC(GFC)Analysis of biological macromolecules(high MW range)Water-soluble polymers(polyacrylamide, polyethylenimine, etc.)buffer etc.buffer etc.H 2 O, bufferaqueoussolution etc.SB-800 HQ seriesSB400 seriesSB-800 HQ seriesSB400 seriesHigh performance(solvent-saving)High performance(solvent-saving)4646464642Oligosaccharide, polysaccharidesH 2 O,aqueoussolutionKS-800 seriesGS-HQ series3248Column Selection for Size Exclusion Chromatography (SEC)Organic SEC(GPC)Aqueous/OrganicSECAnalysis of general polymersPolar polymers(polyimides, polyvinylpyrrolidones etc.)Analysis at high temperature(polyethylene, polypropylene etc.)Engineering resin analysis at roomtemperature[polyamide (nylon), polyethyleneterephthalate (PET) etc.]THFTHFTHFTHFchloroformDMFDMFDMFODCB etc.ODCB etc.ODCB etc.HFIPHFIPKF-800 seriesLF seriesKF-600 seriesKF-400HQ seriesK-800 seriesKD-800 seriesSB-800 HQ seriesSB400 seriesHT-800 seriesUT-800 seriesAT-806MSHFIP-800 seriesHFIP-600 seriesGF-HQ seriesHigh linearity ofcalibration curveRapid analysis(solvent-saving)High performance(solvent-saving)Rapid analysis(solvent-saving)High performanceHFIP LF-404 58(solvent-saving)5258565652544646585858606050Guideline of AvailabilityPolarity of SolventHighLowH 2O Methanol DMSO Acetonitrile DMF THF Chloroform TolueneKW-800 series, KW400 seriesSB-800 HQ series, SB400 seriesGS-HQ seriesGF-HQ seriesKD seriesKF, K, LF series* See page 62 for the solvent replaceability of organic solvent SEC (GPC) packed columns.

Precautions for polar polymer analysisSize exclusion chromatography analysis of polar-polymer can be influenced by unexpected interactions in the column.These interactions will result in changing elution patterns and may cause the invalid molecular weight calculations.It is important to reduce them in order to obtain the accurate molecular weight distribution.InformationInterfering interactions likely to be observedInteractions between the analyte and the packing materialsHydrophobic interactionThe analyte is adsorbed into the packing material.This delays the analyte elution, and thus results inunderestimation of its molecular weight (Figure B, D).Ionic interaction(1) Ion ExclusionThe analyte is repelled from the packing material.This accelerates the analyte elution, and thus resultsin overestimation of its molecular weight (Figure A, C)(2) Ion ExchangeThe analyte is adsorbed onto the packing material.This delays the analyte elution, and thus results inunderestimation of its molecular weight (Figure B, D).Interaction within and between the analyteIonic repulsion effects observed within the multivalentmacromolecules causes structure expansionThis accelerates the analyte elution, and thus results inoverestimation of its molecular weight (Figure A).Association between the moleculesAssociated molecule detected as a larger molecule(Figure A).Interactions between the analyte and the solventThe multivalent ion of the solvent works as a bridge tobind ionic molecules (analyte).Exclusion limitMWlog MWElution will beaccelerated.Exclusionvolume(C)(A)Elution will beretarded.(B)Permeationvolume(D)43Precautions for polar polymer analysisMethods to reduce interactionsOrganic solvent SEC (GPC) columnsAqueous SEC (GFC)Ionic InteractionAdd salt(Example) Add LiBr to DMFAdd CF 3 COONa to HFIPIonic InteractionAdd saltHydrophobic interactionLower the polarity of the eluent(Example) Change the eluent from DMF to THFHydrophillic interactionHydrophobic interactionIncrease dissociation of the analyteCationic polymer Lower the pHAnionic polymer Higher the pHLower the eluent polarity(Example) Add acetonitrile or methanolIncrease the polarity of the eluent(Example) Change the eluent from THF to DMF

Standard analysiscolumnsAqueous SEC (GFC) columns : Silica-basedFeaturesKW-800Silica-based packed columns for aqueous SEC (GFC) analysisSuitable for the analysis of proteins and enzymesNotebookNo.7NewsNo.16PreparativeColumnsp.85KW400Reducing particle size of the packing material enhances column performanceThree- or four-fold higher sensitivity than KW-800 seriesKW405-4F is applicable to samples with a molecular weight above 1 millionNotebookNo.5, 7NewsNo.31Semi-microMicro Columnsp.7744Aqueous SEC (GFC) columns : Silica-basedStandard columnsProduct CodeF6989000F6989103F6989104F6700131F6989201Product NamePROTEIN KW-802.5PROTEIN KW-803PROTEIN KW-804PROTEIN KW-GKW402.5-4FPlate Number(TP/column)≥ 21,000≥ 21,000≥ 16,000(guard column)High performance semi-micro columnsProduct CodeProduct NamePlate Number(TP/column)≥ 35,000(Pullulan)60,000170,000500,000−(Pullulan)Exclusion LimitExclusion Limit60,000(Protein)150,000700,000(1,000,000)*−(Protein)150,000ParticleSize(μm)5577ParticleSize(μm)3MaximumPore Size(Å)4001,0001,500−4008.0 × 3008.0 × 3008.0 × 3006.0 × 50Base Material : SilicaUsable pH range : pH3.0-7.5The maximum usable concentration is100% for methanol and acetonitrile.* Estimated value within parenthesisMaximumPore Size(Å)Column Size(mm)I.D. x LColumn Size(mm)I.D. x L4.6 × 300ShippingSolventH 2OH 2 OH 2 OH 2 OShippingSolventH 2 OF6989202KW403-4F≥ 35,000150,000600,00038004.6 × 300H 2 OF6989203KW404-4F≥ 25,000500,000(1,000,000)*51,5004.6 × 300H 2 OF6989204KW405-4F≥ 25,0001,300,000(20,000,000)*52,0004.6 × 300H 2 OF6700132KW400G-4A(guard column)−−5−4.6 × 10H 2OMolecular weight range with protein (eluent : phosphate buffer)Base Material : SilicaUsable pH range : pH3.0-7.5The maximum usable concentration is100% for methanol and acetonitrile.* Estimated value within parenthesisMolecular weight (Protein)10 2 10 3 10 4 10 5 10 6 10 7 10 8KW-802.5, 402.5KW-803, 403KW-804, 404KW405CalibrationStandardsSee page 63 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for KW400 series and KW-800 seriesMolecular weight (Protein)10 610 510 410 3KW402.5-4FKW404-4FKW405-4FKW403-4FMolecular weight (Protein)10 610 510 4KW-802.510 3KW-803KW-804Recovery rate of proteinsRecovery (%)ProteinKW402.5-4F KW403-4Fγ- GlobulinBovine serum albuminOvalbuminMyoglobinCytochrome cLysozyme988989909287969697899298Standard analysiscolumns10 7 Elution volume (mL)10 2 1.5 2.0 2.5 3.0 3.5 4.0 4.510 7 4510 25 6 7 8 9 10 11 12 13Elution volume (mL)α-Chymotrypsinogen A9594Comparison of KW402.5-4F and KW-802.5Sample : 10μL1. Blue dextran 2000 0.2mg/mL2. γ-Globulin 0.8mg/mL3. Ovalbumin 0.8mg/mL4. Myogrobin 0.56mg/mL5. Uridine 0.04mg/mLN=17,000(A) KW402.5-4F 1 2 34(B) KW-802.5Column : Shodex KW400-4F series, Shodex PROTEIN KW-800 seriesEluent : 50mM Sodium phosphate buffer + 0.3M NaCl(pH7.0)Flow rate : (KW400) 0.33mL/min(KW-800) 1.0mL/minDetector : (KW400) UV(280nm) (small cell volume)(KW-800) UV(280nm) (conventional type)Column temp. : 25˚CKW400 series are high-performance semi-micro columnswith theoretical plate number which is approximately 1.5times larger and a detection sensitivity (peak height) thatis 3 to 4 times higher than what KW-800 columns have.N=8,2001 2 3 455Milk whey in yogurtSample : Whey, 5μLColumn : Shodex KW402.5-4F, KW403-4FEluent : 50mM Sodium phosphate buffer+ 0.3M NaCl(pH7.0)Flow rate : 0.33mL/minDetector : UV(280nm) (small cell volume)Column temp. : 25˚CLectinsSample : 5μL1. Lectin from Soybean 0.6mg/mL2. Lectin from Arachis hypogaea 1.1mg/mL3. Lectin from Canavalia ensiformis (Con A) 0.9mg/mL4. Lectin from Lens culinaris (LCA) 0.7mg/mL5. Lectin from Triticum vulgaris (WGA) 0.8mg/mL213 4 5Aqueous SEC (GFC) columns : Silica-based05 10 15 min 20 30 4050 min 0 5 10 minColumn : (A) Shodex KW402.5-4F(B) Shodex PROTEIN KW-802.5Eluent : 50mM Sodium phosphate buffer+ 0.3M NaCl(pH7.0)Flow rate : (A) 0.33mL/min, (B) 1.0mL/minDetector : UV(280nm) (small cell volume)Column temp. : 25˚CColumn : Shodex KW402.5-4F + KW403-4FEluent : 50mM Sodium phosphate buffer+ 0.3M NaCl(pH7.0)Flow rate : 0.20mL/minDetector : UV(280nm) (small cell volume)Column temp. : 30˚CColumn : Shodex KW402.5-4FEluent : 50mM Sodium phosphate buffer+ 0.3M NaCl(pH7.0)Flow rate : 0.33mL/minDetector : UV(220nm) (small cell volume)Column temp. : 30˚CComparison of standard protein separation usingvarious GFC columnsSample :A : Thyroglobulin (bovine)B : γ-Globulin (bovine)C : Ovalbumin (chicken)D : Nyoglobin (horse)E : CyanocobalaminSB-803 HQGF-510 HQKW-803BAACBACDB0 5 10 15 20 25 30minDCDEESEC columns for an aqueous system, i.e., SB-803 HQ,GF-510 HQ and KW-803, were compared with eachother in terms of the separation of standard proteins.The result demonstrates that the KW-803, a silica-basedcolumn, is the most suitable for such an application.E35Molecular weight (Protein)10 610 5KW-803GF-510 HQSB-803 HQ10 411 13 15 17 19 21 minColumn : Shodex OHpak SB-803 HQ, Asahipak GF-510 HQ,PROTEIN KW-803Eluent : 0.2M Phosphate buffer(pH6.9)Flow rate : 0.5mL/minDetector : UV(280nm)Column temp. : 30˚CProteins in human blood serum3610 15 20 min 10 15 20 30 minColumn : Shodex PROTEIN KW-803 x 2Eluent : 50mM Phosphate buffer + 0.3M NaCl(pH7.0)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.12345678Sample : Human serum 0.1% each1. Fibrinogen 50μL2. α 2 -Macroglobulin 50μL3. IgG 50μL4. Transferrin 50μL5. Plasminogen 50μL6. Albumin 100μL7. Antitrypsin 100μL8. Hemoglobin 100μL

Standard analysiscolumnsAqueous SEC (GFC) columns : Polymer-basedFeaturesSB-800 HQPolymer-based packed columns for aqueous SEC (GFC) analysisSupports a wide range of molecular weightsThe eluent can be substituted with DMF (in columns other than SB-802 HQ andSB-807 HQ), enabling the analysis of polar polymersSB-804HQ and SB-805HQ are capable of determining the mean molecular weightof the gelatin compliant with PAGI method (ver. 10, Japan)NewsSemi-microMicro ColumnsPreparativeColumnsNo.29, 39p.78, 79p.85SB-807 HQColumns for the analysis of water-soluble ultra-high molecular polymerPacking materials with a large particle size are packed in order to prevent the breakage of molecularchainsSB400Three- or four-fold higher sensitivity than the SB-800 HQ seriesSB401-4E can analyze small molecules with MW below 1,000NewsNo.38, 3946Aqueous SEC (GFC) columns : Polymer-basedStandard columnsProduct CodeF6429100F6429101F6429102F6429103F6429104F6429105F6429106F6709430F6429108F6709431OHpak SB-802 HQOHpak SB-802.5 HQOHpak SB-803 HQOHpak SB-804 HQOHpak SB-805 HQOHpak SB-806 HQOHpak SB-806M HQOHpak SB-GOHpak SB-807 HQOHpak SB-807G≥ 12,000≥ 16,000≥ 16,000≥ 16,000≥ 12,000≥ 12,000≥ 12,000(guard column)SB-806M HQ is a mixed-gel column capable of analysis ofsamples over a wide range of molecular weight distribution.F6429111F6429112F6429113F6429114F6709432Product NameSB401-4ESB402.5-4ESB403-4ESB404-4ESB400G-4APlate Number(TP/column)For water-soluble ultra-high molecular polymer≥ 1,500(guard column)High performance semi-micro columnsProduct CodeProduct NamePlate Number(TP/column)≥ 5,000≥ 20,000≥ 20,000≥ 20,000(guard column)Exclusion Limit(Pullulan)4,00010,000100,0001,000,000(4,000,000)*8661013MaximumPore Size(Å)1002008002,0007,000Column Size(mm)I.D. x L8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3000.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.(20,000,000)*(20,000,000)*−13131015,00015,000−8.0 × 3008.0 × 3006.0 × 500.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.Base Material : Polyhydroxymethacrylate Usable pH range : pH3~10*( )Estimated value(500,000,000)*−Particle Size(μm)353530,000−8.0 × 3008.0 × 50Base Material : Polyhydroxymethacrylate*( )Estimated valueShipping SolventH 2 OH 2 OUsable pH range : pH3~10Exclusion Limit Particle SizeMaximum Column SizePore Size (mm)(Pullulan) (μm)(Å) I.D. x LShipping Solvent1,00010,000100,0001,000,000−106677402008002,000−4.6 × 2504.6 × 2504.6 × 2504.6 × 2504.6 × 10H 2 OH 2 OH 2 OH 2 OH 2 OBase Material : Polyhydroxymethacrylate Usable pH range : pH3~10Usable concentration of organic solventsProduct NameSB401-4ESB-802 HQSB-802.5 HQ, SB-803 HQSB402.5-4E, SB403-4ESB-804 HQ~SB-806M HQ,SB404-4ESB-G, SB400G-4ASB-807 HQ, SB-807GThe maximum usable concentration (%)Methanol Acetonitrile DMF0010000100757530757575301001001000Note :The maximum solvent tolerance to the SB-800 HQ series preparativecolumns(SB-2000 series) to methanol, acetonitrile, and DMF is 50%.(SB-2002 is not tolerant to solvents, similar to SB-802 HQ.)CalibrationStandardsSee page 63 forCalibration StandardsMolecular weight range with pullulan (eluent : ultrapure water)Molecular weight (Pulullan)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9SB401SB-802 HQSB-802.5 HQ, 402.5SB-803 HQ, 403SB-804 HQ, 404SB-805 HQSB-806 HQSB-807 HQSB-806M HQMolecular weight range with PEG/PEO (eluent : DMF)Molecular weight (PEG/PEO)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9SB-802.5 HQ, 402.5SB-803 HQ, 403SB-804 HQ, 404SB-805 HQSB-806 HQSB-806M HQ*Contact Shodex or our distributors near you for customized columns.

Calibration curves for SB400 series and SB-800 HQ series using pullulan (H 2 O)Molecular weight (Pullulan)10 610 510 410 3SB403-4ESB402.5-4ESB401-4ESB404-4EMolecular weight (Pullulan)10 610 510 410 310 2SB-802 HQSB-806 HQSB-805 HQSB-803 HQSB-802.5 HQSB-804 HQSB-806M HQCalibration curves for SB-800 HQ series usingPEG and PEO (DMF)Molecular weight (PEG/PEO)10 610 510 410 3SB-803 HQSB-804 HQSB-802.5 HQSB-805 HQSB-806M HQSB-806 HQStandard analysiscolumns10 7 Elution volume (mL)10 7 Elution volume (mL)10 7 Elution volume (mL)4 5 6 7 8 9 10 11 1210 2 0.5 1.0 1.5 2.0 2.5 3.0 3.510 2 4 5 6 7 8 9 10 11 12Column : Shodex SB400 series, Shodex OHpak SB-800 HQ seriesEluent : H 2 OFlow rate : 0.3mL/min, 1.0mL/minDetector : (SB400) RI(small cell volume)(SB-800 HQ) RI(conventional type)Column temp. : Room temp.SEC analysis of humic substanceCalibration curve of Sodium polystyrene sulfonateHUMIC ACID(Dando soil)HUMIC ACID(Inogashira soil)FULVIC ACID(Dando soil)FULVIC ACID(Inogashira soil)7 9 11 13 15 17 minVoVtThe left graph shows the results of SECanalysis of reference materials (from theJapanese Humic Substances Society) ofhumic acid and fulvic acid prepared frombrown forest soil (Dando) and kurobokusoil (Inogashira).Sample : Humic substances, 30μLHumic acid 0.02mg/mLFulvic acid 0.02mg/mLColumn : Shodex OHpak SB-G + SB-805 HQEluent : 10mM NaH 2 PO 4 + 10mM Na 2 HPO 4 (pH7.0) + 25% CH 3 CNFlow rate : 0.8mL/minDetector : UV(260nm)Data provided by Nobuhide Fujitake,Column temp. : 40˚CProfessor of the Graduate School of Agricultural Science, Kobe University0Column : Shodex OHpak SB-800 HQ seriesEluent : 20mM LiBr in DMFFlow rate : 0.8mL/minDetector : RIColumn temp. : 40˚CCarboxymethylcelluloseMw=101 x 10 4Mn=10.5 x 10 4Mw/Mn=9.6Sample : Carboxymethylcellulose(Medium viscosity) 0.1%, 200μL2 4 6 8 10 12 14 16 18 20 22 24 26 28 30minColumn : Shodex OHpak SB-806M HQ x 2Eluent : 0.1M NaCl aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚C47Aqueous SEC (GFC) columns : Polymer-basedEffect of sodium nitrate concentration ineluent on the analysis of polyallylaminePolycarbonatePolyacrylamideIn the analysis of cationic polymers such as polyallylamine,the concentration of sodium nitrate in the eluent being0.02M would allow the column to adsorb the mainchain of polyallylamine, resulting in the completeprevention of the elution of polyallylamine. However, ifthe concentration is 0.1M or higher, adsorption of thesample is suppressed and a favorable chromatogramcan be obtained.Sample : PolycarbonateCH 3O C O CnCH 3O1.0x10 8SB-806 HQSample : Polyacrylamide, 100μLSB-807 HQSample : Polyallylamine 0.2%, 100μL0.1 M 0.02 M0.2 MMolar Mass(g/mol)1.0x10 71.0x10 6 6.0 8.0 10.0 12.0 14.0Mw: 5,500,0000.05 M1.0x10 54.0Volume(mL)8.012.0 16.0 20.0 24.0Volume (mL)8 12 16 20 minColumn : Shodex OHpak SB-806M HQ x 2Eluent : 0.5M Acetic acid + NaNO 3 aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex OHpakSB-805 HQ + SB-802.5 HQEluent : 5mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex OHpak SB-807 HQ, SB-806 HQEluent : 0.2M NaCl aq.Flow rate : 0.5mL/minDetector : RIMALS(Multi angle laser light scattering)Column temp. : 30˚C

Standard analysiscolumnsMultimode columnsFeaturesAsahipak GS-HQSEC mode is the main modeMultimode columns for reversed phase, HILIC, and ion exchange modes are availabledepending on selected eluent conditionsSuitable for the separation of peptides or nucleic acids with similar molecular weightsSuitable for desalting samples or substituting buffer in protein analysisNotebookNo.3NewsNo.19, 20Semi-microMicro Columnsp.80PreparativeColumnsp.86Asahipak GS-320 7EAsahipak GS-620 7G-PFor the analysis of nucleic acids as “Umami” and its metabolite with isocratic elutionColumns to determine mean molecular weight distribution of gelatin compliant with PAGImethod (ver. 10, Japan)48MSpak GS-320Semi-micro columns for Asahipak GS-320 HQEffective for the analysis of chemical substances in a biological sample by columnswitching intended to remove proteinsApplicable to analysis using LC/MS or ICP-MSNewsNo.2Multimode columnsStandard columnsProduct CodeProduct NameF7600005 Asahipak GS-220 HQF7600006 Asahipak GS-320 HQPlate Number(TP/column)≥ 19,000≥ 19,000Exclusion Limit(Pullulan)3,000*40,000ParticleSize(μm)66MaximumPore Size(Å)150400Column Size(mm)I.D. x L7.5 × 3007.5 × 300Shipping SolventH 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30F7600007Asahipak GS-520 HQ≥ 18,000300,00072,0007.5 × 300H 2 O/CH 3 OH=70/30F7600008Asahipak GS-620 HQ≥ 18,000(2,000,000)**77,0007.5 × 300H 2 O/CH 3 OH=70/30F6710019Asahipak GS-2G 7B(guard column)−9−7.5 × 50H 2O/CH 3OH=70/30Molecular weight range with Pullulan (eluent : ultrapure water)Molecular weight (Pulullan)10 10 2 10 3 10 4 10 5 10 6 10 7GS-220 HQGS-320 HQGS-520 HQGS-520 HQBase Material : Polyvinyl alcoholUsable pH range : pH2~12(GS-220 HQ : pH 2~9)Usable concentration of methanol is up to 100%(GS-220 HQ up to 30%)Usable concentration of acetonitrile is up to 50%* PEG equivalent**( )Estimated valueColumns for the analysis of nucleic acids as “Umami”Product CodeProduct NameScope of applicationColumn Size(mm) I.D. x LShipping SolventF7610005Asahipak GS-320 7E“Umami” (nucleic acids)7.5 × 250H 2 O/CH 3 OH=70/30F6710019Asahipak GS-2G 7B(guard column)7.5 × 50H 2 O/CH 3 OH=70/30Columns to determine the molecular weight distribution of gelatinProduct CodeProduct NameScope of applicationColumn Size(mm) I.D. x LShipping SolventF7600023Asahipak GS-620 7G-PGelatin for photo film7.5 × 500H 2 O/CH 3 OH=70/30F6710019Asahipak GS-2G 7B(guard column)7.5 × 50H 2 O/CH 3 OH=70/30Semi-micro columnsProduct CodeProduct NamePlate Number(TP/column)Particle Size(μm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping SolventF7600130MSpak GS-320 4B≥ 2,00064004.6 × 50H 2 OF7600140MSpak GS-320 4D≥ 7,00064004.6 × 150H 2 OF7600150MSpak GS-320 2D≥ 4,00064002.0 ×1 50H 2 OCalibrationStandardsSee page 63 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for GS-HQ series usingpullulan and PEGMolecular weight10 510 410 310 2 4 5 6 7 8 9 10 11 1210 6 Elution volume (mL)GS-320 HQGS-620 HQGS-520 HQGS-220 HQPullulanPEGPeptidesGS-HQ columns can be used not only for SEC (GFC)in an aqueous system, but also for multimodalanalysis where hydrophobic interaction and ionicinteraction are used together as separation criteria,under certain conditions of the eluent. This resultsin unprecedented separation analysis.GS-HQ columns are excellent in the performancefor separating hydrophilic peptides, in particular,acidic or basic peptides, from each other.Sample :No.123456Val-Glu-Glu-Ala-GluGlu-Ala-GluVal-Glu-Ser-GluArg-AspGly-His-LysArg-Pro-Lys-ProMW576347390289340497Σf1.780.390.830.680.293.24pl3.023.123.126.759.9511.44Molecular weight10 3GFC132410 2 21 36556Standard analysiscolumnsΣf : Hydrophobic parameterpl : Isoelectric point4Column : Shodex Asahipak GS-HQ seriesEluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CLow molecular weight, water-soluble dietary fiberMonosaccharides, disaccharides,and sugar alcohols are eluted withretention times larger than thatindicated by the arrow. Thisfacilitates the separation of thosesubstances from indigestiblefractions, making thedetermination of small,water-soluble dietary fiber moreefficient.Column : Shodex Asahipak GS-320 HQEluent : 50mM Ammonium acetate buffer(pH7.0)Flow rate : 0.5mL/minDetector : UV(220nm)Column temp. : 30˚CAnalysis of the purine base in beersguanine10 15 20 25 minPurine bodies in food are analyzed after being neutralized to a purine base by hydrolyzing thehomogenized and freeze-dried food with a 70% perchloric acid. This section presents an example ofanalysis of purine base in ordinary beer and purine base in beer treated with guanase (an enzyme thatdegrades guanine to xanthine). The following data indicates that guanine was decreased and xanthinewas increased by guanase.Normal beerGuanase treated beer49Multimode columnsxanthinexanthine1015 20 25 30 35 40 45 50min010 20 30 40 50 min 0 10 20 30 40 50 minColumn : Shodex Asahipak GS-220 HQ x 2Eluent : H 2 OFlow rate : 0.5mL/minDetector : RIColumn temp. : 60˚CColumn : Shodex Asahipak GS-320 HQEluent : 150mM Sodium phosphate buffer(pH2.5)Flow rate : 0.6mL/minDetector : UV(260nm)Column temp. : 35˚CData provided by Prof. Kiyoko Kaneko,Faculty of Pharmaceutical Sciences,Teikyo University“Umami”A multimode column GS-320 7E supports an easy andsimultaneous analysis of major nucleic acids that arepresent in taste components, i.e., IMP, GMP, andAMP, and derivatives thereof with isocratic elution.Sample :1. IMP2. GMP3. AMP4. Inosine5. Hypoxanthine6. Guanosine7. Guanine8. Adenosine9. Adenine321456789Gelatin analysis with PAGI methodSample : 100μLGelatin(Koepff 16922), 0.2%High sensitivity analysis of metal-bindingamino acidsSample : 100nL1. γ-Glutamylmethylselenocysteine2. MethylselenocysteineCOOH2H12 NCOOHHNCOOHOSeCH 3g-glutamyl-Se-methylselenocysteine(gGluMeSeCys)SeCH 3H 2 NSe-methylselenocysteine(MeSeCys)5 10 15 20 25 30 35 40 0 5min10 15 20 25 30Retention time (min)0 102030 minColumn : Shodex Asahipak GS-320 7EEluent : 10mM Sodium phosphate buffer(pH5.0)Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : 30˚CColumn : Shodex Asahipak GS-620 7G-P x 2Eluent : 0.1M KH 2PO 4 aq./0.1M Na 2HPO 4 aq.=50/50Flow rate : 1.0mL/minDetector : UV(230nm)Column temp. : 50˚CColumn : Shodex GS320A-M5DEluent : 50mM CH 3COONH 4 buffer(pH6.5)Flow rate : 2.0μL/minDetector : ICP-MS(Se at m/z=82)Data provided by Yasumitsu Ogra,Professor of Showa Pharmaceutical University

Standard analysiscolumnsAqueous/Organic SEC columnsFeaturesAsahipak GF-HQ Polymer-based SEC column exhibits solvent durabilitySupports both aqueous and organic solventsNewsNo.2Semi-microMicro Columnsp.81PreparativeColumnsp.86MSpak GF-310Semi-micro columns for Asahipak GF-310 HQEffective for the analysis of chemical substances in biological samples by column switchingintending to remove proteinsUseful for LC/MS analysisSuitable for the analysis of surfactantsNews No.250Aqueous/Organic SEC columnsStandard columnsProduct CodeF7600001F7600002F7600003F7600004F6710018Asahipak GF-310 HQAsahipak GF-510 HQAsahipak GF-710 HQAsahipak GF-7M HQAsahipak GF-1G 7B≥ 19,000≥ 19,000≥ 11,000≥ 13,000(guard column)GF-7M HQ is a mixed-gel column capable of analysis of samplesover a wide range of molecular weight distribution.Usable solventsWater(sodium concentration)MethanolEthanolAcetonitrileTHFProduct Name0~0.5M0~100%0~100%0~100%0~100%Plate Number(TP/column)Exclusion Limit(Pullulan)40,000300,000(10,000,000)*(10,000,000)*−ParticleSize(μm)5599910 10 2 10 3 10 4 10 5 10 6 10 7 10 8GF-310 HQMaximumPore Size(Å)4002,00010,00010,000−GF-510 HQGF-7M HQColumn Size(mm)I.D. x L7.5 × 3007.5 × 3007.5 × 3007.5 × 3007.5 × 50Molecular weight range with pullulan (eluent : ultrapure water)Molecular weight (Pulullan)Base Material : Polyvinyl alcoholUsable pH range : pH2~9*( )Estimated valueGF-710 HQShipping SolventH 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30DMFAcetoneChloroform0~100%0~100%0~100%Molecular weight range with PEG/PEO (eluent : DMF)Molecular weight (PEG/PEO)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8EthylacetateDMSO0~100%0~50%GF-310 HQGF-510 HQGF-710 HQDMSO51~100%GF-7M HQNote : The solvents used for preparative columns GF-710 HQ (GS-710 20F, 20G) are water and methanol.Selection of GSM-700 20F or 20G is recommended when other solvents are used for scale-up testing with GF-710 HQ.Semi-micro columnsProduct CodeProduct NamePlate Number(TP/column)Particle Size(μm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping SolventF7600100MSpak GF-310 4B≥ 3,00054004.6 × 50H 2 OF7600110MSpak GF-310 4D≥ 10,00054004.6 × 150H 2 OF7600024MSpak GF-310 4E≥ 16,00054004.6 × 250H 2 OF7600120MSpak GF-310 2D≥ 5,50054002.0 × 150H 2OCalibrationStandardsSee page 63 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for GF-HQ series usingpullulan and PEGMolecular weight10 6 Elution volume (mL)10 510 410 3GF-510 HQGF-310 HQGF-710 HQGF-7M HQPullulanPEGCalibration curves for GF-HQ series usingpolystyreneMolecular weight (Polystyrene)10 6 Elution volume (mL)10 510 410 3GF-7M HQGF-310 HQGF-710 HQGF-510 HQPolyacrylonitrileSample : Polyacrylonitrile 0.1%(w/v), 100μLStandard analysiscolumns10 2 4 5 6 7 8 9 10 1110 2 4 5 6 7 8 9 10 110 5 10 15 20 25 30 35 40 minColumn : Shodex Asahipak GF-HQ seriesEluent : H 2OFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CComparison of standard polystyrene separation using various solventSample : 1mg/mL each, 50μLPolystyrene1. MW : 1,090,0002. MW : 190,0003. MW : 37,9004. MW : 9,1005. MW : 2,9806. MW : 500(A) THF (B) Chloroform (C) DMF12345 6 12345 6 1Column : Shodex Asahipak GF-HQ seriesEluent : ChloroformFlow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚C23 4 56Sodium polystylene sulfonateMW:104,0000 10 20 30 minMW:38,0000 10 20minColumn : Shodex Asahipak GF-710 HQ x 2Eluent : 20mM LiBr in DMFFlow rate : 0.6mL/minDetector : RIColumn temp. : 40˚CPolymers having both hydrophobic andhydrophilic moieties may exhibithydrophobic interactions with packingmaterials. When using such polymers,SEC analysis can be performed byeliminating the hydrophobic interactionthrough the addition of organic solventsto the eluent.Sample : 1mg/mL each, 50μLSodium polystylene sulfonateCH2CHSO3Nan51Aqueous/Organic SEC columns0 20 min 0 20 min 0 20 minColumn : Shodex Asahipak GF-310 HQ + GF-510 HQEluent : (A); THF, (B); Chloroform, (C); DMFFlow rate : 0.5mL/minDetector : (A),(B) UV(254nm), (C) UV(270nm)Column temp. : 30˚C0MW:8,00010 20 30 minColumn : Shodex AsahipakGF-510 HQEluent : 50mM LiCl aq./CH 3 CN=60/40Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚CTriglyceridesNonylphenol ethoxylate12Sample : 0.25% each, 20μL1. Tristearin2. Trilaurin3. Tributylin4. TriacetinGF-310 column is suitable for qualitative analysis andquantitative analysis of surfactants to be performed byadjusting the acetonitrile concentration in the eluent.(A) Eluent : H 2 O/CH 3 CN=70/30Sample :100mg/L Nonylphenol ethoxylate, 20μL(B) Eluent : CH 3 CN3451015 min0 20 40 60 80 min 5 10 15 20 minColumn : Shodex Asahipak GF-310 HQEluent : THFFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CColumn : Shodex Asahipak GF-310 HQEluent : (A); H 2 O/CH 3 CN=70/30, (B); CH 3 CNFlow rate : 0.6mL/minDetector : UV(220nm)Column temp. : 40˚C

Standard analysiscolumnsOrganic SEC (GPC) columns(General Analysis) : THF, chloroformFeaturesKF-800, K-800Standard organic solvent SEC (GPC) columnSupports a wide range of applications from low to high-molecular-weightcompoundsPreparativeColumnsp.86, 87Standard columns52Organic SEC (GPC) columns (General Analysis) : THF, chloroformKF-800 series : Shipping solvent tetrahydrofuran(THF)Product CodeF6028010F6028020F6028025F6028030F6027030F6028040F6027040F6028050F6027050F6028060F6028090F6027060F6028070F6027070F6700300F6709350F6028110F6028120F6028125F6028130F6028194F6028140F6028195F6028150F6028196F6028160F6028190F6028197F6028170F6028198F6700401F6709450Product NameGPC KF-801GPC KF-802GPC KF-802.5GPC KF-803GPC KF-803LGPC KF-804GPC KF-804LGPC KF-805GPC KF-805LGPC KF-806GPC KF-806MGPC KF-806LGPC KF-807GPC KF-807LGPC KF-GGPC KF-800DK-800 series : Shipping solvent chloroformProduct CodeProduct NameGPC K-801GPC K-802GPC K-802.5GPC K-803GPC K-803LGPC K-804GPC K-804LGPC K-805GPC K-805LGPC K-806GPC K-806MGPC K-806LGPC K-807GPC K-807LGPC K-GGPC K-800DPlate Number(TP/column)≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 11,000≥ 11,000≥ 11,000≥ 13,000≥ 11,000≥ 6,000≥ 6,000(guard column)(solvent-peak separation column)Plate Number(TP/column)≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 11,000≥ 11,000≥ 11,000≥ 13,000≥ 11,000≥ 6,000≥ 6,000(guard column)(solvent-peak separation column)Exclusion Limit(PS)1,5005,00020,00070,00070,000400,000400,0004,000,0004,000,000(20,000,000)*(20,000,000)*(20,000,000)*(200,000,000)*(200,000,000)*−−Exclusion Limit(PS)1,5005,00020,00070,00070,000400,000400,0004,000,0004,000,000(20,000,000)*(20,000,000)*(20,000,000)*(200,000,000)*(200,000,000)*−−Particle Size(μm)666667710101010101818810Particle Size(μm)666667710101010101818810* See page 54 for details of the solvent-peak separation columns. The columns with ‘L’or ‘M’ at the end of the column name aremixed-gel columns capable of a wide molecular weight distribution range of samples.MaximumPore SizeColumn Size(Å) (mm) I.D. x L50 8.0 × 300150 8.0 × 300300 8.0 × 300500 8.0 × 300500 8.0 × 3001,500 8.0 × 3001,500 8.0 × 3005,000 8.0 × 3005,000 8.0 × 30010,000 8.0 × 30010,000 8.0 × 30010,000 8.0 × 30020,000 8.0 × 30020,000 8.0 × 300−4.6 × 10−8.0 × 100*( )Estimated valueMaximumPore Size(Å)501503005005001,5001,5005,0005,00010,00010,00010,00020,00020,000−−Column Size(mm) I.D. x L8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3004.6 × 108.0 × 100*( )Estimated valueMolecular weight range with polystyrene (eluent KF-800 series : THF, K-800 series : chloroform)Molecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9KF-, K-801KF-, K-802KF-, K-802.5KF-, K-803KF-, K-804KF-, K-805KF-, K-806KF-, K-807Molecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9KF-, K-803LKF-, K-804LKF-, K-805LKF-, K-806MKF-, K-806LKF-, K-807L*Contact Shodex or our distributors near you for customized columns.

Calibration curves for KF-800 seriesusing PS standardMolecular weight (Polystyrene)10 610 510 410 3KF-802KF-801KF-806KF-804KF-803KF-802.5KF-807KF-805KF-806MCalibration curves for KF-800L (linear type)series using PS standardMolecular weight (Polystyrene)10 810 610 510 410 3KF-807LKF-806LKF-805LKF-804LKF-803LPolycarbonate resinSample : Polycarbonate resin 0.1%, 100μLCH3O C O CCH3 O nStandard analysiscolumns10 210 21010 8 25 min10 7 Elution volume (mL)5 6 7 8 9 10 11 12 131010 7 Elution volume (mL)5 6 7 8 9 10 11 121315 20 30minColumn : Shodex GPC KF-800 seriesEluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.Silicon oilSample : Silicone oil 0.1%, 200μLSilicone oil101520Column : Shodex GPC K-806M x 2Eluent : TolueneFlow rate : 1.0mL/minDetector : RIColumn temp. : 45˚CPhenoxy resinColumn : Shodex GPC KF-800L seriesEluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.Styrene isoprene ABA block copolymerSample : Styrene isoprene ABA block copolymer15 20 25 minColumn : Shodex GPC KF-806M x 2Eluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : 30˚CColumn : Shodex GPC KF-806L x 2Eluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CBionolle TM (Polybutylene succinate/adipate)Sample : 100μLPolybutylene succinate/adipate 0.2%15 20 25 30 minColumn : Shodex GPC K-806M x 2 + K-801Eluent : ChloroformFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CRecycling fractionation of lauryl stearyl thiodipropionate53Organic SEC (GPC) columns (General Analysis) : THF, chloroformSample : Phenoxy resin 0.1%, 100μLSample : 5%, 500μL1. Distearyl thiodipropionate2. Lauryl stearyl thiodipropionate3. Dilauryl thiodipropionateCH3O C OCH2 CHCH2CH3 OHnCYCLE1CYCLE2 CYCLE6 CYCLE1021310 15 20 25 min010 20Column : Shodex GPC KF-806L x 2Eluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex GPC K-LG + K-2001Eluent : ChloroformFlow rate : 3.0mL/minDetector : RIColumn temp. : 50˚C

Standard analysiscolumnsOrganic SEC (GPC) columns(General Analysis) : DMFFeaturesKD-800Standard organic solvent SEC (GPC) columnSupports a wide range of applications from low to high-molecular-weight compoundsStandard columnsKD-800 series : Shipping solvent dimethylformamide(DMF)Product CodeProduct NamePlate Number(TP/column)Exclusion Limit(PEG/PEO)Particle Size(μm)MaximumPore Size(Å)Column Size(mm) I.D. x LF6028210GPC KD-801≥ 17,0002,5006508.0 × 300F6028220GPC KD-802≥ 17,0005,00061508.0 × 300F6028225GPC KD-802.5≥ 17,00020,00063008.0 × 30054Organic SEC (GPC) columns (General Analysis) : DMF Solvent-peak separation columns for organic SEC (GPC)F6028230F6028240F6028250F6028260F6028290F6028270F6700411Molecular weight (PEG/PEO)10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9FeaturesKF-800D,K-800DF6709350F6709450GPC KD-803GPC KD-804GPC KD-805GPC KD-806GPC KD-806MGPC KD-807GPC KD-GKD-801KD-802KD-802.5KD-803KD-804GPC KF-800DGPC K-800DKD-805KD-806MKD-806KD-807Shifting the elution of monomers, polymer additives and solvent peak in low-molecular-weight regionReduces interferences to calculate the molecular weight distribution of polymers or oligomersUse this type of column in combination with a linear columnSolvent-peak separation columnsProduct CodeProduct Name≥ 17,000≥ 17,000≥ 11,000≥ 11,000≥ 13,000≥ 6,000(guard column)KF-805L, 806L, 806M, 807LK-805L, 806L, 806M, 807L70,000400,0004,000,000(20,000,000)*(20,000,000)*(200,000,000)*−* The columns with ‘M’ at the end of the column name are mixed-gel columns capable of awide molecular weight distribution range of samples.Molecular weight range with PEG/PEO (eluent : DMF)Solvent-peak separation columns fororganic SEC (GPC)CalibrationStandardsSee page 63 forCalibration StandardsColumn CombinationParticle Size(μm)1010671010101888.0 × 1008.0 × 1005001,5005,00010,00010,00020,000−Column Size(mm) I.D. x L8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3004.6 × 10*( )Estimated valueShipping SolventTHFchloroform*Contact Shodex or our distributors near you for customized columns.

Calibration curves for KD-800 seriesusing PEG/PEO standardMolecular weight (PEG/PEO)10 710 610 510 410 3KD-805KD-804KD-803KD-802.5KD-807KD-806KD-802KD-806MPolyvinylpyrrolidoneSample : Polyvinylpyrrolidone(PVP) 0.2% each(A) PVP(K-30), 400μL(B) PVP(K-60), 500μL(C) PVP(K-90), 500μL(A)(B)Melamine formaldehyde resinSample : 30μLMelamine-formaldehyde resin 1%Standard analysiscolumns10 2KD-801(C)1010 8 Elution volume (mL)6 7 8 9 10 11 120 10 20 30min10152025 minColumn : Shodex GPC KD-800 seriesEluent : DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CCelluloses10 610 510 410 310 7 6 78 9 10 11Column : Shodex GPC KD-806MEluent : 1% LiCl in DMIFlow rate : 0.5mL/minDetector : MALS, RIColumn temp. : 60˚C* DMI 1,3-dimethyl-2-imidazolidinonePotato starchSample : 100μLPotato starch in DMSO 0.1%* solved at 80˚C0 5 10 15 minColumn : Shodex GPC KD-806MEluent : 10mM LiBr in DMSO/DMF=75/25Flow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CColumn : Shodex GPC KD-806M x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚C0Sample : Cellulose ca. 0.05% each100μLEffects of solvent-peak separation columnSample : Polyvinylchloride(1)KF-806L(2)KF-806L + KF-800DCellulose is known to be difficult todissolve. A cellulose solution isprepared by repeating solventreplacement. It is reported that thelong time required for dissolution(1 to 60 days), which depends onsolvent type, the crystallinity andmolecular weight of the sample.Data provided by Mr. Masahiro Yanagisawa,Isogai Laboratory,Graduate School of Agricultural and Life Sciences,University of Tokyo.5 10 15 min0 5 10 minDOP(Dioctylphthalate)Molecular weight (Polystyrene)Column : Shodex GPC KD-802 x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CPhenoxy resin1520minColumn : Shodex GPC KD-806M x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚C10 510 410 310 2 5 6 7 8 9 10 11 12 13 14 1510 6 Retention volume (mL)KF-806LKF-806L+KF-800DSample : 250μLPhenoxy resin 0.2%Column : (1) Shodex GPC KF-806L(2) Shodex GPC KF-806L + KF-800DEluent : THFFlow rate : 1.0mL/minDetector : RI2555Organic SEC (GPC) columns (General Analysis) : DMF Solvent-peak separation columns for organic SEC (GPC)

Standard analysiscolumnsOrganic SEC (GPC) columns :Rapid Analysis, High Performance AnalysisFeaturesKF-600Approximately half of the analysis time required with standard columnsThe amount of solvent used is reduced to about a thirdImproved applicability of solvent replacementKF-400HQAbout 1.5-times better separation performance than standard columns, with higher resolutionAbout 4-times better sensitivity than that of standard columns, supporting high sensitivity analysisThe amount of solvent used is reduced to about a thirdNews No.3, 10Improved applicability of solvent replacementRapid analysis downsized columnsKF-600 seriesUse of the KF-600 series with semi-micro type devices is recommended.56Product CodeProduct NamePlate Number(TP/column)Exclusion Limit(PS)Particle Size(μm)Maximum Pore Size(Å)Column Size(mm) I.D. x LOrganic SEC (GPC) columns : Rapid Analysis, High Performance AnalysisF6028091F6028092F6028093F6028094F6028095F6028096F6028097F6028098F6028099F6700300F6028111F6028112F6028114F6028116F6028118F6028119F6028122F6700300GPC KF-601GPC KF-602GPC KF-602.5GPC KF-603GPC KF-604GPC KF-605GPC KF-606GPC KF-606MGPC KF-607GPC KF-GGPC KF-401HQGPC KF-402HQGPC KF-402.5HQGPC KF-403HQGPC KF-404HQGPC KF-405LHQGPC KF-406LHQGPC KF-G≥ 17,000≥ 17,000≥ 17,000≥ 17,000≥ 16,000≥ 7,000≥ 7,000≥ 8,000≥ 5,000≥ 25,000≥ 25,000≥ 25,000≥ 25,000≥ 25,000≥ 10,000≥ 10,0001,5005,00020,00070,000400,0004,000,000(20,000,000)*(20,000,000)*(200,000,000)*−* The columns with ‘M’ at the end of the column names are mixed-gel columns capable of awide molecular weight distribution range of samples.High performance semi-micro columnsKF-400HQ seriesProduct CodeProduct Name(guard column)Plate Number(TP/column)(guard column)1,5005,00020,00070,000400,0004,000,000(20,000,000)*−* The columns with ‘L’ at the end of the column names are mixed-gel columns capable of awide molecular weight distribution range of samples.Molecular weight range with polystyrene (eluent : THF)333331010101883333310108501503005001,5005,00010,00010,00020,000−501503005001,5005,00010,000−6.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1504.6 × 10Shipping solvent : tetrahydrofuran(THF)*( )Estimated valueUse of the KF-400HQ series with semi-micro type devices is recommended.Exclusion Limit(PS)Particle Size(μm)Maximum Pore Size(Å)Column Size(mm) I.D. x L4.6 × 2504.6 × 2504.6 × 2504.6 × 2504.6 × 2504.6 × 2504.6 × 2504.6 × 10Shipping solvent : tetrahydrofuran(THF)*( )Estimated valueMolecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9KF-601KF-602KF-602.5KF-603KF-604KF-605KF-606Molecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9KF-401HQKF-402HQKF-402.5HQKF-403HQKF-404HQKF-405LHQKF-406LHQKF-607KF-606MCalibrationStandardsSee page 63 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for KF-600 seriesusing PS standardMolecular weight (Polystyrene)10 710 610 510 410 3KF-604KF-603KF-602.5KF-602KF-601KF-606KF-605KF-606MCalibration curves for KF-400HQ seriesusing PS standardMolecular weight (Polystyrene)10 7 Elution volume (mL)10 610 510 410 310 2KF-404HQKF-403HQKF-402.5HQKF-401HQKF-402HQStyrene-Acrylonitrile copolymerSample : Styrene-acrylonitrile (30:70) copolymerStandard analysiscolumns10 21.0 1.5 2.0 2.5 3.0 3.5 4.0Elution volume (mL)101.0 1.5 2.0 2.5 3.0 3.5 4.06 8 10 12 14minColumn : Shodex GPC KF-600 seriesEluent : THFFlow rate : 0.5mL/minDetector : UV(254nm)Column temp. : Room temp.Comparison of standard, rapid analysis and high performace semi-micro columnsStandard typeKF-802.5 x 250μL injectionHigh performance typeKF-402.5HQ x 210μL injectionRapid analysis typeKF-602.5 x 210μL injection056.0mLColumn : Shodex GPC KF-400HQ seriesEluent : THFFlow rate : 0.3mL/minDetector : UV(254nm)Column temp. : Room temp.5.7mL21mL10 15 20 25 minLC/MS analysis of additives (Irganox1010) in polymerSample : EPON1001 0.2%The analysis of the additives that are present in polymer usually includes a pretreatment step. However, our SECcolumns can separate the additives from the polymer and introduce only the low-molecular-weight fractioncontaining the additives into MS, thus enabling easy and highly sensitive analysis without any pretreatment step.UV(210nm)Sample : Cup of instant noodles (styrene foam) 1000mg/L, 5μLm/z=1176(Negative)IRGANOX 1010Column : Shodex GPC KF-606M x 2Eluent : 10mM LiBr in DMFFlow rate : 0.5mL/minDetector : RI(small cell volume)Column temp. : 40˚CReducing the analysis time to less than half of that for the standardcolumn (KF-802.5) requires KF-602.5 which enables high-speed analysis.On the other hand, KF-402.5HQ has a number of theoretical plates 1.5times larger than that of the standard column, thereby improvingresolution especially in the analysis of molecules that have a small tomedium molecular weight. Both columns spend solvent amounting toonly about one third of the quantity spent by the standard column and areeco-friendly.Column : Shodex GPC KF-802.5 x 2Shodex GPC KF-402.5HQ x 2Shodex GPC KF-602.5 x 2Eluent : THFFlow rate : (KF-802.5) 1.0mL/min(KF-402.5HQ) 0.3mL/min(KF-602.5) 0.6mL/minDetector : (KF-802.5) RI(conventional type)(KF-402.5HQ, KF-602.5) RI(small cell volume)Column temp. : 40˚CPhthalatesSample : 10μL1. Dioctylphthalate 0.1%2. Dibutyllphthalate 0.1%3. Dipropylphthalate 0.1%4. Diethylphthalate 0.1%5. Dimethylphthalate 0.1%6. Propylbenzene 0.2%7. Ethylbenzene 0.2%8. Toluene 0.2%9. Benzene 0.2%23456 78 957Organic SEC (GPC) columns : Rapid Analysis, High Performance Analysisnot detected101 2 3 45 6 7 8 9 10 11 12 13 14 15 16min01 2 3 45 6 7 8 9 10 11 12 13 14 15 16min812 16 20 minColumn : Shodex GPC KF-402HQ x 2Eluent : THFFlow rate : 0.3mL/minDetector : UV(210nm), APCI-MS(SIM)Column temp. : 40˚CColumn : Shodex GPC KF-401HQ x 2Eluent : THFFlow rate : 0.3mL/minDetector : UV(254nm) (small cell volume)Column temp. : 40˚C

Standard analysiscolumns58Organic SEC (GPC) columns : Linear calibration typeHigh temperature/Ultra high temperature analysis SEC (GPC) columnsOrganic SEC (GPC) columns : Linear calibration typeFeaturesLF Employs a special packing material with a wide pore size distribution (multi pore type)Enables analysis over a broad range of molecular weights (1 to 2 million)Highly linear calibration curve without inflection pointsMolecular weight distribution can be determined with high precisionColumns for rapid analysis (LF-604) and columns for high performance analysis(LF-404) enabling reduction in solvent use are also availableNotebook No.1 News No.4, 40Standard columnsProduct CodeF6021041F6709621F6021042F6709621F6021043F6709621GPC LF-804GPC LF-GGPC LF-604GPC LF-GGPC LF-404GPC LF-G10 10 2 10 3 10 4 10 5 10 6 10 7FeaturesHT-800UT-800Standard columnsF6208700F6208710F6208720F6208730F6208740F6208770F6709410F6208600F6208610F6208620F6709400F6208390F6700280Product NameRapid analysis downsized columnsGPC HT-803GPC HT-804GPC HT-805GPC HT-806GPC HT-806MGPC HT-807GPC HT-GGPC UT-802.5GPC UT-806MGPC UT-807GPC UT-GGPC AT-806MSGPC AT-GLF-804, 604, 404Plate Number(TP/column)≥ 7,000≥ 7,000≥ 7,000≥ 7,000≥ 7,000≥ 4,000(guard column)≥ 4,400≥ 4,400≥ 3,300(guard column)≥ 6,000(guard column)Plate Number(TP/column)≥ 17,000(guard column)≥ 9,000(guard column)High-performance semi-micro column≥ 14,000(guard column)Molecular weight range with polystyrene (eluent : THF)Product CodeMolecular weight (Polystyrene)Varied product lineup to support a wide range of molecular weightsExclusion Limit *(PS)70,000400,0004,000,000(20,000,000)**(20,000,000)**(200,000,000)**−20,000(20,000,000)**(200,000,000)**−(20,000,000)**−Exclusion Limit Particle Size Maximum Pore Size Column Size(PS)(μm)(Å)(mm) I.D. x L2,000,000−663,000−8.0 × 3004.6 × 10Shipping solvent : tetrahydrofuran(THF)Use of the LF-604 series with semi-micro type devices is recommended.2,000,000−663,000−6.0 × 1504.6 × 10Shipping solvent : tetrahydrofuran(THF)Use of the LF-404 series with semi-micro type devices is recommended.2,000,000−663,000−4.6 × 2504.6 × 10Shipping solvent : tetrahydrofuran(THF)High temperature/Ultra high temperature analysisSEC (GPC) columnsDedicated to SEC analysis at high/ultra high temperatures with a maximum usable temperature of 210˚CSuitable for the analysis of supermolecule-containing samplesProduct NameUsable Temperature(˚C)100−150100−150100−150100−150100−150100−150100−150100−210100−210100−210100−210Ta−150***Ta−150**** The columns with ‘M’ at the end of the column name are mixed-gel columns capable of awide molecular weight distribution range of samples.Molecular weight range with polystyrene (eluent : THF)Particle Size(μm)13131313131813303030301215Maximum Pore Size(Å)5001,5005,00010,00010,00020,000−30010,00020,000−10,000−Column Size(mm) I.D. x L8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 508.0 × 3008.0 × 3008.0 × 3008.0 × 508.0 × 2508.0 × 50Shipping solvent : toluene*Exclusion limit was measured with THF**( )Estimated value*** Ta : ambient temperatureMolecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9Molecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9HT-803HT-804HT-805HT-806HT-807UT-802.5HT-806MUT-806MAT-806MSUT-807*Contact Shodex or our distributors near you for customized columns.

Calibration curve for LF-804 usingPS standardMolecular weight (Polystyrene)10 710 610 510 410 3Calibration curves for LF-604 and LF-404using PS standardMolecular weight (Polystyrene)10 710 610 510 4LF-40410 3LF-604Comparison of polystyrene separationwith different numbers of LF-40445LF-404 x 11LF-404 x 2236712345623Sample :1. PS Mw : 1,030,0002. PS Mw : 152,0003. PS Mw : 66,0004. PS Mw : 22,0005. PS Mw : 5,0506. PS Mw : 5807. Ethylbenzene74567Standard analysiscolumns10 8 Elution volume (mL)10 2 5 6 7 8 9 10 11 1210 21.0 1.5 2.0 2.5 3.0 3.5 4.0LF-404 x 31Elution volume (mL)0510 15 20 25 30 minColumn : Shodex GPC LF-804Eluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CPolyurethane0 5 10 15 20 0 5 10 15 20 25 0 5 10 15 20 25minminminColumn : Shodex GPC LF-404 x 2Eluent : THFFlow rate : 0.3mL/minDetector : RI(small cell volume)Column temp. : 40˚CMolecular weight (Polystyrene)10 610 510 410 3Sample : Polyurethane 0.1%, 20μLCalibration curves for HT-800 seriesusing PSHT-804HT-806HT-803HT-805HT-806MColumn : Shodex GPC LF-604, LF-404Eluent : THFFlow rate : (LF-604) 0.5mL/min(LF-404) 0.3mL/minDetector : RI(small cell volume)Column temp. : 40˚CPolyamide (Nylon6/6)Column : Shodex GPC LF-404Eluent : 5mM CF 3 COONa in HFIPFlow rate : 0.15mL/minDetector : RI(small cell volume)Column temp. : 40˚C15Vo12025Sample : Nylon TM 6/6 0.1%, 20μL2303540 minColumn : Shodex GPC LF-404 x nEluent : THFFlow rate : 0.3mL/minDetector : RI(small cell volume)Column temp. : 40˚CXylanSample : Xylan 0.1%, 100μLColumn : Shodex GPC LF-804Eluent : 20mM H 3 PO 4 + 20mM LiBr in(DMSO/DMF=80/20)Flow rate : 0.6mL/minDetector : RIColumn temp. : 50˚CEffect of the particle size of material packed in high temperature SEC columnsColumn : UT-806M x 3(Partical size : 30μm)Sample :1. Polystyrene(MW : 20,000,000)2. HDPE-AFor the analysis of samples containingmacromolecules, GPC UT columns arerecommended because of their ability toprevent the breakage of molecularchains. High temperature SEC columnsare suitable for the analysis of insolublepolymers, such as polyethylene andpolypropylene.Column : Shodex GPC UT-806MHT-806M, AT-806MSEluent : 0.1% BHT in ODCBFlow rate : 1.0mL/minDetector : RIColumn temp. : 140˚C59Organic SEC (GPC) columns : Linear calibration typeHigh temperature/Ultra high temperature analysis SEC (GPC) columns10 7 Elution volume (mL)10 2Column : HT-806M x 2(Partical size : 13μm)Column : AT-806MS x 2(Partical size : 12μm)105 6 7 8 9 10 11 12 131212Column : Shodex GPC HT-800 seriesEluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.10VoShear degradationShear degradationVo15 2025 min 10 15 25 min

Standard analysiscolumnsOrganic SEC (GPC) columns : HFIP solvent typeFeaturesHFIP-800 Columns exclusively for use with hexafluoroisopropanol (HFIP)HFIP-600Rapid analysis, solvent-saving typeStandard columnsHFIP-800 series60Organic SEC (GPC) columns : HFIP solvent typeProduct CodeF6028530F6028540F6028550F6028560F6028590F6028570F6700500F6021030F6021040F6021050F6021060F6021080F6021070F6700511Product NameGPC HFIP-803GPC HFIP-804GPC HFIP-805GPC HFIP-806GPC HFIP-806MGPC HFIP-807GPC HFIP-LGGPC HFIP-603GPC HFIP-604GPC HFIP-605GPC HFIP-606GPC HFIP-606MGPC HFIP-607GPC HFIP-GPlate Number(TP/column)≥ 12,000≥ 12,000≥ 10,000≥ 10,000≥ 10,000≥ 4,000(guard column)≥ 12,000≥ 12,000≥ 5,000≥ 5,000≥ 6,000≥ 3,000(guard column)Exclusion Limit(PMMA)*30,000100,0001,000,000(10,000,000)**(10,000,000)**(100,000,000)**−* The columns with ‘M’ at the end of the column name are mixed-gel columns capable of awide molecular weight distribution range of samples.Rapid analysis downsized columnsHFIP-600 seriesProduct CodeProduct NamePlate Number(TP/column)Exclusion Limit(PMMA)*30,000100,0001,000,000(10,000,000)**(10,000,000)**(100,000,000)**−ParticleSize(μm)1071010101815ParticleSize(μm)33101010188MaximumPore Size(Å)5001,5005,00010,00010,00020,000−MaximumPore Size(Å)5001,5005,00010,00010,00020,000−Column Size(mm)I.D. x L8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 50Shipping solvent : hexafluoroisopropanol (HFIP)*PMMA : polymethylmethacrylate**( )Estimated valueUse of the HFIP-600 series with semi-micro type devices is recommended.Column Size(mm)I.D. x L6.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1504.6 × 10* The columns with ‘M’ at the end of the column name are mixed-gel columns capable of awide molecular weight distribution range of samples.Shipping solvent : hexafluoroisopropanol (HFIP)*PMMA : polymethylmethacrylate**( )Estimated valueMolecular weight range with PMMA (eluent : HFIP)Molecular weight (PMMA)10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9HEIP-803, 603HEIP-804, 604HEIP-805, 605HEIP-806, 606HEIP-807, 607HEIP-806M, 606M*Contact Shodex or our distributors near you for customized columns.

Calibration curves for HFIP-800 seriesusing polymethylmethacrylate (PMMA)Molecular weight (PMMA)10 7 110 62310 545610 47810 310 29105 6 7 8 9 10 11 12Elution volume (mL)HFIP-805HFIP-806MHFIP-804HFIP-803HFIP-806Calibration curves for HFIP-600 seriesusing polymethylmethacrylate (PMMA)Molecular weight (PMMA)10 610 510 410 310 2HFIP-605HFIP-606MHFIP-604HFIP-603HFIP-6061.0 1.5 2.0 2.5 3.0 3.5 4.0Polyethylene terephthalate (PET)Sample : PET 0.2%, 20μLMw=19,3000 5 1015minStandard analysiscolumnsElution volume (mL)Column : Shodex GPC HFIP-800 seriesEluent : HFIPFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.Polyamide (effect of salt addition)HFIP5mM CF3COONa/HFIP10 15 20 2530 minColumn : Shodex GPC HFIP-600 seriesEluent : 5mM CF 3COONa in HFIPDetector : RI(small cell volume)Column temp. : 40˚CSample : Polycaprolactum (Nylon TM 6)In SEC analysis using HFIP some samplesmay yield abnormal peaks as a result ofelectrostatic interaction. In this case, theimpact of electrostatic interaction can beremoved by adding sodium trifluoroacetateto HFIP.Column : Shodex GPC HFIP-606M x 2Eluent : 5mM CF 3COONa in HFIPFlow rate : 0.6mL/minDetector : RI(small cell volume)Column temp. : 40˚CPolybutylene terephthalate (PBT)Sample : PBT 0.05%, 500μL1520 30 min61Organic SEC (GPC) columns : HFIP solvent typeColumn : Shodex GPC HFIP-806M x 2Eluent : HFIP(solid line), 5mM CF 3 COONa in HFIP(broken line)Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex GPC HFIP-805 + HFIP-803Eluent : 5mM CF 3 COONa in HFIPFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CPolyamides (Nylon 6/10 and Nylon 6)PolyacetalSample : Nylon TM 6/10 0.2%, 20μL Sample : Nylon TM 6 0.2%, 20μL Sample : Polyacetal 0.2%, 20μLMw=29,300 Mw=38,100 Mw=176,0000 5 1015min0 5 10150 5 10 15minminColumn : Shodex GPC HFIP-606M x 2Eluent : 5mM CF 3 COONa in HFIPFlow rate : 0.6mL/minDetector : RI(small cell volume)Column temp. : 40˚CColumn : Shodex GPC HFIP-606M x 2Eluent : 5mM CF 3 COONa in HFIPFlow rate : 0.6mL/minDetector : RI(small cell volume)Column temp. : 40˚C

InformationApplicability of SEC (GPC) columns to solventreplacementColumn NameShipping Solvent : THFShipping Solvent : DMFSolventKF-801KF-802KF-802.5KF-803LKF-804LKF-803KF-804KF-805KF-806KF-807KF-806MKF-805LKF-806LKF-807LKF-601KF-602KF-602.5KF-603KF-604KF-605KF-606KF-607KF-606MLF-804LF-604LF-404KD-801KD-802KD-802.5KD-803KD-804KD-805KD-806KD-807KD-806M62Applicability of SEC (GPC) columns to solvent replacementTHFChloroformCarbon tetrachlorideBenzeneToluenep-Xyleneo-Dichlorobenzene(ODCB)Trichlorobenzene(TCB)DioxaneDiethyl etherEthyl acetateAcetoneMethyl ethyl ketoneDimethylformamide(DMF)Shipping Solvent : ChloroformK-801 K-802 K-803 K-804K-802.5K-805K-803LK-806K-804LK-807K-806MK-805LK-806LK-807LShipping Solvent : THFKF-401HQ KF-403HQKF-402HQ KF-404HQKF-402.5HQ KF-405LHQKF-406LHQ*1*1*1Dimethylacetamide(DMAc)*1*1*1Hexafluoroisopropanol(HFIP)*1*1m-Cresolo-ChlorophenolQuinolinN-Methylpyrrolidone(NMP)*1*1*1Dimethylsulfoxide(DMSO)*1*1*130% m-Cresol/Chloroform30% o-Chlorophenol/Chloroform30% HFIP/ChloroformHexaneAcetonitrileMethanolWater: Solvent replacement possible: Solvent replacement possible, but this may cause column performance to slightly deteriorate*1 : Usable at 40˚C or higher: Solvent replacement not possible

Calibration Standards for SEC[Polystyrene (PS)]FeaturesFor organic solvent SEC (GPC)Less branched polystyrene with anionic polymerizationEasily soluble in tetrahydrofuran (THF), chloroform, toluene, and o-dichlorobenzeneKit typeProduct CodeF8601105Product Name Contents MW RangeSTANDARD SL-1050.5g x 10 kinds500~22,000Structural formula of S seriesC 4H 9 CH 2 CH HCalibration standardsfor SECF8602105STANDARD SM-1050.5g x 10 kinds1,200~3,800,000nF8603075STANDARD SH-750.5g x 7 kinds590,000~7,500,000SL-105 SM-105 SH-105Std. No. Mp Mw/Mn Std. No. Mp Mw/Mn Std. No. Mp Mw/MnS-20S-11S-6.9S-5.0S-4.4S-2.9S-1.9S-1.2S-1.0S-0.519,80010,7006,9305,0304,4302,9001,9301,2001,0505801.021.021.031.031.031.031.051.071.071.12S-3730S-2480S-1230S-579S-197S-55.1S-31.4S-12.8S-3.95S-1.20[Polymethylmethacrylate (PMMA)]Features3,730,0002,480,0001,230,000579,000197,00055,10031,40012,8003,9501,200F8604075 STANDARD M-75 0.5g x 7 kinds1.041.051.051.021.021.031.021.021.031.07S-7450S-3790S-3250S-2220S-1820S-1060S-5917,450,0003,790,0003,250,0002,220,0001,820,0001,060,000591,000For organic solvent SEC (GPC)Narrow molecular weight distribution rangeEasily soluble in hexafluoroisopropanol (HFIP) and dimethylformamide (DMF)Kit typeProduct Code[Pullulan]FeaturesProduct Name Contents MW Range1,800~950,000(Note)Molecular weights (Mp, Mw/Mn) of eachkit may vary depending on production lots.For aqueous SEC (GFC)Unbranched pullulan standardHigh solubility in water eliminates the possibility of recrystallization1.071.051.041.031.041.031.03(Note)Molecular weights (Mp, Mw/Mn)of each kit may vary dependingon production lots.Std. No. Mp Mw/MnM-949M-451M-139M-53M-21M-7.1M-1.9949,000451,000139,00052,60020,8007,1001,8901.051.021.051.021.031.081.1063Calibration Standards for SECKit typeProduct CodeF8400000 STANDARD P-82 0.2g x 8 kindsSingle typeProduct CodeF8402500F8401300F8400800F8400400F8400200F8400100F8400050F8400020F8400010F8400005F8400003F8400002F8400001Product NameProduct NameNEW STD P-2500NEW STD P-1300STD P-800STD P-400STD P-200STD P-100STD P-50STD P-20STD P-10STD P-5STD P-3STD P-2STD P-1Contents0.2g0.2g0.5g0.5g0.5g0.5g0.5g0.5g0.5g0.5g0.2g0.2g0.2gContentsMp2,350,0001,220,000708,000375,000200,000107,00047,10021,10011,1005,9002,8902,1501,420MW Range5,000~800,000Mw/Mn1.491.371.231.131.131.121.061.071.101.091.121.111.18Std. No.P-800P-400P-200P-100P-50P-20P-10P-5(Note)Molecular weights (Mp, Mw/Mn)of each kit may vary dependingon production lots.Structural formula of P seriesO CH 2 H HCH 2OHH HCH 2OHHHHOHOHOOHOHH O OH H O OH HH OHH OHH OHn

Application fields64Pharmaceuticals and Cosmetics analysisPharmaceuticals, MetabolitesEmulsifiersProduct Name Separation mode PageProduct Name Separation mode PageODP2 HPET-RP1RSpak DE seriesRSpak DS seriesAsahipak ODP-40Asahipak ODP-50Asahipak C8P-50Asahipak C4P-50Silica C18MSilica C18PODSpak F-411Asahipak NH2P seriesRSpak NN seriesUSPpak MN-431Asahipak GS-320 HQ SEC+RPC 48Product Name Separation mode PageMSpak GS-320SEC+RPC 48ORpak CDA-453 HQ CS22MSpak PKSEC+RPC 6ORpak CDB-453 HQ CS22MoisturizersRPCRPCRPCRPCRPCRPCRPCRPCPharmaceuticals & CosmeticsColumn list for Pharmaceuticals and Cosmetics analysis11111111Asahipak GF-310 HQMSpak GF-310GPC KF-802GPC KF-402HQSEC+RPCSEC+RPCSECSEC5050525610101010RPC20Product Name Separation mode PageRPC20RSpak DE seriesRPC11RPC20RSpak DS seriesRPC11HILIC13Asahipak ODP-40RPC10IEC+RPC 12Asahipak ODP-50RPC10LEX+SEC 32Silica C18MRPC20Silica C18PRPC20[Substances in bio-fluid]ODSpak F-411RPC20Product Name Separation mode PageODP2 HPRPC11Asahipak GF-310 HQ SEC+RPC 50MSpak GF-310SEC+RPC 50[Polyalcohols]PreservativesOptical active materialsORpak CDC-453 HQORpak CDBS-453ORpak CRX-853AFpak ABA-894CSCSCSCS22222222, 28Product NameSeparation modePageSUGAR SC1211LEX+HILIC33SUGAR SC1011LEX+SEC32OHpak SB-802.5 HQSEC46SB402.5SEC46Asahipak GF-310 HQSEC50MSpak GF-310SEC50RSpak DE seriesRPC11[Protein hydrolysates]Product NameSeparation modePagePROTEIN KW-802.5SEC44KW402.5SEC44Asahipak ODP-40RPC10Asahipak ODP-50RPC10Separation Mode[Mucopolysaccharides]Product Name Separation modeOHpak SB-800 HQ series SECSB400 seriesSECPage4646RPC : Reversed phase chromatographyHILIC : Hydrophilic interaction chromatographyIEC : Ion exchange chromatographyLEX : Ligand exchange chromatographySEC : Size exclusion chromatographyCS : Chiral Separation

FoodsNutritional ingredientsColumn list for Foods analysis[Monosaccharides, Disaccharides, Sugar alcohols] [Fatty acids]Product Name Separation mode PageProduct NameSeparation modePageApplication fieldsSUGAR SC1011SUGAR SC1821SUGAR SP0810SUGAR KS-801SUGAR KS-802SUGAR SC1211SUGAR SZ5532RSpak DC-613Asahipak NH2P seriesLEX+SECLEX+SECLEX+SECLEX+SECLEX+SECLEX+HILICLEX+HILICLEX+HILICHILIC323232323233333313RSpak DE seriesRSpak RP18 seriesSilica C18MSilica C18PSilica 5SILAsaipak GF-310 HQMSpak GF-310GPC KF-802GPC KF-402HQRPCRPCRPCRPCRPCSECSECSECSEC111120202050505256[Oligosaccharides]Product NameSeparation modePage[“Umami”, Nucleic acids]Product Name Separation modePage65Asahipak GS-220 HQ SECOHpak SB-802 HQSECOHpak SB-802 5HQSECSUGAR KS-802SECRSpak DC-613LEX+HILICAsahipak NH2P series HILIC[Low-molecular water-soluble fiber]484646323313Asahipak GS-320 7E[Amino acids]Product NameCXpak P-421SRSpak NN seriesIEC+SECSeparation modeIECIEC+IEX+RPC48Page2612Foods analysisProduct NameAsahipak GS-220 HQSeparation modeSECPage48[Polysaccharides]Product NameOHpak SB-800 HQ seriesSUGAR KS-800 seriesSeparation modeSECSECPage4632Food safety[Food additives]Product NameSeparation modePage[Organic acids]Product Name Separation modeSUGAR SH1011IEX+RPCSUGAR SH1821IEX+RPCRSpak KC-811IEX+RPCRSpak NN seriesIEX+RPCIC SI-90 4EICIC SI-50 4EICIC SI-52 4EICODP2 HPRPCRSpak DE seriesRPC[Water soluble vitamins]Page363636123838381111ET-RP1RSpak DE seriesRSpak DS seriesAsahipak ODP-40Asahipak ODP-50Asahipak NH2P series[Pesticides]Product NameRSpak GOLF-413RSpak CARB seriesRSpak DE-413IC SI-90 4ERSpak NN seriesRPCRPCRPCRPCRPCHILICSeparation modeRPCRPCRPCICIEC+RPC111111101013Page1212113812Product NameODP2 HPRSpak DE seriesAsahipak ODP-40Asahipak ODP-50Asahipak NH2P seriesRSpak NN seriesSeparation modeRPCRPCRPCRPCHILICIEC+RPCPage111110101312[Pretreatment of residual pesticides]Product NameCLNpak EV seriesSeparation modeSEC(GPC clean-up)Page6[Fat-soluble vitamins]Product NameAsahipak ODP-40Asahipak ODP-50Silica 5SILGPC KF-801GPC KF-401HQSeparation modeRPCRPCNPCSECSECPage1010205256Separation ModeRPC : Reversed phase chromatographyNPC : Normal phase chromatographyHILIC : Hydrophilic interaction chromatographyIEC : Ion exchange chromatographyLEX : Ligand exchange chromatographyIEX : Ion exclusion chromatographyIC : Ion chromatographySEC : Size exclusion chromatography

Application fields66Biotechnology analysisProduct NameAsahipak GS-320 HQMSpak GS-320RSpak NN seriesAXpak WA-624RSpak DE seriesAFpak AED-894AFpak AIA-894AFpak APB-894[Oligo nucleic acids]Product NameAsahipak GS-320 HQMSpak GS-320Asahipak ES-502N 7CIEC DEAE3N-4TRSpak DE series[DNA, RNA]Product NameOHpak SB-800 HQ seriesSB400 seriesAsahipak GF seriesAFpak AAF-894AFpak ALS-894[Amines]Product NameODP2 HPRSpak DE seriesRSpak DS seriesAsahipak ODP-40Asahipak ODP-50Asahipak C8P-50Asahipak C4P-50Asahipak ES-502C 7CColumn list for Biotechnology analysisGenomicsProteomics[Nucleobases, Nucleotides, Nucleosides][Amino acids]HormonesBiotechnologySeparation mode PageProduct Name Separation mode PageIEC+SEC 48CXpak P-421SIEC26IEC+SEC 48IC YS-50IEC40IEC+RPC 12RSpak NN seriesIEC+IEX+RPC 12IEC24Asahipak GS-320 HQ IEC+SEC 48RPC11MSpak GS-320IEC+SEC 48AFC28Asahipak ODP-50RPC10AFC28AFC28[Peptides, Proteins]Product Name Separation mode PagePROTEIN KW-800 series SEC40Separation mode PageKW400 seriesSEC44IEC+SEC 48Asahipak GF-HQ series SEC50IEC+SEC 48Asahipak GS-HQ series SEC48IEC24IEC SP-825IEC26IEC24IEC CM-825IEC26RPC11IEC QA-825IEC24IEC DEAE-825IEC24Separation mode PageAsahipak ES-502C 7C IEC26SEC46Asahipak ES-502N 7C IEC24SEC46IEC SP-420NIEC26SEC50IEC DEAE3N-4TIEC24AFC28PIKESS SP-2BIEC26AFC28PIKESS DEAE-2BIEC24RSpak RP18-415RPC11Asahipak ODP-40RPC10Asahipak ODP-50RPC10Asahipak C8P-50RPC10Asahipak C4P-50RPC10HIC PH-814HIC26Separation mode PageAFpak APH-894AFC28RPC11AFpak AAB-894AFC28RPC11AFpak AAP-894AFC28RPC11AFpak ALS-894AFC28RPC10AFpak AST-894AFC28RPC10RPC10RPC10IEC26[Steroids]Product NameSeparation modePageAsahipak ODP-40Asahipak ODP-50Asahipak NH2P seriesOHpak SB-802.5 HQSB402.5Asahipak GF-310 HQMSpak GF-310RPCRPCHILICSECSECSECSEC11111346465050Separation ModeRPC : Reversed phase chromatographyNPC : Normal phase chromatographyHILIC : Hydrophilic interaction chromatographyIEC : Ion exchange chromatographyHIC : Hydrophobic interaction chromatographyAFC : Affinity chromatographyLEX : Ligand exchange chromatographyIEX : Ion exclusion chromatographySEC : Size exclusion chromatography

BiotechnologyColumn list for Biotechnology analysisGlycomicsLipids[Glycoproteins][Phospholipids]Product Name Separation mode PageProduct Name Separation modePageApplication fieldsPROTEIN KW-800 seriesSEC44Silica 5SILNPC20KW400 seriesSEC44Asahipak GF-310 HQSEC50Asahipak GF-HQ seriesSEC50MSpak GF-310SEC50Asahipak GS-HQ seriesSEC48GPC KF-802SEC52IEC SP-825IEC26GPC KF-402HQSEC56IEC CM-825IEC26IEC QA-825IEC24[Lipoproteins]IEC DEAE-825Asahipak ES-502C 7CAsahipak ES-502N 7CIEC SP-420NIEC DEAE3N-4TPIKESS SP-2BPIKESS DEAE-2BRSpak RP18-415Asahipak ODP-40Asahipak ODP-50Asahipak C8P-50IECIECIECIECIECIECIECRPCRPCRPCRPC2426242624262411101010Product NameOHpak SB-805 HQSB405AFpak AHR-894AFpak ADS-894Separation modeSECSECAFCAFCPage4646282867Biotechnology analysisAsahipak C4P-50RPC10HIC PH-814HIC26AFpak ACA-894AFC28AFpak AGA-894AFC28AFpak ALC-894AFC28AFpak ARC-894AFC28[Sugar chains]Product NameSeparation modePageAsahipak NH2P seriesHILIC13AFpak seriesAFC28[Monosaccharides, Disaccharides, Sugar alcohols]Product Name Separation mode PageSUGAR SC1011LEX+SEC 32SUGAR SC1821LEX+SEC 32SUGAR SP0810LEX+SEC 32SUGAR KS-801LEX+SEC 32SUGAR KS-802LEX+SEC 32SUGAR SC1211LEX+HILIC 33SUGAR SZ5532LEX+HILIC 33RSpak DC-613LEX+HILIC 33Asahipak NH2P series HILIC13[Sialic acid, Uronic acid, Aldonic acid]Product Name Separation mode PageSUGAR SH1011IEX+SEC 36SUGAR SH1821IEX+SEC 36Separation ModeRPC : Reversed phase chromatographyNPC : Normal phase chromatographyHILIC : Hydrophilic interaction chromatographyIEC : Ion exchange chromatographyHIC : Hydrophobic interaction chromatographyAFC : Affinity chromatographyLEX : Ligand exchange chromatographyIEX : Ion exclusion chromatographySEC : Size exclusion chromatography

Application fields Environment analysis68Product NameRSpak KC-811 6E[Cations]Product NameIC YS-50IC YK-421[Surfactants]Product NameAsahipak GF-310 HQMSpak GF-310Silica C18M[Pesticides]Product NameRSpak GOLF-413RSpak CARB seriesIC SI-90 4ERSpak NN seriesColumn list for Environment analysisWater qualitySoilEnvironment[Anions][Anions]Product Name Separation mode PageProduct Name Separation mode PageIC SI seriesIC I-524AIC NI-424ICICIC383838IC SI seriesIC I-524AIC NI-424ICICIC383838[Oxyhalides][Heavy metals]Product Name Separation mode PageProduct Name Separation mode PageIC SI-91 4CIC38IC T-521IC40IC SI-52 4EIC38IC Y-421IC40[Cyanide, Cyanogen chloride]Separation mode[Rare earth elements]PageProduct Name Separation mode PageIEX36IC R-621IC40[Humic substances]Separation mode PageProduct Name Separation mode PageIC40OHpak SB-805 HQSEC46IC40SB405SEC46[Organic arsenics]Separation mode PageProduct Name Separation mode PageSEC+RPC 50RSpak NN seriesIEC+RPC 12SEC+RPC 50RPC20[Pesticides]Product Name Separation mode PageRSpak GOLF-413RPC12Separation mode PageRSpak CARB series RPC12RPC12IC SI-90 4EIC38RPC12RSpak NN seriesIEC+RPC 12IC38IEC+RPC 12Environmental hormonesPretreatment of phthalates, PCBs,Benzo [a] pyreneProduct NameCLNpak PAE seriesBiodiesel[Cations]Product NameIC YS-50[Fatty acid glycerides]Product NameAsahipak GF-310 HQMSpak GF-310[Fatty acid methyl esters]Product NameRSpak DS series[Organic acids]Product NameIC SI-52 4ESeparation modeSEC(GPC clean-up)Separation modeICSeparation modeSECSECSeparation modeRPCSeparation modeICPage6Page40Page5050Page11Page38Bioethanol[Monosaccharides, Oligosaccharides]Product Name Separation mode PageSUGAR SP0810Asahipak NH2P seriesLEX+SECHILIC3213[Oligosaccharides, Alcohols]Product Name Separation mode PageSUGAR KS-802SUGAR SC1821LEX+SECLEX+SEC3232[Saccharides, Organic acids, Alcohols]Product Name Separation mode PageSUGAR SH1011SUGAR SH1821IEX+RPC+SECIEX+RPC+SEC3638[Hemicellulose, Cellulose]Product Name Separation mode PageGPC LF-804GPC KD-800 seriesSECSEC5854Separation ModeRPC : Reversed phase chromatographyHILIC : Hydrophilic interaction chromatographyIEC : Ion exchange chromatographyLEX : Ligand exchange chromatographyIEX : Ion exclusion chromatographyIC : Ion chromatographySEC : Size exclusion chromatography

USP(United States Pharmacopeia) Column ListNo. Packing material Recommended Column PageL1L3L7L8L10L11L17L19L20L21L22L23L25L26L33L34L37L38L39L45L58L59L67L71Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 1.5 to10μm in diameter, or a monolithic rodPorous silica particles, 1.5 to 10μm in diameter, or a monolithic silica rodOctylsilane chemically bonded to totally porous silica particles, 1.5 to 10μm in diameter,or a monolithic silica rodAn essentially monomolecular layer of aminopropylsilane chemically bonded to totallyporous silica gel support, 3 to 10μm in diameterNitrile groups chemically bonded to porous silica particles, 3 to 10μm in diameterPhenyl groups chemically bonded to porous silica particles, 1.5 to 10μm in diameterStrong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzenecopolymer in the hydrogen form, 7 to 11μm in diameterStrong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzenecopolymer in the calcium form, about 9μm in diameterDihydroxypropane groups chemically bonded to porous silica particles, 3 to 10μm indiameterA rigid, spherical styrene-divinylbenzene copolymer, 3 to 10μm in diameterA cation-exchange resin made of porous polystyrene gel with sulfonic acid groups,about 10μm in sizeAn anion-exchange resin made of porous polymethacrylate or polyacrylate gel withquartenary ammonium groups, about 10μm in sizePacking having the capacity to separate compounds with a molecular weight range from100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationicwater-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylatedether (surface contained some residual carboxyl functional groups) was found suitableButyl silane chemically bonded to totally porous silica particles, 1.5 to 10μm in diameterPacking having the capacity to separate dextrans by molecular size over a range of 4,000to 500,000 Da. It is spherical, silica-based, and processed to provide pH stabilityStrong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzenecopolymer in the lead form, about 9μm in diameterPacking having the capacity to separate proteins by molecular size over a range of 2,000to 40,000 Da. It is a polymethacrylate gelA methacrylate-based size-exclusion packing for water-soluble samplesA hydrophilic polyhydroxymethacrylate gel of totally porous spherical resinBeta cyclodextrin bonded to porous silica particles, 5 to 10μm in diameterStrong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzenecopolymer in the sodium form, about 6 to 30μm diameterPacking having the capacity to separate proteins by molecular weight over the range of 5to 7000kDa. It is spherical (5 - 10μm), silica-based, and processed to provide hydrophiliccharacteristics and pH stabilityPorous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group ofthe polymer, 2 to 10μm in diameterA rigid, spherical polymetacrylate, 4 to 6μm in diameterSilica C18MSilica C18PODSpak F-411C18Silica 5SILSilica 5C8Silica 5NHSilica 5CNSilica 5NPESUGAR SH1011SUGAR SH1821RSpak KC-811IC Y-521SUGAR SC1011SUGAR SC1821SUGAR SC1211USPpak MN-431PROTEIN KW-800 seriesKW400 seriesGPC KF,K,KD,HFIP,LF,AT,UT seriesRSpak DS-613RSpak DS-413RSpak RP18-415RSpak RP18-413RSpak RP18-613CXpak P-421SSUGAR SP0810SUGAR SC1011SUGAR SC1821SUGAR KS-800 seriesSUGAR SC1211SUGAR SZ5532USPpak MN-431RSpak DC-613SUGAR SH1011SUGAR SH1821RSpak KC-811IC Y-521IEC QA-825OHpak SB-802 HQOHpak SB-802.5 HQSB402.5Silica 5C4PROTEIN KW-800 seriesKW400 seriesSUGAR SP0810OHpak SB-803 HQSB403OHpak SB-800 HQ seriesSB400 seriesOHpak SB-800 HQ seriesSB400 seriesODP2 HPRSpak DM-614ORpak CDBS-453CXpak P-421SSUGAR KS-800 seriesRSpak DC-613PROTEIN KW-800 seriesKW400 seriesAsahipak ODP-40Asahipak ODP-50ET-RP1RSpak DE-613RSpak DE-413RSpak DE-2132020202020202020203636364032323332444452,54,56,58,6011111111112632323232333332333636364024464646204444324646464646461111222632334444101011111111Application fields69USP(United States Pharmacopeia) Column List

Semi-micro andmicro columnsSemi-microMicro Columns70Polymer-based Packed Columns for Reversed Phase and Hydrophilic Interaction Chromatography (HILIC)Semi-microMicro ColumnsPolymer-based Packed Columns for Reversed Phase and Hydrophilic Interaction Chromatography (HILIC)Asahipak ODP semi-micro and micro typeI.D.2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)25015050352501505035250150503525015050352501505035* See page 10 for Asahipak ODP-40.* See page 82 for preparative columns.* See page 12 for RSpak NN-414.Semi-micro and micro columnsBase Material : Polyvinyl alcoholFunctional Group : OctadecylProduct NameODP40-2EODP40-2DODP40-2BODP40-2TODP40-1EODP40-1DODP40-1BODP40-1TODP40-M8EODP40-M8DODP40-M8BODP40-M8TODP40-M5EODP40-M5DODP40-M5BODP40-M5TODP40-M3EODP40-M3DODP40-M3BODP40-M3TProduct CodeF7838021F7838022F7838023F7838024F7838031F7838032F7838033F7838034F7838041F7838042F7838043F7838044F7838051F7838052F7838053F7838054F7838061F7838062F7838063F7838064RSpak NN semi-micro and micro typeBase MaterialFunctional Group : SulfoLengthI.D.(mm)1502.0mm 50351501.0mm 50351500.8mm 50351500.5mm 50351500.3mm 5035: PolyhydroxymethacrylateProduct NameNN414-2DNN414-2BNN414-2TNN414-1DNN414-1BNN414-1TNN414-M8DNN414-M8BNN414-M8TNN414-M5DNN414-M5BNN414-M5TNN414-M3DNN414-M3BNN414-M3TProduct CodeF7860122F7860123F7860124F7860132F7860133F7860134F7860142F7860143F7860144F7860152F7860153F7860154F7860162F7860163F7860164RSpak DE semi-micro and micro typeBase Material : PolymethacrylateI.D.2.0mm1.0mm0.8mm0.5mm0.3mm* See page 11 for RSpak DE-413 series.* See page 82 for preparative columns.2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)2501505035250150503515050351505035150503525015050352501505035250150503525015050352501505035Product NameDE413-2EDE413-2DDE413-2BDE413-2TDE413-1EDE413-1DDE413-1BDE413-1TDE413-M8DDE413-M8BDE413-M8TDE413-M5DDE413-M5BDE413-M5TDE413-M3DDE413-M3BDE413-M3TBase Material : Polyvinyl alcoholFunctional Group : AminoLengthI.D.(mm)Product NameNH2P40-2ENH2P40-2DNH2P40-2BNH2P40-2TNH2P40-1ENH2P40-1DNH2P40-1BNH2P40-1TNH2P40-M8ENH2P40-M8DNH2P40-M8BNH2P40-M8TNH2P40-M5ENH2P40-M5DNH2P40-M5BNH2P40-M5TNH2P40-M3ENH2P40-M3DNH2P40-M3BNH2P40-M3TProduct CodeF7840121F7840122F7840123F7840124F7840131F7840132F7840133F7840134F7840142F7840143F7840144F7840152F7840153F7840154F7840162F7840163F7840164Asahipak NH2P semi-micro and micro typeProduct CodeF7858021F7858022F7858023F7858024F7858031F7858032F7805033F7805034F7858041F7858042F7858043F7858044F7858051F7858052F7858053F7858054F7858061F7858062F7858063F7858064* See page 13 for Asahipak NH2P-40 series.* See page 83 for preparative columns.

I.D.2.0mm1.0mm0.8mm0.5mm0.3mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)1505035150503515050351505035150503550355035503550355035* See page 24 for IEC DEAE3N-4T.Columns for Anion Exchange ChromatographyIEC QA-825 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : Quaternary ammoniumProduct NameQA8-2DQA8-2BQA8-2TQA8-1DQA8-1BQA8-1TQA8-M8DQA8-M8BQA8-M8TQA8-M5DQA8-M5BQA8-M5TQA8-M3DQA8-M3BQA8-M3T* See page 24 for IEC QA-825.* See page 84 for preparative columns.Base Material : PolyhydroxymethacrylateFunctional Group : DiethylaminoethylLengthI.D.(mm)Product NameDEAE3N-2BDEAE3N-2TDEAE3N-1BDEAE3N-1TDEAE3N-M8BDEAE3N-M8TDEAE3N-M5BDEAE3N-M5TDEAE3N-M3BDEAE3N-M3TProduct CodeF7940322F7940323F7940324F7940332F7940333F7940334F7940342F7940343F7940344F7940352F7940353F7940354F7940362F7940363F7940364IEC DEAE3N semi-micro and micro typeProduct CodeF7950223F7950224F7950233F7950234F7950243F7950244F7950253F7950254F7950263F7950264Semi-micro and micro columnsIEC DEAE-825 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : DiethylaminoethylI.D.2.0mm1.0mm0.8mm0.5mm0.3mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)1505035150503515050351505035150503515050351505035150503515050351505035Product NameDEAE8-2DDEAE8-2BDEAE8-2TDEAE8-1DDEAE8-1BDEAE8-1TDEAE8-M8DDEAE8-M8BDEAE8-M8TDEAE8-M5DDEAE8-M5BDEAE8-M5TDEAE8-M3DDEAE8-M3BDEAE8-M3T* See page 24 for IEC DEAE-825.* See page 84 for preparative columns.Base Material : Polyvinyl alcoholFunctional Group : DiethylaminoethylLengthI.D.(mm)Product NameDEAE9A-2DDEAE9A-2BDEAE9A-2TDEAE9A-1DDEAE9A-1BDEAE9A-1TDEAE9A-M8DDEAE9A-M8BDEAE9A-M8TDEAE9A-M5DDEAE9A-M5BDEAE9A-M5TDEAE9A-M3DDEAE9A-M3BDEAE9A-M3TSemi-microMicro ColumnsProduct CodeF7940422F7940423F7940424F7940432F7940433F7940434F7940442F7940443F7940444F7940452F7940453F7940454F7940462F7940463F7940464Asahipak ES-502N semi-micro and micro typeProduct CodeF7960122F7960123F7960124F7960132F7960133F7960134F7960142F7960143F7960144F7960152F7960153F7960154F7960162F7960163F7960164Semi-micro andmicro columnsSemi-microMicro Columns71Columns for Anion Exchange Chromatography* See page 24 for Asahipak ES-502N 7C.* See page 84 for preparative columns.

Semi-micro andmicro columnsSemi-microMicro Columns72Columns for Cation Exchange ChromatographySemi-microMicro ColumnsI.D.2.0mm1.0mm0.8mm0.5mm0.3mmSemi-micro and micro columnsLength(mm)15050351505035150503515050351505035Columns for Cation Exchange ChromatographyIEC SP-825 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : SulfopropylProduct NameSP8-2DSP8-2BSP8-2TSP8-1DSP8-1BSP8-1TSP8-M8DSP8-M8BSP8-M8TSP8-M5DSP8-M5BSP8-M5TSP8-M3DSP8-M3BSP8-M3T* See page 26 for IECSP-825.* See page 84 for preparative columns.Product CodeF7940122F7940123F7940124F7940132F7940133F7940134F7940142F7940143F7940144F7940152F7940153F7940154F7940162F7940163F7940164IEC CM-825 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : CarboxymethylI.D.2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)15050351505035150503515050351505035Product NameCM8-2DCM8-2BCM8-2TCM8-1DCM8-1BCM8-1TCM8-M8DCM8-M8BCM8-M8TCM8-M5DCM8-M5BCM8-M5TCM8-M3DCM8-M3BCM8-M3TProduct CodeF7940222F7940223F7940224F7940232F7940233F7940234F7940242F7940243F7940244F7940252F7940253F7940254F7940262F7940263F7940264IEC SP-420N semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : SulfopropylI.D.2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)50355035503550355035* See page 26 for IEC SP-420N.Product NameSP3N-2BSP3N-2TSP3N-1BSP3N-1TSP3N-M8BSP3N-M8TSP3N-M5BSP3N-M5TSP3N-M3BSP3N-M3TBase Material : Polyvinyl alcoholFunctional Group : CarboxymethylI.D.Length(mm)Product Name2.0mm1.0mm0.8mm0.5mm0.3mm15050351505035150503515050351505035CM9A-2DCM9A-2BCM9A-2TCM9A-1DCM9A-1BCM9A-1TCM9A-M8DCM9A-M8BCM9A-M8TCM9A-M5DCM9A-M5BCM9A-M5TCM9A-M3DCM9A-M3BCM9A-M3TProduct CodeF7950123F7950124F7950133F7950134F7950143F7950144F7950153F7950154F7950163F7950164Asahipak ES-502C semi-micro and micro typeProduct CodeF7960222F7960223F7960224F7960232F7960233F7960234F7960242F7960243F7960244F7960252F7960253F7960254F7960262F7960263F7960264* See page 26 for IEC CM-825.* See page 84 for preparative columns.* See page 26 for Asahipak ES-502C 7C.* See page 84 for preparative columns.

AFpak AAB-894 semi-micro and micro typeLigand : AminobezamidineLengthI.D.(mm)Product Name2.0mm1.0mm0.8mm0.5mm0.3mm50355035503550355035* See page 28 for AFpak AAB-894.Ligand : 5'AMPLengthI.D.(mm)2.0mm1.0mm0.8mm0.5mm0.3mm50355035503550355035AAB-2BAAB-2TAAB-1BAAB-1TAAB-M8BAAB-M8TAAB-M5BAAB-M5TAAB-M3BAAB-M3TProduct NameAAM-2BAAM-2TAAM-1BAAM-1TAAM-M8BAAM-M8TAAM-M5BAAM-M5TAAM-M3BAAM-M3TSemi-micro and micro columnsColumns for Affinity ChromotographyProduct CodeF7970123F7970124F7970133F7970134F7970143F7970144F7970153F7970154F7970163F7970164AFpak AAM-894 semi-micro and micro typeProduct CodeF7970323F7970324F7970333F7970334F7970343F7970344F7970353F7970354F7970363F7970364Ligand : AcriflavineI.D.Length(mm)Product Name2.0mm50AAF-2B35AAF-2T1.0mm5035AAF-1BAAF-1T0.8mm0.5mm0.3mm505050AAF-M8BAAF-M5BAAF-M3B353535AAF-M8TAAF-M5TAAF-M3T* See page 28 for AFpak AAF-894.Semi-microMicro ColumnsBase Material : PolyhydroxymethacrylateAFpak AAF-894 semi-micro and micro typeLigand : AprotininLengthI.D.(mm)2.0mm1.0mm0.8mm0.5mm0.3mm50355035503550355035Product NameAAP-2BAAP-2TAAP-1BAAP-1TAAP-M8BAAP-M8TAAP-M5BAAP-M5TAAP-M3BAAP-M3TProduct CodeF7970223F7970224F7970233F7970234F7970243F7970244F7970253F7970254F7970263F7970264AFpak AAP-894 semi-micro and micro typeProduct CodeF7970423F7970424F7970433F7970434F7970443F7970444F7970453F7970454F7970463F7970464Semi-micro andmicro columnsSemi-microMicro Columns73Columns for Affinity Chromotography* See page 28 for AFpak AAM-894.AFpak AAV-894 semi-micro and micro typeLigand : Avidin* See page 28 for AFpak AAP-894.AFpak ABA-894 semi-micro and micro typeLigand : Bovine serum albuminI.D.Length(mm)Product NameProduct CodeI.D.Length(mm)Product NameProduct Code2.0mm5035AAV-2BAAV-2TF7970523F79705242.0mm5035ABA-2BABA-2TF7970623F79706241.0mm5035AAV-1BAAV-1TF7970533F79705341.0mm5035ABA-1BABA-1TF7970633F79706340.8mm5035AAV-M8BAAV-M8TF7970543F79705440.8mm5035ABA-M8BABA-M8TF7970643F79706440.5mm5035AAV-M5BAAV-M5TF7970553F79705540.5mm5035ABA-M5BABA-M5TF7970653F79706540.3mm5035AAV-M3BAAV-M3TF7970563F79705640.3mm5035ABA-M3BABA-M3TF7970663F7970664* See page 28 for AFpak AAV-894.AFpak ABT-894 semi-micro and micro typeLigand : Biotin* See page 22,28 for AFpak ABA-894.AFpak ACA-894 semi-micro and micro typeLigand : Concanavalin AI.D.Length(mm)Product NameProduct CodeI.D.Length(mm)Product NameProduct Code2.0mm5035ABT-2BABT-2TF7970723F79707242.0mm5035ACA-2BACA-2TF7970823F79708241.0mm5035ABT-1BABT-1TF7970733F79707341.0mm5035ACA-1BACA-1TF7970833F79708340.8mm5035ABT-M8BABT-M8TF7970743F79707440.8mm5035ACA-M8BACA-M8TF7970843F79708440.5mm5035ABT-M5BABT-M5TF7970753F79707540.5mm5035ACA-M5BACA-M5TF7970853F79708540.3mm5035ABT-M3BABT-M3TF7970763F79707640.3mm5035ACA-M3BACA-M3TF7970863F7970864* See page 28 for AFpak ABT-894.* See page 28 for AFpak ACA-894.

Semi-micro andmicro columnsSemi-microMicro ColumnsSemi-micro and micro columnsAFpak ACB-894 semi-micro and micro typeLigand : Cibacron blueColumns for Affinity ChromotographyAFpak ADS-894 semi-micro and micro typeLigand : Dextran sulfateBase Material : PolyhydroxymethacrylateSemi-microMicro Columns74Columns for Affinity ChromotographyI.D.2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)50355035503550355035* See page 28 for AFpak ACB-894.Product NameACB-2BACB-2TACB-1BACB-1TACB-M8BACB-M8TACB-M5BACB-M5TACB-M3BACB-M3TLigand : Ethylenediaminediacetic acidI.D.Length(mm)Product Name2.0mm50AED-2B35AED-2T1.0mm5035AED-1BAED-1T0.8mm0.5mm0.3mm505050AED-M8BAED-M5BAED-M3B353535AED-M8TAED-M5TAED-M3T* See page 28 for AFpak AED-894.Product CodeF7970923F7970924F7970933F7970934F7970943F7970944F7970953F7970954F7970963F7970964AFpak AED-894 semi-micro and micro typeLigand : GelatinProduct CodeF7971123F7971124F7971133F7971134F7971143F7971144F7971153F7971154F7971163F7971164AFpak AGE-894 semi-micro and micro typeI.D.Length(mm)Product Name2.0mm50ADS-2B35ADS-2T1.0mm5035ADS-1BADS-1T0.8mm0.5mm0.3mm505050ADS-M8BADS-M5BADS-M3B353535ADS-M8TADS-M5TADS-M3T* See page 28 for AFpak ADS-894.Ligand : N-acetylglucosamineI.D.Length(mm)Product Name2.0mm50AGA-2B35AGA-2T1.0mm5035AGA-1BAGA-1T0.8mm0.5mm0.3mm505050AGA-M8BAGA-M5BAGA-M3B353535AGA-M8TAGA-M5TAGA-M3T* See page 28 for AFpak AGA-894.Product CodeF7971023F7971024F7971033F7971034F7971043F7971044F7971053F7971054F7971063F7971064AFpak AGA-894 semi-micro and micro typeLigand : GlutathioneProduct CodeF7971223F7971224F7971233F7971234F7971243F7971244F7971253F7971254F7971263F7971264AFpak AGT-894 semi-micro and micro typeI.D.Length(mm)Product NameProduct CodeI.D.Length(mm)Product NameProduct Code2.0mm5035AGE-2BAGE-2TF7971323F79713242.0mm5035AGT-2BAGT-2TF7971423F79714241.0mm5035AGE-1BAGE-1TF7971333F79713341.0mm5035AGT-1BAGT-1TF7971433F79714340.8mm5035AGE-M8BAGE-M8TF7971343F79713440.8mm5035AGT-M8BAGT-M8TF7971443F79714440.5mm5035AGE-M5BAGE-M5TF7971353F79713540.5mm5035AGT-M5BAGT-M5TF7971453F79714540.3mm5035AGE-M3BAGE-M3TF7971363F79713640.3mm5035AGT-M3BAGT-M3TF7971463F7971464* See page 28 for AFpak AGE-894.AFpak AHR-894 semi-micro and micro typeLigand : Heparin* See page 28 for AFpak AGT-894.AFpak AIA-894 semi-micro and micro typeLigand : Iminodiacetic acidI.D.Length(mm)Product NameProduct CodeI.D.Length(mm)Product NameProduct Code2.0mm5035AHR-2BAHR-2TF7971523F79715242.0mm5035AIA-2BAIA-2TF7971623F79716241.0mm5035AHR-1BAHR-1TF7971533F79715341.0mm5035AIA-1BAIA-1TF7971633F79716340.8mm5035AHR-M8BAHR-M8TF7971543F79715440.8mm5035AIA-M8BAIA-M8TF7971643F79716440.5mm5035AHR-M5BAHR-M5TF7971553F79715540.5mm5035AIA-M5BAIA-M5TF7971653F79716540.3mm5035AHR-M3BAHR-M3TF7971563F79715640.3mm5035AIA-M3BAIA-M3TF7971663F7971664* See page 28 for AFpak AHR-894.* See page 28 for AFpak AIA-894.

AFpak ALC-894 semi-micro and micro typeLigand : Lentil lectinLengthI.D.(mm)2.0mm1.0mm0.8mm0.5mm0.3mm50355035503550355035* See page 28 for AFpak ALC-894.Product NameALC-2BALC-2TALC-1BALC-1TALC-M8BALC-M8TALC-M5BALC-M5TALC-M3BALC-M3TSemi-micro and micro columnsColumns for Affinity ChromotographyProduct CodeF7971723F7971724F7971733F7971734F7971743F7971744F7971753F7971754F7971763F7971764AFpak ANA-894 semi-micro and micro typeLigand : Nicotinamide Adenine Dinucleotide (NAD)I.D.Length(mm)Product Name Product Code2.0mm50ANA-2BF797192335ANA-2TF797192450ANA-1BF79719331.0mm35ANA-1TF79719340.8mm50ANA-M8BF797194335ANA-M8TF79719440.5mm50ANA-M5BF797195335ANA-M5TF79719540.3mm50ANA-M3BF797196335ANA-M3TF7971964AFpak ALS-894 semi-micro and micro typeLigand : LysineI.D.Length(mm)Product Name2.0mm50ALS-2B35ALS-2T1.0mm5035ALS-1BALS-1T0.8mm0.5mm0.3mm505050ALS-M8BALS-M5BALS-M3B353535ALS-M8TALS-M5TALS-M3T* See page 28 for AFpak ALS-894.Ligand : OvomucoidLengthI.D.(mm)2.0mm1.0mm0.8mm0.5mm0.3mm50355035503550355035Product NameAOV-2BAOV-2TAOV-1BAOV-1TAOV-M8BAOV-M8TAOV-M5BAOV-M5TAOV-M3BAOV-M3TSemi-microMicro ColumnsBase Material : PolyhydroxymethacrylateProduct CodeF7971823F7971824F7971833F7971834F7971843F7971844F7971853F7971854F7971863F7971864AFpak AOV-894 semi-micro and micro typeProduct CodeF7972023F7972024F7972033F7972034F7972043F7972044F7972053F7972054F7972063F7972064Semi-micro andmicro columnsSemi-microMicro Columns75Columns for Affinity Chromotography* See page 28 for AFpak ANA-894.AFpak APA-894 semi-micro and micro typeLigand : Protein A* See page 28 for AFpak AOV-894.AFpak APB-894 semi-micro and micro typeLigand : Aminophenylboronic acidI.D.Length(mm)Product NameProduct CodeI.D.Length(mm)Product NameProduct Code2.0mm5035APA-2BAPA-2TF7972123F79721242.0mm5035APB-2BAPB-2TF7972223F79722241.0mm5035APA-1BAPA-1TF7972133F79721341.0mm5035APB-1BAPB-1TF7972233F79722340.8mm5035APA-M8BAPA-M8TF7972143F79721440.8mm5035APB-M8BAPB-M8TF7972243F79722440.5mm5035APA-M5BAPA-M5TF7972153F79721540.5mm5035APB-M5BAPB-M5TF7972253F79722540.3mm5035APA-M3BAPA-M3TF7972163F79721640.3mm5035APB-M3BAPB-M3TF7972263F7972264* See page 28 for AFpak APA-894.AFpak APD-894 semi-micro and micro typeLigand : Procion red* See page 28 for AFpak APB-894.AFpak APE-894 semi-micro and micro typeLigand : PhosphorylethanolamineI.D.Length(mm)Product NameProduct CodeI.D.Length(mm)Product NameProduct Code2.0mm5035APD-2BAPD-2TF7972323F79723242.0mm5035APE-2BAPE-2TF7972423F79724241.0mm5035APD-1BAPD-1TF7972333F79723341.0mm5035APE-1BAPE-1TF7972433F79724340.8mm5035APD-M8BAPD-M8TF7972343F79723440.8mm5035APE-M8BAPE-M8TF7972443F79724440.5mm5035APD-M5BAPD-M5TF7972353F79723540.5mm5035APE-M5BAPE-M5TF7972453F79724540.3mm5035APD-M3BAPD-M3TF7972363F79723640.3mm5035APE-M3BAPE-M3TF7972463F7972464* See page 28 for AFpak APD-894.* See page 28 for AFpak APE-894.

Semi-micro andmicro columnsSemi-microMicro ColumnsLigand : Protein GSemi-micro and micro columnsAFpak APG-894 semi-micro and micro typeColumns for Affinity ChromotographyAFpak APH-894 semi-micro and micro typeLigand : PhenylalanineBase Material : PolyhydroxymethacrylateSemi-microMicro Columns76Columns for Affinity ChromotographyI.D.2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)50355035503550355035* See page 28 for AFpak APG-894.Product NameAPG-2BAPG-2TAPG-1BAPG-1TAPG-M8BAPG-M8TAPG-M5BAPG-M5TAPG-M3BAPG-M3TLigand : ProtamineI.D.Length(mm)Product Name2.0mm50APR-2B35APR-2T1.0mm5035APR-1BAPR-1T0.8mm0.5mm0.3mm505050APR-M8BAPR-M5BAPR-M3B353535APR-M8TAPR-M5TAPR-M3T* See page 28 for AFpak APR-894.Product CodeF7972523F7972524F7972533F7972534F7972543F7972544F7972553F7972554F7972563F7972564AFpak APR-894 semi-micro and micro typeProduct CodeF7972723F7972724F7972733F7972734F7972743F7972744F7972753F7972754F7972763F7972764I.D.Length(mm)Product Name2.0mm50APH-2B35APH-2T1.0mm5035APH-1BAPH-1T0.8mm0.5mm0.3mm505050APH-M8BAPH-M5BAPH-M3B353535APH-M8TAPH-M5TAPH-M3T* See page 28 for AFpak APH-894.Product CodeF7972623F7972624F7972633F7972634F7972643F7972644F7972653F7972654F7972663F7972664AFpak ARC-894 semi-micro and micro typeLigand : Ricinus communis agglutinin I (RCA-I)I.D.Length(mm)Product Name Product Code2.0mm50ARC-2BF797282335ARC-2TF79728241.0mm5035ARC-1BARC-1TF7972833F79728340.8mm0.5mm0.3mm505050ARC-M8BARC-M5BARC-M3BF7972843F7972853F7972863353535ARC-M8TARC-M5TARC-M3TF7972844F7972854F7972864* See page 28 for AFpak ARC-894.AFpak AST-894 semi-micro and micro typeLigand : Soybean trypsin inhibitorAFpak AWG-894 semi-micro and micro typeLigand : Wheat germ agglutinin (WGA)I.D.Length(mm)Product NameProduct CodeI.D.Length(mm)Product NameProduct Code2.0mm5035AST-2BAST-2TF7972923F79729242.0mm5035AWG-2BAWG-2TF7973023F79730241.0mm5035AST-1BAST-1TF7972933F79729341.0mm5035AWG-1BAWG-1TF7973033F79730340.8mm5035AST-M8BAST-M8TF7972943F79729440.8mm5035AWG-M8BAWG-M8TF7973043F79730440.5mm5035AST-M5BAST-M5TF7972953F79729540.5mm5035AWG-M5BAWG-M5TF7973053F79730540.3mm5035AST-M3BAST-M3TF7972963F79729640.3mm5035AWG-M3BAWG-M3TF7973063F7973064* See page 28 for AFpak AST-894.* See page 28 for AFpak AWG-894.

KW402.5 semi-micro and micro typeBase Material : SilicaI.D.4.6mmLength(mm)15050Product NameKW402.5-4DKW402.5-4BSemi-micro and micro columnsAqueous SEC(GFC) column : Silica-basedProduct CodeF7781212F7781213KW403 semi-micro and micro typeBase Material : SilicaLengthI.D.(mm)1504.6mm50Product NameKW403-4DKW403-4BSemi-microMicro ColumnsProduct CodeF7781312F7781313Semi-micro andmicro columns250KW402.5-2EF7781221250KW403-2EF77813212.0mm150KW402.5-2DF77812222.0mm150KW403-2DF778132250KW402.5-2BF778122350KW403-2BF7781323250KW402.5-1EF7781231250KW403-1EF7781331Semi-microMicro Columns1.0mm150KW402.5-1DF77812321.0mm150KW403-1DF7781332770.8mm0.5mm0.3mm50250150502501505025015050KW402.5-1BKW402.5-M8EKW402.5-M8DKW402.5-M8BKW402.5-M5EKW402.5-M5DKW402.5-M5BKW402.5-M3EKW402.5-M3DKW402.5-M3B* See page 44 for KW402.5-4F.* See page 85 for preparative columns.KW404 semi-micro and micro typeBase Material : SilicaI.D.4.6mmLength(mm)15050Product NameKW404-4DKW404-4BF7781233F7781241F7781242F7781243F7781251F7781252F7781253F7781261F7781262F7781263Product CodeF7781412F77814130.8mm0.5mm0.3mm50250150502501505025015050KW403-1BKW403-M8EKW403-M8DKW403-M8BKW403-M5EKW403-M5DKW403-M5BKW403-M3EKW403-M3DKW403-M3B* See page 44 for KW403-4F.* See page 85 for preparative columns.KW405 semi-micro and micro typeBase Material : SilicaI.D.4.6mmLength(mm)15050Product NameKW405-4DKW405-4BF7781333F7781341F7781342F7781343F7781351F7781352F7781353F7781361F7781362F7781363Product CodeF7781512F7781513Aqueous SEC(GFC) column : Silica-based250KW404-2EF7781421250KW405-2EF77815212.0mm150KW404-2DF77814222.0mm150KW405-2DF778152250KW404-2BF778142350KW405-2BF7781523250KW404-1EF7781431250KW405-1EF77815311.0mm150KW404-1DF77814321.0mm150KW405-1DF778153250KW404-1BF778143350KW405-1BF7781533250KW404-M8EF7781441250KW405-M8EF77815410.8mm150KW404-M8DF77814420.8mm150KW405-M8DF778154250KW404-M8BF778144350KW405-M8BF7781543250KW404-M5EF7781451250KW405-M5EF77815510.5mm150KW404-M5DF77814520.5mm150KW405-M5DF778155250KW404-M5BF778145350KW405-M5BF7781553250KW404-M3EF7781461250KW405-M3EF77815610.3mm150KW404-M3DF77814620.3mm150KW405-M3DF778156250KW404-M3BF778146350KW405-M3BF7781563* See page 44 for KW404-4F.* See page 85 for preparative columns.* See page 44 for KW405-4F.* See page 85 for preparative columns.

Semi-micro andmicro columnsSemi-microMicro Columns78Aqueous SEC(GFC) columns : Polymer-basedSemi-microMicro ColumnsI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmSemi-micro and micro columnsLength(mm)250150502501505025015050250150502501505025015050Aqueous SEC(GFC) columns : Polymer-basedOHpak SB-802 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateProduct NameSB802-4ESB802-4DSB802-4BSB802-2ESB802-2DSB802-2BSB802-1ESB802-1DSB802-1BSB802-M8ESB802-M8DSB802-M8BSB802-M5ESB802-M5DSB802-M5BSB802-M3ESB802-M3DSB802-M3B* See page 46 for OHpak SB-802 HQ.* See page 85 for preparative columns.I.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameSB803-4ESB803-4DSB803-4BSB803-2ESB803-2DSB803-2BSB803-1ESB803-1DSB803-1BSB803-M8ESB803-M8DSB803-M8BSB803-M5ESB803-M5DSB803-M5BSB803-M3ESB803-M3DSB803-M3BProduct CodeF7770111F7770112F7770113F7770121F7770122F7770123F7770131F7770132F7770133F7770141F7770142F7770143F7770151F7770152F7770153F7770161F7770162F7770163OHpak SB-803 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateProduct CodeF7770311F7770312F7770313F7770321F7770322F7770323F7770331F7770332F7770333F7770341F7770342F7770343F7770351F7770352F7770353F7770361F7770362F7770363OHpak SB-802.5 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameSB802.5-4ESB802.5-4DSB802.5-4BSB802.5-2ESB802.5-2DSB802.5-2BSB802.5-1ESB802.5-1DSB802.5-1BSB802.5-M8ESB802.5-M8DSB802.5-M8BSB802.5-M5ESB802.5-M5DSB802.5-M5BSB802.5-M3ESB802.5-M3DSB802.5-M3B* See page 46 for OHpak SB-802.5 HQ.* See page 85 for preparative columns.I.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameSB804-4ESB804-4DSB804-4BSB804-2ESB804-2DSB804-2BSB804-1ESB804-1DSB804-1BSB804-M8ESB804-M8DSB804-M8BSB804-M5ESB804-M5DSB804-M5BSB804-M3ESB804-M3DSB804-M3BProduct CodeF7770211F7770212F7770213F7770221F7770222F7770223F7770231F7770232F7770233F7770241F7770242F7770243F7770251F7770252F7770253F7770261F7770262F7770263OHpak SB-804 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateProduct CodeF7770411F7770412F7770413F7770421F7770422F7770423F7770431F7770432F7770433F7770441F7770442F7770443F7770451F7770452F7770453F7770461F7770462F7770463* See page 46 for OHpak SB-803 HQ.* See page 85 for preparative columns.* See page 46 for OHpak SB-804 HQ.* See page 85 for preparative columns.

I.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Semi-micro and micro columnsAqueous SEC(GFC) columns : Polymer-basedOHpak SB-805 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateProduct NameSB805-4ESB805-4DSB805-4BSB805-2ESB805-2DSB805-2BSB805-1ESB805-1DSB805-1BSB805-M8ESB805-M8DSB805-M8BSB805-M5ESB805-M5DSB805-M5BSB805-M3ESB805-M3DSB805-M3B* See page 46 for OHpak SB-805 HQ.* See page 85 for preparative columns.Product CodeF7770511F7770512F7770513F7770521F7770522F7770523F7770531F7770532F7770533F7770541F7770542F7770543F7770551F7770552F7770553F7770561F7770562F7770563OHpak SB-806 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameSB806-4ESB806-4DSB806-4BSB806-2ESB806-2DSB806-2BSB806-1ESB806-1DSB806-1BSB806-M8ESB806-M8DSB806-M8BSB806-M5ESB806-M5DSB806-M5BSB806-M3ESB806-M3DSB806-M3B* See page 46 for OHpak SB-806 HQ.* See page 85 for preparative columns.Semi-microMicro ColumnsProduct CodeF7770611F7770612F7770613F7770621F7770622F7770623F7770631F7770632F7770633F7770641F7770642F7770643F7770651F7770652F7770653F7770661F7770662F7770663Semi-micro andmicro columnsSemi-microMicro Columns79Aqueous SEC(GFC) columns : Polymer-based

Semi-micro andmicro columnsSemi-microMicro Columns80Multimode ColumnsSemi-microMicro ColumnsSemi-micro and micro columnsAsahipak GS-220 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameGS220A-4EGS220A-4DGS220A-4BGS220A-2EGS220A-2DGS220A-2BGS220A-1EGS220A-1DGS220A-1BGS220A-M8EGS220A-M8DGS220A-M8BGS220A-M5EGS220A-M5DGS220A-M5BGS220A-M3EGS220A-M3DGS220A-M3BMultimode ColumnsProduct CodeF7750211F7750212F7750213F7750221F7750222F7750223F7750231F7750232F7750233F7750241F7750242F7750243F7750251F7750252F7750253F7750261F7750262F7750263Asahipak GS-320 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameGS320A-4EGS320A-4DGS320A-4BGS320A-2EGS320A-2DGS320A-2BGS320A-1EGS320A-1DGS320A-1BGS320A-M8EGS320A-M8DGS320A-M8BGS320A-M5EGS320A-M5DGS320A-M5BGS320A-M3EGS320A-M3DGS320A-M3BProduct CodeF7750311F7750312F7750313F7750321F7750322F7750323F7750331F7750332F7750333F7750341F7750342F7750343F7750351F7750352F7750353F7750361F7750362F7750363* See page 48 for Asahipak GS-220 HQ.* See page 86 for preparative columns.* See page 48 for Asahipak GS-320 HQ.* See page 86 for preparative columns.Asahipak GS-520 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameGS520A-4EGS520A-4DGS520A-4BGS520A-2EGS520A-2DGS520A-2BGS520A-1EGS520A-1DGS520A-1BGS520A-M8EGS520A-M8DGS520A-M8BGS520A-M5EGS520A-M5DGS520A-M5BGS520A-M3EGS520A-M3DGS520A-M3B* See page 48 for Asahipak GS-520 HQ.* See page 86 for preparative columns.Product CodeF7750511F7750512F7750513F7750521F7750522F7750523F7750531F7750532F7750533F7750541F7750542F7750543F7750551F7750552F7750553F7750561F7750562F7750563Asahipak GS-620 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameGS620A-4EGS620A-4DGS620A-4BGS620A-2EGS620A-2DGS620A-2BGS620A-1EGS620A-1DGS620A-1BGS620A-M8EGS620A-M8DGS620A-M8BGS620A-M5EGS620A-M5DGS620A-M5BGS620A-M3EGS620A-M3DGS620A-M3B* See page 48 for Asahipak GS-620 HQ.* See page 86 for preparative columns.Product CodeF7750611F7750612F7750613F7750621F7750622F7750623F7750631F7750632F7750633F7750641F7750642F7750643F7750651F7750652F7750653F7750661F7750662F7750663

Asahipak GF-310 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameGF310A-4EGF310A-4DGF310A-4BGF310A-2EGF310A-2DGF310A-2BGF310A-1EGF310A-1DGF310A-1BGF310A-M8EGF310A-M8DGF310A-M8BGF310A-M5EGF310A-M5DGF310A-M5BGF310A-M3EGF310A-M3DGF310A-M3BSemi-micro and micro columnsAqueous/Organic SEC ColumnsProduct CodeF7760311F7760312F7760313F7760321F7760322F7760323F7760331F7760332F7760333F7760341F7760342F7760343F7760351F7760352F7760353F7760361F7760362F7760363Asahipak GF-510 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameGF510A-4EGF510A-4DGF510A-4BGF510A-2EGF510A-2DGF510A-2BGF510A-1EGF510A-1DGF510A-1BGF510A-M8EGF510A-M8DGF510A-M8BGF510A-M5EGF510A-M5DGF510A-M5BGF510A-M3EGF510A-M3DGF510A-M3BSemi-microMicro ColumnsProduct CodeF7760511F7760512F7760513F7760521F7760522F7760523F7760531F7760532F7760533F7760541F7760542F7760543F7760551F7760552F7760553F7760561F7760562F7760563Semi-micro andmicro columnsSemi-microMicro Columns81Aqueous/Organic SEC Columns* See page 50 for Asahipak GF-310 HQ.* See page 86 for preparative columns.* See page 50 for Asahipak GF-510 HQ.* See page 86 for preparative columns.Asahipak GF-710 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmLength(mm)250150502501505025015050250150502501505025015050Product NameGF710A-4EGF710A-4DGF710A-4BGF710A-2EGF710A-2DGF710A-2BGF710A-1EGF710A-1DGF710A-1BGF710A-M8EGF710A-M8DGF710A-M8BGF710A-M5EGF710A-M5DGF710A-M5BGF710A-M3EGF710A-M3DGF710A-M3B* See page 50 for Asahipak GF-710 HQ.* See page 86 for preparative columns.Product CodeF7760711F7760712F7760713F7760721F7760722F7760723F7760731F7760732F7760733F7760741F7760742F7760743F7760751F7760752F7760753F7760761F7760762F7760763

Preparative columnsPreparativeColumnsPreparative columns (I.D. 10mm~50mm)Polymer-based Packed Columns for Reversed Phase ChromatographyAsahipak ODP-50 Preparative typeProduct CodeF6820001Product NameAsahipak ODP-50 10EPlate Number(TP/column)≥ 10,000FunctionalGroupOctadecylParticle Size(μm)5Column Size (mm)I.D. x L10.0 x 250F6820035Asahipak ODP-90 20F≥ 9,000Octadecyl920.0 x 300F6820019Asahipak ODP-130 28F≥ 9,000Octadecyl1328.0 x 300F6710004Asahipak ODP-130G 7B(guard column)Octadecyl137.5 x 50F6714029Asahipak ODP-130G 20C(guard column)Octadecyl1320.0 x 100PreparativeColumns82* See page 10 for Asahipak ODP-50.* See page 70 for semi-micro columns and micro columns.Base Material : Polyvinyl alcoholPolymer-based Packed Columns for Reversed Phase ChromatographyAsahipak C8P-50 Preparative typeProduct CodeProduct NameF6820003 Asahipak C8P-50 10EF6714004 Asahipak C8P-50G 7B* See page 10 for Asahipak C8P-50.Asahipak C4P-50 Preparative typeProduct CodeProduct NameF6820005 Asahipak C4P-50 10EF6714005 Asahipak C4P-50G 7B* See page 10 for Asahipak C4P-50.RSpak DE-613 Preparative typeProduct CodeProduct NameF6513013 RSpak DE-2013F6700190 RSpak DE-LGF6513015 RSpak DE-5013F6700191 RSpak DE-LLG* See page 11 for RSpak DE-613.* See page 70 for semi-micro columns and micro columns.RSpak DM-614 Preparative typePlate Number(TP/column)≥ 8,000(guard column)Plate Number(TP/column)≥ 7,000(guard column)Plate Number(TP/column)≥ 10,000(guard column)−(guard column)FunctionalGroupOctylOctylFunctionalGroupButylButylFunctionalGroup−−−−Particle Size(μm)55Particle Size(μm)55Particle Size(μm)12121212Column Size (mm)I.D. x L10.0 × 2507.5 × 50Base Material : Polyvinyl alcoholColumn Size (mm)I.D. x L10.0 × 2507.5 × 50Base Material : Polyvinyl alcoholColumn Size (mm)I.D. x L20.0 × 3008.0 × 5050.0 × 30020.0 × 100Base Material : PolymethacrylateProduct CodeProduct NamePlate Number(TP/column)FunctionalGroupParticle Size(μm)Column Size (mm)I.D. x LF6514014RSpak DM-2014≥ 5,000−1220.0 × 300F6700404RSpak DM-LG(guard column)−128.0 × 50F6514022RSpak DM-5014−−1250.0 × 300F6700162RSpak DM-LLG(guard column)−1220.0 × 100* See page 11 for RSpak DM-614.Base Material : Polyhydroxymethacrylate*Contact Shodex or our distributors near you for larger preparative columns.

Preparative columnsPolymer-based Packed Columns for Hydrophilic Interaction Chromatography (HILIC)Asahipak NH2P Preparative typeProduct CodeF6830001Product NameAsahipak NH2P-50 10EPlate Number(TP/column)≥ 10,000FunctionalGroupAminoParticle Size(μm)5PreparativeColumnsColumn Size (mm)I.D. x L10.0 × 250Preparative columnsF6830031Asahipak NH2P-90 20F≥ 9,000Amino920.0 × 300F6830007Asahipak NH2P-130 28F≥ 1,000Amino1328.0 × 300F6710017Asahipak NH2P-130G 7B(guard column)Amino137.5 × 50* See page 13 for Asahipak NH2P-50.* See page 70 for semi-micro columns and micro columns.Base Material : Polyvinyl alcoholPreparativeColumns83Silica-based Packed Columns for Reversed Phase ChromatographySilica C18M Preparative typeProduct CodeProduct NameF7560040 Silica C18M 10EF7560041 Silica C18M 20E* See page 20 for Silica C18M.Silica 5C8 Preparative typeProduct CodeProduct NameF7560062 Silica 5C8 10EF7560063 Silica 5C8 20E* See page 20 for Silica 5C8.Silica 5C4 Preparative typeProduct CodeProduct NameF7560054 Silica 5C4 10EF7560055 Silica 5C4 20E* See page 20 for Silica 5C4.Particle Size(μm)55Silica-based Packed Columns for Normal Phase and Hydrophilic Interaction Chromatography (HILIC)F7560050 Silica 5SIL 10EF7560051 Silica 5SIL 20E* See page 20 for Silica 5SILF7560060 Silica 5NH 10EF7560061 Silica 5NH 20E* See page 20 for Silica 5NH.Plate Number(TP/column)≥ 16,000≥ 16,000Plate Number(TP/column)≥ 15,000≥ 15,000Plate Number(TP/column)≥ 15,000≥ 15,000≥ 15,000≥ 15,000Plate Number(TP/column)≥ 8,000≥ 8,000FunctionalGroupOctadecylOctadecylFunctionalGroupOctylOctylFunctionalGroupButylButylSilica 5SIL Preparative type [Columns for Normal Phase Chromatography]Product CodeProduct CodeProduct NameProduct NamePlate Number(TP/column)FunctionalGroup−−FunctionalGroupAminopropylAminopropylParticle Size(μm)55Particle Size(μm)55Particle Size(μm)55Particle Size(μm)55Column Size (mm)I.D. x L10.0 × 25020.0 × 250Base Material : SilicaColumn Size (mm)I.D. x L10.0 × 25020.0 × 250Base Material : SilicaColumn Size (mm)I.D. x L10.0 × 25020.0 × 250Base Material : SilicaColumn Size (mm)I.D. x L10.0 × 25020.0 × 250Base Material : SilicaSilica 5NH Preparative type [Columns for Normal Phase and Hydrophilic Interaction Chromatography (HILIC)]Column Size (mm)I.D. x L10.0 × 25020.0 × 250Base Material : Silica*Contact Shodex or our distributors near you for larger preparative columns.Polymer-based Packed Columns for Hydrophilic Interaction Chromatography (HILIC)Silica-based Packed Columns for Reversed Phase, Normal Phase and Hydrophilic Interaction Chromatography (HILIC)

Preparative columnsPreparativeColumns84Columns for Anion Exchange Chromatography Columns for Cation Exchange ChromatographyPreparativeColumnsF6548000F6548020F6548050F6709602F6548001F6548021F6598051F6709603F6840004F6710021F6548002F6548022F6548052F6709604F6548003F6548023F6548053F6709605Preparative columnsIEC QA-825 Preparative typeProduct CodeProduct NameIEC QA-2025IEC QA-2825IEC QA-5025IEC QA-LGIEC DEAE-825 Preparative typeProduct CodeProduct NameIEC DEAE-2025IEC DEAE-2825IEC DEAE-5025IEC DEAE-LGAsahipak ES-502N Preparative typeProduct CodeProduct NameAsahipak ES-502N 20CAsahipak GS-20G 7BIEC SP-825 Preparative typeProduct CodeIEC SP-2025IEC SP-2825IEC SP-5025IEC SP-LGIEC CM-2025IEC CM-2825IEC CM-5025IEC CM-LGColumns for Anion Exchange ChromatographyFunctional GroupQuaternary ammoniumQuaternary ammoniumQuaternary ammonium(guard column)DiethylaminoethylDiethylaminoethylDiethylaminoethyl(guard column)Diethylaminoethyl(guard column)Particle Size(μm)20373720Particle Size(μm)20373720Column Size (mm)I.D. x L20.0 × 15028.0 × 30050.0 × 3008.0 × 50Column Size (mm)I.D. x L20.0 × 15028.0 × 30050.0 × 3008.0 × 50Columns for Cation Exchange ChromatographyProduct NameIEC CM-825 Preparative typeProduct CodeProduct NameFunctional GroupFunctional GroupFunctional GroupSulfopropylSulfopropylSulfopropyl(guard column)Functional GroupCarboxymethylCarboxymethylCarboxymethyl(guard column)Base Material : PolyhydroxymethacrylateBase Material : PolyhydroxymethacrylateParticle Size(μm)1320Base Material : Polyvinyl alcoholParticle Size(μm)2020.0 × 1503728.0 × 3003750.0 × 300208.0 × 50Base Material : PolyhydroxymethacrylateParticle Size(μm)20373720Column Size (mm)I.D. x L20.0 × 1007.5 × 50Column Size (mm)I.D. x LColumn Size (mm)I.D. x L20.0 × 15028.0 × 30050.0 × 3008.0 × 50Base Material : Polyhydroxymethacrylate* See page 24 for IEC QA-825.* See page 71 for semi-microcolumns and micro columns.* See page 24 for IEC DEAE-825.* See page 71 for semi-microcolumns and micro columns.* See page 24 for Asahipak ES-502N.* See page 71 for semi-microcolumns and micro columns.* See page 26 for IEC SP-825.* See page 72 for semi-microcolumns and micro columns.* See page 26 for IEC CM-825.* See page 72 for semi-microcolumns and micro columns.Asahipak ES-502C Preparative typeProduct Code Product NameFunctional GroupF6840003 Asahipak ES-502C 20C CarboxymethylF6710021 Asahipak GS-20G 7B (guard column)Particle Size(μm)1320Column Size (mm)I.D. x L20.0 × 1007.5 × 50Base Material : Polyvinyl alcohol* See page 26 for Asahipak ES-502C.* See page 72 for semi-microcolumns and micro columns.*Contact Shodex or our distributors near you for larger preparative columns.

Columns for Ligand Exchange ChromatographySUGAR KS-800 series Preparative typeProduct Code Product NamePlate Number(TP/column)Counter IonParticle Size(μm)Column Size (mm)I.D. x LStandard ColumnF6502007F6502008F6502009F6502010F6502011F6502012F6700002SUGAR KS-2001SUGAR KS-2002SUGAR KS-2003SUGAR KS-2004SUGAR KS-2005SUGAR KS-2006SUGAR KS-LG≥ 7,000≥ 7,000≥ 8,000≥ 6,000≥ 6,000≥ 6,000(guard column)Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )1313131818181320.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 3008.0 × 50KS-801KS-802KS-803KS-804KS-805KS-806(guard column)* See page 32 for SUGAR KS-800.Base Material : Styrene divinylbenzene copolymerRSpak DC-613 Preparative typeProduct CodeF6514013 RSpak DC-2013F6700402 RSpak DC-LGF6514021 RSpak DC-5013F6700172 RSpak DC-LLG* See page 33 for RSpak DC-613.F6505012 RSpak KC-2011F6700010 RSpak KC-LG* See page 36 for RSpak KC-811.Plate Number(TP/column)≥ 6,000(guard column)−(guard column)Columns for Ion-exclusion ChromatographyPlate Number(TP/column)≥ 8,000(guard column)PROTEIN KW-800 series Preparative typeProduct Code Product NamePlate Number(TP/column)F6505020F6505021F6505022F6709556PROTEIN KW-2002.5PROTEIN KW-2003PROTEIN KW-2004PROTEIN KW-LG≥ 17,000≥ 17,000≥ 14,000(guard column)* See page 44 for PROTEIN KW-800.* See page 77 for semi-micro columns and micro columns.F6516011F6516012F6516013F6516014F6516015F6516016F6516017F6709555Product NameRSpak KC-811 Preparative typeProduct CodeProduct NameOHpak SB-2002OHpak SB-2002.5OHpak SB-2003OHpak SB-2004OHpak SB-2005OHpak SB-2006OHpak SB-2006MOHpak SB-LG* See page 46 for OHpak SB-800 HQ.* See page 78, 79 for semi-micro columns and micro columns.Aqueous SEC(GFC) columns : Silica-basedAqueous SEC(GFC) columns : Polymer-basedOHpak SB-800 HQ series Preparative typeProduct CodeProduct NamePlate Number(TP/column)≥ 9,000≥ 12,000≥ 12,000≥ 12,000≥ 12,000≥ 12,000≥ 12,000(guard column)Counter IonSulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )1010101020.0 × 3008.0 × 5050.0 × 30020.0 × 100Base Material : Styrene divinylbenzene copolymerFunctional GroupSulfoSulfoPreparative columnsParticle Size(μm)Particle Size(μm)1313Particle Size(μm)5577Particle Size(μm)1510101820201318Column Size (mm)I.D. x LColumn Size (mm)I.D. x L20.0 × 3008.0 × 50Base Material : Styrene divinylbenzene copolymerColumn Size (mm)I.D. x L20.0 × 30020.0 × 30020.0 × 3008.0 × 50Column Size (mm)I.D. x L20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 3008.0 × 50PreparativeColumnsStandard ColumnKW-802.5KW-803KW-804(guard column)Base Material : SilicaStandard ColumnSB-802 HQSB-802.5 HQSB-803 HQSB-804 HQSB-805 HQSB-806 HQSB-806M HQ(guard column)Base Material : PolyhydroxymethacrylatePreparative columnsPreparativeColumns85Columns for Ligand Exchange Chromatography Columns for Ion-exclusion ChromatographyAqueous SEC(GFC) columns : Silica-based Aqueous SEC(GFC) columns : Polymer-based(Note) The maximum solvent tolerance of the SB-2000 series for methanol, acetonitrile, and DMF is 50%.Solvent tolerance differs from that of the standard SB-800 HQ columns.*Contact Shodex or our distributors near you for larger preparative columns.

Preparative columnsPreparativeColumns86Multimode columns Aqueous/Organic SEC columns Organic SEC (GPC) columnsPreparativeColumnsProduct CodeProduct CodeF6810030F6810031F6810032F6810033F6810038F6810039F6810040F6810041F6710020Asahipak GS-310 20FAsahipak GS-510 20FAsahipak GS-710 20FAsahipak GSM-700 20FAsahipak GS-310 20GAsahipak GS-510 20GAsahipak GS-710 20GAsahipak GSM-700 20GAsahipak GS-10G 7BMultimode columnsAqueous/Organic SEC columnsParticle Size(μm)131313131313131320Organic SEC (GPC) columnsColumn Size (mm)I.D. x L20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 50020.0 × 50020.0 × 50020.0 × 5007.5 × 50GF-310 HQGF-510 HQGF-710 HQGF-7M HQGF-310 HQGF-510 HQGF-710 HQGF-7M HQ(guard column)* See page 50 for Asahipak GF-HQ.Base Material : Polyvinyl alcohol* See page 81 for semi-micro columns and micro columns.(Note) The solvents, usable for GS-710 20F and 20G are water and methanol.Solvent tolerance differs from the standard GF-710 HQ column.Selection of GSM-700 20F or 20G is recommended in case of use of other solvents for scale-up testing with GF-710 HQ.Product CodeF6102401F6102402F6102425F6102403F6102404F6102405F6102406F6102409F6700406Preparative columnsAsahipak GS-HQ series Preparative typeF6810017F6810018F6810019F6810020F6810034F6810035F6810036F6810037F6710021Product NameAsahipak GS-220 20FAsahipak GS-320 20FAsahipak GS-520 20FAsahipak GS-620 20FAsahipak GS-220 20GAsahipak GS-320 20GAsahipak GS-520 20GAsahipak GS-620 20GAsahipak GS-20G 7B* See page 48 for Asahipak GS-HQ.* See page 80 for semi-micro columns and micro columns.Asahipak GF-HQ series Preparative typeProduct NameGPC KF-800 series Preparative typeProduct NameGPC KF-2001GPC KF-2002GPC KF-2002.5GPC KF-2003GPC KF-2004GPC KF-2005GPC KF-2006GPC KF-2006MGPC KF-LGPlate Number(TP/column)≥ 8,000≥ 8,000≥ 8,000≥ 8,000≥ 14,000≥ 14,000≥ 14,000≥ 14,000(guard column)Plate Number(TP/column)≥ 8,000≥ 8,000≥ 8,000≥ 8,000≥ 14,000≥ 14,000≥ 14,000≥ 14,000(guard column)Plate Number(TP/column)≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 14,000≥ 10,000≥ 10,000≥ 10,000(guard column)Particle Size(μm)131313131313131320Particle Size(μm)6666710101015Column Size (mm)I.D. x L20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 50020.0 × 50020.0 × 50020.0 × 5007.5 × 50Column Size (mm)I.D. x L20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 3008.0 × 50Standard ColumnGS-220 HQGS-320 HQGS-520 HQGS-620 HQGS-220 HQGS-320 HQGS-520 HQGS-620 HQ(guard column)Base Material : Polyvinyl alcoholStandard ColumnStandard ColumnKF-801KF-802KF-802.5KF-803KF-804KF-805KF-806KF-806M(guard column)* See page 52 for GPC KF-800.Base Material : Styrene divinylbenzene copolymer*Contact Shodex or our distributors near you for larger preparative columns.

GPC K-800 series Preparative typeProduct CodeF6102301F6102312F6102315F6102303F6102304F6102305F6102306F6102309F6700407Product CodeF6102001F6102002F6102025F6102003F6102004F6102005F6102006F6102009F6700310Product NameGPC K-2001GPC K-2002GPC K-2002.5GPC K-2003GPC K-2004GPC K-2005GPC K-2006GPC K-2006MGPC K-LGProduct NameGPC H-2001GPC H-2002GPC H-2002.5GPC H-2003GPC H-2004GPC H-2005GPC H-2006GPC H-2006MGPC H-GOrganic SEC (GPC) columnsPlate Number(TP/column)≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 14,000≥ 10,000≥ 10,000≥ 10,000(guard column)Organic SEC (GPC) columnsPlate Number(TP/column)≥ 13,000≥ 13,000≥ 13,000≥ 13,000≥ 13,000≥ 13,000≥ 13,000≥ 12,000(guard column)Preparative columnsParticle Size(μm)6666710101015Particle Size(μm)151515151515151515Column Size (mm)I.D. x L20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 3008.0 × 50Column Size (mm)I.D. x L20.0 × 50020.0 × 50020.0 × 50020.0 × 50020.0 × 50020.0 × 50020.0 × 50020.0 × 5008.0 × 50PreparativeColumnsStandard ColumnK-801K-802K-802.5K-803K-804K-805K-806K-806M(guard column)* See page 52 for GPC K-800. Base Material : Styrene divinylbenzene copolymerCustom-made Preparative ColumnsGPC K-800 series Preparative typeStandard ColumnK-801K-802K-802.5K-803K-804K-805K-806K-806M(guard column)Preparative columnsPreparativeColumns87Organic SEC (GPC) columns Custom-made Preparative Columns* See page 52 for GPC K-800.Base Material : Styrene divinylbenzene copolymerGPC KF-800 series Preparative typeProduct CodeF6108010Product NameGPC KF-5001Particle Size(μm)15Column Size (mm)I.D. x L50.0 × 300Standard ColumnKF-801F6108020GPC KF-50021550.0 × 300KF-802F6108025GPC KF-5002.51550.0 × 300KF-802.5F6108030GPC KF-50031550.0 × 300KF-803F6108040GPC KF-50041550.0 × 300KF-804F6700408GPC KF-LLG1520.0 × 100(guard column)* See page 52 for GPC KF-800.Base Material : Styrene divinylbenzene copolymer*Contact Shodex or our distributors near you for larger preparative columns.

Information Column Cleaning Procedures88Column Cleaning ProceduresChange in peak shapes, elution timing, and the elevated column pressure may be resolved by cleaningthe column. This section describes general indications of column deterioration and column cleaningprocedures. The details of column cleaning procedures should be referred to each column’s operationmanual.Typical indicators of column deterioration1. Elevated column pressure2. Abnormal peak shapes (broad, leading, or tailing) and split peaks3. Change in retention time4. Unstable baselineSelection guide to the cleaning solventChoose a solvent capable of dissolving the adsorbed substances.Choose the strong eluent for the choice of column.*Use the solvent specified in the operation manualStandard cleaning proceduresFor an efficient cleaning, reverse the direction and reduce the flow to 1/3 of the regular flow rate.Reversed phase columnsIon exchange chromatographyClean the columns with solvent containing higher concentration of organic solvent such asmethanol, acetonitrile, or THF.(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organicsolvents need to be checked)• Contamination caused by ionic adsorptionUse solvent with higher salt concentration or solvent with different pH from the mobilephase.• Contamination caused by hydrophobic adsorptionUse solvent containing small percentage of organic solvent.(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organicsolvents need to be checked)• Contamination caused by proteinsInject 1-2 mL of 0.1 M NaOH (aq) or 30% (v/v) acetic acid several times.Sugar analysis columnsHydrophobic interaction columnsAqueous SEC(GFC) columns[Ligand exchange columns (SUGAR series)]• In case of counter-ion detachmentFlush or inject solvent containing the salt corresponding to the modified counter-ligand.[Polymer-base amino columns (NH2P series)]• To neutralize the acidic form of amino function groupFlush with solvents in the following sequence: water, 0.1M perchloric acid, water, 0.1MNaOH (aq), water, and mobile phase.• Contamination caused by proteinsInject 1-2 mL of 0.1 M NaOH (aq) or 30% (v/v) acetic acid several times.• Contamination caused by ionic adsorptionUse solvent with higher salt concentration or solvent with different pH from the mobilephase.• Contamination caused by hydrophobic adsorptionUse solvent containing small percentage of organic solvent.(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organicsolvents need to be checked)*The volume of the cleaning solvent required is 5-10 times the column volume.*Avoid pressure elevation during the cleaning*The cleaning is limited and does not guarantee the full regeneration of the column to its original condition.For your informationOne typical cause of the column pressure elevation is the clogging of solid substances at the inlet filter of the column.In this case, reverse the direction and reduce the flow to 1/3 of the regular flow rate. This may remove the solidsubstance causing the elevated pressure.*Use the solvent specified in the operation manual

General precautions for column handlingFor the best performance of the column, please follow the instructions given below.Column mounting• Before mounting the column, replace the eluent within all the HPLC system with the mobile phase used for theanalysis. *If the mobile phase of the choice is not miscible with the eluent already in the system, use solvent that ismiscible with both solvents first to clean the system. *Buffer or salt solution may precipitate when mixed withorganic solvent of different concentrations.• Attach the column in the direction as indicated by arrow marked on the column. Gradually increase the flow rate ofthe solvent introduced to the column.• When heating the column, use low flow rate until the temperature reaches the desired level, and then graduallyincrease the flow rate up to the requirement.Column dismounting• When column was heated, turn off the heater while keeping the flow rate at 1/3 of the regular flow.• Turn off the pump when the column is cooled to room temperature.Column storage• For long-term storage, replace the solvent with shipping solution and keep the end caps tight.• Store the column in a location with stable temperature.• For the long-term storage of SEC columns, immersion method is recommended.*Please refer to the immersion method on the operation manual.Information General precautions for column handling89Other• Avoid physical shock on the column. Be cautious not to drop the column from a high position.• Do not bend the column.• Avoid opening the column’s end-fitting, it can cause alteration of column’s performance.* Read the operating manual before using the column.

InstrumentsRefractive Index DetectorShodex RI−201The RI-201 is a highly sensitive RI detector incorporatinga three-chamber flow cell.FeaturesA novel optical system (three-chamber flow cell) provides atleast twice the sensitivity of our previous detectors.The double temperature control method significantly reducesdrift caused by room temperature fluctuations.The limit of detection for saccharides is approximately 2ng.90Refractive Index DetectorRefractive Index DetectorShodex RI−201HFeaturesUses the same optical system as that of RI-101.Reasonable priceProduct CodeModelFlow cell typeMeasurement methodRefractive Index rangeMeasurement rangeDrift *Linearity rangeNoise **ResponseAuto zeroAuto zero rangeOff-set rangeOff-set resolutionIntegrator output(Sensitivity)Cell volume(Usual)Flow rate(Max.)Maximum back pressureInternal volumeRecorder outputExternal inputExternal OutputTemperature controlCommunication portOperator support functionWetted materialsPower source, Power consumptionDimension, WeightAccessories*Pure water 1mL/min, PURGE OFF**Pure water, response : 1.5secF4010105F4010106RI-201RI-201HAnalysis3 chamber-type 2 chamber-typeDeflection type1.00~1.750.125~256RIU 0.25~512RIU0.1μRIU/h0.2μRIU/h≥ 300μRIU≥ 600μRIU≤ 1nRIU≤ 2.5nRIU0.1, 0.25, 0.5, 1, 1.5, 2, 3, 6secFull auto zeroAll range5μRIU10μRIU25nRIU50nRIUDC 0~1V(4mV/μRIU, 16mV/μRIU)DC 0~1V(2mV/μRIU, 8mV/μRIU)8μL0.2~3.0mL/min10mL/min (solvent ; pure water)50kPaIN Cell ; 80μL IN Cell ; 60μLCell OUT ; 600μL Cell OUT ; 600μLAll (Cell OUT) ; 690μL All (Cell OUT) ; 670μL0~10mV/FS—READY (temperature control)LEAKERROR (ROM, RAM, PARAMETER, HOME-POSITION,OVER-HEAT, OPT.-BALANCE, INTENSITY)OFF, 30~55˚C (1˚C step),77˚C Temp. fuse (Double Temperature control)USBNoneStainless steel 316, Teflon, Quartz GlassAC100~240V±10%, 50/60Hz, 150VA maxW260 x D400 x H150 (mm), ca. 12kgPower cable, signal cable, connector tube,fuse, operation manualPrinciple of new optical systemmeasurementIn our previous optical system, themeasurement light passing through theflow cell was refracted only once. The newthree-chamber flow cell allows the light tobe refracted twice, thereby increasingsensitivity at least two-times at the sameoptical path length. This doubles thedefection degree and results in not onlyreduces the noise half, but reduces thedrift caused by optical systems half.3 chamber-typeflow cellRI-201mV1.51.41.31.21.11.0mV0.40.30.20.10.0-0.1θApplicationsample cell2θreference cellSucrose (20ng)Sucrose (20ng)Current 2 chamber-typeflow cellRI-100 series, RI-201H3 chamber-type flow cellFructose (20ng)Current 2 chamber-type flow cellFructose (20ng)θ

Refractive Index DetectorShodex RI−101, 102, 104The Shodex RI-100 series is a versatile and highsensitive RI detectors that can be used with variousmanufactures’ HPLC systems. It consists of a color-LCD,an automatic start-up function, and a validation wizard.FeaturesEquipped with a color liquid crystal display, the detectormonitors analytical status in real time.The automatic start-up function controls the complicatedoperations such as blank substitution and baselinestabilization automatically.The validation wizard enables easy component validation.Improved temparature control system shortens the requiredwarm-up time and provides stable background.The leak sensor automatically stops the pump in case ofsolvent leakage.External input and output terminals and RS232Ccommunication ports can be used to connect various HPLCsystems for an advanced automation.Product CodeModelFlow cell typeMeasuring methodRefractive Index rangeMeasurement rangeDrift *Linearity rangeNoise **ResponseAuto zeroAuto zero rangeOff-set rangeOff-set resolutionIntegrator output(Sensitivity)Cell volume(Usual)Flow rate(Max.)Maximum back pressureInternal volumeF4010101F4010104RI-101RI-104AnalysisSemi-micro2 chamber-typeDeflection type1.00~1.750.25~512μRIU0.25~512μRIU0.2μRIU/h0.2μRIU/h≥ 600μRIU≥ 600μRIU≤ 2.5nRIU≤ 5nRIU0.1, 0.25, 0.5, 1, 1.5, 2, 3, 6secFull auto zeroAll range0~500mV (Same as integrator output)10mV (Same as integrator output)DC 0~1V(2mV/μRIU, 8mV/μRIU)8μL0.2~3.0mL/min10mL/min (solvent ; pure water)50kPaIN Cell : ca. 60μLCell OUT : ca. 600μLAll (Cell OUT) : ca. 670μLDC 0~1V(2mV/μRIU, 8mV/μRIU)2.5μL0.2~1.0mL/min1.0mL/min (solvent ; pure water)IN Cell : ca. 10μLCell OUT : ca. 355μLAll (Cell OUT) : ca. 370μLF4010102RI-102Preparative2.5~5120μRIU2μRIU/h≥ 6000μRIU≤ 25nRIUDC 0~1V(0.2mV/μRIU, 0.8mV/μRIU)8μL1.0~50mL/min100mL/min (solvent ; pure water)IN Cell : ca. 120μLCell OUT : ca. 510μLAll (Cell OUT) : ca. 640μLInstruments Refractive Index Detector91Recorder outputExternal inputExternal outputTemperature controlCommunication portOperational support functionsWetted materialsPower source, Power consumptionDimensions, WeightAccessories*Pure water 1mL/min, PURGE OFF**Pure water, response : 1.5sec0~10mV/FSPurge On/Off, Auto Zero, MarkerREADY (Automatic start-up)LEAKERROR (OVER HEAT/LOW LIGHT INTENSITY/NULL GLASS HOME POSITION/LOST PARAMETERS/OPTICAL BALANCE) (Contact capacity : DC24V 0.1A max.)OFF, 30~55˚C (1˚C step), 77˚C Temp. fuseRS232CAutomatic start-up (Start Up Sequence) Span/Validation Guide Real Time Baseline MonitorStainless steel 316, Teflon, Quartz glassAC100~240±10%, 50/60Hz, 150VA maxW260 x D400 x H200 (mm), ca.13kgPower cable, signal cable, connector tube, fuse, operation manual

Instruments Electric Conductivity Detector Dissolved Gas Removal Devices92NEWProduct CodeModelMeasurement methodMeasurement limitMeasurement rangeLinearity rangeResponseAuto zero limitBaseline shiftIntegrator outputRecorder outputExternal inputExternal OutputCell Temperature controlCommunication portCell volumePressure ratingWetted materialsDimension, WeightPower source,Power consumptionElectric Conductivity DetectorShodex CD−200The electric conductivity detector is designed for ion chromatography. It is recommended for anion orcation analysis in aqueous solution.FeaturesWith use of the built-in double temperature control cell, thisdetector enables highly sensitive measurement.The detector supports a wide range of measurement and isusable for ion chromatography with or without suppressormethod.F5515010CD-200Two-electrode system0~600mS/m (0~6mS/cm)0.0025~5.12mS/m, 0.025~51.2mS/m, 0.25~512mS/m600mS/m0.1, 0.25, 0.5, 1.0, 1.5, 2, 3, 6secSame as measurement limitRange;0~2mS/m, Resolution;0.01mS/m0~1V (Sensitivity:200, 20, 2mV/mS/m)0~10mV/FSZERO INMARKER INREADY(TEMPERATURE STABILIZED)LEAKERROR(ROM,RAM,PARAMETER,SENSOR,OVER HEAT,ZERO OVER)MARKER OUTOFF, 30~50˚C (1˚C step), 77˚C Temp. fuseUSB2.5μL1MPaStainless steel 316, Teflon, PEEKW260 x D400 x H150 (mm), ca. 8kgAC 100~240V±10%,200VA max(OUT)HeatexchangerDissolved Gas Removal DevicesDEGASSER ERC-3215α, 3415αThe ERC-3000α series efficiently remove dissolved gases in the eluent.Degassing principleA special synthetic resin membrane (degassing membrane) isused to selectively remove dissolved gasses ; using thecharastaristics of the small molecular size of the dissolved gaswith high mobility and affinity to the membrane.FeaturesPressure sensor and leak monitor assure a high degree of safety.Dead volume is minimized to 7 ml/flow channel.Any pump can be used since differential pressure is low.Continuous degassing mode aids eluent preparation forhigh-sensitivity analysis.EMC and LVD compliance, with CE marking.Principle of measurement of the electric conductivity detectorEluent(IN)ElectrodeSpacerElectrodeExternal cylinder(temperature control at presettemperature - 5°C)Internal cylinder(temperature control at presettemperature)Current/voltageconvertercircuitAC voltagegeneratorSynchronousrectifier circuitSignalprocessingcircuitElectricconductivitysignalProduct CodeModelSolvent ChannelsDegassingcapacityInternal volumeMax. Flow RateExternal outputDimensionsPower sourceFunctions,DisplaysWeightY4617000ERC-3215α2 channelsY4617004ERC-3415α4 channelsWhen ion-exchanged water saturated with air at 25˚C is put through at aflow rate of 3 mL/min, no bubble is observed at the outlet of the apparatus.(Measured dissolved oxygen level at the outlet: 2 ppm at flow rate of 3 mL/min)7mL/Channel20mL/min for each channel (Eluent : 25˚C Pure water)An open connector signal is delivered to the external outputsignal terminal, when "PRES" and "LEAK" LEDs lights are on.W71 x D310 x H136 (mm)AC100V~AC240V, 50/60Hz• Power On/Off display : "POWER" LED lights, when the power is supplied.• Status monitoring function : "READY" LED lights, when the internalpressure in the vacuum chamber is below a predetermined limit.• Pressure monitoring function : "PRES" LED lights, when the internalpressure in the vacuum chamber does not reach a predetermined levelwithin a predetermined time.• Leak monitor : "LEAK" LED lights, when the liquid leaks in the apparatus.• Self cleaning : The vacuum line is cleaned by air suction.• Vacuum pump operation switching function:NORM. : Controlled operation in normal runCONT. : Continuous operation in case of high degree of degassingca. 5.1kgca. 5.5kgVacuum chamberMembrane degasserFeed pump tooutletEluentPressuresensorVacuum pumpControlboardThree-waysolenoid valve•In addition to this product, various degassers are availableincluding the six-flow channel type and the high flow ratetype. For details, please contact Shodex or our distributorsnear you.

Instable baseline valuesSTARTColumn PressurenormalYESBaseline stable whenpump stoppedYESLeakage observedYESFlow rate fluctuationHPLC System Trouble ShootingNONONOCommon cause of abnormal chromatogramsYESMonotonic driftNO······Main cause······SolutionMalfunction of check valveTemperature fluctuationDeterioration of detector lampInsufficient groundingMalfunction of recorderBubbles in flow cellContamination of flow cellInsufficient eluent replacement(with different solvent, salt or others)Contamination of columnPulsation of pump······Frequent problem· Replace check valve· Clean up check valve· Warm up detector sufficiently· Shield detector from wind· Check column oven· Check detector temperature controlReplace lampEstablish sufficient groundingRepair recorderWash inside of detectorWash inside of detectorWash flow line of HPLC· Wash column· Check column performance· Replace columnUse damperInformation HPLC System Trouble Shooting93Tighten connectionsDeterioration of eluent· Use eluent containing stabilizer(for THF, use THF containing BHT)Noise······Main cause······Solution······Frequent problemSTARTInsufficient groundingEstablish sufficient groundingBaseline stable whenpump stoppedNOIrregular noiseDeterioration of detector lampReplace detector lampYESMalfunction of recorderRepair recorderShodex DEGAS usedNOInsufficient degassing eluentUse Shodex DEGASYESSpike noiseYESOKSpike noise foundfrom beginingNOYESAir in columnProblem solved by applying backpressure to column(*)(*) Do not apply back pressure to detectorColumn temperature exceeding eluent boiling pointNO· Check column performance· Replace columnReduce temperature of columnoven and/or post-column reactioncoilNoise shape leadingNOContamination of flow cellWash flow cellYESBubbles in flow cellRepair detector

Information Index by product name94IndexColumns are listed in alphabetical order under the product name excluding series name.Instruments are indicated by bold letters.[Series name]AFpakIECSilicaAAAB-894AAF-894AAM-894AAP-894AAV-894ABA-894ABT-894ACA-894ACB-894ACH-494ADS-894AED-894AGA-894AGE-894AGO-494AGT-894AHR-894AIA-894ALC-894ALS-894ANA-894AOV-894APA-894APB-894APD-894APG-894APH-894APR-894ARC-894AST-894AT-806MS, AT-GAWG-894CAsahipakMSpakSilicapakC18MC18PC4P-50, C4P-50G 4A5C45C8C8P-50, C8P-50G 4ACARB-413, CARB-413LCARB-LFCD-200CDA-453 HQCDB-453 HQCDBS-453CDC-453 HQCD-GCM-8255CNCRX-853, CRX-GAXpakODSpakSTANDARDCLNpakOHpakSTD28, 29, 3028, 2928, 2928, 2928, 29, 3022, 23, 28, 2928, 2928, 29, 3028, 2917, 28, 2928, 29, 3028, 2928, 2928, 2928, 2928, 2928, 2928, 2928, 29, 3028, 2928, 29, 3028, 2928, 2928, 2928, 2928, 2928, 2928, 2928, 2928, 29, 3058, 5928, 2920, 2120, 2110, 14202010, 1412, 17129222, 2322, 2322, 2322, 232226, 272022, 23CXpakORpakSUGARDGPCPIKESSUSPpakDC-613, DC-GDE-213DE-413DE-613DE-G, DE-SGDEAE-2BDEAE3N-4TDEAE-825DM-614, DM-GDS-413, DS-613, DS-GEE-411ET-RP1ERC-3215α, 3415αES-502C 7CES-502N 7CEV-200EV-2000, EV-GEV-2000 AC, EV-G ACFF-411, F-511, F-411/SFL-1, FL-1 filterFL-2, FL-2 filterGGF-1G 7BGF-310 HQGF-310GF-4AGF-510 HQGF-710 HQGF-7M HQGOLF-413GS-220 HQGS-2G 7BGS-310GS-320 HQGS-320GS-510GS-520 HQGS-520GS-620 HQGS-620GS-710GSM-700HHICPROTEINWINEHFIP-603~607, HFIP-606M, HFIP-GHFIP-803~807, HFIP-806M, HFIP-LGHLDHT-803~806, HT-806M, HT-GICRSpak33, 351111, 16, 1711, 16, 1711, 172424, 2524, 251111, 162011, 169326, 2724, 25666, 720, 2138, 4038, 4050, 8850, 5150850, 5150, 5150, 511248, 4948, 888848, 4948, 49, 888848, 498848, 4948, 49, 88888860, 6160, 61858, 59

II-524A, IA-GJJJ-50KK-801~807, K-803L~807L, K-806M, K-GK-800DKC-811, KC-G, KC-LGKD-801~807, KD-806M, KD-GKF-401HQ~404HQ, KF-405LHQ, 406LHQKF-601~607, KF-606MKF-800DKF-801~807, KF-803L~807L, KF-806MKF-GKS-801~806, KS-GKS-807, KS-807GKW402.5-4F~405-4F, KW400G-4AKW-604SKW-802.5~804, KW-GLLF-404, LF-604, LF-804, LF-GMM-75MN-431N5NHNH2P-40NH2P-50, NH2P-50GNH2P-LFNI-424, NI-GNN, NN-G5NPE 4DOODP2 HPODP-40ODP-50, ODP-50GQ38, 39 QA-82524, 2512, 1856, 57, 6252, 5436, 3754, 55, 6256, 57, 6256, 57, 6252, 54, 5552, 53, 625232, 33, 343244, 458, 944, 4558, 59, 626332, 352013, 1813, 18, 191338, 3912, 17, 1820, 2111, 151010, 14, 15RR-621, R-GRP18-413, RP18-415, RP18-613, RP18-GRI-101, RI-102, RI-104RI-201, RI-201HSSB401-4E~404-4E, SB400G-4ASB-802 HQ~806 HQ, SB-806M HQ, SB-GSC1011, SC1821, SC-LGSC1211, SC-GSH1011, SH1821, SH-GSH-75SI-50 4ESI-52 4ESI-90 4ESI-91 4CSI-50G, SI-90G, SI-92G5SILSL-105SM-105SP0810, SP-GSP-2BSP-420NSP-825SZ5532, SZ-GTT-521, T-GUUT-802.5, UT-806M, UT-807, UT-GVVH-anion 4D, VH-anionG 4AWWA-624, WA-G4011, 1691904646, 4732, 33, 3433, 3536, 376338, 3938, 3938, 39383820, 21636332, 33, 34, 3526, 2726, 2736, 3733, 3540, 4158, 593824, 25Information Index by product name95PP-421S, P-GP-82PAE-2000, PAE-GPAE-2000 AC, PAE-G ACPAE-800PAE-800 ACPH-814PK-2A, PK-4A5PYE 4D26, 276366, 766, 726, 278, 920, 21YY-521, Y-521L, Y-GYK-421, YK-GYS-50, YS-G40, 4140, 4140, 41

Technical informationlistTechnical Notebook, Chromato NewsNotebookNo.1234Technical NotebookTitleShodex GPC LF series columnsLinear calibration curves over a wide molecular weight rangeShodex NH2P-50 series columnsAnalysis of saccharides in food industryFood Analysis with Shodex columnsSaccharides, Organic acids, Vitamins, Fatty acids and Amino acidsShodex IC YS-50 columnSimultaneous analysis of monovalent and divalent cations at ahigher sensitivityNo.567TitleShodex KW400 series columnsHigh performance and downsized column for protein analysisShodex ODP2 HP series columnsBetter retention of highly polar substancesProtein & Peptide with Shodex columnsNewsChromato News96Technical Notebook, Chromato NewsNo. Title Column12345678High performance reversed phase column; polymer-based column Asahipak ODP-40Direct analysis of medical compounds in serum without sample pretreatmentSemi-micro GPC columns with high resolutionLinear calibration curves by GPC LF-804New IC columns for suppressed ion chromatographyFood additivesPolymer based column with multimode of reversed phase and ionic interactionUltra-high molecular weight and water-soluble polymer with Shodex SUGAR KS-807Asahipak ODP-40 seriesMSpak GF-310 series, MSpak GS-320 seriesGPC KF-400HQ seriesGPC LF-804IC SI-90 4E, SI-50 4ESUGAR SP0810, SC1011, Asahipak NH2P-50 4E, RSpak KC-811RSpak NN series, RSpak JJ-50SUGAR KS-807, KS-800 series91011121314151617181920212223242526272829303132333435363738394041424344Direct analysis of proteins and peptides in surfactants using surfactant removalcolumn MSpak PK seriesDirect analysis of additives in polymer with SEC/MSConcentration of the hydrophilic medical compounds existing with proteinsColumn selection for proteins and peptides analysisAnalysis of Hyaluronic acid with ultra-high molecular weight in serumWater-soluble vitaminsAnion analysis with non-suppressor methodSEC analysis of proteins with PROTEIN KW-800 seriesSaccharides and sugar alcohols with Asahipak NH2P-50 columnAnalysis of quite small amount of sample with micro-columnNew micro column seriesNew micro column series; similar separation with standard column and higher sensitivityAffinity column seriesOnline removal of surfactants in protein analysisVarious application with Repack DE series; high pH, 0% organic eluentAnalysis of amines and amides with RSpak DE seriesSimultaneous analysis of monosaccharide and organic acid with SUGAR SH1011ORpak CDBS-453; Chiral column for the analysis of drugs and their metabolitesAnalysis of chiral medicine warfarin and its metabolites with CDBS-453Cations analysis column IC YS-50 for Suppressor and Non-suppressor systemWater-soluble ultra-high Molecular compounds with OHpak SB-807HQNew reversed phase column ODP2 HPNew protein analysis column KW400High sensitive analysis of pyridylaminated saccharidesNew rapid cation exchange column; DEAR3N-4T with nonporous gelOxyhalides and cyan in drinking watersShellfish poisoning and pesticidesSuitable column for LC/MS application; ODP2 HPIon chromatography for wine analysisHigh performance SEC columns SB400 for water soluble polymer in small dimensionIonic polymer analysis with SB seriesAnalysis of sugars in bioethanol productionHigh-temperature reversed phase column in high temperature for rapid analysisIon Exchange columns for UHPLCSaccharides analysis with H 2 O eluentHigh resolution separation by NH2P-40 3EMSpak PK series, MSpak GF-4AGPC KF-400HQ seriesMSpak PK seriesSEC, Reversed phase, Ion exchange, Affinity columnsSUGAR KS-807, Asahipak GS-620HQAsahipak NH2P 50 4E, RSpak DE-413IC NI-424PROTEIN KW-800 seriesAsahipak NH2P-50 seriesShodex micro column seriesGS320A-M5D, GS520A-M5DGS-320 HQ, GS320A-2E, GS320A-1EAFpak seriesMSpak PK-2A, PROTEIN KW-604SRSpak DE seriesRSpak DE-613, DE-413, AFpak ACH-494SUGAR SH1011ORpak CDBS-453ORpak CDBS-453IC YS-50, IC YK-421OHpak SB-807HQ, SB-806HQODP2 HP seriesKW400 seriesAsahipak NH2P-50IEC DEAE3N-4TIC SI-52 4E, IC SI-91 4C, RSpak KC-811 6ERSpak DE413-2B, Asahipak ODP-50 4EODP2 HP-LCMSWINE VH-anion 4DOHpak SB400 seriesSB400 series, OHpak SB-803 HQSUGAR SH1821, SUGAR KS-802, GPC LF-804ET-RP1 4DPIKESS seriesSP0810, SC1011, KS-801, SH1011NH2P-40 3E

Useful Information Regarding ShodexShodex HPLC Columns and instruments are manufactured in Japan by Showa Denko K.K.Showa Denko Europe GmbH is a wholly owned subsidiary of Showa Denko K.K.Shodex ® is a trademark of Showa Denko K.K.Visit us on our European Website atwww.shodex.dein 5 languages.* For technical support and applications* For information on new products and events* For newsletter subscription* For literature* For purchasing enquiriesTechnical SupportTEL : (+49) 089 93 99 62 37E-mail : info@shodex.deShowa Denko Europe GmbHKonrad-Zuse-Platz 4, 81829 Munich, GermanyOrderingTEL : (+49) 089 93 99 62 34E-mail : order@shodex.deShowa Denko Europe GmbHKonrad-Zuse-Platz 4, 81829 Munich, Germany

Please contact Shodex support office near youSupport officeASIA & PACIFICKOREACHINAShowa Denko K.K.Shodex (Separation & HPLC) Group5-1, Ogimachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0867, JAPANTEL : +81-(0)44-329-0733 FAX : +81-(0)44-329-0794Email : sdk_shodex@sdk.co.jpShoko Korea Co., Ltd322ho, Chungjeong Rezion Bldg, 465, Chungjeongno3-ga,Seodaemun-gu, Seoul, 120-013, Korea.TEL : +82-(0)2-784-5111 FAX : +82-(0)2-784-5125Email : shoko.korea@shokokorea.comURL : www.shodex.com/index_kr.htmlShodex China Co. Ltd.18F, Wang Wang Building, No.211 Shi Men Yi Road, Jing An,Shanghai, 200041, CHINATEL : +86-(0)21-6217-6111 FAX : +86-(0)21-6217-9879Email : support@shodexchina.comURL : www.shodex.com/index_ch.htmlNORTH &LATIN AMERICAShowa Denko America, Inc.420 Lexington Avenue, Suite 2850, New York, NY 10170 USATEL : +1-212-370-0033 FAX : +1-212-370-4566Email : support@shodex.netURL : www.shodex.netEUROPE & AFRICA Showa Denko Europe GmbHKonrad-Zuse-Platz 4, 81829 Munich, GERMANYTEL : +49-(0)89-93996237 FAX : +49-(0)89-93996250Email: info@shodex.deURL : www.shodex.dehttp://www.shodex.deManufactured bySHOWA DENKO K.K.Shodex (Separation & HPLC) Group5-1, Ogimachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0867 JAPANTEL : +81-(0)44-329-0733 FAX : +81-(0)44-329-0794CAT.NO.01E.(1)11.****.JAN.TR