Asahipak - Hplc.eu

Standard analysiscolumnsAmino acid analysis using multimodeSample : 20μL1. Aspartic acid2. Glycine3. Alanine4. Valine5. Methionine6. Isoleucine32Haloacetic acid analysis using multimodeSample : 500mg/L each, 2μL1. Chloroacetic acid2. Bromoacetic acid3. Dichloroacetic acid4. Dibromoacetic acid5. Trichloroacetic acid4Phenylenediamine isomers analysis usingmultimodeSample : 100mg/L each, 20μL1. p-Phenylenediamine2. m-Phenylenediamine3. o-Phenylenediamine121423563510 20 40 60 80 min0 5 10 15 20 min0 5 10 15 min18Polymer-based Packed Columns for Reversed Phase ChromatographyPolymer-based Packed columns for Hydrophilic Interaction Chromatography (HILIC)Column : Shodex RSpak NN-814Eluent : 40mM Phosphoric acid aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CAscorbic acid and erythorbic acidSample : 5μg/mL each, 10μg1. Erythorbic acid 2. L-Ascorbic acidO=CO=CHOCHOCOOHOCHOCHCHCHCOH1 HOCHCH2OHCH2OH2600000400000200000215001000Column : Shodex RSpak JJ-50 2DEluent : 25mM Ammonium acetate buffer(pH9.2)/CH 3 CN=50/50Flow rate : 0.2mL/minDetector : UV(210nm)Column temp. : 40˚CErythorbic acidR 2 =0.99990 200 400Erythorbic acidL - Ascorbic acidR 2 =0.9997NH2P-50 4EFirst use13214Sucrose14Sucrose5001212L - Ascorbic acid10Glucose10Glucose8Fructose8Fructose00 5 10 min0 0.5 150 100 150 20050 100 150 200Sample concentration(μg/mL)Time (hr)Time (hr)Column : Shodex Asahipak NH2P-50 4EColumn : Shodex Asahipak NH2P-50 4E, Silica based amino column (company-A)Eluent : 20mM NaH 2 PO 4 + 30mM H 3 PO 4 (pH2.2)/CH 3 CN=20/80Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minFlow rate : 1.0mL/minDetector : UV(254nm)Detector : RIColumn temp. : 30˚CColumn temp. : 30˚CComparison between NH2P-40 3E and NH2P-50 4ENH2P-40 3E (New)(3.0mmI.D. x 250mm)Peak height(μV)Peak height(μV)Sample concentration(μg/mL)NH2P-50 4E (existing)(4.6mmI.D. x 250mm)0 1020181641. Fructose2. Glucose3. Sucrose4. MaltoseColumn : Shodex RSpak JJ-50 4DEluent : 25mM Ammonium acetate buffer(pH9.2)/CH 3 CN=70/30Flow rate : 0.4mL/minDetector : UV(254nm)Column temp. : 30˚CRepeated analysis with NH2P-50 column and silica-based amino columnFor every kind of saccharide, the resistance to time degradation of our polymer-basedamino column NH2P, enables to maintain a stable retention time and obtaining sharppeaks.Ve (mL)After 180hrs1321. Fructose12. Glucose3. Sucrose 2 34 4. Maltose420 min 0 10 20 min 0 10 20 min 0 10 20 minMaltoseSample : 0.5% each, 4μL1. Fructose2. Glucose3. Sucrose4Silica-based amino columnFirst use1Ve (mL)20181632MaltoseAfter 120hrsThe NH2P-40 3E is a polymer-based amino column (3 mm inner diameter)packed by downsizing particle size. This general-purpose columnperform higher resolution and separation of the conventional column(NH2P-50 4E).1TPN (Sucrose)Fructose/GlucoseRsGlucose/SucroseNH2P-40 3E11,3006.48.5NH2P-50 4E8,7004.25.92312305 10 15 20 0 5 10minmin15 20Column : Shodex Asahipak NH2P-40 3E, NH2P-50 4EEluent : H 2 O/CH 3 CN=25/75Flow rate : (NH2P-40 3E) 0.35mL/min, (NH2P-50 4E) 1.0mL/minDetector : RIColumn temp. : 25˚C