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Calibration curves for KD-800 seriesusing PEG/PEO standardMolecular weight (PEG/PEO)10 710 610 510 410 3KD-805KD-804KD-803KD-802.5KD-807KD-806KD-802KD-806MPolyvinylpyrrolidoneSample : Polyvinylpyrrolidone(PVP) 0.2% each(A) PVP(K-30), 400μL(B) PVP(K-60), 500μL(C) PVP(K-90), 500μL(A)(B)Melamine formaldehyde resinSample : 30μLMelamine-formaldehyde resin 1%Standard analysiscolumns10 2KD-801(C)1010 8 Elution volume (mL)6 7 8 9 10 11 120 10 20 30min10152025 minColumn : Shodex GPC KD-800 seriesEluent : DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CCelluloses10 610 510 410 310 7 6 78 9 10 11Column : Shodex GPC KD-806MEluent : 1% LiCl in DMIFlow rate : 0.5mL/minDetector : MALS, RIColumn temp. : 60˚C* DMI 1,3-dimethyl-2-imidazolidinonePotato starchSample : 100μLPotato starch in DMSO 0.1%* solved at 80˚C0 5 10 15 minColumn : Shodex GPC KD-806MEluent : 10mM LiBr in DMSO/DMF=75/25Flow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CColumn : Shodex GPC KD-806M x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚C0Sample : Cellulose ca. 0.05% each100μLEffects of solvent-peak separation columnSample : Polyvinylchloride(1)KF-806L(2)KF-806L + KF-800DCellulose is known to be difficult todissolve. A cellulose solution isprepared by repeating solventreplacement. It is reported that thelong time required for dissolution(1 to 60 days), which depends onsolvent type, the crystallinity andmolecular weight of the sample.Data provided by Mr. Masahiro Yanagisawa,Isogai Laboratory,Graduate School of Agricultural and Life Sciences,University of Tokyo.5 10 15 min0 5 10 minDOP(Dioctylphthalate)Molecular weight (Polystyrene)Column : Shodex GPC KD-802 x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CPhenoxy resin1520minColumn : Shodex GPC KD-806M x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚C10 510 410 310 2 5 6 7 8 9 10 11 12 13 14 1510 6 Retention volume (mL)KF-806LKF-806L+KF-800DSample : 250μLPhenoxy resin 0.2%Column : (1) Shodex GPC KF-806L(2) Shodex GPC KF-806L + KF-800DEluent : THFFlow rate : 1.0mL/minDetector : RI2555Organic SEC (GPC) columns (General Analysis) : DMF Solvent-peak separation columns for organic SEC (GPC)