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Anion analysis with non-suppressor method (NI-424 and I-524A)NI-4241234 5 67Sample : 50μL1. H2PO - 4 20mg/L2. F - 1.5mg/L3. CI - 2.5mg/L4. NO2 - 6mg/L5. Br - 10mg/L6. NO3 - 10mg/L7. SO4 2- 10mg/LI-524A1,234567NI-424 is a high-performance columnwith a theoretical plate number whichis approximately twice as large asthan that of I-524A.(1) Enables the separation of H 2 PO 4-and F - ions which were difficult toseparate with I-524A.(2) The shape of each peak is sharper,and the separation balance isfavorable. Improvements havebeen made especially in theseparation of Cl - and NO 2- ions.Standard analysiscolumns0 2 4 6 8 10 12 14 16 min02 4 6 8 10 12 14 min0Column : Shodex IC NI-424Eluent : 8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris+ 2mM Phenylboronic acid + 5μM *CyDTA(pH4.2)Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C*CyDTA : trans-1,2-Diaminocyclohexane-N,N,N',N'-tetra acetic acidAnion analysis with suppressor method using SI-90 4E1235456710 min8Sample : 20μL1. F - 2mg/L2. Cl - 3mg/L3. NO2 - 5mg/L4. Br - 10mg/L5. NO3 - 10mg/L6. HCO3 - 300mg/L7. PO4 3- 15mg/L8. SO4 2- 15mg/LColumn : Shodex IC I-524AEluent : 2.5mM Phthalic acid + 2.3mM Tris(pH4.2)Flow rate : 1.2mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CAnion analysis with suppressor method using SI-50 4ESI-50 4E is a high-performance version of SI-90 4E.Acetic acid, formic acid, and methacrylic acid areeluted later than F - ion and faster than CI - ion. Thecarbonate system peak appears between NO 2- andBr - peaks.12 3456780 5 10 15 20 25 30 min910Sample : 20μL1. F - 2mg/L2. Acetic acid 10mg/L3. Formic acid 2mg/L4. Methacrylic acid 10mg/L5. Cl - 3mg/L6. NO2 - 5mg/L7. Br - 10mg/L8. NO3 - 10mg/L9. PO4 3- 15mg/L10. SO4 2- 15mg/L11. Oxalic acid 15mg/L1139Columns for Ion Chromatography (Anion analysis)Column : Shodex IC SI-90 4EEluent : 1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.Flow rate : 1.2mL/minDetector : Suppressed conductivityColumn temp. : 25˚CColumn : Shodex IC SI-50 4EEluent : 3.2mM Na 2 CO 3 + 1.0mM NaHCO 3 aq.Flow rate : 0.7mL/minDetector : Suppressed conductivityColumn temp. : 25˚COxyhalides analysis with suppressor method using SI-52 4E145 691011Sample : 50μL1. F - 2mg/L2. ClO2 - 1mg/L3. BrO3 - 1mg/L4. Cl - 10mg/L5. NO2 - 5mg/L6. Br - 10mg/L7. ClO3 - 1mg/L8. Dichloroacetate 1mg/L9. NO3 - 30mg/L10. PO4 3- 15mg/L11. SO4 2- 40mg/LSI-52 4E is a high-resolution column exhibiting a number oftheoretical plates of 14,000 or higher. It supports simultaneousanalysis of Oxyhalides and inorganic anions.The recommended temperature is 45˚C.Calibration curves of Oxyhalides with the SI-52 4Epeak area (μs/cm*sec)2.521.510.50ChloriteR 2 =0.994910 20 30 40 50 60 70 80 90 100ClO 2- concentration(μg/mL)peak area (μs/cm*sec)21.81.61.41.210.80.60.40.200ChlorateR 2 =0.999510 20 30 40 50 60 70 80 90 100ClO 3- concentration(μg/mL)2 37 805 10 15 20 25 30 minColumn : Shodex IC SI-52 4EEluent : 3.6mM Na 2 CO 3 aq.Flow rate : 0.8mL/minDetector : Suppressed conductivityColumn temp. : 45˚Cpeak area (μs/cm*sec)Bromate1.41.2R 2 =0.998510.80.60.40.200 10 20 30 40 50 60 70 80 90 100BrO - 3 concentration(μg/mL)