Asahipak - Hplc.eu

Calibration curves for GF-HQ series usingpullulan and PEGMolecular weight10 6 Elution volume (mL)10 510 410 3GF-510 HQGF-310 HQGF-710 HQGF-7M HQPullulanPEGCalibration curves for GF-HQ series usingpolystyreneMolecular weight (Polystyrene)10 6 Elution volume (mL)10 510 410 3GF-7M HQGF-310 HQGF-710 HQGF-510 HQPolyacrylonitrileSample : Polyacrylonitrile 0.1%(w/v), 100μLStandard analysiscolumns10 2 4 5 6 7 8 9 10 1110 2 4 5 6 7 8 9 10 110 5 10 15 20 25 30 35 40 minColumn : Shodex Asahipak GF-HQ seriesEluent : H 2OFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CComparison of standard polystyrene separation using various solventSample : 1mg/mL each, 50μLPolystyrene1. MW : 1,090,0002. MW : 190,0003. MW : 37,9004. MW : 9,1005. MW : 2,9806. MW : 500(A) THF (B) Chloroform (C) DMF12345 6 12345 6 1Column : Shodex Asahipak GF-HQ seriesEluent : ChloroformFlow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚C23 4 56Sodium polystylene sulfonateMW:104,0000 10 20 30 minMW:38,0000 10 20minColumn : Shodex Asahipak GF-710 HQ x 2Eluent : 20mM LiBr in DMFFlow rate : 0.6mL/minDetector : RIColumn temp. : 40˚CPolymers having both hydrophobic andhydrophilic moieties may exhibithydrophobic interactions with packingmaterials. When using such polymers,SEC analysis can be performed byeliminating the hydrophobic interactionthrough the addition of organic solventsto the eluent.Sample : 1mg/mL each, 50μLSodium polystylene sulfonateCH2CHSO3Nan51Aqueous/Organic SEC columns0 20 min 0 20 min 0 20 minColumn : Shodex Asahipak GF-310 HQ + GF-510 HQEluent : (A); THF, (B); Chloroform, (C); DMFFlow rate : 0.5mL/minDetector : (A),(B) UV(254nm), (C) UV(270nm)Column temp. : 30˚C0MW:8,00010 20 30 minColumn : Shodex AsahipakGF-510 HQEluent : 50mM LiCl aq./CH 3 CN=60/40Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚CTriglyceridesNonylphenol ethoxylate12Sample : 0.25% each, 20μL1. Tristearin2. Trilaurin3. Tributylin4. TriacetinGF-310 column is suitable for qualitative analysis andquantitative analysis of surfactants to be performed byadjusting the acetonitrile concentration in the eluent.(A) Eluent : H 2 O/CH 3 CN=70/30Sample :100mg/L Nonylphenol ethoxylate, 20μL(B) Eluent : CH 3 CN3451015 min0 20 40 60 80 min 5 10 15 20 minColumn : Shodex Asahipak GF-310 HQEluent : THFFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CColumn : Shodex Asahipak GF-310 HQEluent : (A); H 2 O/CH 3 CN=70/30, (B); CH 3 CNFlow rate : 0.6mL/minDetector : UV(220nm)Column temp. : 40˚C