Asahipak - Hplc.eu

Hydantoins3214RONONHSample :Hydantoins, 0.05% each, 3μL1. 5-Carboxyethyl hydantoin2. 5-Hydroxymethyl hydantoin3. Hydantoin4. 5-Methyl hydantoin5. 5-Ethyl hydantoinCholine and acetylcholine13Sample : 10μL1. Choline 5mg/L2. Ethylhomocholine 10mg/L3. Acetylcholine 10mg/LDegasPumpDE-413ACH-494ECDStandard analysiscolumnsColumn oven5Eluent0 2 4 6 8 minColumn : Shodex RSpak DE-613Eluent : 1/30M Na 2 HPO 4 + 1/30M KH 2 PO 4 aq.Flow rate : 2.0mL/minDetector : UV(210nm)Column temp. : 50˚CAnalysis of diarrheal and fat-soluble shellfish poisoningOkadaic acidSIM : 803.5PTX2(Pectenotoxin-2)SIM : 903.5DTX3(Dinophysistoxin-3)SIM : 1055.7N-methylcarbamate pesticides1234InjectorPumpDegasserEluentDE-G5CARB-4136PumpNaOH aq.reagent790˚CReaction coilPumpOPAreagentreagent8PTX6(Pectenotoxin-6)SIM : 887.5DTX1(Dinophysistoxin-1)SIM : 817.5PTX1(Pectenotoxin-1)SIM : 919.540˚CReaction coilFluorescencedetectorSample : 0.1ppm each, 20μL1. Oxamyl2. Methiocarb sulfoxide3. Aldicarb4. Methiocarb sulfone5. Bendiocarb6. Ethiofencarb7. Carbaryl8. Fenobucarb9. Methiocarb920 510 minMorphology of arsenic compounds200010002000digestive cecum of shellfish,1g90% Methanol, 9mLhomogenize (3-5 min)inject into LCMS 5uL ofsupernatant0YTX(Yessotoxin)SIM : 1141.5Sample pretreatmentcentrifuge (3,000rpm, 5min)Standard arsenic compounds12 3 425 6100 200 300 400 500 600 700 800 900 10001100 1200Urine from an arsenicpoisoning patientColumn : Shodex RSpak DE-413Post column : Shodex AFpak ACH-494Eluent : 0.1M Phosphoric acid +300mg/L Sodium 1-decansulfonate +65mg/L Tetramethylammonium chloride(pH8.0 adjusted by 1M NaOH)Flow rate : 1.0mL/minDetector : EC(Electrode: Pt, 350mV SCE)Column temp. : 37˚CColumn : Shodex DE413-2BEluent : (A); 50mM HCOOH + 2mM HCOONH 4 aq.(B); 50mM HCOOH + 2mM HCOONH 4 in(H 2 O/CH 3 CN=5/95)0 to 5min, 20% (B)5 to 12min, 20% (B) to 100% (B)Flow rate : 0.2mL/minDetector : ESI-MS(Negative)2000* See p.28 for ACH-494While a toxicity testing using mice is a mainstreammethod for examination of shellfish poisoning, ananalytical method using LC/MS was developed tomeet the efficient and high-performance shellfishpoisoning examination to cope with the longinspection time and variance in laboratory resultsof the conventional inspection method, and toreduce the use of animals. The use of LC/MSenabled rapid detection of components which aredifficult to be detected in an animal study.Data provided by Mr. Toshiyuki Suzuki of the Fisheries Research AgencySample : Arsenic compounds, 50μL1. Monomethylarsonic acid2. Arsinic acid3. Dimethylarsinic acid4. Arsenobetaine5. Tetramethylarsonium6. Trimethylarsine oxideUrine of a healthy person417Polymer-based Packed Columns for Reversed Phase Chromatography1000410000 10 20 30 minColumn : Shodex RSpak CARB-413Eluent : CH 3 CN/H 2 O=37/63Flow rate : 0.8mL/minReagent : (A); 50mM NaOH(B); 0.05% o-Phthalaldehyde + 0.1% MercaptoethanolDetector : Fluorescence(Ex. : 340nm, Em. : 450nm)Column temp. : 40˚C130 100 200 300 400 500 600 700 800 900 1000 1100 1200Column : Shodex RSpak NN-614Eluent : 5mM HNO 3 /8mM NH 4 NO 3 aq.Flow rate : 0.8mL/minDetector : IC-ICP-MS(SIM m/z=75)1235 6100 200 300 400 500 600 700 800 900 100011001200Noriko TSUNODA,National Reseach Institute of Police Science,Pharmacia, vol.34 No.12 (1998), p1237-12421