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InformationApplicability of SEC (GPC) columns to solventreplacementColumn NameShipping Solvent : THFShipping Solvent : DMFSolventKF-801KF-802KF-802.5KF-803LKF-804LKF-803KF-804KF-805KF-806KF-807KF-806MKF-805LKF-806LKF-807LKF-601KF-602KF-602.5KF-603KF-604KF-605KF-606KF-607KF-606MLF-804LF-604LF-404KD-801KD-802KD-802.5KD-803KD-804KD-805KD-806KD-807KD-806M62Applicability of SEC (GPC) columns to solvent replacementTHFChloroformCarbon tetrachlorideBenzeneToluenep-Xyleneo-Dichlorobenzene(ODCB)Trichlorobenzene(TCB)DioxaneDiethyl etherEthyl acetateAcetoneMethyl ethyl ketoneDimethylformamide(DMF)Shipping Solvent : ChloroformK-801 K-802 K-803 K-804K-802.5K-805K-803LK-806K-804LK-807K-806MK-805LK-806LK-807LShipping Solvent : THFKF-401HQ KF-403HQKF-402HQ KF-404HQKF-402.5HQ KF-405LHQKF-406LHQ*1*1*1Dimethylacetamide(DMAc)*1*1*1Hexafluoroisopropanol(HFIP)*1*1m-Cresolo-ChlorophenolQuinolinN-Methylpyrrolidone(NMP)*1*1*1Dimethylsulfoxide(DMSO)*1*1*130% m-Cresol/Chloroform30% o-Chlorophenol/Chloroform30% HFIP/ChloroformHexaneAcetonitrileMethanolWater: Solvent replacement possible: Solvent replacement possible, but this may cause column performance to slightly deteriorate*1 : Usable at 40˚C or higher: Solvent replacement not possible
Calibration Standards for SEC[Polystyrene (PS)]FeaturesFor organic solvent SEC (GPC)Less branched polystyrene with anionic polymerizationEasily soluble in tetrahydrofuran (THF), chloroform, toluene, and o-dichlorobenzeneKit typeProduct CodeF8601105Product Name Contents MW RangeSTANDARD SL-1050.5g x 10 kinds500~22,000Structural formula of S seriesC 4H 9 CH 2 CH HCalibration standardsfor SECF8602105STANDARD SM-1050.5g x 10 kinds1,200~3,800,000nF8603075STANDARD SH-750.5g x 7 kinds590,000~7,500,000SL-105 SM-105 SH-105Std. No. Mp Mw/Mn Std. No. Mp Mw/Mn Std. No. Mp Mw/MnS-20S-11S-6.9S-5.0S-4.4S-2.9S-1.9S-1.2S-1.0S-0.519,80010,7006,9305,0304,4302,9001,9301,2001,0505801.021.021.031.031.031.031.051.071.071.12S-3730S-2480S-1230S-579S-197S-55.1S-31.4S-12.8S-3.95S-1.20[Polymethylmethacrylate (PMMA)]Features3,730,0002,480,0001,230,000579,000197,00055,10031,40012,8003,9501,200F8604075 STANDARD M-75 0.5g x 7 kinds1.041.051.051.021.021.031.021.021.031.07S-7450S-3790S-3250S-2220S-1820S-1060S-5917,450,0003,790,0003,250,0002,220,0001,820,0001,060,000591,000For organic solvent SEC (GPC)Narrow molecular weight distribution rangeEasily soluble in hexafluoroisopropanol (HFIP) and dimethylformamide (DMF)Kit typeProduct Code[Pullulan]FeaturesProduct Name Contents MW Range1,800~950,000(Note)Molecular weights (Mp, Mw/Mn) of eachkit may vary depending on production lots.For aqueous SEC (GFC)Unbranched pullulan standardHigh solubility in water eliminates the possibility of recrystallization1.071.051.041.031.041.031.03(Note)Molecular weights (Mp, Mw/Mn)of each kit may vary dependingon production lots.Std. No. Mp Mw/MnM-949M-451M-139M-53M-21M-7.1M-1.9949,000451,000139,00052,60020,8007,1001,8901.051.021.051.021.031.081.1063Calibration Standards for SECKit typeProduct CodeF8400000 STANDARD P-82 0.2g x 8 kindsSingle typeProduct CodeF8402500F8401300F8400800F8400400F8400200F8400100F8400050F8400020F8400010F8400005F8400003F8400002F8400001Product NameProduct NameNEW STD P-2500NEW STD P-1300STD P-800STD P-400STD P-200STD P-100STD P-50STD P-20STD P-10STD P-5STD P-3STD P-2STD P-1Contents0.2g0.2g0.5g0.5g0.5g0.5g0.5g0.5g0.5g0.5g0.2g0.2g0.2gContentsMp2,350,0001,220,000708,000375,000200,000107,00047,10021,10011,1005,9002,8902,1501,420MW Range5,000~800,000Mw/Mn1.491.371.231.131.131.121.061.071.101.091.121.111.18Std. No.P-800P-400P-200P-100P-50P-20P-10P-5(Note)Molecular weights (Mp, Mw/Mn)of each kit may vary dependingon production lots.Structural formula of P seriesO CH 2 H HCH 2OHH HCH 2OHHHHOHOHOOHOHH O OH H O OH HH OHH OHH OHn
Page 1 and 2:
Capture the EssenceHPLC Columns and
Page 3 and 4:
ContentsColumn selectionSample pret
Page 5 and 6:
HPLC Separation ModesLiquid chromat
Page 7 and 8:
FoodsNutritionalingredientsFood saf
Page 9 and 10:
Preparation of Test Samples Used in
Page 11 and 12:
Systems diagram for column switchin
Page 13 and 14: Polymer-based Packed Columns for Re
Page 15 and 16: Polymer-based Packed columns forHyd
Page 17 and 18: Comparison between ODP2 HP and ODP-
Page 19 and 20: Hydantoins3214RONONHSample :Hydanto
Page 21 and 22: 0Saccharides analysis using corona-
Page 23 and 24: Hippuric acids in urineStandards123
Page 25 and 26: Medicinal chemicalssampleAlprenolol
Page 27 and 28: Protein separation using QA-8251234
Page 29 and 30: Protein separation using cation exc
Page 31 and 32: ApplicationProduct NameAFpak AAB-89
Page 33 and 34: Column Trouble ShootingCommon cause
Page 35 and 36: FeaturesDC-613,SC1211,SZ5532Separat
Page 37 and 38: 6 78Saccharides in wood12Sample : 5
Page 39 and 40: Maltooligosuccharides, organic acid
Page 41 and 42: Anion analysis with non-suppressor
Page 43 and 44: Standard cations (YS-50 and YK-421)
Page 45 and 46: Precautions for polar polymer analy
Page 47 and 48: Calibration curves for KW400 series
Page 49 and 50: Calibration curves for SB400 series
Page 51 and 52: Calibration curves for GS-HQ series
Page 53 and 54: Calibration curves for GF-HQ series
Page 55 and 56: Calibration curves for KF-800 serie
Page 57 and 58: Calibration curves for KD-800 serie
Page 59 and 60: Calibration curves for KF-600 serie
Page 61 and 62: Calibration curve for LF-804 usingP
Page 63: Calibration curves for HFIP-800 ser
Page 67 and 68: FoodsNutritional ingredientsColumn
Page 69 and 70: BiotechnologyColumn list for Biotec
Page 71 and 72: USP(United States Pharmacopeia) Col
Page 73 and 74: I.D.2.0mm1.0mm0.8mm0.5mm0.3mm2.0mm1
Page 75 and 76: AFpak AAB-894 semi-micro and micro
Page 77 and 78: AFpak ALC-894 semi-micro and micro
Page 79 and 80: KW402.5 semi-micro and micro typeBa
Page 81 and 82: I.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmL
Page 83 and 84: Asahipak GF-310 HQ semi-micro and m
Page 85 and 86: Preparative columnsPolymer-based Pa
Page 87 and 88: Columns for Ligand Exchange Chromat
Page 89 and 90: GPC K-800 series Preparative typePr
Page 91 and 92: General precautions for column hand
Page 93 and 94: Refractive Index DetectorShodex RI
Page 95 and 96: Instable baseline valuesSTARTColumn
Page 97 and 98: II-524A, IA-GJJJ-50KK-801~807, K-80
Page 99 and 100: Useful Information Regarding Shodex
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