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Preparative columnsPreparativeColumns84Columns for Anion Exchange Chromatography Columns for Cation Exchange ChromatographyPreparativeColumnsF6548000F6548020F6548050F6709602F6548001F6548021F6598051F6709603F6840004F6710021F6548002F6548022F6548052F6709604F6548003F6548023F6548053F6709605Preparative columnsIEC QA-825 Preparative typeProduct CodeProduct NameIEC QA-2025IEC QA-2825IEC QA-5025IEC QA-LGIEC DEAE-825 Preparative typeProduct CodeProduct NameIEC DEAE-2025IEC DEAE-2825IEC DEAE-5025IEC DEAE-LGAsahipak ES-502N Preparative typeProduct CodeProduct NameAsahipak ES-502N 20CAsahipak GS-20G 7BIEC SP-825 Preparative typeProduct CodeIEC SP-2025IEC SP-2825IEC SP-5025IEC SP-LGIEC CM-2025IEC CM-2825IEC CM-5025IEC CM-LGColumns for Anion Exchange ChromatographyFunctional GroupQuaternary ammoniumQuaternary ammoniumQuaternary ammonium(guard column)DiethylaminoethylDiethylaminoethylDiethylaminoethyl(guard column)Diethylaminoethyl(guard column)Particle Size(μm)20373720Particle Size(μm)20373720Column Size (mm)I.D. x L20.0 × 15028.0 × 30050.0 × 3008.0 × 50Column Size (mm)I.D. x L20.0 × 15028.0 × 30050.0 × 3008.0 × 50Columns for Cation Exchange ChromatographyProduct NameIEC CM-825 Preparative typeProduct CodeProduct NameFunctional GroupFunctional GroupFunctional GroupSulfopropylSulfopropylSulfopropyl(guard column)Functional GroupCarboxymethylCarboxymethylCarboxymethyl(guard column)Base Material : PolyhydroxymethacrylateBase Material : PolyhydroxymethacrylateParticle Size(μm)1320Base Material : Polyvinyl alcoholParticle Size(μm)2020.0 × 1503728.0 × 3003750.0 × 300208.0 × 50Base Material : PolyhydroxymethacrylateParticle Size(μm)20373720Column Size (mm)I.D. x L20.0 × 1007.5 × 50Column Size (mm)I.D. x LColumn Size (mm)I.D. x L20.0 × 15028.0 × 30050.0 × 3008.0 × 50Base Material : Polyhydroxymethacrylate* See page 24 for IEC QA-825.* See page 71 for semi-microcolumns and micro columns.* See page 24 for IEC DEAE-825.* See page 71 for semi-microcolumns and micro columns.* See page 24 for Asahipak ES-502N.* See page 71 for semi-microcolumns and micro columns.* See page 26 for IEC SP-825.* See page 72 for semi-microcolumns and micro columns.* See page 26 for IEC CM-825.* See page 72 for semi-microcolumns and micro columns.Asahipak ES-502C Preparative typeProduct Code Product NameFunctional GroupF6840003 Asahipak ES-502C 20C CarboxymethylF6710021 Asahipak GS-20G 7B (guard column)Particle Size(μm)1320Column Size (mm)I.D. x L20.0 × 1007.5 × 50Base Material : Polyvinyl alcohol* See page 26 for Asahipak ES-502C.* See page 72 for semi-microcolumns and micro columns.*Contact Shodex or our distributors near you for larger preparative columns.
Columns for Ligand Exchange ChromatographySUGAR KS-800 series Preparative typeProduct Code Product NamePlate Number(TP/column)Counter IonParticle Size(μm)Column Size (mm)I.D. x LStandard ColumnF6502007F6502008F6502009F6502010F6502011F6502012F6700002SUGAR KS-2001SUGAR KS-2002SUGAR KS-2003SUGAR KS-2004SUGAR KS-2005SUGAR KS-2006SUGAR KS-LG≥ 7,000≥ 7,000≥ 8,000≥ 6,000≥ 6,000≥ 6,000(guard column)Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )1313131818181320.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 3008.0 × 50KS-801KS-802KS-803KS-804KS-805KS-806(guard column)* See page 32 for SUGAR KS-800.Base Material : Styrene divinylbenzene copolymerRSpak DC-613 Preparative typeProduct CodeF6514013 RSpak DC-2013F6700402 RSpak DC-LGF6514021 RSpak DC-5013F6700172 RSpak DC-LLG* See page 33 for RSpak DC-613.F6505012 RSpak KC-2011F6700010 RSpak KC-LG* See page 36 for RSpak KC-811.Plate Number(TP/column)≥ 6,000(guard column)−(guard column)Columns for Ion-exclusion ChromatographyPlate Number(TP/column)≥ 8,000(guard column)PROTEIN KW-800 series Preparative typeProduct Code Product NamePlate Number(TP/column)F6505020F6505021F6505022F6709556PROTEIN KW-2002.5PROTEIN KW-2003PROTEIN KW-2004PROTEIN KW-LG≥ 17,000≥ 17,000≥ 14,000(guard column)* See page 44 for PROTEIN KW-800.* See page 77 for semi-micro columns and micro columns.F6516011F6516012F6516013F6516014F6516015F6516016F6516017F6709555Product NameRSpak KC-811 Preparative typeProduct CodeProduct NameOHpak SB-2002OHpak SB-2002.5OHpak SB-2003OHpak SB-2004OHpak SB-2005OHpak SB-2006OHpak SB-2006MOHpak SB-LG* See page 46 for OHpak SB-800 HQ.* See page 78, 79 for semi-micro columns and micro columns.Aqueous SEC(GFC) columns : Silica-basedAqueous SEC(GFC) columns : Polymer-basedOHpak SB-800 HQ series Preparative typeProduct CodeProduct NamePlate Number(TP/column)≥ 9,000≥ 12,000≥ 12,000≥ 12,000≥ 12,000≥ 12,000≥ 12,000(guard column)Counter IonSulfo(Na + )Sulfo(Na + )Sulfo(Na + )Sulfo(Na + )1010101020.0 × 3008.0 × 5050.0 × 30020.0 × 100Base Material : Styrene divinylbenzene copolymerFunctional GroupSulfoSulfoPreparative columnsParticle Size(μm)Particle Size(μm)1313Particle Size(μm)5577Particle Size(μm)1510101820201318Column Size (mm)I.D. x LColumn Size (mm)I.D. x L20.0 × 3008.0 × 50Base Material : Styrene divinylbenzene copolymerColumn Size (mm)I.D. x L20.0 × 30020.0 × 30020.0 × 3008.0 × 50Column Size (mm)I.D. x L20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 3008.0 × 50PreparativeColumnsStandard ColumnKW-802.5KW-803KW-804(guard column)Base Material : SilicaStandard ColumnSB-802 HQSB-802.5 HQSB-803 HQSB-804 HQSB-805 HQSB-806 HQSB-806M HQ(guard column)Base Material : PolyhydroxymethacrylatePreparative columnsPreparativeColumns85Columns for Ligand Exchange Chromatography Columns for Ion-exclusion ChromatographyAqueous SEC(GFC) columns : Silica-based Aqueous SEC(GFC) columns : Polymer-based(Note) The maximum solvent tolerance of the SB-2000 series for methanol, acetonitrile, and DMF is 50%.Solvent tolerance differs from that of the standard SB-800 HQ columns.*Contact Shodex or our distributors near you for larger preparative columns.
Page 1 and 2:
Capture the EssenceHPLC Columns and
Page 3 and 4:
ContentsColumn selectionSample pret
Page 5 and 6:
HPLC Separation ModesLiquid chromat
Page 7 and 8:
FoodsNutritionalingredientsFood saf
Page 9 and 10:
Preparation of Test Samples Used in
Page 11 and 12:
Systems diagram for column switchin
Page 13 and 14:
Polymer-based Packed Columns for Re
Page 15 and 16:
Polymer-based Packed columns forHyd
Page 17 and 18:
Comparison between ODP2 HP and ODP-
Page 19 and 20:
Hydantoins3214RONONHSample :Hydanto
Page 21 and 22:
0Saccharides analysis using corona-
Page 23 and 24:
Hippuric acids in urineStandards123
Page 25 and 26:
Medicinal chemicalssampleAlprenolol
Page 27 and 28:
Protein separation using QA-8251234
Page 29 and 30:
Protein separation using cation exc
Page 31 and 32:
ApplicationProduct NameAFpak AAB-89
Page 33 and 34:
Column Trouble ShootingCommon cause
Page 35 and 36: FeaturesDC-613,SC1211,SZ5532Separat
Page 37 and 38: 6 78Saccharides in wood12Sample : 5
Page 39 and 40: Maltooligosuccharides, organic acid
Page 41 and 42: Anion analysis with non-suppressor
Page 43 and 44: Standard cations (YS-50 and YK-421)
Page 45 and 46: Precautions for polar polymer analy
Page 47 and 48: Calibration curves for KW400 series
Page 49 and 50: Calibration curves for SB400 series
Page 51 and 52: Calibration curves for GS-HQ series
Page 53 and 54: Calibration curves for GF-HQ series
Page 55 and 56: Calibration curves for KF-800 serie
Page 57 and 58: Calibration curves for KD-800 serie
Page 59 and 60: Calibration curves for KF-600 serie
Page 61 and 62: Calibration curve for LF-804 usingP
Page 63 and 64: Calibration curves for HFIP-800 ser
Page 65 and 66: Calibration Standards for SEC[Polys
Page 67 and 68: FoodsNutritional ingredientsColumn
Page 69 and 70: BiotechnologyColumn list for Biotec
Page 71 and 72: USP(United States Pharmacopeia) Col
Page 73 and 74: I.D.2.0mm1.0mm0.8mm0.5mm0.3mm2.0mm1
Page 75 and 76: AFpak AAB-894 semi-micro and micro
Page 77 and 78: AFpak ALC-894 semi-micro and micro
Page 79 and 80: KW402.5 semi-micro and micro typeBa
Page 81 and 82: I.D.4.6mm2.0mm1.0mm0.8mm0.5mm0.3mmL
Page 83 and 84: Asahipak GF-310 HQ semi-micro and m
Page 85: Preparative columnsPolymer-based Pa
Page 89 and 90: GPC K-800 series Preparative typePr
Page 91 and 92: General precautions for column hand
Page 93 and 94: Refractive Index DetectorShodex RI
Page 95 and 96: Instable baseline valuesSTARTColumn
Page 97 and 98: II-524A, IA-GJJJ-50KK-801~807, K-80
Page 99 and 100: Useful Information Regarding Shodex
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