Asahipak - Hplc.eu

Column Trouble ShootingCommon causes for abnormal chromatogramsInformationUnstable baseline values······Main cause······Solution······Frequent problemPeak tailingSelection ofcolumn and/or eluentappropriateNOYESStrong sample retentionVoid existing at top of columnChange column and/or eluentReplace columnPeak split,shouldersAdsorption of impurities incolumnChanneling in column· Wash column· Check column performance· Replace columnReplace column31PeakleadingBroad peakGhost peakProblem solvedby reducinginjection volumeNOYESLeading caused by sampleproperties(ionic and micellar properties)· Adsorption of impurities incolumn· Deterioration of columnSample overloadContaminationImpurities existing in eluentChange column and/or eluent· Wash column· Check column performance· Replace columnReduce injection volumePrepare sample againWash flow line of HPLCReplace eluentColumn Trouble ShootingNo peakSystem trouble· Eluent not flowing· Injector at wrong position (load/inject)· Detector deactivatedCheck systemSample adsorption in columnRepeat sample injection· Column equilibration insufficientPressure elevationPumpA B CColumn Detector······Main causeSTARTInjector······Solution······Frequent problemSalt precipitated isobserved in eluentYESPressure still highafter washingNOSalting out· Change eluent composition· Wash system before analysisNOYESPressure still high whenC is disconnectedNODetector pipingblocked· Clean up detector· Replace pipingYESPressure still high whenB is disconnectedYESNOColumnblockedPressure undermaximum columnpressureYESNOScreen eluent and samplethrough 0.45m filterYESNODeteriorationof columnColumn end-fittingblocked· Check column performance· Replace columnReplace columnPressure still high whenA is disconnectedNOInjector blocked· Clean up injector· Replace injectorYESUsed line filterYESNOPump piping blocked· Replace filter in pump· Clean up pump· Replace pump pipingLine filter blocked· Wash line filter· Replace line filter