Asahipak - Hplc.eu

0Saccharides analysis using corona-charged aerosol detector0 5112Silica-type amide column(4.6mmI.D. x 150nm)121Silica-type amino column(4.6mmI.D. x 250nm)5 10 15 min23Sample : 40μg/mL each, 5μL1. Fructose2. Glucose4526NH2P-50 4E(4.6mmI.D. x 250nm)Column : Shodex Asahipak NH2P-50 4ESilica based amino column (company-A),Silica based amide column (company-B)Eluent : H 2 O/CH 3 CN/=20/80Flow rate : 1.0mL/minDetector : Corona charged aerosolColumn temp : 30˚C(Amino column), 80˚C(Amide column)Fructooligosaccharide syrupSample :Fructooligosaccharide syrup, 2.5%, 20μL1. Fructose2. Glucose3. Sucrose4. 1-Kestose5. Nystose6. 1-Fructofuranosyl-D-nystose10 15 20 25 minColumn : Shodex Asahipak NH2P-50 4EEluent : CH 3 CN/H 2 O=70/30Flow rate : 1.0mL/minDetector : RIColumn temp. : 25˚CLC/TOF-MS analysis of 2-amino benzoic acid-derivatizedsugar chains123A corona-charged aerosoldetector measures effluentsas particles. Accordingly, thebaseline will be significantlyinfluenced by componentseluted from the column. Thepolymer-based amino column,NH2P, elutes extremely smallamounts of components, andthus enables a stable baselinewith lower noise level to beestablished.Oligogalacturonic acids1234Sample : Oligogalacturonic acids1. Galacturonic acid2. Oligogalacturonic acid dimer3. Oligogalacturonic acid trimer4. Oligogalacturonic acid tetramer5. Oligogalacturonic acid pentamer6. Oligogalacturonic acid hexamer56DP 1 2 3 4 5 6 70 1 2 3 4 5 6 7 8 9 10Retention time (min)ChitooligosaccharidesSample : Chitooligosaccharides 2%, 20μL1. D-Glucosamine2. Chitobiose hydrochloride3. Chitotriose hydrochloride4. Chitotetraose hydrochloride5. Chitopentaose hydrochloride6. Chitohexaose hydrochloride0 5 10 15 20 min 0 5 10 15 minColumn : Shodex Asahipak NH2P-50 4EEluent : 0.3M Sodium phosphate buffer(pH4.4)Flow rate : 1.0mL/minDetector : UV(210nm)Column temp. : 40˚CSample offered by Prof. Yoshino in Kyoto Women's Univ.Sample : 2μLHuman IgG (1μg protein)2AA-labeled N-Glycan* 2AA : 2-aminobenzoic acidProducts for cellulose degradation by cellulase (PcCel45A)treatmentColumn : Shodex Asahipak NH2P-50 4EEluent : (A);H 2 O/CH 3 CN=40/60(B);H 2 O/CH 3 CN=50/50Linear gradient; (A) to (B),10minFlow rate : 1.0mL/minDetector : Corona charged aerosolColumn temp : 40˚CData provided by Kiyohiko Igarashi, Professor of the Laboratory of Forest Chemistry,Biomass Chemistry Course, Department of Biomaterial Sciences,Graduate School of Agricultural and Life Sciences, University of TokyoPyridylaminated monosaccharides123123Column : Shodex Asahipak NH2P-50 4EEluent : CH 3 CN/H 2 O=70/30Flow rate : 1.0mL/minDetector : RIColumn temp. : 25˚C4 5 6544Sample : 5μL1. 2-Aminopyridine 0.1pmol/μL2. PA-Rhamnose 1pmol/μL3. PA-Fucose 1pmol/μL4. PA-Ribose 1pmol/μL5. PA-Xylose 1pmol/μL6. PA-N-Acetylglucosamine 1pmol/μL7. PA-N-Acetylgalactosamine 1pmol/μL8. PA-Mannose 1pmol/μL9. PA-Glucose 1pmol/μL10. PA-Galactose 1pmol/μL56Standard analysiscolumns19Polymer-based Packed columns for Hydrophilic Interaction Chromatography (HILIC)6789105 10Migration time (min)15 20Column : Shodex NH2P40-2DEluent : A; 95% CH 3 CN/0.1% Formic acidB; 5% CH 3 CN/0.1% Formic acidGradient : Linear gradient: B%, 30%(0-2.5min), 30-95%(2.5-20min)Flow rate : 0.2mL/minDetector : ESI-TOF MS(Polarity : Negative, Full MS range : 2000 )Column oven : 45˚CData provided by Mr. Michihiro Kinoshita, Faculty of Pharmacy, Kinki University020 40 60 minColumn : Shodex Asahipak NH2P-50 4EEluent : H 3PO 4/H 2O/CH 3CN=1/14/85Flow rate : 0.5mL/minDetector : Fluorescence(Ex. : 310nm, Em. : 380nm)Column temp. : 40˚C