Asahipak - Hplc.eu

FeaturesDC-613,SC1211,SZ5532Separation is performed by combining ligand exchange and HILIC modesDC-613 can analyze sugars without removing sodium salts in the sampleSC1211 is suitable for separation of sugar alcoholsSZ5532 is recommended for the separation of disaccharides or trisaccharides[DC-613]Standard analysiscolumnsNotebookNo.2PreparativeColumnsp.85Standard columnsLigand exchange and HILICProduct CodeF7001003F6700170F7001400F6700120F7001300F6700110SubstabcesArabinoseD-ArabitolDulcitolmeso-ErythritolProduct NameRSpak DC-613RSpak DC-GSUGAR SC1211SUGAR SC-GSUGAR SZ5532SUGAR SZ-G≥ 5,500(guard column)≥ 5,500(guard column)≥ 5,500(guard column)SP0810 SC1011 KS-801 SZ553210.4215.8620.1812.708.9111.3312.7610.098.217.637.407.86Plate Number(TP/column)Elution Volume (mL)5.117.279.465.73NH2P-504E6.186.297.455.43Functional group(Counter Ion)Sulfo(Na + )Sulfo(Na + )Sulfo(Ca 2 + )Sulfo(Ca 2 + )Sulfo(Zn 2 + )Sulfo(Zn 2 + )SC1211 DC-6135.568.1611.286.276.036.067.814.95Particle Size(μm)Elution volume of saccharides analysis with various columns[Partial list only; refer to website for complete list]61061066SubstabcesD-MannoseMelibioseNystosePalatinitPore Size(Å)100−50−100−Column Size(mm)I.D. x L6.0 × 1504.6 × 106.0 × 2504.6 × 106.0 × 1504.6 × 10H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=75/25H 2 O/CH 3 CN=75/25H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=30/70Base Material : Styrene divinylbenzene copolymerSP0810 SC1011 KS-801 SZ553210.728.166.382peaks8.176.455.452peaksElution Volume (mL)7.645.984.935.905.8311.6920.052peaksNH2P-504EShipping SolventSC1211 DC-6137.84 5.0114.70 4.2331.90 *12.73 2peaks7.1516.39—2peaks33Columns for Ligand Exchange ChromatographyD(-)-Fructose11.058.857.715.376.755.906.52Palatinose7.846.455.898.0812.123.9910.56D(+)-Fucose10.488.848.094.505.434.965.04Panose7.145.785.3216.8725.60*25.89D(+)-Galactose9.747.987.586.468.104.987.91D(+)-Raffinose7.145.785.2916.3620.25*21.60Gentiobiose7.226.085.7510.5016.36*14.45Rhamnose9.778.237.373.935.524.434.23Glucose8.637.307.175.878.614.767.35D(-)-Ribose19.3513.669.044.825.458.645.55myo-Inositol12.778.867.9912.639.967.8717.36D(-)-Sorbitol21.6113.317.429.797.0911.887.68Isomaltose7.686.265.9510.5715.18*15.24Sorbose9.678.037.385.127.354.926.15Isomaltotriose7.095.755.3421.1727.55*37.45Stachyose6.825.574.97—36.22*—1-Kestose6.795.755.2613.0920.11*11.11Sucrose7.546.295.877.9111.87*9.45Kojibiose7.566.215.889.6514.82*12.70α-D-Talose21.3312.598.765.696.478.516.86Lactitol13.278.096.1316.3511.826.6715.26Trehalose7.626.275.7810.8513.25*12.42Lactose8.056.515.9910.1213.274.0713.04Trehalulose8.926.956.109.5411.684.7812.41Lactulose9.136.996.199.1610.724.6511.88Xylitol19.8713.147.947.776.1010.166.44Maltitol12.238.266.0313.0411.826.7713.12Xylobiose8.166.686.405.659.05*7.04Maltose7.856.345.948.6714.24*11.46D(+)-Xylose9.217.907.714.556.584.485.35Maltotriose7.485.895.3813.7924.96*19.92D-Xylulose10.649.028.044.065.415.074.65Mannitol15.8011.107.238.757.399.037.22(—) Not detected ( * ) Overlap with solvent peak(—) Not detected ( * ) Overlap with solvent peakColumn : SUGAR SP0810, SC1011, KS-801Eluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : 80˚CColumn : SUGAR SC1211Eluent : H 2O/CH 3CN=65/35Flow rate : 1.0mL/minDetector : RIColumn temp. : 70˚CColumn : SUGAR SZ5532Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 60˚CColumn : Asahipak NH2P-50 4EEluent : H 2O/CH 3CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 30˚CColumn : RSpak DC-613Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 70˚C