Asahipak - Hplc.eu

Instable baseline valuesSTARTColumn PressurenormalYESBaseline stable whenpump stoppedYESLeakage observedYESFlow rate fluctuationHPLC System Trouble ShootingNONONOCommon cause of abnormal chromatogramsYESMonotonic driftNO······Main cause······SolutionMalfunction of check valveTemperature fluctuationDeterioration of detector lampInsufficient groundingMalfunction of recorderBubbles in flow cellContamination of flow cellInsufficient eluent replacement(with different solvent, salt or others)Contamination of columnPulsation of pump······Frequent problem· Replace check valve· Clean up check valve· Warm up detector sufficiently· Shield detector from wind· Check column oven· Check detector temperature controlReplace lampEstablish sufficient groundingRepair recorderWash inside of detectorWash inside of detectorWash flow line of HPLC· Wash column· Check column performance· Replace columnUse damperInformation HPLC System Trouble Shooting93Tighten connectionsDeterioration of eluent· Use eluent containing stabilizer(for THF, use THF containing BHT)Noise······Main cause······Solution······Frequent problemSTARTInsufficient groundingEstablish sufficient groundingBaseline stable whenpump stoppedNOIrregular noiseDeterioration of detector lampReplace detector lampYESMalfunction of recorderRepair recorderShodex DEGAS usedNOInsufficient degassing eluentUse Shodex DEGASYESSpike noiseYESOKSpike noise foundfrom beginingNOYESAir in columnProblem solved by applying backpressure to column(*)(*) Do not apply back pressure to detectorColumn temperature exceeding eluent boiling pointNO· Check column performance· Replace columnReduce temperature of columnoven and/or post-column reactioncoilNoise shape leadingNOContamination of flow cellWash flow cellYESBubbles in flow cellRepair detector