Asahipak - Hplc.eu

Comparison between ODP2 HP and ODP-50 (existing)ODP2 HP-4D123N:11,4004ODP-50 4D (existing)1Sample : 5μL1. Phenol 300mg/L2. Methyl benzoate 350mg/L3. Toluene 1000mg/L4. Naphthalene 150mg/L23N:5,7004Imidazoles12Sample : 0.1% each, 10μL1. Imidazole2. 2-Methylimidazole3. 4-Methylimidazole3Standard analysiscolumns0 5 10min0 5 10 0510minminColumn : Shodex ODP2 HP-4DEluent : H 2 O/CH 3 CN=55/45Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 40˚CIntensityIntensity120000110000100000900008000070000600005000040000300002000010000012000011000010000090000800007000060000500004000030000200001000001000000ODP2 HPBarbital 500ng/mLODS 1st8000001100001000006000009000080000700004000006000050000Barbital40000Barbital200000Peak Area : 100%30000Peak Area : 100%200000100000BarbitalPeak Area : 99%0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5Time (min)IntensityIntensityColumn : Shodex AsahipakODP-50 4DEluent : H 2 O/CH 3 CN=35/65Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 40˚CComparison of recovery rates of the barbital in BSA betweenODP2 HP-2B and ODSFor the LC/MS analysis of chemical substances containing proteins or other similarsamples, it is generally said that an ODS column is susceptible to ion suppressiondue to the presence of proteins. However, ODP2 HP does not hold proteins andelutes them at one time, and thus is resistant to the effects of such ion suppression.Barbital 500ng/mLBarbital 500ng/mL in BSA 7.0mg/mLBarbital 500ng/mL in BSA 7.0mg/mL1100001000009000080000700006000050000400003000020000100000Column : Shodex ODP2 HP-2B, ODS (company-A)Eluent : 10mM Ammonium acetate aq./CH 3 CN=70/30Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative : m/z 183)Column temp. : 30˚CInjection vol. : 10μLBarbitalPeak Area : 71%0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5Time (min)Column : Shodex ODP2 HP-4EEluent : 10mM Na 2 HPO 4 aq./CH 3 CN=90/10Flow rate : 0.8mL/minDetector : UV(220nm)Column temp. : 40˚CIntensityIntensity1000000Direct analysis of chemical substances in biofluids without pretreatmentRepeated results of LC/MS analysisAn experiment repeated 150 times for chemical substance analysis in the presenceof protein (BSA) revealed that ODP2 HP elutes protein without retaining it and henceposes no significant problem in terms of repeatability.800000600000400000200000150th00 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18Time (min)Sample :500ng/mL each in 0.7mg/mL BSA, 5μL1. Lidocaine2. ImipramineColumn : Shodex ODP2 HP-2BEluent : 10mM Ammonium acetate aq./CH 3 CN=70/30Flow rate : 0.2mL/minDetector : ESI-MSColumn temp. : 40˚C[Direct analysis of serum acetaminophen] [Direct analysis of serum dihydrocodeine phosphate] [Direct analysis of risperidone in urine]15Polymer-based Packed Columns for Reversed Phase ChromatographyAcetaminophen(20.7μg/mL)21.10minSample :Human serum, 10μLAcetaminophen2-Acetaminophenol2-Acetaminophenol (internal standard)25.2minSample : Human serum, 10μLDihydrocodeine phosphate(74.6μg/mL)29.9minSample : 10μLHuman Urine (5 fold diluted)RisperidoneRisperidone(6.4μg/mL)38.0min0 10 20 30 40 min0 10 20 30 min 0 10 20 30 40 minColumn : Shodex ODP2 HP-4DEluent : 0.1% TFA aq./CH 3 CN=93/7Flow rate : 0.5mL/minDetector : UV(254nm)Column temp. : 40˚CColumn : Shodex ODP2 HP-4DEluent : 0.1% TFA aq./CH 3 CN=93/7Flow rate : 0.3mL/minDetector : UV(230nm)Column temp. : 40˚CColumn : Shodex ODP2 HP-4DEluent : 0.1% TFA aq./CH 3 CN=93/7Flow rate : 0.6mL/minDetector : UV(215nm)Column temp. : 40˚CData provided by Dr. Katsuko Hara and Dr. Yutaka Komiyama of the Department of Clinical Laboratory, Kansai Medical University Takii Hospital