Asahipak - Hplc.eu

6 78Saccharides in wood12Sample : 5μL1. Cellobiose 1.0%2. Glucose 1.5%3. Xylose 0.5%4. Galactose 0.5%5. Arabinose 0.5%6. Mannose 1.0%Maltose and isomaltose12Sample : 15μL1. Glucose 1.0%2. Maltose 1.0%3. Isomaltose 0.6%4. Maltotriose 1.0%Standard saccharides1Sample : 2.5% each, 15μL1. Xylose2. Fructose3. Glucose4. Galactose5. Sucrose6. Maltose7. Lactose8. Melibiose9. Maltotriose10. RaffinoseStandard analysiscolumns642 343 4 5359105 10 1520 min 0 24 6 8 10 12 min0 10 20 30 minColumn : Shodex SUGAR SP0810Eluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 85˚CSaccharides in the presence of sodium saltThe RSpak DC-613 enables the analysis of saccharides in sodium-salt containingsample without desalting because the column uses Na as counter ion.Hydrolyzed samples with acids such as hydrochloric acid and sulfuric acid can beanalyzed as sample solution after being neutralized with sodium hydroxide.Solvent for samplepreparation1% NaCl aq./CH 3 CN=50/501% Na 2 SO 4 aq./CH 3 CN=50/50H 2 O/CH 3 CN=50/500 51min23Sample : 0.1% each, 10μL1. Xylose2. Sucrose3. Cellobiose4. Lactose4Column : Shodex SUGAR SZ5532Eluent : H 2 O/CH 3 CN=25/75Flow rate : 0.9mL/minDetector : RIColumn temp. : 60˚C10 15Pinitol05123Columnn : Shodex RSpak DC-613Eluent : H 2 O/CH 3 CN=20/80Flow rate : 1.5mL/minDetector : RIColumn temp. : 70˚C4Sample : 0.1% each, 10μL1. Pinitol2. Fructose3. Glucose4. Sucrose5. chiro-Inositol6. myo-Inositol5 610 1520 min35Columns for Ligand Exchange ChromatographyColumn : Shodex RSpak DC-613Eluent : H 2 O/CH 3 CN=30/70Flow rate : 0.8mL/minDetector : RIColumn temp. : 50˚C* High concentration of acetonitlie isapplied to eluent of saccharideanalysis using DC-613. Take care ofsalt concentration in sample to avoidsalt deposition from sample solution.Column : Shodex RSpak DC-613Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 70˚CMoisturizing componentsMannitol analysis with USP methodSample : 0.1% each, 20μL1. 1,3-Propanediol2. Propylene glycol3. Ethylene glycol4. Glycerin5. meso-ErythritolAccording to the USP (United States Pharmacopeia) method, mannitol should beanalyzed using a column which can separate mannitol and sorbitol with a degreeof separation equal to or greater than 2.0. The USPpak MN-431 is a columnspecially designed for mannitol analysis, which satisfies this criteria.1Sample : 10μL1. Mannitol 0.5%2. Sorbitol 0.5%2241350 51015 min0 24 6 8 minColumn : Shodex SUGAR SC1211Eluent : H 2 O/CH 3 CN=60/40Flow rate : 0.6mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex USPpak MN-431Eluent : H 2 OFlow rate : 0.5mL/minDetector : RIColumn temp. : 60˚C