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Standard cations (YS-50 and YK-421)YS-50 10μL inj.5μS/cm3241565μS/cmYK-42112315μL inj.46Sample :1. Li + 2mg/L2. Na + 10mg/L3. NH + 4 10mg/L4. K + 20mg/L5. Mg 2+ 10mg/L6. Ca 2+ 20mg/L5YS-50 is a high-performance columnwith a theoretical plate number whichis approximately twice as large asthat of YK-421. In particular, the peakshape and quantitative performancefor divalent ions have been improved.The quantitative performance forNH 4+ in a system containing Na + athigh concentration has beenenhanced as wellResolutionfactor ofNa + and NH 4+YS-502.3YK-4211.8Standard analysiscolumns05 10 15 20 min0 5 10 15 minTPMg 2+Ca 2+YS-50YK-4217,000 3,2006,900 3,300Column : Shodex IC YS-50Eluent : 4mM Methanesulfonic acid aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CEffect of adding crown ether to eluentA crown ether molecule forms complex with cations.Sample : 10μLThe elution of cations (in particular, K + ) can be controlled by the 1. Li + 2mg/Ldifference in the ability of complex formation2. Na + 10mg/L3. NH4 + 10mg/L4. K + 20mg/L1 23 5. Mg 2+ 10mg/L236. Ca 2+ 20mg/L14 56518-Crown 6-ether conc.6mM3mM1mM0mM4440 5 10 15 20 minColumn : Shodex IC YS-50Eluent : 4mM Methanesulfonic acid + 18-Crown 6-ether aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C555666Column : Shodex IC YK-421Eluent : 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CSimultaneous analysis for cations and ethylenediamine46Sample : 10μL1. Li + 2mg/L2. Na + 10mg/L3. NH4 + 10mg/L4. K + 20mg/L5. Mg 2+ 10mg/L6. Ca 2+ 20mg/L7. Ethylenediamine 50mg/L0 5 10 15 20 25 minColumn : Shodex IC YS-50Eluent : 4mM Nitric acid + 1.5mM 18-Crown 6-ether aq./CH 3CN=90/10Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C741Columns for Ion Chromatography (Cation analysis)Alkylamines Alkaline earth metal ions Transition metal ions in red wine1231 2 3 4 5 6 7 8 9 10 min5 62Sample : 50μL1. NH4 + 5mg/L2. Methylamine 5mg/L3. Dimethylamine 5mg/L4. Trimethylamine 10mg/L5. Ethylamine 10mg/L6. Propylamine 10mg/L7. Butylamine 10mg/L1 2 3 4 5 6 7 8 9 10 minColumn : Shodex IC YK-421Eluent : 4mM Phosphoric acid aq. + 10% CH 3CNFlow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 25˚C74min0246231Cu 2+Zn 2+Ni 2+Co 2+Mg 2+Mn 2+ Fe 2+Cd 2+Pb 2+Ca 2+Sr 2+0 2 4 6 8 10 minSample : 100μL1. Mg 2+ 20mg/L2. Ca 2+ 50mg/L3. Sr 2+ 100mg/L4. Ba 2+ 200mg/LColumn : Shodex IC Y-521Eluent : 4mM Tartaric acid + 2mM Ethylenediamine aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C124 534Sample : Red wine(diluted 2-fold followed byaddition of 40mL HCl), 70μL1. Fe 3+2. Zn 2+3. Co 2+4. Fe 2+5. Mn 2+0 4 8 12 16 20 24 28 minColumn : Shodex IC T-521Eluent : 6mM Oxalic acid + 3mM Citric acid(pH4.1 adjusted by KOH)+ 0.1mM NaN 3 + 0.1mM NaHSO 3 aq.Reagent : 0.2mM *PAR + 3M NH 4 OH+ 1M Acetic acid aq.[Mixing coil(0.5mm I.D. x 1m)]Flow rate : 1.0mL/minDetector : VIS(530nm)Column temp. : 40˚C*PAR : 4-(2-Pyridylazo)resorcinol