Asahipak - Hplc.eu

Hippuric acids in urineStandards123Sample : 10μL1. Mandelic acid 17μg/mL2. Hippuric acid 17μg/mL3. o-Methylhippuric acid 17μg/mL4. p-Methylhippuric acid 17μg/mL5. m-Methylhippuric acid 17μg/mL6. Creatinine 33μg/mL4562Urine(10-fold diluted)6AflatoxinsReference : Shoku-An No. 0728001 (July 28, 2006, Japan)Test method of Aflatoxin B1 in cereals, puises, nuts andseeds, spices "multifunctional column"1234TFA methodSample :5μg/L each, 20μL1. Derived Aflatoxin G12. Derived Aflatoxin B 13. Aflatoxin G24. Aflatoxin B 2Standard analysiscolumns00510 min05Column : Shodex ODSpak F-411Eluent : [20mM Phosphoric acid + 20mM β-Cyclodextin(pH2.5)]/CH 3 CN=88/12+ 4.5mM Sodium dodecyl sulfateFlow rate : 1.0mL/minDetector : UV(225nm)Column temp. : 50˚CTrichothecene mycotoxinReference : Shoku-An No. 0717001 (July 17, 2003, Japan)Test method of deoxynivalenol1Sample : 20μL1. Nivalenol (INIV)2. Deoxynivalenol (DON)5 10 15 minColumn : Shodex Silica C18M 4EEluent : H 2 O/CH 3 CN/CH 3 OH=90/5/5Flow rate : 1.0mL/minDetector : UV(220nm)Column temp. : 40˚CBenzylpyridine isomers1* 1 ppm each of DON andNIV were added to wheatsample22Sample :1. 2-Benzylpyridine2. 3-Benzylpyridine3. 4-Benzylpyridine3Isoflavones12132310 min0 5 10 15 minDinitronaphthalene isomers4Sample :1. Daidzin 1.0μg2. Genistin 0.5μg3. Daidzein 1.0μg4. Genistein 1.0μgColumn : Shodex Silica C18P 4DEluent : 0.1% Acetic acid aq./CH 3 OH=70/30Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 30˚CSample :1. Naphtalene2. 1,5-Dimethylnaphtalene3. 1,5-Dinitronaphtalene4. 1,8-Dinitronaphtalene40 5 10 15 20 minColumn : Shodex Silica C18M 4EEluent : H 2 O/CH 3 CN/CH 3 OH/=60/10/30Flow rate : 1.0mL/minDetector : Fluorescence(Ex. : 365nm, Em. : 450nm)Column temp. : 40˚CCatechins in green tea121230 10 20 30 40 minColumn : Shodex Silica C18P 4DEluent : 0.1% Phosphoric acid aq./CH 3 OH=20/80Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 30˚CVitamin E34Sample :1. (-)-Epigallocatechin 1.0μg2. (-)-Catechin 1.0μg3 (-)-Epigallocatechin gallate 0.5μg4. (-)-Catechin gallate 1.0μg564Sample : 20μL1. α-Tocopherol 5μg/mL2. α-Tocotrienol 10μg/mL3. β-Tocopherol 5μg/mL4. γ-Tocopherol 5μg/mL5. γ-Tocotrienol 10μg/mL6. δ-Tocopherol 5μg/mL7. δ-Tocotrienol 10μg/mL721Silica-based Packed Columns for Reversed Phase, HILIC and Normal Phase Chromatography0 5 10 15 20 25 0 5 10 15 051015min min minColumn : Shodex Silica 5SIL 4DColumn : Shodex Silica 5PYE 4DColumn : Shodex Silica 5NPE 4DEluent : n-Hexane/Isopropanol/Acetic acidEluent : 20mM KH 2 PO 4 aq./CH 3 OH=40/60Eluent : H 2 O/CH 3 OH=30/70=1000/6/5Flow rate : 1.0mL/minFlow rate : 1.0mL/minFlow rate : 1.0mL/minDetector : UV(254nm)Detector : UV(254nm)Detector : Fluorescence(Ex. 298nm, Em. 325nm)Column temp. : 30˚CColumn temp. : 30˚CColumn temp. : 30˚C