Asahipak - Hplc.eu

Calibration curves for SB400 series and SB-800 HQ series using pullulan (H 2 O)Molecular weight (Pullulan)10 610 510 410 3SB403-4ESB402.5-4ESB401-4ESB404-4EMolecular weight (Pullulan)10 610 510 410 310 2SB-802 HQSB-806 HQSB-805 HQSB-803 HQSB-802.5 HQSB-804 HQSB-806M HQCalibration curves for SB-800 HQ series usingPEG and PEO (DMF)Molecular weight (PEG/PEO)10 610 510 410 3SB-803 HQSB-804 HQSB-802.5 HQSB-805 HQSB-806M HQSB-806 HQStandard analysiscolumns10 7 Elution volume (mL)10 7 Elution volume (mL)10 7 Elution volume (mL)4 5 6 7 8 9 10 11 1210 2 0.5 1.0 1.5 2.0 2.5 3.0 3.510 2 4 5 6 7 8 9 10 11 12Column : Shodex SB400 series, Shodex OHpak SB-800 HQ seriesEluent : H 2 OFlow rate : 0.3mL/min, 1.0mL/minDetector : (SB400) RI(small cell volume)(SB-800 HQ) RI(conventional type)Column temp. : Room temp.SEC analysis of humic substanceCalibration curve of Sodium polystyrene sulfonateHUMIC ACID(Dando soil)HUMIC ACID(Inogashira soil)FULVIC ACID(Dando soil)FULVIC ACID(Inogashira soil)7 9 11 13 15 17 minVoVtThe left graph shows the results of SECanalysis of reference materials (from theJapanese Humic Substances Society) ofhumic acid and fulvic acid prepared frombrown forest soil (Dando) and kurobokusoil (Inogashira).Sample : Humic substances, 30μLHumic acid 0.02mg/mLFulvic acid 0.02mg/mLColumn : Shodex OHpak SB-G + SB-805 HQEluent : 10mM NaH 2 PO 4 + 10mM Na 2 HPO 4 (pH7.0) + 25% CH 3 CNFlow rate : 0.8mL/minDetector : UV(260nm)Data provided by Nobuhide Fujitake,Column temp. : 40˚CProfessor of the Graduate School of Agricultural Science, Kobe University0Column : Shodex OHpak SB-800 HQ seriesEluent : 20mM LiBr in DMFFlow rate : 0.8mL/minDetector : RIColumn temp. : 40˚CCarboxymethylcelluloseMw=101 x 10 4Mn=10.5 x 10 4Mw/Mn=9.6Sample : Carboxymethylcellulose(Medium viscosity) 0.1%, 200μL2 4 6 8 10 12 14 16 18 20 22 24 26 28 30minColumn : Shodex OHpak SB-806M HQ x 2Eluent : 0.1M NaCl aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚C47Aqueous SEC (GFC) columns : Polymer-basedEffect of sodium nitrate concentration ineluent on the analysis of polyallylaminePolycarbonatePolyacrylamideIn the analysis of cationic polymers such as polyallylamine,the concentration of sodium nitrate in the eluent being0.02M would allow the column to adsorb the mainchain of polyallylamine, resulting in the completeprevention of the elution of polyallylamine. However, ifthe concentration is 0.1M or higher, adsorption of thesample is suppressed and a favorable chromatogramcan be obtained.Sample : PolycarbonateCH 3O C O CnCH 3O1.0x10 8SB-806 HQSample : Polyacrylamide, 100μLSB-807 HQSample : Polyallylamine 0.2%, 100μL0.1 M 0.02 M0.2 MMolar Mass(g/mol)1.0x10 71.0x10 6 6.0 8.0 10.0 12.0 14.0Mw: 5,500,0000.05 M1.0x10 54.0Volume(mL)8.012.0 16.0 20.0 24.0Volume (mL)8 12 16 20 minColumn : Shodex OHpak SB-806M HQ x 2Eluent : 0.5M Acetic acid + NaNO 3 aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex OHpakSB-805 HQ + SB-802.5 HQEluent : 5mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex OHpak SB-807 HQ, SB-806 HQEluent : 0.2M NaCl aq.Flow rate : 0.5mL/minDetector : RIMALS(Multi angle laser light scattering)Column temp. : 30˚C