Asahipak - Hplc.eu

Protein separation using QA-8251234Sample : 100μL1.Conalbumin 0.02%2.Transferrin 0.17%3.Ovalbumin 0.14%4.Trypsin inhibitor 0.17%Effect of eluent pH for DEAE-825pH6.0pH7.0314342210 5 10 15 min0 5 10 15 minSample : 100μL1. Conalbumin 0.06%2. Transferrin 0.27%3. Ovalbumin 0.33%4. Trypsin inhibitor 0.33%Standard analysiscolumnspH8.012 34pH9.01 432pH10.01,234015 30 min0 5 10 15 min 0 5 10 15 min 0 5 10 15 minColumn : Shodex IEC QA-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0)(B); (A) + 0.5M NaClLinear gradient; 100%(A) to 50%(A), 30minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Comparison data of porous DEAE-825 and non-porous DEAE3N-4TIEC DEAE3N-4T is a weak anion exchange column. Diethyaminoethyl group is on the surface of anon-porous gel. Rapid analysis of proteins and peptides are possible with non-porous packing materials.Its peak shape is still sharp, when a small volume of the sample is injected.1porous gel DEAE-82520 5 10 15 20 25 30 minColumn : Shodex IEC DEAE-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 60minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Injection vol. : 100μL3Column : Shodex IEC DEAE-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0), 20mM Tris-Trispropanol-HCl buffer(pH7.0)20mM Tris-HCl buffer(pH8.0), 20mM Ethanolamine-HCl buffer(pH9.0)20mM 1,3-Diaminopropane-HCl buffer(pH10.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 20minFlow rate : 1.0mL/minnon-porous gel DEAE3N-4T3: Diethylaminoethyl 1: DiethylaminoethylColumnEluent20 5 minFlow rateDetectorColumn temp. : Room temp.Injection vol. : 20μLSample :1. Conalbumin 0.4mg/mL2. Ovalbumin 1.2mg/mL3. Trypsin inhibitor 1.0mg/mL: Shodex IEC DEAE3N-4T: (A); 25mM Piperazine-HCl buffer(pH6.0)(B); A + 0.5M NaClLinear gradient; (A) to (B), 10min: 1.5mL/min: UV(280nm)Gibberellin IsomersHOHOOC2. GA14HOHOOC1. endo GA14CH3CH3COOHCOOH0 10 20 min12Sample :GibberellinColumn : Shodex Asahipak ES-502N 7CEluent : Acetic acid/H 2 O/CH 3 OH=0.1/0.4/99.5Flow rate : 1.5mL/minData was provided byDetector : UV(210nm)Prof. Yamaguch,Column temp. : 50˚CFaculty of Agliculture,University of Tokyo.25Columns for Anion Exchange ChromatographyMercaptoalbumins andnon-mercaptoalbuminsNucleotidesSulfide ion and cyanide ion123Sample :Normal control serum1. Globulin2. Uric acid3. HMA4. HNA12453678 9Sample :1. 5'CMP2. 5'UMP3. 5'TMP4. 3'CMP5. 5'ADP6. 3'UMP7. 5'GMP8. 2'AMP9. 3'AMP10. 3'GMP1Sample :1. HS - 0.1mg/L2. CN - 0.02mg/L24100 8 16 24 min 0 5 10 15 2025 min0.0 1.5 3.0 4.5 6.0 7.5 9.0 10.5 12.0 13.5 15.0 16.5 18.0minColumn : Shodex Asahipak ES-502N 7CEluent : 50mM N-methylpiperazine-HClbuffer(pH4.8) + 400mM Na 2 SO 4+ 0.3% C 2 H 5 OHFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 35˚CColumn : Shodex AXpak WA-624Eluent : 0.35M Ammonium acetate buffer(pH4.3)Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : 60˚CColumn : Shodex IEC DEAE-825Eluent : 10mM Na 2 CO 3 + 1mM Ethylenediamine+ 10% CH 3 OHFlow rate : 1.0mL/minDetector : EC(Electrode; Silver, 0mV SCE)Column temp. : 25˚C